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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
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bioRxiv - Cell Biology 2019Quote: ... RNA was isolated from whole eyes of 6-month old cep290 mutant and sibling control animals (n = 9 per group) with Trizol (ThermoFisher 15596026). Reverse transcription using 1 μg RNA was performed with the iScript cDNA Synthesis kit (Bio-Rad 1708891) ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed in a final volume of 10 μL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... qPCR was performed in a final volume of 10 μL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Zoology 2020Quote: ... each immunoblot lane represents a cell lysate produced from an entire well of a 6-well cell-culture dish (Thermo Scientific, Nunc). Lyates were diluted with SDS loading dye (final concentration ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were seeded on a 6-well plate and transfected with 10 pmol of siControl (negative control #4390843, Ambion Silencer Select), siMCM2 (#s8536 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Alexa Fluor 488–conjugated phalloidin (#A12379) and 4’,6-diamidino-2-phenylindole (DAPI) (#1306) were obtained from Invitrogen (Carlsbad, CA, USA). IRDye800CW-labeled anti-rabbit secondary antibody (#926-32211 ...
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bioRxiv - Neuroscience 2020Quote: ... slides were washed and stained with 4′,6-Diamidin-2-phenylindol (DAPI, 5μg/ml) before mounting coverslips with ProlongTM Gold Antifade reagent (Thermo Fisher Scientific). Images were acquired using a LSM 710 confocal microscope (Zeiss ...
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bioRxiv - Physiology 2020Quote: ... The cells were transiently transfected for 16-24 hours with either human or mouse orthologs of TRPA1 using Fugene 6 transfection reagents and Opti-MEM (Thermofisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... hiPSCs at ~ 70% confluence (1.5×106 cells / well of a 6-well plate) were incubated in EDTA (Life Technologies #15575-020) for 4 min at room temperature (RT) ...
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bioRxiv - Microbiology 2020Quote: ... The synthesized coding DNAs with a 6-His-tag expressed at the C-terminus were cloned into the pPinkα-HC expression vector (Thermofisher, US) using XhoI/KpnI restriction sites ...
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bioRxiv - Microbiology 2021Quote: ... samples were unfrozen and aliquots of 6 ml per sample were fixed with Electron Microscopy Sciences 4% Paraformaldehyde (5025999, Fisher Scientific), stained with SYBR™ Gold Nucleic Acid Gel Stain (S11494 ...
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bioRxiv - Microbiology 2021Quote: ... in 6 well multi-dish Nunc UpCell Surface cell culture plates or 10 cm Nunc UpCell Surface cell culture dishes (Thermo Fisher) and differentiated to human monocyte-derived macrophages (hMDMs ...
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bioRxiv - Microbiology 2021Quote: Total RNA from virus infected (MOI=0.01) Vero E6 cells (106 cells/well, 6-well plate format) was extracted with TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Barcoded beads were resuspended in lysis buffer (200 mM Tris-HCl pH8.0, 20 mM EDTA, 6% Ficoll PM-400 (GE Healthcare/Fisher Scientific), 0.2% Sarkosyl (Sigma-Aldrich) ...
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bioRxiv - Genetics 2020Quote: ... followed by 35 cycles at 95 °C for 15 seconds and 30 seconds of 61.5 °C using a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher). Three technical replicates were performed for each cDNA sample ...
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bioRxiv - Immunology 2020Quote: ... qPCR was performed in a final volume of 10□μL per reaction in 384-well PCR plates using a thermocycler (QuantStudio 6 Flex, Applied Biosystems). Briefly ...
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bioRxiv - Neuroscience 2020Quote: ... 500 000 cells per well were seeded in a 6-well-plate in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco/Thermo Fisher, France) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... and 9.25 µL sterile water using the standard Power SYBR Green PCR Master Mix RT-PCR Protocol (Protocol Number 436721) on a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher). As positive controls for the WSP and WPCP primer sets ...
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bioRxiv - Immunology 2021Quote: ... The isolated nuclei were centrifuged at 500 x g for 5 min at 4°C and respectively resuspended with Nuclei wash buffer stained with 10 µg/ml 4′,6-diamidino-2-phenylindole (DAPI) before FACS (5mg/ml, Invitrogen D1306). After sorting using purity mode ...
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bioRxiv - Immunology 2021Quote: ... Cytokine concentrations in the day 6 supernatants of antigen-stimulated cultures were assessed by Luminex bead-based assay (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 5 x 105 HCT116 cells were seeded in a 6 well plate with Dulbecco’s modified Eagle medium (DMEM, Gibco®, Life Technologies) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Physiology 2019Quote: ... Gene expression analysis was then performed by quantitative PCR (qPCR) on a Quantstudio 6 Flex Real-time PCR System (Applied Biosystems) using the UBR5 mouse primers in Table 1 (also used in the other mouse bioengineered muscle analysis) ...
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bioRxiv - Physiology 2019Quote: Control and VDR-KD cells were plated at 1.0 × 1010 cells/well in 2 ml of growth medium in 6-well plates (Nunc, Roskilde, Denmark). Both myoblasts and myotubes were maintained and harvested as described previously ...
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bioRxiv - Cell Biology 2019Quote: ... was used to amplify two homology arms spanning exons 5 and 6 of PPP2R1A and cloned into a bacterial artificial chromosome (Life Technologies). The homology arms were cloned into vector pNX (Papi et al. ...
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bioRxiv - Genetics 2020Quote: ... Cell lines were either used within 6 months after purchase or were periodically authenticated by microsatellite fingerprinting (AmpFLSTR Identifiler, Thermo Fisher) by the Cancer Genomics Research Laboratory/NCI) ...
