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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Canine Parvovirus 2 VP2 Capsid Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: IgG was purified from sera taken from mice immunized with SARS-CoV-2 Spike protein (INO-4800) (23) using Nab protein G spin kit (ThermoFisher, 89949). 96-well half area plates (Corning ...
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bioRxiv - Microbiology 2021Quote: For analysis of the soluble protein fraction 50 µg protein were reduced with 2.5 mM TCEP (tris-(2-carboxyethyl) phosphine hydrochloride) (Invitrogen, Thermo Fisher Scientific) at 65 °C for 45 min before thiols were alkylated in 5 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The Z-scores for reactivity of SARS CoV-2 proteins to each human protein was determined by using ProtoArray Prospector v 5.2 software (Invitrogen, Thermo Fisher).
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bioRxiv - Cancer Biology 2023Quote: ... cells were harvested and lysed prior to splitting each sample into two aliquots for D-2-HG quantification and total protein quantification using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... HeLa cells seeded on cover glasses were transfected with plasmids encoding full length SARS-CoV-2 spike protein for 2 days using Lipofectamine 2000 (Thermo Fisher). Cells were fixed with 2% formaldehyde in PBS for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... and searched against the UniProt Human protein database and RefSeq SARS-CoV-2 protein database using Sequest HT through Proteome Discoverer (Version 2.2) (Thermo Scientific, Bremen, Germany). Precursor and fragment mass tolerance were set to 10 ppm and 0.02 Da ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were diluted to 2% (w/v) SDS and protein concentrations were determined using the Pierce BCA Protein Assay (Thermo Scientific, #23225). 20 μg of each lysate were subjected to an in-solution tryptic digest using a modified version of the Single-Pot Solid-Phase-enhanced Sample Preparation (SP3 ...
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bioRxiv - Microbiology 2021Quote: For analysis of the soluble protein fraction 50 µg protein were reduced with 2.5 mM TCEP (tris-(2-carboxyethyl) phosphine hydrochloride) (Invitrogen, Thermo Fisher Scientific) at 65 °C for 45 min before thiols were alkylated in 5 mM iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... and a 1:10 dilution of 2 μl of the samples was used to measure protein concentration by Qubit Protein Assay (Invitrogen, Cat# Q33211). The remaining 10 μl were reduced ...
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bioRxiv - Physiology 2023Quote: At day 45 (± 2 days) protein was extracted from beating iPSC-CMs in protein lysis buffer (1 X Pierce™ RIPA buffer (Thermo Fisher); 1 X PhosSTOP cocktail (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... The amount of affibody conjugated to the polymer was quantified by dissolving 2-3 mg of HA-Nor-Ox-Affibody in PBS at 2% w/v and determining the protein concentration of the solution using a Pierce 660 Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... Lysate (2 mg/mL in 200 μL) was precleared with 20 μL of protein Pierce protein A/G magnetic beads (Thermo Fisher, 88802). 5 μg of anti-KEAP1 (Santa Cruz Biotechnologies ...
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bioRxiv - Plant Biology 2024Quote: ... protein concentrations of fractions containing FtsZ1-2 and FtsZ2-1 were determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: ... we labeled 5-30 μM protein with 2 mM DTNB (Ellman’s reagent, Thermo Scientific) in 20 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein transfer was achieved using the iBlot™ 2 Gel Horizontal Transfer Device (Invitrogen). Nitrocellulose membranes were blocked in 5% BSA in PBS/Tween 20 (0.1% ...
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bioRxiv - Molecular Biology 2019Quote: ... Xpress-p85-A951E and Xpress-p85ΔRBS1/2 proteins were immunodetected using anti-Xpress (Invitrogen) antibodies ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were incubated for at least 2 hours with Dynabeads Protein A (Invitrogen 10001D) rotating at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... bound protein complexes were eluted in 40 µl 2× LDS buffer (Thermo Fisher Scientific) and fractionated on 7% NuPAGE Tris-Acetate gels (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... proteins were transferred onto a nitrocellulose membrane (iBlot™ 2 Transfer Stacks, ThermoFisher Scientific) blocked for 1h at RT with 5% Top-Block wt/vol in PBS supplemented with Tween-20 (10x PBS pH7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... The beads-protein complexes were isolated using a DynaMag-2 magnet (Thermo Fisher Scientific), applying 3 wash-resuspension cycles before short-term storage of the immunoprecipitate at −18 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were then transferred into a PVDF membrane (iBlot 2 Transfer Stacks, Invitrogen) and washed with Ponceau S red stain to confirm the successful transfer of proteins to the membranes ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S protein was conjugated to streptavidin-AF488 (Life Technologies #S11223), the HCoV-HKU1 S protein to streptavidin-BV421 (BD Pharmingen #563259 ...
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bioRxiv - Biochemistry 2022Quote: ... Bound proteins were eluted with 2 x SDS PAGE sample loading buffer (Thermo Fisher) and heating (95 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the purified protein was eluted using 2 µg/mL HA peptide (Thermo Scientific, 26184) in 50 mM HEPES ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were transferred onto a PVDF membrane using the iBlot-2 transfer system (Invitrogen). The membrane was washed and blocked with 5% BSA in Tris buffered saline with 0.05% Tween-20 (TBST ...
