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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... once with 1X SSC at room temperature for 15 min and twice with 0.5X SSC at room temperature for 15 min and finally with 1X PBS buffer containing 0.5 µg/mL 4′,6-diamidino-2-phenylindole (DAPI) and mounted onto ProLong Gold antifade reagent (Thermo Fisher Scientific) mounting media.
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bioRxiv - Cell Biology 2020Quote: ... with 106 viable cells/well in a 6-well plate precoated with matrigel in Williams E medium (Thermo Fisher # 12551-032) + 1x ITS (Thermo Fisher # 41400045 ...
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bioRxiv - Cell Biology 2020Quote: ... and Ctns-/- (n=6) mice were euthanized and the kidneys immediately removed and stored at −80°C in RNA Later (Life Technologies). Tissues were subsequently ground using Precellys 24 (Bertin Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Cas9-expressing hCMEC/D3 cells were each transduced separately by lentiviral particles expressing sgRNAs and selected by puromycin resistance at 10 μg/mL for 6 days (Life technologies). sgRNA sequences are listed in Table 2.
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bioRxiv - Cell Biology 2021Quote: ... were transiently transfected into 8 × 104 U2OS 2-6-3 cells in 4-well chamber slides using Lipofectamine 2000 reagent (Invitrogen, 11668019) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Embedded tissues were cut into 3-6 µm sections using a paraffin microtome (Leitz) and placed on SuperFrost® Plus slides (Menzel Gläser, Thermo Scientific). Deparaffination and antigen retrieval was done as described previously (Link et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 300 pmol of each siRNA and 6 μg of each DNA was electroporated within the dedicated tip (Thermo Fisher Scientific, MPK10096). Electroporation parameters consisted in 4 pulses of 10 ms width and a voltage of 1230 V ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μl of each binding reaction was loaded directly onto a pre-run 6% acrylamide DNA retardation gel (Thermo Fisher Scientific) and electrophoresed at ≤20 V cm−1 at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: Exosomes from mice plasma were harvested from age matched WT and SR-/- mice (6-8 weeks) plasma using the total exosome precipitation reagent for plasma (Invitrogen #4484451) as per instructions ...
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bioRxiv - Molecular Biology 2022Quote: βTC-6 cells (43) (ATCC, Manassas, VA) were cultured in low glucose DMEM (Dubelco’s Modified Eagle’s Medium) (Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Transfection of 2 µg pSpCas9-BB-2A-GFP-sgYBX1 was carried out with 6 µl of Lipofectamine 2000 (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... spheroids were transferred to an ultra-low attachment 6-well plate placed on an orbital shaker at 65 rpm and medium was changed to Neurobasal medium (Gibco ThermoFisher) supplemented with 2% B27 supplements (Gibco ThermoFisher) ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR was performed in three technical replicates for each sample using QuantStudio 6 Flex Real-Time PCR sequence detection system (Applied Biosystems) and FastStart Universal SYBR green Master mix (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
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bioRxiv - Genomics 2022Quote: ... 2.6 μL nuclease-free water and 5 μL cDNA template on the ABI Prism 7500 Fast Sequence Detection System (Life Technologies). Thermo-cycling conditions were as follows ...
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bioRxiv - Microbiology 2022Quote: ... from an overnight culture were washed with PBS and labeled with 8mg/ml sulfosuccinimidyl-6-[biotin-amido] hexanoate (Sulfo-NHS-LC-LC-Biotin, 21338, ThermoFisher Scientific) for 30 minutes at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg BA disks were prepared by transferring 10 μL of preheated 500 mg/mL BA in Dimethyl sulfoxide (DMSO) stock to blank antimicrobial disks (6 mm, Fisher Scientific). Single disks containing either 5 mg BA or 25 μg FLC (6 mm ...
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bioRxiv - Cell Biology 2022Quote: ... the cerebral cortices of 4 to 6 P0 rats were dissociated and chopped in Ca++/Mg++-free Hank’s Balanced Salt Solution (HBSS; Invitrogen, Carlsbad, CA). The tissue pieces were dissociated in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: We extracted total RNA from PFC and NAc tissues dissected from 6 rat brains using Trizol Reagent (Invitrogen, Cat, num. 15596018) and Zirconium Beads RNase Free (Next Advance ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR experiments were performed with SYBR Green Master Mix reagent with a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Relative quantifications were determined with the above primer pairs using delta delta threshold cycle methods with the following primers:
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bioRxiv - Cancer Biology 2022Quote: ... 250uL of strained sample (5% total marrow) was centrifuged at 600g for 6 minutes and resuspended in RNA Lysis/Binding Buffer (mirVana miRNA kit, Thermo Fisher) and stored at −80C ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:200 in blocking buffer for 30 min and mounted with ProLong Gold Antifade with DAPI (4′,6-diamidino-2- phenylindole; Thermo Fisher). Confocal laser micrographs were obtained with Zeiss LSM 880 laser microscope and analyzed with Zen black and Fiji software ...
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bioRxiv - Biophysics 2022Quote: 106 HEK cells were plated in 6-well plates and transfected with the indicated plasmids with Lipofectamine 2000™ reagent (Invitrogen). 24 hours after transfection ...
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bioRxiv - Biophysics 2022Quote: ... gels were functionalized with 0.5 mg/ml solution of sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH) (Thermo Fisher Scientific) prepared in 50 mM HEPES buffer (Santa Cruz Biotechnologies) ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T/17 cells (ATCC) were seeded at a density of 6×104/cm2 in Eagle’s Minimum Essential Medium (EMEM, Thermofisher, Waltham, MA), containing 10% FBS ...
