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Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... Control animals were treated with the same dosage of rat IgG isotype (Invitrogen #10700). The organs were harvested 2 or 24 hours after IR injury ...
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bioRxiv - Microbiology 2023Quote: ... and Alexa Fluor 546-conjugated goat anti-rat IgG (1:2000, Invitrogen, A-11081) were used as secondary antibodies ...
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bioRxiv - Immunology 2023Quote: ... were exposed to appropriate secondary IgG: chicken anti-rat IgG-Alexa Fluor 647 (Invitrogen), donkey anti-goat IgG-FITC (Rockland ...
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bioRxiv - Immunology 2023Quote: ... and goat anti-rat Alexa Fluor-555(Cat#A-21434, RRID:AB_141733, Invitrogen, 1:200) were used as secondary antibodies appropriately ...
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bioRxiv - Neuroscience 2023Quote: ... Antibodies used were: goat anti-rat Alexa fluor 488 (1:250, A11006, ThermoFisher Scientific), goat anti-mouse Alexa fluor 568 (1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... we used: mouse monoclonal anti-rat pThr181 (1:1000, Invitrogen Life Technologies, Carlsbad, CA), mouse monoclonal anti-rat pSer202/pThr205 (AT8 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal anti-rat pSer202/pThr205 (AT8, 1:1000, Invitrogen Life Technologies, Carlsbad, CA), mouse monoclonal anti-rat pThr231 (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... we used: mouse monoclonal anti-rat pThr181 (1:1000, Invitrogen Life Technologies, Carlsbad, CA), mouse monoclonal anti-rat pSer202/pThr205 (AT8 ...
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bioRxiv - Neuroscience 2023Quote: ... and Ly006C+ cells were depleted using Dynabead anti-rat (Cat. #: 11035, Invitrogen Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and Ly006C+ cells were depleted using Dynabead anti-rat (Cat. #: 11035, Invitrogen Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal anti-rat pSer202/pThr205 (AT8, 1:1000, Invitrogen Life Technologies, Carlsbad, CA), mouse monoclonal anti-rat pThr231 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... followed by secondary mouse-absorbed Alexa 488-conjugated goat anti-rat antibody (Molecular Probes) diluted 1:50 ...
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bioRxiv - Cell Biology 2024Quote: ... or rat secondary antibodies conjugated with Alexa Fluor 488 or 555 (Thermo Fisher Scientific). Slides were mounted with antifade mounting medium and DAPI (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... Secondary antibody (Goat anti-rat Alexa Fluor 488, Invitrogen #A-11006, 1:300 dilution) and nuclear stain (DRAQ5 ...
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bioRxiv - Developmental Biology 2024Quote: ... anti-rat 568 secondary antibody (H+L) 1:400 (Thermo Scientific, Alexa Fluor 568)] ...
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bioRxiv - Neuroscience 2024Quote: ... and Donkey anti-Rat IgG Alexa Fluor 488 (1:500; Thermo Fisher; RRID: AB_2535794). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... and AlexaFluor 647 goat anti-rat IgGs (RRID: AB_141778; 1:250) (Invitrogen, Carlsbad, CA).
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bioRxiv - Cell Biology 2024Quote: ... donkey anti-rat IgG (H+L) Alexa Fluor 647 (Invitrogen; A48272; IF, 1:500), camelid anti-rabbit IgG Abberior STAR 635P FluoTag-X2 (Abonva ...
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bioRxiv - Cell Biology 2024Quote: ... The secondary antibodies we used were Donkey anti-rat Alexa 405 (Thermo Fisher, A48268), donkey anti-mouse Alexa 555 (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... donkey anti-rat IgG conjugated to Alexa 594 (Thermofisher, Cat# A-21209, 1:600), and donkey anti-goat IgG conjugated to Alexa 647 (Thermofisher ...
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bioRxiv - Neuroscience 2024Quote: ... goat α-rabbit and goat α-rat IgG secondary antibodies (Molecular Probes; Jackson Immunoresearch) were used at 1:500.
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bioRxiv - Immunology 2024Quote: ... Secondary antibody information: Donkey anti-rat 647 (1:1000, Cat# A48272, Invitrogen, California, USA); Donkey anti-rat 568 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... monoclonal rat: anti-Ki67 (1:500, Cat. No. 14-5698-82, Thermo Fisher Scientific); polyclonal rabbit ...
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bioRxiv - Microbiology 2020Quote: 50µg of peptides from each sample were labeled with isobaric tandem-mass-tag (TMT) 11 plex reagents (Thermo Fisher Scientific, San Jose, CA) in 20 mM pH 8.5 HEPES with 30% acetonitrile (v/v ...
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bioRxiv - Neuroscience 2021Quote: ... After washing the sample with washing buffer (PBS, 1% Bovine Serum Albumin (BSA), cells were centrifuged (10 min, 150g, RT) and stained for microglia marker CD11b (Invitrogen, 11-0112-81) (1:100) ...
