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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Canine Parvovirus 2 VP2 Capsid Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen, Waltham, MA, USA). 5 µg of each protein fraction was added to 1x loading buffer (250 mM Tris pH 6.8 ...
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bioRxiv - Neuroscience 2020Quote: Total protein concentrations of isolated protein lysates were quantified by BCA protein assay kit (ThermoFisher) and equal protein amounts were boiled at 95°C for 10 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific). Filter-aided sample preparation (FASP ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; ThermoFisher Scientific) and 0.01% pluronic acid (Merck ...
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bioRxiv - Cell Biology 2020Quote: ... recombinant proteins were diluted in EBC buffer and incubated with FAM111A antibody (2 μg/sample) coupled to Protein A agarose beads (Thermo Fisher Scientific) at 4°C for 2 hours on a rotary wheel ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µl of either type of samples were run on BOLT 4-12% Bis-Tris mini protein gels for SDS-PAGE and electroblotted using the iBlot 2 dry blotting system (Thermo Fisher, USA). Polyvinylidene fluoride membranes were probed with anti-PrP monoclonal antibody ICSM-18 (1:4,000 ...
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bioRxiv - Cell Biology 2021Quote: ... and by comparing band intensities of purified protein samples with a dilution series of 2 mg/mL Bovine Serum Albumin standard (Thermo Fisher 23209) on a Coomassie-stained SDS-PAGE gel ...
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bioRxiv - Cell Biology 2021Quote: ... 0.6 μg sgRNA was incubated with 3 μg SpCas9 protein for 10 minutes at room temperature and electroporated into 2×105 H9 cells using Neon transfection system (Thermo Fisher Scientific). Out of frame deletions were verified by DNA sequencing with Illumina MiSeq and by immunoblotting.
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bioRxiv - Microbiology 2020Quote: ... formic acid and the peptides equivalent to 2-4 µg of proteins were loaded for mass spectrometry analysis on a Orbitrap Elite mass spectrometer (ThermoFisher Scientific Inc.). The separation of peptides was performed on an analytical column (75 µm × 15 cm ...
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bioRxiv - Biochemistry 2022Quote: ... 500 μg of the supernatant was incubated with 2 μg of antibody followed by incubation with 10 μl 50% slurry of Protein A/G agarose (Thermo Fisher Scientific). After extensive washing ...
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bioRxiv - Microbiology 2022Quote: ... a plasmid encoding the EGFP-N fusion protein was constructed and transfected into infected HEp-2 cells with Lipo3000 (Thermo Fisher, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Plates were then washed twice with ELISA wash buffer to remove any excess unbound spike protein and blocked with 2% BSA (ThermoFisher Scientific; 37525) in ELISA wash buffer overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Sodium azide was removed from antibodies before use by diluting in 2 ml PBS and re-concentrating using a protein concentrator column (88521, Thermo Fisher Scientific) as this is critical for live imaging experiments using antibodies (Eilken et al. ...
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bioRxiv - Microbiology 2020Quote: TSA assays were performed by mixing 5 μL protein solution at 2 mg/mL with 5 μL SYPRO®Orange (1:50 diluted from 5000x stock solution, Invitrogen, USA), 5 μl of 8-fold buffer (1200 mM NaCl and 400 mM Tris pH 8) ...
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bioRxiv - Immunology 2020Quote: ... proteins were transferred from the gel onto a polyvinylidene difluoride (PVDF) membrane using an iBlot™2 Gel Transfer Device (ThermoFisher Scientific) at 20 V for 6 min ...
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bioRxiv - Immunology 2020Quote: ... was used as soluble substrate to detect SARS-CoV-2 S-protein specific monoclonal antibodies and the final reaction was measured by using the Varioskan Lux Reader (Thermo Fisher Scientific) at a wavelength of 405 nm ...
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bioRxiv - Immunology 2021Quote: ... HIV-based lentiviral pseudotyped particles harboring the SARS-CoV-2 Spike protein variants were produced using the ViraPower Lentiviral Expression system (Thermo Fisher Scientific) according to manufacturer’s protocol with minor changes ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 RDB protein was also coupled to DyLight 650 using the DyLight® Amine-Reactive Dyes kit (Thermo Fisher scientific).
