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Citations for Thermo Fisher :
3801 - 3850 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... 2% (ThermoFisher). Purified amplicons were quantified by Qubit™ dsDNA Quantification Assay Kits (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... were co-transfected with the EGFP-Caspase 2 or EGFP-Δ Caspase 2 plasmid and the synthesized miR-2 (miR-2-mimic) using Cellfectin II transfection reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Macrophages were incubated with the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (10 μM, Invitrogen, California, USA) in PBS for 30 min ...
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bioRxiv - Immunology 2021Quote: ... containing 2’-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 50 μM; Thermo Fisher Scientific). To determine FA uptake ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in L6 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in Caco2 cells ...
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bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... stained with Ghost-510 and 2-(N-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 60 µM) (Thermo Fisher Scientific) (30 min at 37 C) ...
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bioRxiv - Cell Biology 2020Quote: Cells were treated with the siRNA pool targeting FCHSD2 (#J-021240-10, J-021240-11, J-021240-12, Dharmacon) or Rab7 (LU-010388-00, Dharmacon) using RNAiMAX (Thermo Fisher Scientific) to silence the endogenous protein ...
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bioRxiv - Genetics 2021Quote: ... Differentiation efficiency was quantified using flow cytometry as described previously 59 using mouse monoclonal antibody against cardiac troponin-T (clone 13-11, Thermo Fisher Scientific). All iPS-CM cultures used in this study were >85% cTnT+ ...
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Single-cell analysis of skeletal muscle macrophages reveals age-associated functional subpopulationsbioRxiv - Cell Biology 2022Quote: ... Pellet 1 was subjected to a second round of digestion in 1 mL of 1000 U/mL Collagenase type II and 1 mL of 11 U/mL Dispase II (Thermofisher, Cat# 17105041) along with the 8 mL of the remaining cell suspension ...
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bioRxiv - Immunology 2019Quote: ... 11-7139-42 or 85BRD, cat.: 11-7136-42), IL-4-PE (8D4-8, cat.: 12-7049-42) (all from Thermo Fisher Scientific); TNFa-Pacific Blue (MAb11 ...
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bioRxiv - Cancer Biology 2020Quote: ... MV4-11 (acute monocytic leukemia M5) and OCI AML3 (acute myelomonocytic leukemia M4) were maintained in RPMI 1640 (Gibco, Thermo Fisher Scientific) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2020Quote: ... MV4-11 (acute monocytic leukemia M5) and OCI AML3 (acute myelomonocytic leukemia M4) were maintained in RPMI 1640 (Gibco, Thermo Fisher Scientific) supplemented with 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were digested with the filter-aided sample preparation (FASP) method23 and peptides were labeled using TMT 11-plex isobaric reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Flow cytometry analysis to measure parasitemia at the cycle of drug exposure was measured using the nucleic acid stain SYTO 11 (0.016 μM) (Life Technologies no. S7573) and a BD FACSCalibur machine as previously described (Rovira-Graells et al ...
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bioRxiv - Cancer Biology 2021Quote: ... For DHT stimulation experiments cells were starved of hormone for 48 hours in phenol red-free RPMI media (Gibco, 11-835-030) with 10% charcoal stripped FBS (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... The homogenate was washed HBSS four times and islets were handpicked into islet media-G11 {RPMI-1640 media with 11 mM glucose (Gibco, Dublin, Ireland) plus 10% heat-inactivated Fetal Bovine serum [HI-FBS] (Atlanta Biologicals ...
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bioRxiv - Neuroscience 2020Quote: ... and then the stylet was detached at the proximal end using a scalpel (Feather disposable scalpel, No. 11, Fisher Scientific #FH/CX7281A). The severed end was immediately placed in the silicone lubricant with the stylet tip facing the center of the coverslip ...
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bioRxiv - Cell Biology 2020Quote: ... and HeLa FlpIn Cells (Etemad, Kuijt, & Kops, 2015) cells were grown in a high glucose DMEM (Thermo Fisher Scientific 11-965-118/Gibco 11965118) supplemented with anti/anti (Thermo Fisher Scientific 15240062 ...
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bioRxiv - Microbiology 2022Quote: ... 11 mL cultures were grown at 37°C without shaking (HeraTherm™General Protocol Microbiological Incubator, Thermo Fisher Scientific, Waltham, MA, USA) in Balch tubes with N2/CO2 (80/20 ...
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bioRxiv - Pathology 2022Quote: ... Northern blotting was conducted as our previously reported method [66] with biotin-labeled DNA probes using Biotin-11-dUPT (Thermo Scientific USA). High-molecular weight RNAs were separated in 1 % formaldehyde agarose gels and transferred to Hybond-N+ membranes (GE Healthcare ...
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bioRxiv - Molecular Biology 2022Quote: For one sample of selected RGCs two dissected retinas were dissociated as previously described 58,11 and stained with CD90.2(Thy-1.2)-FITC antibody (Thermo Fisher REF#11-0902-81). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Quantitative RT-PCR was conducted with gene-specific primers (refer to Supplementary Table 11) using either THUNDERBIRD Next SYBR qPCR Mix (TOYOBO) or TaqMan assays (Thermo Fisher Scientific) with TaqMan Universal Master Mix II ...
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bioRxiv - Molecular Biology 2024Quote: ... An expression backbone originally containing wild-type Cas7-11 (pDF0506) was digested with Fermentas FD0596 NotI and Fermentas FD1464 AgeI (Thermo Fisher Scientific) with 10x FastDigest Buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... peptides were resuspended in 10 µL of water and labeled for 1 h at room temperature with 4 µL (∼11 µg/µL in acetonitrile) of TMT10plex or TMT18plex (Thermo Fisher Scientific). The reaction was quenched with 4 µL of 5% hydroxylamine ...
