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Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for rno mir 134 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... HEK-293T cells seeded at 2×105/well in 12 well plates and transfected with the indicated miR-722 overexpression and the RAC2 3’UTR reporter plasmids with Lipofectamine 3000 (Invitrogen). Cells were harvested and lysed at 48 h post transfection and luciferase activity was measured with Dual Glo-Luciferase reagent (Promega ...
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bioRxiv - Cancer Biology 2020Quote: ... PsiCHECK-2 - HOXA5-mut vectors were co-transfected respectively with miR-224 mimics or negative control using Lipofectamine 2000 (Invitrogen) in 24-well plates ...
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bioRxiv - Neuroscience 2022Quote: ... they became transfected with 80nM miR-124-3p mimics or scrambled (sc-miRNA) mimics (negative control) (Thermo) using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions (d1) ...
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bioRxiv - Bioengineering 2021Quote: ... Both normal PC-3 prostate cancer cell line (ATCC) and PC-3 transfected with miR-194 or negative control mimic were maintained in RPMI-1640 medium (Gibco) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Internal controls were present in Columns 13 and 14 of each library plate (Pre-miR miRNA Precursor Molecules Negative Control #1 - Ambion AM17110 ...
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bioRxiv - Cancer Biology 2019Quote: ... The recombinant vector and miR-659 mimic were jointly transfected into MDA-MB-231 cells using Lipofectamine™2000 (Invitrogen). These cells were lysed after 24 h of culture and the activity of luciferase was determined.
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bioRxiv - Cancer Biology 2019Quote: ... Precursors of respective human microRNAs (miR-27a, −122, −128, −193 and −217) and negative controls were purchased from Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... miR-325 and their controls (mirVana miRNA Mimic Negative Control #1 and Anti-miR Negative Control #1) were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2020Quote: ... were designed against the mouse Auts2 coding sequence using the BLOCK-iT Pol II miR RNAi Expression Vector Kit guidelines (Invitrogen). Auts2 miRNA constructs were subcloned into the pCL20c-trL7 ...
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bioRxiv - Microbiology 2021Quote: ... The pGL3-XIST-wt or XIST-mut and pmirGLO-SMAD2-wt or SMAD2-mut reporter vector were co-transfected with miR-34a mimics and NC mimics into SRA01/04 cells using Lipofectamine 2000 (Invitrogen). After 48h incubation ...
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bioRxiv - Cell Biology 2021Quote: ... LECs were transfected in 10 cm cell culture dishes with 20 nM miRVana mimic (miR-132 or negative control) using RNAiMAX (Invitrogen); two technical replicates each of two biological replicates i.e ...
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bioRxiv - Cancer Biology 2021Quote: ... and transfer plasmid (N174-CMV-GFP-miR-E-IRES-puro-shRNA) into HEK293T cells with Lipofectamine 2000 reagent (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: mirVana™ miRNA Mimics and Inhibitors were used for hsa-miR-181a-5p overexpression/inhibition (Ambion™ - 4464066 and 4464084). HeLa cells were transfected with corresponding mirVana™ miRNA in 24 well plates at a density of 20.000 cells per well ...
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bioRxiv - Cell Biology 2022Quote: ... The complementary DNA oligos were annealed to generate a double-stranded oligo and cloned into the linearized pcDNA6.2-GW/EmGFP expression vector (using the BLOCK-iT Pol II miR RNAi Expression Vector kit, Invitrogen, K493600). As a negative control ...
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bioRxiv - Molecular Biology 2023Quote: ... Quantitation of miR-423-5p expression levels was assessed using the Taqman™ MicroRNA Reverse Transcription kit (Thermo Fisher Scientific) and the specific Taqman™ MicroRNA Assay kit and the Universal PCR Master Mix No AmpErase UNG (both Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were transfected for 48 hours with 25 pmol per well (24-well format) of miRNA mimic Pre-miR miRNA precursors (Ambion, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... and hsa-miR-485-5p (catalog#17100, PM10837) or with 50 pmol per well (24-well format) of miRNA miRVana inhibitors (Ambion, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The miR-539-5p mimic or NC was cotransfected with the wild-type or mutant plasmid using lipofectamine 3000 (Invitrogen). Luciferase intensity was recorded 48 h after transfection using the dual-luciferase reporter assay system (Promega ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: RT-qPCR with TaqMan primer/probes was used to determine relative expression levels of miR-146a compared to U18 (human) or sno251 (mouse) endogenous control genes (ThermoFisher, assay IDs ...
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bioRxiv - Cancer Biology 2023Quote: ... both shβ7 cells and H295R cells were transfected with control miRNA mimics or hsa-miR-139-5p mimics (Thermofisher, #MC11749) at a final concentration of 10nM ...
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bioRxiv - Molecular Biology 2023Quote: ... Mice were co-injected with 10 μg of either a miR-146a mimic or a control miRNA sequence (ThermoFisher scientific). Mice were euthanized two weeks after intramuscular injection and muscles were harvested.