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bioRxiv - Pathology 2021Quote: ... gels were incubated in 10% ethanol for dehydration before blotting the proteins to a membrane using the BlotTM 2 Gel Transfer Device (Thermo Fisher Inc.). After staining the membranes in Ponceau solution ...
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bioRxiv - Microbiology 2019Quote: ... We matched MS/MS fragmentation spectra to human forward protein databases and against reverse databases to a 1–2% false discovery rate using the SEQUEST database search program (Thermo Fisher Scientific) [61] ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The translation into liposomes was done as described previously95 and the output was analyzed by Blue Native PAGE using 2% digitonin and NativePAGE Novex 4-16% Bis-Tris Protein Gel (Thermo Fisher Scientific). For the in vitro mitochondrial protein import ...
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bioRxiv - Genetics 2020Quote: Proteins were extracted form 20 μL of ammonium bicarbonate resuspended fractions by adding 5 μL of lysis buffer (10% NP40, 2%SDS in PBS) and quantified by BCA protein assay kit (ThermoFisher Scientific, 23225).
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bioRxiv - Molecular Biology 2021Quote: ... for 14-17 h at 4°C followed by the incubation with 100 μl of Dynabeads Protein A + G (2:1 A:G proportion, Invitrogen, 10001D and 10004D) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: Both variants of the recombinant SARS-CoV-2 S protein (OptSpike1 and OptSpike2) were expressed in Expi293F™ and ExpiCHO-S™ cells according to manufacturer’s instructions (ThermoFisher Scientific). For Expi293F™ (ThermoFisher Cat# A14527 ...
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bioRxiv - Cell Biology 2020Quote: ... specimens were incubated with 10 nM purified recombinant POLArISact protein or 2-5 nM Alexa Fluor 488 Phalloidin (AF488-phalloidin, A12379, Thermo Fisher Scientific) and 10 mg/ml bovine serum albumin (BSA ...
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bioRxiv - Immunology 2021Quote: ... Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8, 150 mM NaCl) and 5x Sypro Orange (Invitrogen, stock 5000x) in a total volume of 20 μl ...
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bioRxiv - Molecular Biology 2020Quote: ... normalized to 2 mg/ml and protein expression was analysed by western blotting employing anti-V5 antibody (1:3000 dilution, Invitrogen, #R960-25).
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bioRxiv - Immunology 2020Quote: ... was used as soluble substrate to detect SARS-CoV-2 S-protein specific monoclonal antibodies and the final reaction was measured by using the Varioskan Lux Reader (Thermo Fisher Scientific) at a wavelength of 405 nm ...
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bioRxiv - Biochemistry 2021Quote: ... Usually 5 µg of total protein or the total protein of 1 to 2 × 106 autophagosomes were subjected to 4 – 12 % NuPage Bis-Tris gels (Thermo Scientific, MP0335) and transferred onto a nitrocellulose membrane using the Trans-Blot Turbo RTA mini nitrocellulose transfer kit (BioRad ...
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bioRxiv - Cell Biology 2022Quote: N2A cells were transfected as described above with BicD2-KIF tail fusions and MBNL-GFP proteins at a ratio of 2:3 in 4-well chamber slides (Thermo Fisher, 154526). 24 hours after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... for 2 hours and further digested using immobilised trypsin beads (40 µL of beads/250 µg of protein) (Thermo Fisher Scientific, 10066173) for 16 hours.
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of lysates were incubated with anti-53BP1 for 2 hours at 4°C and subsequently with Protein A magnetic beads (Thermo Fisher Scientific) for 1 hour at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The cell lysates containing an equal amount of total proteins (≃2 mg in 1ml RIPA buffer) were incubated with 15 µl (bed volume) of Pierce NeutrAvidin agarose (29200, Thermo Fisher Scientific) for 2 hours at 4°C to capture internalized biotinylated protein ...
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bioRxiv - Immunology 2023Quote: ... The supernatant was immunoprecipitated using 2 μg/ml anti-mouse ST2 antibody and Pierce™ Protein G Magnetic Beads (ThermoFisher, Cat# 88848), followed by western blot analysis.