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bioRxiv - Pathology 2024Quote: Sex-matched 9 to 11-week-old wild-type TGFβ1-Tg+ mice received 0.5 mg/mL doxycycline hyclate (Thermo Scientific Cat No. J60579.22) in their drinking water for 21 days as previously described [35].
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bioRxiv - Microbiology 2024Quote: ... The concentration and quality of DNA were evaluated by NanoDrop DS-11+ Spectrophotometer (DeNovix, Wilmington, U.S.) and Qubit 2.0 Fluorometer (Thermo Fisher Scientific, Massachusetts, U.S.). From each sampling site ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific) and cultured at a density of 3-5×106 cells/ml in a 37°C incubator with ≥80% relative humidity and 8% CO2 on an orbital shaker at 125×g for 3-4 days ...
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bioRxiv - Neuroscience 2023Quote: ... Briefly, cortices were dissociated, and diluted in 10% Fetal Bovine Serum (OmegaSci, FB-11)/1% penicillin-streptomycin in DMEM (Gibco, 11995-065) and plated at a density of one brain per uncoated T75 flask ...
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bioRxiv - Biochemistry 2023Quote: ... only the early fractions that contained the 40S peak to avoid eIF3-bound 40S subunits) were pooled and pelleted in a 11 x 34 mm thin-wall polycarbonate tube (Thermo Fisher # 45315) at 55,000 rpm for 18 hrs at 4°C in a Sorvall S55-S rotor using a Sorvall Discovery M120 SE Micro-Ultracentrifuge ...
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bioRxiv - Cancer Biology 2022Quote: ... while the human pancreatic cancer cell line AsPC-1 (passage 11) was cultured in RPMI-1640 medium (L-glutamine supplement; Gibco, Thermo Fisher Scientific), both supplemented with 10 % (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-APP (22C11, 1:1000 dilution), Anti-β Amyloid (D-11 Santacruz, Cat. Sc-374527, 1:1000) and anti-β-tubulin (Invitrogen Cat.32–2600 or Proteintech Europe ...
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bioRxiv - Neuroscience 2023Quote: ... in electron-event representation mode with a flux of 11 electrons/pixel/s and a Selectris-X energy filter (Thermo Fisher Scientific) with a slit width of 10 eV to remove inelastically scattered electrons ...
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bioRxiv - Neuroscience 2023Quote: ... HPCs were converted into homeostatic microglia with a differentiation medium composed of DMEM/F12 basal media (Gibco, Catalog No. 11-320-033), 2X 100X Insulin-Transferrin-Selenium (ITS-G)(Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Fluorescence measurements (Ex 480 nm, Em 520 nm) were obtained with a BioTek Synergy H1 microplate reader (Fisher Scientific, 11-120-536) at 37 °C ...
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bioRxiv - Genetics 2024Quote: ... DNA concentration was determined by either DeNovix DS-11 Spectrophotometer (DeNovix Inc., Wilmington, Delaware, USA) or Qubit 3.0 Fluorometer (Thermo Fisher Scientific Inc.). All sampled animals were privately owned pet dogs enrolled in the study with the owner’s informed consent ...
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bioRxiv - Neuroscience 2024Quote: ... Cultures were harvested after 10-11 days incubation with lentivirus and samples were prepared for western blot using RIPA buffer (Thermo Fisher, 89900). Samples were heated for 10 minutes at 95° C with 50 mM DL-dithiothreitol (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... After 10-11 days of incubation with lentivirus cultures were harvested and prepared for western blot using RIPA buffer (Thermo Fisher, 89900). Samples were heated for 10 minutes at 95° C with 50 mM DL-dithiothreitol (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: DNA probes were biotinylated at the 3’terminal using Biotin 11-dUTP (Thermo: R0081) and terminal deoxynucleotidyl transferase TdT enzyme (Thermo scientific: EP0161) in the presence of CaCl2 containing 5X reaction buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of 100 μM oligonucleotide bridge 2 and 2 units of T4 PNK (ThermoFisher) were added to 20 μl of the purified smDNA solution ...
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bioRxiv - Immunology 2021Quote: ... Protein secretion levels in culture supernatant were quantified via ELISA for human IgG (Invitrogen, 50-112-8849) and human BAFF (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... then TMB substrate (1-Step™ Ultra TMB-ELISA Substrate Solution, Thermo Fisher Scientific, Waltham, MA, USA) was added and developed for approximately 1 minute before stopping the reaction with 1 N HCl ...
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bioRxiv - Immunology 2020Quote: ... perfringens native ECP or native CWP were coated on ELISA plates (Nunc MaxisorpTM, ThermoFisher Scientific, Waltham, MA) at 10 μg/mL (IgA ...
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bioRxiv - Molecular Biology 2020Quote: ... Solid phase ELISA was performed by coating recombinant NEDP1 (20μg/well) on Maxisorb 96 well plates (Nunc) at 4°C overnight ...
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bioRxiv - Neuroscience 2021Quote: ... ISF Aβ42 in the 14 samples for each mouse was measured by ELISA (Invitrogen, KHB3441, 1:4). To determine Aβ42 half-life (Cirrito et al. ...
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bioRxiv - Immunology 2022Quote: Cell culture supernatants from BMDCs were analyzed by ELISA for IL-12p70 (R&D) and IFNβ (Invitrogen). Plates were read using a Tecan Sunrise microplate reader at 450 nm ...
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bioRxiv - Immunology 2021Quote: ... the assay was developed by addition of 1-Step Ultra TMB-ELISA solution (Thermo Scientific Cat# 34028) and reaction was stopped with 2 N sulfuric acid (Fisher Scientific) ...