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bioRxiv - Microbiology 2024Quote: ... MCPyV ALTO variants were codon optimized to remove the LT and miR-M1 reading frame and synthesized as dsDNA fragments (IDT) and cloned into pcDNA3 (Invitrogen) or pTRIPZ (gift from Sandra Demaria – Addgene #127696 ...
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bioRxiv - Bioengineering 2022Quote: ... We coupled probes to the NHS-ester dyes Cy3 (for AXL, EGFR, and DDX58 probe sets) and Alexa Fluor 555 (Invitrogen, A-20009; for the GFP probe set). All conventional single-molecule RNA FISH probe sequences are available in Supplementary Table 5.
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bioRxiv - Microbiology 2020Quote: ... 200 U of Maxima RT (Thermo Scientific) and RNase inhibitor were added to the mix and further incubated 10 min at 25°C ...
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bioRxiv - Biochemistry 2021Quote: ... the Superscript™ II RT protocol (Invitrogen), the ORA qPCR Green ROX L Mix (HighQu ...
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bioRxiv - Neuroscience 2020Quote: ... RT mix contained 2 M betaine (Affymetrix), 2× Protoscript Buffer (New England Biolabs) ...
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Differential turnover of Nup188 controls its levels at centrosomes and role in centriole duplicationbioRxiv - Cell Biology 2019Quote: ... Superscript III RT kit (Thermo Fisher Scientific) was used to synthesize cDNA using Oligo-dT primers (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2019Quote: ... murine leukemia virus reverse transcriptase (RT; Invitrogen), and random hexamers (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2019Quote: ... Maxima H Minus RT (Thermo Fisher Scientific) and Triton X-100 was prepared and dispensed into the previously selected nanowells with single cells inside ...
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bioRxiv - Immunology 2021Quote: ... and 200 U Maxima RT (Thermo Scientific). RT reactions were incubated for 10 min at 25 °C ...
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bioRxiv - Microbiology 2021Quote: ... and finally 1µL SuperScript III RT (Invitrogen), 4µL of 5X First-Strand Buffer ...
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bioRxiv - Microbiology 2022Quote: ... + 1µL Superscript III RT (Invitrogen #18080-044) were added to the reaction and then the reaction was incubated at 45°C for 50 min ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... and reverse transcribed with MLV-RT (Invitrogen) as previously described (70) ...
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bioRxiv - Cancer Biology 2021Quote: ... and 200 U Maxima RT (Thermo Scientific). RT reactions were incubated for 10 min at 25 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... A Maxima H Minus RT (ThermoFisher EP0752) reaction was setup by mixing 40 μl of the T7 reaction with 30 μl 100 uM forward B primer ...
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bioRxiv - Genetics 2019Quote: ... cDNA was synthesized with MMLV-RT (ThermoFisher) using N15 oligonucleotides ...
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bioRxiv - Microbiology 2019Quote: ... Maxima RT (Thermo Scientific, 100 U/sample) and RiboLock RNase inhibitor (Thermo Scientific ...
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bioRxiv - Genomics 2020Quote: ... and 0.11uL of Maximus H-RT (ThermoFisher). For samples from ring gates we added 17.9uL of RT Master Mix (in the same proportions ...
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bioRxiv - Cell Biology 2020Quote: ... 15 U/μl Maxima H-RT (ThermoFisher), 1.65 U/μl RNasin (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... 1x Maxima H-RT buffer (Thermo Scientific) or 1x Kappa HiFi PCR buffer (Kapa Biosystems) ...
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bioRxiv - Immunology 2020Quote: ... cDNA was generated with Revertaid RT (ThermoFisher) and amplified by PCR using PfuTurbo DNA Polymerase (Agilent ...
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bioRxiv - Immunology 2021Quote: ... containing 2µl of Maxima RT buffer (ThermoFisher), 0.1 µl of 100 µM TSO oligo ...
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bioRxiv - Genomics 2020Quote: ... Maxima H Minus RT (Thermo Fisher Scientific) followed by 15 cycles of PCR using Takara LA Taq (Clontech ...
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bioRxiv - Immunology 2021Quote: ... cDNA synthesized with MMLV-RT (Life Technologies) was used to quantitate genomic viral RNA and mRNA levels of cytokines and chemokines (Power-Up SYBR-green master mix ...
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bioRxiv - Microbiology 2022Quote: ... and generated cDNA using Maxima RT (ThermoFisher). PCR amplicons were generated using SuperFi II PCR master mix ...
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bioRxiv - Microbiology 2022Quote: ... Taqman RT-qPCR multiplex assay (Applied Biosystems) was performed on a qTower (Analytic Jena ...
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bioRxiv - Cell Biology 2022Quote: ... and the Superscript II RT protocol (Invitrogen).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... M-MLV RT reagent purchased from Invitrogen. Primers are described in Table 1 (supplement).
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bioRxiv - Immunology 2022Quote: ... 20 U SuperScript IV RT (ThermoFisher Scientific), 20 U RNaseOUT (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2023Quote: ... 1X Maxima RT buffer (Thermo Scientific EP0751), 0.5 mM of each dNTP (Thermo Scientific R0192) ...