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bioRxiv - Immunology 2023Quote: ... the microtiter plates were washed 5 × with PBS-T and then incubated for 1 hour at room temperature with 2 μg/ml of biotinylated recombinant protein G (PierceTM, Thermo Fisher Scientific) for recognition of both bat and human IgG antibodies ...
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bioRxiv - Immunology 2023Quote: ... Consensus and Vietnam matrix protein 2 (M2)-inducible HEK cells were generated using the Flp-In™ T-REx™ 293 Cell Line system (Invitrogen) and grown following the methods in the previous papers 53 ...
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bioRxiv - Immunology 2023Quote: ... 200-500 μm cystic EBs were selected under a dissecting microscope and transferred onto adherent tissue culture plates (∼2.5 EBs/cm2) for cultivation in Hematopoietic Differentiation (HD) medium (APEL 2, Stem Cell Tech, 05270; Protein Free Hybridoma, Thermo Fisher, 12040077 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.5 mL of a 1–2 mg/mL protein sample was mixed with 2.5 µL of SYPRO® orange dye (ThermoFisher Scientific, USA) and aliquoted at 5 µL before being mixed with 45 µL of the respective screening buffer ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated overnight with SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) rabbit polyclonal antibody (Thermo Fisher scientific, France) at a dilution of 1:1000 in the same buffer as for saturation ...
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bioRxiv - Cell Biology 2022Quote: ... followed by an incubation of 100 μl of a 2:1 (A:G) mixture of Dynabeads Protein A and G (Invitrogen, 10001D and 10004D) for 2h at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... The cells were then transfected with ancestral or variant SARS-CoV-2 spike protein encoding plasmid using Lipofectamine 3000 Transfection Reagent (L3000001; Thermo Fisher Scientific). After 24 hours ...
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bioRxiv - Biophysics 2023Quote: ... 0.5% NP-40 alternative) with protease inhibitors and cleared for 2 hours at 4 °C with magnetic Protein G Dynabeads (Thermo Fisher, #10009D) before overnight immunoprecipitation with 1 ug per 250ug of proteins of either normal serum IgGs or specific antibodies as listed above ...
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bioRxiv - Microbiology 2023Quote: ... AcGFP-JEVcore recombinant protein and Alexa Fluor 594 or 555-conjugated antibody (2 mg/mL, Thermo Fisher Scientific, as an injection marker) were microinjected into the cytoplasm at a final concentration of 1 mg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... Joachim Herz) for 2 h at 4°C followed by overnight incubation with 20 μL of protein G Ultralink resin (ThermoFisher Cat# 53128). The resins were washed with immunoprecipitation buffer and precipitated proteins were eluted with reducing SDS sample buffer ...
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bioRxiv - Microbiology 2024Quote: ... were transfected with the plasmid encoding the stabilized SARS-CoV-2 soluble S protein with the Expifectamine kit (GIBCO, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were transferred to nitrocellulose membrane at 20 V for 7 minutes using the iBlot™ 2 Transfer Stack (Thermo Fisher Scientific). Membranes were blocked in 5% non-fat milk powder in TBS+0.1% Tween (TBST ...
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bioRxiv - Cell Biology 2023Quote: ... 0.6 μg sgRNA was incubated with 3 μg SpCas9 protein for 10 min at room temperature and electroporated into 2×105 H9 cells using Neon transfection system (Thermo Fisher Scientific). Cells were put in a low O2 incubator and allowed to recover for 24-72 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Total proteins were quantitated using Qubit Protein Assays (ThermoFisher) and equal loads of total protein were separated on a NuPAGE 4-12% Bis-Tris mini gel (NP0336BOX ...
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bioRxiv - Neuroscience 2022Quote: ... BCA protein assay kit (ThermoFisher Pierce BCA Protein Assay) was used to determine the protein concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein was quantified by BCA Protein Assay (Thermo Scientific) and 50 µg of total protein in 100 mM TEAB and 10% acetonitrile was incubated at 99°C for 1 h.