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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Canine Parvovirus 2 VP2 Capsid Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... protein quantification using BCA Protein Assay Kit (Thermo Fisher) was done ...
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bioRxiv - Microbiology 2020Quote: ... and proteins were immunoprecipitated with Protein A Dynabeads (ThermoFisher) conjugated to the relevant antibody (anti-MqsRA ...
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bioRxiv - Microbiology 2020Quote: ... Proteins were quantified using BSA Protein Assay Kit (ThermoFisher). Equal amounts of protein were boiled with 6X loading buffer (375mM Tris HCL ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentration was measured using BCA Protein Assay (ThermoFisher). For anti-NP immunoprecipitation ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations were determined with Bradford protein assay (Thermofisher), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... cas9 protein (Fisher Scientific A36498 Truecut Cas9 Protein v2) and gRNA were co-electroporated into G1 ES cells derived from an F1 hybrid blastocyst of 129S6 x C57BL/6J ...
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Allelic diversity uncovers protein domains contributing to the emergence of antimicrobial resistancebioRxiv - Microbiology 2022Quote: ... alongside protein standards (SeeBlue Pre-stained Protein Standard, Invitrogen). Gels were run at room temperature for 150 min at 80 V in running buffer ...
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bioRxiv - Plant Biology 2022Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and incubated at 4°C for 2 hours ...
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bioRxiv - Plant Biology 2023Quote: ... the magnetic Protein A and Protein G Dynabeads (Invitrogen) were added and mixture was rotated at 4°C for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... cas9 protein (Fisher Scientific A36499 TRUECUT CAS9 PROTEIN V2) and the gRNA with concentrations of 10 ug ...
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bioRxiv - Biochemistry 2023Quote: ... protein quantified by BCA protein assay ( Thermo scientific #23250), and 30 μg of the proteins were loaded on 4-12% Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD ...
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bioRxiv - Neuroscience 2023Quote: ... Protein concentration was determined by Qubit protein assay (Invitrogen) in a Qubit 4 fluorometer (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... 200 μL of Protein A (Dynabeads Protein A, Invitrogen) beads were washed twice in coIP buffer containing PIs ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, Cat # N13195) and incubated for 30 minutes in their respective culture conditions ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A-tagged proteins were detected with HRP-conjugated polyclonal anti-Protein A (Invitrogen, PA1-26853) at 1:5000.
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were resolved alongside a protein ladder (PageRuler Plus Pre-stained Protein Ladder, Thermo Fisher Scientific) on NuPAGE 4-12% BisTris gradient gels using NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was quantified using Qubit™ Protein and Protein Broad Range (BR) Assay Kits (ThermoFisher).
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bioRxiv - Microbiology 2023Quote: ... and protein concentration was determined by protein assay (EZQ Protein Quantification Kit; Life Technologies, Carlsbad, CA). Ct226-FLAG was affinity purified using magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μM 2-mercaptoethanol (2-ME, Gibco) and 1 × Antibiotic-Antimycotic (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... 2×2 binning (3.7mm^2 field area)) on the ArrayScan VTI (Thermo Scientific) and analyzed simultaneously for percentage of GFP positive cells using the Target Activation Bioapplication (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
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bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
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bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
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bioRxiv - Cancer Biology 2021Quote: ... Immunoprecipitation was performed by incubating 40 μL of Dynabeads Protein A coupled to 2 μg of anti-IgG2a or 2μg of anti-LXRα using bis(sulfosuccinimidyl)suberate (Thermo Fisher, UK; Cat. A39266) with 1 mg protein sample overnight at 4°C ...
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bioRxiv - Bioengineering 2019Quote: ... FAM-protein conjugates were twice purified to remove free FAM (Zeba™ 2 mL spin desalting columns with 40 kDa MWCO, Thermo Fisher Scientific) by washing with 0.1 M PBS three times (1,000 cfg for 2 minutes) ...
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bioRxiv - Genetics 2019Quote: ... SDS-Page separation was completed by running 2 μg of total protein on a NuPAGE™ 3-8% Tris-Acetate gel (Thermo Fisher Scientific). Next ...
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bioRxiv - Biophysics 2021Quote: ... were coated either with 200 ng of SARS-CoV-2 SΔTM proteins or bovine serum albumin (BSA, Thermo Scientific™, Waltham, MA, USA) through incubation overnight at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... These cells were cocultured with A549-ACE2-A2402 cells expressing each spike protein an E:T ratio of 2:1 and incubated with RPMI 1640 medium (Thermo Fisher Scientific, Cat# 11875101) containing 10% FBS at 37°C for 6 h ...