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bioRxiv - Biochemistry 2022Quote: ... Anti-sarcomeric protein α-Actinin 2 and ProLong Gold Antifade were purchased from Thermofisher Scientific (Waltham ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 2 µg/ml CD40 protein (ThermoFisher, A42565) in fresh PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were transferred to PVDF membrane using dry transfer method via iBlot 2 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... proteins were transferred to a nitrocellulose membrane (iBlot 2 Transfer Stacks, mini) in an iBlot 2 Gel Transfer Device (Invitrogen; Carlsbad, CA). Immunodetection was carried out using commercially available primary antibodies against PCCA (1:500 ...
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bioRxiv - Physiology 2019Quote: ... Cells were mechanically lysed by passing them 10 times with 301/2-gauge needle and small fractions were used to measure the total protein using a Coomassie protein assay (Thermo Fisher Scientific, #1856209). Cell lysate was incubated with Anti-FLAG M2 antibody for 1 h and immunoprecipitated (IP ...
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bioRxiv - Bioengineering 2023Quote: ... was used for separation of the proteins by molecular weight and then separated proteins were transferred in iBot 2 PVDF membrane (Thermo Fisher Scientific, USA). Anti-Epo polyclonal antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... An equivalent of 2×107 parasites whole protein lysate was loaded on a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen, cat log # NP0321BOX) followed by transfer onto a PVDF membrane (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were transferred to a PVDF membrane using the iBlotTM 2 Gel Transfer system (Invitrogen). After transfer ...
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bioRxiv - Molecular Biology 2021Quote: ... for 2 hours followed by 30 minutes incubation with protein A-coupled dynabeads (Invitrogen 10002D). Beads were washed three times with lysis buffer ...
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bioRxiv - Genomics 2020Quote: ... Proteins were transferred to a nitrocellulose membrane using an iBLOT 2 dry blotting system (ThermoFisher) and analysed by immunoblotting using CALNEXIN (Bethyl A303-696A ...
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bioRxiv - Biochemistry 2019Quote: ... The protein was run over two immobilized pepsin columns (Applied Biosystems; porosyme, 2-3131-00) stored at 10°C and 2°C at 200 µL/min for 3 min and the peptides were collected onto a VanGuard precolumn trap (Waters) ...
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bioRxiv - Cell Biology 2019Quote: ... 100 μM of the protein was incubated with 1 mM tris(2-carboxyethyl)phosphine (Invitrogen) for 30 min at RT protected from light ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit anti-S100 Protein Ab-2 (S100, 1:500, Thermo Scientific Cat# RB-044-A) was used to identify Schwann cells ...
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bioRxiv - Genomics 2022Quote: ... Mix 2 μg antibody with 20 μL Dynabeads Protein A beads (ThermoFisher Scientific, Cat. 10001D) with rotation at room temperature for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... and 55 nM of 2-Mercaptoethanol) with the protein transport inhibitor Brefeldin A (Thermo Fisher) added during the last 4 hrs of incubation at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were reduced and alkylated with 25 mM Tris(2-carboxyethyl)phosphine (TCEP) (Thermo Fisher) and 50 mM chloroacetamide (CAM ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were transferred onto PVDF iBLOT 2 Transfer Stacks according to manufacturer’s instructions (ThermoFisher Scientific). Membranes were blocked in 5% non-fat milk with 0.1% tween in PBS (PBS-T) ...
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bioRxiv - Molecular Biology 2019Quote: ... Approximately 2 mg of protein was mixed with 1x SUPERase·In™ RNase inhibitor (Invitrogen™) and incubated with Dynabeads IgG (11203D ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SARS-CoV-2 RBD protein was coupled to fluorescent beads (Thermo Scientific, Cat # F8819) by carbodiimide coupling ...
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bioRxiv - Microbiology 2020Quote: ... the purified SARS-CoV-2 RBD protein was coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at around 5μg/mL in PBS overnight ...
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bioRxiv - Plant Biology 2019Quote: ... A total of 2 µg of PageRuler Plus Prestained Protein Ladder (#26 619, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... the purified SARS-CoV-2 RBD proteins were coated on Nunc MaxiSorp immuno tubes (ThermoFisher) at 5μg/mL in PBS overnight ...
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bioRxiv - Microbiology 2021Quote: The protein samples were treated with Tris (2-carboxyethyl) phosphine hydrochloride (Thermo Fisher Scientific; # 20491) and 2-chloroacetamide (cat# 154955 ...
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bioRxiv - Immunology 2021Quote: ... immune complexes were incubated with magnetic beads for 2 h (Protein G Dynabeads, Thermo Fisher Scientific or Protein A/G magnetic beads ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were then further digested with 2 μg MS-grade trypsin (Thermo Fisher Scientific) per 100 μg protein for 20 hours at 37 degrees Celsius and 850 rpm ...