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Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... DNA quality was assessed using a 2% agarose gel and purified using the GeneJet PCR kit (Thermo Fisher Scientific). DNA was then eluted to a concentration of between 25-100 ng/µL and amplified using the T7 MegaShortScript RNA synthesis kit (Ambion ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA synthesis was performed with 2 μg RNA per sample using RevertAid First Strand cDNA Synthesis kit (Thermo Fisher). PR1 expression was detected using primers qPR1_F and qPR1_R by quantitative real-time PCR using Maxima SYBR green mix (Thermo Fisher ...
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bioRxiv - Neuroscience 2020Quote: ... Detection of EdU (5-ethynyl-2’-deoxyuridine) was performed as per the manufacturer’s guidelines using Click-it EdU Alexa Fluor 488 imaging kit (Invitrogen).
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bioRxiv - Cancer Biology 2020Quote: ... 2 µg of RNA were reverse transcribed using the Revert-Aid™ H Minus Reverse Transcriptase Kit (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Pathology 2019Quote: ... The complementary DNA was generated from 2 µg total RNA by RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific). Quantitative PCR (qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µL of tricolor calibration beads (Invitrogen™ MultiSpeck™ Multispectral Fluorescence Microscopy Standards Kit, Life Technologies Europe B.V.) was added to the sample ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were then lysed as per to manufacturer’s instructions with 4μl of lysis buffer (Cisbio phosphor-ERK1/2 cellular assay kit, Invitrogen, UK) for 30mins shaking at room temperature ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-2P trimer was biotinylated using the EZ-Link Sulfo-NHS-LC Biotinylation Kit (ThermoFisher) and tetramerized with streptavidin-APC (Agilent ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µL were used to measure protein concentration by a BCA protein assay kit (Thermo Fisher Scientific, Roskilde, Denmark).
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bioRxiv - Cell Biology 2020Quote: ... cDNA was prepared from 2 gg of total RNA using SuperScript™ III reverse transcriptase kit (ThermoFisher, 18080-044) with Oligo(dT)20 Primer (ThermoFisher ...
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bioRxiv - Genomics 2021Quote: ... The extracted RNA (1-2 μg) was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Life Technologies). Quantitative PCR was carried out in the BioRad CFX96 Real-Time System using iTaq Universal SYBR Green Supermix (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... baltica strain #1432 was extracted from 2 mL of overnight culture by Genomic DNA Purification Kit (Thermo Fisher Scientific) according to manufacturer’s protocol for Gram-negative bacteria ...
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bioRxiv - Cancer Biology 2021Quote: ... and 2 μg RNA was reverse transcribed into cDNA using a RevertAid first-strand cDNA synthesis kit (ThermoFisher, USA) supplemented with Oligo(dT)18 and Random Hexamer Primer ...
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bioRxiv - Immunology 2021Quote: ... Products were quantified by analyzing 2 μl with the Quant-iT dsDNA High-Sensitivity Assay Kit (Thermo Fisher, Q331120). Twenty ng of purified PCR product was used as input for library construction using the NEBNext Ultra II FS DNA Library Prep Kit (NEB ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of total RNA was determined (Supplementary Table 2) using the Qubit® RNA Assay Kit (Life Technologies). For rRNA depletion ...
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bioRxiv - Molecular Biology 2021Quote: ... 012-22384 Anti DYKDDDDK mouse IgG2b) using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (ThermoFisher). First ...
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bioRxiv - Genomics 2021Quote: ... SARS-CoV-2 detection was performed by quantitative real time PCR using Taqpath COVID-19 combo kit (Applied Biosystems, ThermoFisher Scientific ...
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bioRxiv - Genetics 2019Quote: Beginning at week 2 post cloning and continuing until week 6 the Phusion Blood Direct PCR Kit (Thermo Scientific) was utilized to identify positive clones ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, ThermoFisher Scientific) according to the manufacturer protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 µg of RNA was reverse-transcribed using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, ThermoFisher Scientific) according to the manufacturer protocol ...
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bioRxiv - Neuroscience 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) was incubated using Click-iT™ Plus EdU Cell Proliferation Kit (Thermo Fisher Scientific) after DAPI staining ...
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bioRxiv - Genomics 2022Quote: ... The amplified products were checked on 2% agarose electrophoresis and purified using Purelink PCR purification kit (Invitrogen, United States). The purified amplicons were quantified on Qubit 2.0 using qubit dsDNA BR assay kit (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... Full length genomic SARS-CoV-2 RNA was in vitro transcribed using mMESSAGE mMACHINE T7 Ultra Transcription kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: ... putida from 2 mL of overnight LB medium cultures with the PureLink Genomic DNA Mini kit (ThermoFisher Scientific Co.). Library construction ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 2 µg of total RNA using a TRUE Script Reverse Transcription Kit (+gDNA Eraser) (Invitrogen). Quantitative real-time PCR was performed on an ABI Quant Studio™ 6 Flex system (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of total RNA was used to synthesize cDNA using the High Capacity Reverse Transcriptase kit (Applied Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of total RNA was used to synthesize cDNA using the High Capacity Reverse Transcriptase kit (Applied Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Full length genomic SARS-CoV-2 RNA was in vitro transcribed using mMESSAGE mMACHINE T7 Ultra Transcription kit (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was purified from 2 ml of cultures using the GeneJET Genomic DNA Purification Kit (K0722, Thermo Scientific) in accordance with manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... Fura-2 fluorescence was calibrated with defined Ca2+ buffers generated with the Ca2+ Calibration Kit #1 (Invitrogen, Waltham, USA).