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bioRxiv - Immunology 2020Quote: The plasmid expressing the soluble pre-fusion version of SARS-CoV-2 S was used to produce the recombinant protein using the FreeStyle™ 293 Expression System (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... RNA-bound proteins were eluted from the rest 250 µL of beads by addition of 2 µg RNase A (Invitrogen, AM2271, 1µg/µL) in 30 µl Low Salt Buffer and rotation for 20 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 1000 μg of total protein was incubated overnight at 4 °C with 2 μg α-GFP (Life Technologies/ThermoFisher Scientific, Carlsbad, CA, USA) and 20 μL of a 50% slurry of sepharose Protein A (Rockland Immunochemicals ...
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bioRxiv - Biophysics 2020Quote: ... Subsequently, protein samples were washed once using Zeba™ Spin desalting columns (7 kDa MWCO, 2 mL) (Thermo Fisher Scientific, Waltham, MA, USA) equilibrated with degassed buffer A ...
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bioRxiv - Immunology 2021Quote: ... rVSV-ΔG/SARS-2-S*-luciferase pseudovirus was generated by transfecting BHK21/WI-2 cells with pcDNA expressing codon-optimized full-length SARS-CoV-2 S protein (GenBank accession number: MN908947) using Lipofectamine™ 2000 transfection reagent (Invitrogen, Carlsbad, CA). Transfected cells were then infected with rVSV-ΔG/G*-luciferase 24 h later ...
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bioRxiv - Molecular Biology 2020Quote: ... The elute was run 2 cm into a 1.5 mm NuPAGE™ Novex™ 4-12% Bis-Tris protein gel (Thermo Fisher Scientific), stained with Coomassie blue and distained with distaining solution (10% acetic acid and 50% methanol) ...
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bioRxiv - Molecular Biology 2021Quote: 2 × 20 μg of each of the three different SARS-CoV-2 spike proteins were mixed with 4x NuPAGE™ LDS sample buffer (Thermo Fisher Scientific) and up to 10 mM dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2022Quote: ... After a clarifying spin for 15 min at 14,000 ×g and 4°C 2 μl (MICOS complex) or 7 μl (all other mitochondrial protein complexes) G-250 Sample Additive (Invitrogen, Waltham, Massachusetts, USA) containing 40% glycerol were added ...
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bioRxiv - Cell Biology 2022Quote: ... Breg adenovirus-transduced brown adipocytes were cultured in serum-free medium with 1× protein transport inhibitor cocktail (10.6 µM Brefeldin A and 2 µM Monensin) (Fisher scientific, #50-930-9), 10 µM oligomycin ...
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bioRxiv - Immunology 2023Quote: Open-reading frames coding for either Nefmut alone or fused with SARS-CoV-2 N protein were cloned into pVAX1 plasmid (Thermo Fisher, Waltham, MA) as previously described [20] ...
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bioRxiv - Cell Biology 2023Quote: ... Click-iT reaction buffer additive 1 and Click-iT reaction buffer additive 2 from the Click-iT™ Protein Reaction Buffer Kit (Thermo Fisher Scientific) were added to the sample according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were probed with an anti-SARS-CoV-2 spike protein S1/S2 polyclonal antibody (1:1,000, Thermo Fisher Scientific PA5-112048), and an anti-p24 monoclonal antibody (1:1,000) ...
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bioRxiv - Bioengineering 2023Quote: ... For each sample 2 μg of solubilized silk protein was loaded into a NuPAGE™ 7% Tris-Acetate gel (EA03585BOX, Invitrogen, Waltham, MA) under reducing conditions ...
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bioRxiv - Genetics 2023Quote: ... Separated proteins were transferred onto a nitrocellulose membrane using an iBlot™2 Gel transfer device (cat. IB21001, Thermo Fisher Scientific, IL, USA). Non-specific protein binding to the membranes was blocked with 5% non-fat dry milk (cat ...
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bioRxiv - Biochemistry 2024Quote: ... The recombinant SARS-CoV-2 Spike S1-hFc-His tagged protein used for titrations and sorting was purchased from ThermoFisher (RP-876-79). The recombinant neuraminidase (NA ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations of the subcellular protein fraction lysates were measured using Pierce BCA Protein Assay Kit (ThermoFisher). Samples were boiled for 10 minutes and the extracts were separated by SDS-polyacrylamide gel electrophoresis using precast 4–20% Precise protein gels (ThermoScientific) ...
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bioRxiv - Neuroscience 2020Quote: ... Rab27b protein levels were normalized to total protein levels determined by SYPRO Ruby protein gel stain (Invitrogen).
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bioRxiv - Cancer Biology 2021Quote: The protein–protein interaction for endogenous protein was studied by using the Co-Immunoprecipitation Kit (Thermo Scientific). For overexpressed HA-tag proteins ...
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bioRxiv - Neuroscience 2023Quote: ... Synaptosomal protein expression levels were normalized to total protein using No-Stain Protein Labeling Reagent (Invitrogen, A44449). Primary antibodies (Anti-Shank3 (N367/62) ...