Labshake search
Citations for Thermo Fisher :
3901 - 3950 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... 2 μg of RNA was reverse transcribed into cDNA using the high-capacity reverse transcription kit (Applied Biosystems). cDNA and relevant primers were added to PowerUp SYBR green master mix (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2022Quote: ... cDNA was synthesized using 2 μg total RNA and High-capacity cDNA reverse transcription kit (Applied Biosystems, USA) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2020Quote: ... 2 mg of concentrated nanoparticles were labeled using an Alexa Fluor™ 647 Protein Labeling Kit (Thermo Fisher). The final degree of labeling (DoL ...
-
bioRxiv - Developmental Biology 2020Quote: ... 5-ethynyl-2’-deoxyuridine (EdU)) staining were performed using the Click-iT Plus EdU Assay kit (C10339; Invitrogen) according to the manufacturer’s instructions.
-
bioRxiv - Microbiology 2020Quote: ... 0.5 to 2 μg of RNA was reverse transcribed with the high-capacity cDNA RT kit (Applied Biosystems), using random hexamers for amplification of cellular and viral genes ...
-
bioRxiv - Genetics 2020Quote: ... cDNA was synthesized from 2 μg of total RNA using a cDNA Reverse Transcription Kit (Thermo Scientific, M1682). The cDNA was quantified by Real-Time PCR using SYBR Green master mix (YEASEN Biotech Co ...
-
bioRxiv - Zoology 2019Quote: Total RNA was extracted from 2-5 larvae using a commercial kit (TRIZOL, Life Technologies, Carlsbad, CA, USA), according to the manufacturer’s protocol ...
-
bioRxiv - Genomics 2020Quote: ... The extracted RNA (2 ug) was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Life Technologies). Quantitative PCR (RT-PCR ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2 μg of RNA was reverse transcribed into cDNA using the high-capacity reverse transcription kit (Applied Biosystems). The PowerUp SYBR green master mix (Applied Biosystems ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cell proliferation flow cytometry assays were performed using the 5-ethynyl-2’deoxyuridine (EdU) assay kit (Invitrogen #C10634). Cells were incubated with EdU for 24 h and labeled ...
-
bioRxiv - Cell Biology 2022Quote: ... 1-2 μg RNA was then converted to cDNA using high-capacity cDNA reverse transcription kits (Applied Biosystems). cDNA (1 ng/μL ...
-
bioRxiv - Developmental Biology 2022Quote: ... P15 or adult mice (2 months) were first quantified using a Pierce BCA Protein Assay Kit (Thermofisher; 23225) with absorbance read using a Glomax Explorer Microplate reader (Promega ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: Total RNA samples (2 μg) were reverse-transcribed with the high-capacity cDNA reverse transcription kit (Applied Biosystems), then analyzed with real-time quantitative polymerase chain reaction (qPCR) ...
-
bioRxiv - Molecular Biology 2023Quote: ... except replacing 2 x NTP / ARCA with individual NTPs from the MEGAscript™ T7 Transcription Kit (Invitrogen; AM1333). After IVT ...
-
bioRxiv - Microbiology 2022Quote: Total RNA (2 μg) was subjected to DNA removal using the TURBO DNA-free kit (Ambion, Life Technologies) following manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: Total RNA (2 μg) was subjected to DNA removal using the TURBO DNA-free kit (Ambion, Life Technologies) following manufacturer’s protocol ...
-
p110α-dependent hepatocyte signaling is critical for liver gene expression and its rewiring in MASLDbioRxiv - Cell Biology 2024Quote: ... Total RNA samples (2 µg) were reverse-transcribed using the High-capacity cDNA Reverse Transcription Kit (Applied Biosystems) for real-time quantitative polymerase chain reaction (qPCR ...
-
bioRxiv - Molecular Biology 2023Quote: ... 0.5–2 μg total RNA was used for reverse transcription (High-Capacity cDNA Reverse Transcription Kit, ThermoFisher Scientific) and subsequently miR-9-specific TaqMan assay (Applied Biosystems ...
-
bioRxiv - Molecular Biology 2024Quote: ... We reverse transcribed 2 μg of RNA into cDNA using the high-capacity reverse transcription kit (Applied Biosystems). cDNA was then processed for qPCR.
-
bioRxiv - Developmental Biology 2024Quote: ... RNA (2 μg) was reverse transcribed with a SuperScript III Reverse Transcription kit (Applied Biosystems, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... RNA (2 μg) was reverse transcribed with a SuperScript III Reverse Transcription kit (Applied Biosystems, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... RNA (2 μg) was reverse transcribed using the Applied Bioscience High-Capacity Reverse Transcription Kit (Thermo Fisher, #4368814). mRNA levels were determined using gene-specific primers by SYBR green nucleic acid labeling (Selleckchem PCR Mastermix ...