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bioRxiv - Microbiology 2022Quote: ... the cells were stained overnight at 4 °C with E1 or nsP1 primary rabbit antibodies (both antibodies previously validated in our laboratory) [11] followed by Alexa Fluor 568 goat anti-rabbit secondary antibody (Invitrogen, Carlsbad, CA, USA). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... HPCs in suspension were collected and spun down at 300 xG for 6 minutes and resuspended into microglia basal media (MBM, 2mL/well) containing: DMEM/F12 no phenol (Gibco # 11-039-021), 2% Insulin Transferin Selenite (Gibco # 41400045) ...
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bioRxiv - Physiology 2021Quote: ... Treated RNA (11 μL per sample) was used to produce cDNA by means of the SuperScript III Reverse Transcriptase (Life Technologies, Carlsbad, US) and a 1:1 mix of Random Hexamers and 10 μM Oligo(dT ...
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bioRxiv - Immunology 2021Quote: ... at 1/100-200 dilution at RT for 1-2h in dark humidified incubation chamber or isotype control antibodies including Rabbit IgG-FITC (Invitrogen, 11-4614-80), Armenian Hamter IgG-PE (Invitrogen ...
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bioRxiv - Physiology 2021Quote: ... gestation day between E9-11) were isolated and the tissue was fragmented into pieces followed by incubation in Trypsin-EDTA (0.05 %) (Thermo Fisher Scientific, Waltham, USA) for 15 min at 37°C ...
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bioRxiv - Genetics 2020Quote: ... The cells were incubated at 37°C for up to 20 min (day 11) or up to 35 min (days 30 and 52) before adding DMEM:F12 (Thermo Fisher Scientific, 10565-018) supplemented with 10 μM Rock inhibitor and 33 μg/mL DNase I (Worthington ...
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bioRxiv - Immunology 2022Quote: ... and cells were flushed out from the cavity into RPMI medium-1640 (Cat. 11-875-093; Gibco, Thermo Fisher, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Cat ...
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bioRxiv - Immunology 2022Quote: ... and cells were flushed out from the cavity into RPMI medium-1640 (Cat. 11-875-093; Gibco, Thermo Fisher, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Cat ...
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bioRxiv - Microbiology 2022Quote: ... and cells were fixed by using a fixing solution prepared by adding 4 ml of glutaraldehyde and 11 ml of formaldehyde to 500 ml of Dulbecco PBS (Thermo Fisher Scientific #14190144). After 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... while the human pancreatic cancer cell line AsPC-1 (passage 11) was cultured in RPMI-1640 medium (L-glutamine supplement; Gibco, Thermo Fisher Scientific), both supplemented with 10 % (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... and the plasmid pcDNA3-hLOXL2 was used to amplify the SV40Pr-Neo-term sequence (primers 11-12) using the Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific; #F530). These two PCR products were cloned into the digested repair vector GW209_pCRIS-PITChv2-C-dTAG-Puro (BRD4 ...
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bioRxiv - Biochemistry 2023Quote: ... 500 µL of perfusate was dried in 0.5 mL V-vials and reconstituted in 50 μL of 20 mg/mL hydroxylamine hydrochloride (CAS# 5470-11-1; Acros organics, New Jersey) in pyridine (CAS#25104 ...
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bioRxiv - Immunology 2023Quote: Another set of harvested lungs and spleen immune cells were stained with recommended dilutions of antibodies conjugated to fluorophores: CD3-FITC (Invitrogen, #11-0032-82) CD4-SB600 (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: Human osteosarcoma cell line U2OS (ATCC-HTB-96) and human colorectal carcinoma cell line HCT116 (ATCC-CCL-247) were cultured in DMEM (Gibco, 11-965-092) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2023Quote: ... cut horizontally to separate EZH2 and beta-actin and then incubated at 4°C with primary EZH2 antibody (ThermoFisher, Cat. No. #49-1043; 1: 1.000) and anti-beta actin antibody (Sigma-Aldrich-A2228-RRID:AB_476697 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... 0.5 to 2 µg of RNA was reverse transcribed with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using random hexamers for amplification of cellular genes or gene specific primers for amplification of either minus or plus strand viral RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNA was synthesized from 2 μg of total RNA using a High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). The expression of 370 ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA (2 µg) was then reverse transcribed with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems; ThermoFisher Scientific). RT-qPCR was then performed via the CFX384 Real Time system (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA (2 µg) was then reverse transcribed with the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems; ThermoFisher Scientific). RT-qPCR was then performed via the CFX384 Real Time system (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 µg of the extracted RNA were reverse-transcribed using the high capacity cDNA reverse transcription kit (Applied Biosystems) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... using a 1:2 mass ratio of light and heavy chain DNA with the Expifectamine transfection kit (Life Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... 0.5 to 2 µg of RNA was reverse transcribed with the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) using random hexamers for amplification of cellular genes or gene specific primers for amplification of either minus or plus strand viral RNA ...
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bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription was performed using first-strand cDNA synthesis kit containing SuperScriptIII® (2 units/µL; Invitrogen, Life Technologies).
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bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription was performed using first-strand cDNA synthesis kit containing SuperScriptIII® (2 units/µL; Invitrogen, Life Technologies).