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bioRxiv - Immunology 2021Quote: ... and BMDCs were incubated at a ratio of 10 microspheres per cell for 6 h with 1.0-μm FluoSpheres® carboxylate-modified microspheres (ThermoFisher, F8851) labelled with a red fluorescent dye (580 nm excitation and 605 nm emission) ...
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bioRxiv - Neuroscience 2021Quote: ... Abi3-WT and -KO MØPs were maintained in 6-well plates with Roswell Park Memorial Institute 1640 (RPMI; Gibco®, ThermoFisher) supplemented with 10% heat-inactivated Foetal Bovine Serum (FBS ...
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Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: Transfection of sgRNA-Cas9 plasmids into Calu-6 cells for individual gene validation was done using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific), according to manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Each individual leaf tissue sample was processed in a plastic extraction bag using a HOMEX 6 homogenizer (BioReba, Swizerland) with 2.25 ml of TRIzol reagent following the manufacturer’s protocol (Thermo Fisher Scientific, USA). Concentration of the resulting RNA preparation was measured with a NanoDrop micro-volume spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2021Quote: ... cells were cultured on gelatin-coated 6-wells in 60% BRL conditioned medium to 70-90% confluency and transfected with Lipofectamin3000 (Invitrogen L3000008) according to the supplier protocol with 2 μg donor vector and 1 μg Cas9/guide vector ...
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bioRxiv - Biochemistry 2020Quote: ... 500 mM 6-aminohexanoic acid) was added to lysates right before loading samples into a NativePAGE Bis-Tris gel 4-16% (Life Technologies). The proteins were separated according to Wittig et al.32 and transferred onto a PVDF membrane using a standard Tris-glycine transfer buffer with 0.05% SDS ...
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bioRxiv - Biochemistry 2020Quote: ... Nuclei were stained with 10.9 μΜ 4’,6-diamidino-2-phenylindole (DAPI) and cover glasses were mounted in ProlongGold (Life Technologies). Cells were incubated 3x for 5 min in blocking solution to remove unbound antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... Culture were maintained in 6-,12- or 24-well plates in growth media consisting of 45% advanced DMEM/F12 solution (Gibco, # 126340010), 45% NBM solution (Miltenyibiotec ...
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bioRxiv - Bioengineering 2021Quote: ... hESCs were seeded at a density of 25×103 cell/cm2 on Matrigel-coated 6-well plates in Essential 8 medium (ThermoFisher, USA) on day −1 ...
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bioRxiv - Cancer Biology 2021Quote: ... and mPRs (PAQR5, 6, 7, 8, 9) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems). RT-qPCR plates with various cell-lines were prepared using an epMotion 5075 automated liquid handling system (Eppendorf) ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems). The following thermal cycling conditions were used ...
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bioRxiv - Cancer Biology 2021Quote: ... The fluorescent reaction was measured every 40 sec for 8 min and subsequent every 5 min for 6 times using a microplate reader (Thermo Scientific) with enzyme kinetics model ...
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bioRxiv - Immunology 2021Quote: The Fab fragments of Clone 2 and Clone 6 were generated from full length IgGs of Clone 2 and Clone 6 using a commercial PierceTM Fab Preparation Kit (Thermo Fisher). All procedures were performed following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The cells were transiently transfected with plasmids expressing GSPT1-HA wild type and variants (6 µg) using lipofectamine 3000 according to manufacturer’s protocol (Thermo Fisher Scientific). After 48 h ...
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bioRxiv - Microbiology 2021Quote: ... Culture dishes (15cm) coated with poly-L-lysine were plated with 6×106 neurons each and cultures maintained in Neurobasal media (Thermo Fisher) supplemented with GlutaMAX (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: Viral RNA was isolated from buccal swabs collected on dpi 6 and stored in 500 µl of TRIzol™ Reagent (Invitrogen). Total RNA extractions from buccal swab samples were performed using an adapted TRIzol™ manufacturers protocol with a 45 min precipitation step at −20° C ...
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bioRxiv - Neuroscience 2021Quote: Lateral ventricles containing the adjacent striatum was collected from 6 week WT and Nrmt1-/- mice and total RNA was lysed and extracted using TRIzol (Life Technologies) and chloroform ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were seeded at a density of 2.5 x 105 cells/well in a 6-well dish and transfected with CRISPR-Cas9 plasmids using the standard Lipofectamine 3000 (Thermo Fisher) protocol ...
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bioRxiv - Physiology 2021Quote: ... the libraries were quantified using a Collibri library quantification kit (Thermo Fischer Scientific, Ref. A38524100) in a QuantStudio 6 Flex (Life Technologies).
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bioRxiv - Pathology 2021Quote: ... qPCR analysis of gene expression was performed using QuantStudio 6 Real-Time PCR System and SYBR Green PCR Master Mix (Applied Biosystems). Analysis of relative gene expression was carried out using the comparative CT method (ΔCT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... ExpiFectamine CHO Enhancer (6 μl per ml of culture) and ExpiCHO Feed (240 μl per ml of culture) (Thermo Fisher Scientific) were added 18-22 hours post-transfection and the temperature was decreased to 33 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time quantitative PCR was performed using a QuantStudio 6 Flex real-time PCR system (Applied Biosystems, Foster City, California USA) following manufacturer’s protocols ...
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bioRxiv - Developmental Biology 2021Quote: ... Peptides were eluted along a 90 min gradient from 6 to 40% Buffer B (see StageTip purification) with the EASY□nLC 1,200 system (Thermo Fisher Scientific). Measurement was done on an Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... PC12 cells were plated in 6 well dishes and transfected 48h prior to lysis with 45 pmol siRNA using Lipofectamine RNAiMAX (Thermo Fisher); cells were lysed in RIPA buffer (50 mM Tris-HCl pH 7.4 ...