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bioRxiv - Developmental Biology 2022Quote: Total protein extracts were obtained by homogenization of pools of 20 6-dpf larvae in ice cold RIPA buffer (ThermoFisher, 89900) and Complete EDTA-free protease inhibitor cocktail (Sigma ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and the second one carrying the mutation to be introduced prepared by PCR amplification of a 6 kb region using genomic DNA of evolved clones as template and high fidelity Phusion polymerase (ThermoFisher Scientific), were co-transformed into naturally competents cells of Ralstonia evolved clones ...
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bioRxiv - Cell Biology 2022Quote: ... 250.000 HEK293T cells were seeded per p35 plate (pre-coated Corning BioCoat Poly-D-Lysine 35 mm #354467 or uncoated Nunclon multidish 6 well plates, Thermo Scientific #140685 coated with 50 µg/ml Poly-D-Lysine solution (Gibco A38904-01 ...
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bioRxiv - Cell Biology 2022Quote: ... The transcripts of lysosome biogenesis genes and their transcription factors were amplified from the cDNA in QuantStudio 6 Flex real-time PCR system (Applied Biosystems). As a control 18S rRNA or β-Actin were amplified along with the lysosomal genes ...
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bioRxiv - Microbiology 2020Quote: ... cells were extensively washed and incubated with secondary antibodies in the presence of 4′,6-diamidino-2-phenylindole (DAPI, Molecular Probes) for 45 min at RT ...
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bioRxiv - Developmental Biology 2021Quote: ... transferred to 1.6 mL Eppendorf tubes and spun at 2000 rcf for 5 minutes at 4°C in an angle rotor (Thermo Scientific Fisher Heraeus Biofuge Primo R Centrifuge) ...
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bioRxiv - Developmental Biology 2021Quote: ... A target set of 20 short Alexa647 labelled oligo mouse Dux ViewRNA ISH probe (VB6-3223670-VC, type-6; ThermoFisher Scientific) was designed and used to hybridize to Dux RNA sequence ...
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bioRxiv - Microbiology 2020Quote: hiPSC-derived macrophages wild type or HPS4-deficient were plated on non-tissue culture treated 6-well plates (Thermo Fisher Scientific) and infected with S ...
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bioRxiv - Neuroscience 2021Quote: Non-neuronal cell cultures were prepared from postnatal day 0 (P0) C57BL/6 mouse cortices and cultured in Dulbecco’s MEM growth medium (Invitrogen Cat#11960044) supplemented with 10% FBS (Millipore Sigma Cat#F4135) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed and mounted in media (0.5% N propyl gallate, 70% glycerol, 1M tris pH8.0) containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Cat. No. D1306).
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bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
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bioRxiv - Neuroscience 2020Quote: ... All 27 samples from 3 groups and 6 GIS were divided and labeled using two sets of 5 mg 11 plex TMT reagents (Thermo Scientific A34808 ...
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bioRxiv - Bioengineering 2021Quote: Ovaries from 6 to 8 day-old CBA × C57BL/6 (F1) mice were collected and transferred to Leibovitz L-15 media (P/N 11415, Gibco, USA). The ovaries were dissected into 2–4 pieces and transferred into maintenance media (α-MEM ...
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bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
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bioRxiv - Microbiology 2022Quote: ... L2 fibroblasts were seeded at 1.5 x 106 cells per well in 6-well tissue culture-treated plates containing collagen-coated glass coverslips (Thermo Fisher Scientific) and inoculated with L ...
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bioRxiv - Molecular Biology 2022Quote: ... 500ng circRNAs per well for 24-well plates (2 μg for 6-well plates) were transfected with lipofectamine MessengerMax (Invitrogen, LMRNA003) on the next day when the cell culture must have >90% viability and be 70% confluent ...
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bioRxiv - Pathology 2022Quote: ... The iPSCs were continuously cultured and expanded on Geltrex coated 6-well plates with Essential 8 medium (ThermoFisher Scientific, Cat#A1517001). Human iPSC cell lines used in this manuscript ...
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bioRxiv - Cell Biology 2022Quote: ... 6) The enriched fraction was pre-cleared twice by adding 60 μL equilibrated DEPC-treated Protein A Dynabeads (ThermoFisher Scientific 10001D) and nutating at 4°C for 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... After extensive washing slides were air-dried and mounted in Gold Antifade containing 4’,6-diamidino-2-phenylindole (DAPI) (P3693; Life Technologies) for imaging ...
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bioRxiv - Immunology 2022Quote: ... and the wells were washed five times with wash buffer before the addition of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS, #37615, Thermo Fisher Scientific). Optical density was read 40 min later at 405 nm using a plate reader (SpectraMax i3 ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed in PBS-T and mounted under Prolong gold fluorescence media with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, P36931).
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bioRxiv - Neuroscience 2019Quote: ... The slices were incubated separately in wells with PBS and 5 ng/ml DAPI (4′,6-diamidino-2-phenylindole) for 20 min before they were rinsed in PBS and mounted in SlowFade Gold (Life Technologies). Superficial cortical areas of anterior and midcingulate cortex were imaged on a Leica SP8 inverted confocal microscope with a 63x oil immersion objective (numerical aperture = 1.4 ...
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bioRxiv - Microbiology 2019Quote: Cells were cultured in 6-well plate to 90% confluency and total RNA was extracted by Trizol (Life Technologies, Carlsbad, CA) and RT-qPCR was performed following the instruction of the manufacturer ...
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bioRxiv - Pathology 2019Quote: ... EB’s were returned to the ultra-low 6 well plate and transferred to Stage II media (DMEM, 15% KnockOut Serum Replacement (KOSR; Thermo Fisher), 1% NEAA ...
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bioRxiv - Neuroscience 2019Quote: ... Residual genomic DNA was removed from each of 6 biological replicates using the TURBO DNA-free™ Kit (Life Technologies, AM1907) per the manufacturer’s instructions ...