-
bioRxiv - Genomics 2024Quote: ... The extracted RNA (2 μg) was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Life Technologies). Quantitative PCR (qPCR ...
-
bioRxiv - Neuroscience 2023Quote: ... and mRNA (∼2 μg) was converted into cDNA using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). The expression of specific mRNAs was assayed using fluorescence-based quantitative real-time PCR (RT-qPCR ...
-
bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 coding sequences were inserted using the Gateway LR Clonase II Enzyme Mix kit (Invitrogen, CA), yielding plasmids pLCPD-S ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... sheared into pieces 1–2 kb in size and cloned using the TOPO Shotgun Subcloning Kit from Invitrogen. Colonies containing inserts were collected ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell lysis and cDNA generation was performed using the 2-Step Fast-Cells-to-CT- Kit (Invitrogen, ThermoFisher) according to their protocols ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell lysis and cDNA generation was performed using the 2-Step Fast-Cells-to-CT- Kit (Invitrogen, ThermoFisher) according to their protocols ...
-
bioRxiv - Cell Biology 2023Quote: ... Genomic DNA was extracted from murine C3H10T1/2 cells using the PureLink Genomic DNA Mini Kit (ThermoFisher Scientific).
-
bioRxiv - Molecular Biology 2023Quote: ... Fura-2 fluorescence was calibrated with defined Ca2+ buffers generated with a Calcium Calibration Buffer Kit #1 (Invitrogen).
-
bioRxiv - Microbiology 2023Quote: ... 0.002-2 ng/µL) or pEV samples according to the manufacturer’s instructions (Quant-iT PicoGreen dsDNA Kit; Invitrogen).
-
bioRxiv - Molecular Biology 2023Quote: ... Reverse transcription of 2 µg RNA was completed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) as per manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... cDNA was made from 1-2 µg of total RNA using the first strand cDNA synthesis kit (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... We next converted 2 ug of RNA to cDNA using a high-capacity RNA to cDNA kit (Thermofisher) and performed qPCR using GoTaq qPCR reagent ...
-
bioRxiv - Microbiology 2024Quote: ... Genomic DNA from 2 wells per sample was isolated using the PureLink Genomic DNA mini kit (ThermoFisher #K182001). The remaining MDMs were infected with HIV strain JFRL-mCherry at 10,000 pg of P24 per million cells by spinoculation at 1200 rpm for 2 hours at room temperature ...
-
bioRxiv - Microbiology 2024Quote: ... Genomic DNA from 2 wells per sample was isolated using the PureLink Genomic DNA mini kit (ThermoFisher #K182001). The remaining MDMs were infected with HIV strain JRFL-mCherry70–72 at 10,000 pg of P24 per million cells by spinoculation at 1200 rpm for 2 hours at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: Glucose uptake experiments were performed using 2-NBDG (2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose) (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
-
bioRxiv - Immunology 2024Quote: In vivo labeling of tumor-infiltrating T lymphocytes (TILs) with 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG, Life Technologies, catalog: N13195) was done by intravenous (retroorbital ...
-
bioRxiv - Cancer Biology 2019Quote: ... human recombinant IL-2 (2 ng/ml) (Gibco, USA), IL-7 (10 ng/ml ...
-
bioRxiv - Cell Biology 2021Quote: ... followed by 2 washes of 2× SSC (Ambion AM9765) for 5min ...
-
bioRxiv - Immunology 2020Quote: ... 2-mercaptoethanol (2◻×◻10-5 M, ThermoFisher), penicillin (100◻IU◻per ml ...
-
bioRxiv - Immunology 2023Quote: ... supplemented with 0.05 mM 2-mercaptoethanol (2-ME; Gibco). Mouse melanoma cell line B16 (ATCC ...
-
bioRxiv - Neuroscience 2023Quote: ... and 2 mM GlutaMax supplemented with 2% B27 (Gibco) and 5% horse serum (Hyclone) ...
-
bioRxiv - Bioengineering 2023Quote: ... and recombinant human interleukin-2 (IL-2) protein (Invitrogen) were purchased from Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2021Quote: ... 2-deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]- D-glucose (2-NBDG) (Invitrogen N13195) was used as a probe for monitoring glucose uptake ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... non-metabolizable glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Thermo Fisher Scientific, cat.no. 11569116). Samples were prepared as described in the section ...
-
bioRxiv - Cancer Biology 2021Quote: ... or 50 μg/mL neutralized rat tail collagen (rich in Coll I) (Gibco, A1048301) for 2 hours to overnight at 37 °C ...
-
bioRxiv - Neuroscience 2021Quote: ... and secondary antibodies (1:400, Alexa Fluor 633 goat anti-rat IgG, Thermo Fisher Scientific # A21094 ...