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bioRxiv - Plant Biology 2023Quote: ... determined by qPCR with the KAPA kit (Biosystems. Boston, MA, USA) and confirmed with a 2-point Qubit 2.0 fluorometer (Invitrogen). The library was denatured with 10 µL of 0.2N NaOH and diluted in a solution of denatured PhiX ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 3’ end RNA was synthesized by in vitro transcription using an mMESSAGE mMACHINE T7 kit (Invitrogen) with a DNA template (5’- TAATACGACTCACTATAGCTATCCCCATGTGATTTTAATAGCTTCTTAGGAGAAUGACGTAGCATGCTACGCG -3’ ...
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bioRxiv - Molecular Biology 2022Quote: ROS levels were detected using the chloromethyl-2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) general oxidative stress indicator kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthesized from 2 µg of RNA template using the High Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems). For the qPCR reaction mixture KAPA SYBR FAST Master Mix (2x ...
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bioRxiv - Immunology 2024Quote: ... RNA (2 μg) was reverse transcribed using random primers with a RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific). Quantitative PCR was performed in triplicate using the SYBR Green method (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNAs were prepared from 2 μg RNA with random hexamers using the High-Capacity cDNA Synthesis Kit (Thermo Scientific), followed by RT-PCR to identify the BCR::ABL1 fusion transcripts using a previously described protocol (17) ...
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bioRxiv - Bioengineering 2023Quote: ... Tris (2-carboxyethyl) phosphine hydrochloride (TCEP.HCl) and Vybrant™ CFDA SE Cell Tracer Kit were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2023Quote: ... cDNA synthesis from 1.5 or 2 μg RNA was performed using the High Capacity cDNA RT Kit (Applied Biosystems). Quantitative RT-PCR experiments were run on a C1000 Touch Thermal Cycler (BioRad ...
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bioRxiv - Neuroscience 2023Quote: ... 2 µg of the total RNA was converted into cDNA using the SuperScript II Reverse Transcriptase kit (Invitrogen™). qPCR was performed on CFX384 Real- Time PCR Detection System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was prepared with 2 µg of total RNA using RevertAid first strand cDNA synthesis kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.01% [v/v] 2-mercaptoethanol) and then quantified with the Pierce BCA protein assay kit (Thermo Fisher Scientific). The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2024Quote: The cDNA samples were synthesized from 2 µg of RNA using a High-capacity cDNA synthesis kit (Applied Biosystems). RT-qPCR was performed using the ABI SYBR green kit as per manufacturer’s recommendation ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was prepared from 2 µg RNA using the High-capacity cDNA Reverse Transcription kit (Applied Biosystems, Cat# 4368813) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2024Quote: ... 2 µg of RNA was used for cDNA synthesis using a high-capacity cDNA reverse transcription kit (Applied Biosystems). PCR products were checked using Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Microbiology 2020Quote: ... ELISA plates were blocked using PBS supplemented with 3% bovine serum albumin (PBSA; Thermo Fisher Scientific, Waltham, MA). THL-treated virus was added to blocked KZ52-coated plates and allowed to adsorb at 37°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed five times before incubation with 1x TMB ELISA substrate solution (eBioscience; Thermo Fisher Scientific, Inc.) and the reaction was stopped with 1 M H2SO4 ...
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bioRxiv - Neuroscience 2020Quote: ... Human phospho-tau [pT181] was measured using a commercially available ELISA per manufacturer’s instructions (Thermo Fisher Scientific, KHO0631). Guanidine extracted supernatants of hippocampus homogenates described above were utilized for the pTau ELISA.
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bioRxiv - Neuroscience 2020Quote: The level of Aβ in the tissue was assessed by ELISA as per the manufacturer’s instructions (KHB3442, Invitrogen). The resulting measurements were normalized for tissue weight.
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bioRxiv - Microbiology 2022Quote: Headless HA ELISAs were performed using 96-well Immunlon 4HBX flat-bottom microtiter plates (no. 3855; Thermo Fisher) coated with 0.5 ug/well of streptavidin (no ...
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bioRxiv - Neuroscience 2021Quote: ... were prepared for ELISAs by coating each well with 50ul of 1.5ug/ml HT7 monoclonal antibody (Thermo Fisher) the night before use ...