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Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Human embryonic kidney (HEK293T) and human neuroblastoma (SH-SY5Y) cells from ATCC were cultured in high glucose DMEM (Invitrogen) and DMEM-F12 (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA targeting human UBE3A or human MASTL (Integrated DNA Technologies) was transfected into cells using Lipofectamine RNAi MAX (Invitrogen). A non-targeting control siRNA was used as a control ...
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bioRxiv - Biophysics 2023Quote: N-terminally 6xHis-tagged human Gβ1 (S2-N340) and human Gγ2 (M1-C68) were synthesized and separately cloned into pFastBac1 (Thermo Fisher) and expressed as described above ...
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bioRxiv - Cell Biology 2023Quote: Human ApoE protein was quantified using the Invitrogen Human ApoE ELISA following the manufacturers guidelines (Invitrogen. Cat no. EHAPOE).
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bioRxiv - Molecular Biology 2023Quote: ... Hep-3B human hepatocellular carcinoma cells and MCF7 human mammary carcinoma cells were cultured in Eagle’s Minimum Essential Medium (EMEM) (Gibco) supplemented with 10% heat-inactivated and charcoal stripped fetal calf serum and antibiotics ...
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bioRxiv - Cancer Biology 2023Quote: ... Normal human melanocytes were grown in Melanocyte Growth Media 254 supplemented with Human Melanocyte Growth Supplement-2 (Life Technologies), calcium chloride (0.3 μM) ...
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bioRxiv - Cell Biology 2023Quote: Human umbilical vein endothelial cells (HUVECs) and human coronary artery smooth muscle cells (HCASMCs) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Molecular Biology 2023Quote: ... human breast cancer MCF7 cells and human bone osteosarcoma U2OS cells were in cultured in DMEM medium (Life Technologies) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2024Quote: ... corresponding to the concentration of the highest standard of recombinant human IFNγ (Human IFNγ Gamma Uncoated ELISA kit, Invitrogen). IFNγ concentrations below the level of detection by the ELISA standard curve were set to 0 pg/ml ...
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bioRxiv - Neuroscience 2024Quote: ELISAs for human Aβ40 and human Aβ42 were carried out using commercial kits (Aβ40: Invitrogen KHB3481; Aβ42: Invitrogen KHB3441) according to the manufacturer’s instructions ...
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Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... and (MtWOX5) 5’-CACCATGCAGACGGTCCGAGATCTGTC-3’ and 5’- CCTTCGCTTAAGTTTCATGTAA-3’ (AhWOX5) and cloned into pENTR-dTOPO (Invitrogen). The entry clones of AhWOX5 & MtWOX5 recombined through LR clonase Gateway technology (Invitrogen ...
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Interaction of the Xanthomonas effectors XopQ and XopX results in induction of rice immune responsesbioRxiv - Molecular Biology 2020Quote: ... The eight rice 14-3-3 genes cloned in the yeast two-hybrid vector pDEST22 (Invitrogen) were used from a previous study (Deb et al. ...
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bioRxiv - Cell Biology 2021Quote: ... N-hydroxysulfosuccinimide (sulfo-NHS) and 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride (EDC) from Thermo Scientific were dissolved in 18 MOhm DDW immediately before use ...
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bioRxiv - Bioengineering 2022Quote: ... 1-ethyl-3-(−3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was purchased from Fisher Scientific (Pittsburgh, PA, USA). 2-Bromo-2-methylpropionic acid (BMPA) ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were incubated for 24 minutes in a mixture of 3% 3-Aminopropyltriethoxysilane (ACROS Organics, 430941000) and 5% acetic acid in HPLC EtOH ...
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bioRxiv - Molecular Biology 2019Quote: ... the PCR products obtained with 2 primers (AV48_SMCHD1_gPCR_F: 5’-AGGAGCGCGTTTGAATCGG-3’, AV47_SMCHD1_gPCR_R 5’-CTTCGCGTACCTGACACACAC-3’) were TOPO-cloned (Thermo Fisher, #450071) and sent for sequencing.
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bioRxiv - Immunology 2020Quote: ... 3 μL RT reaction mix consisting of 3 μL of 50 μM random hexamers (Thermo Fisher), 0.8 μL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Molecular Biology 2022Quote: ... siM1-4: 5’ -ACTGGTAGCTTATTAAAGATT- 3’) and the HOXA1 siRNA (5’ - AGAACTTCAGTGCGCCTTATT- 3’) were purchased from Invitrogen™ (Silencer® Select siRNA ...
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bioRxiv - Cancer Biology 2024Quote: Total mRNA from 3 siCT and 3 siPAK2 independent experiments was extracted using TRIZOL reagent (Invitrogen) according to recommended procedures ...
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bioRxiv - Microbiology 2024Quote: ... This was followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for about 10 min ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated for 24 min in a mixture of 3% 3-aminopropyltriethoxysilane (ACROS Organics, 430941000) and 5% acetic acid in HPLC ethanol ...
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bioRxiv - Immunology 2023Quote: ... To assess active caspase 3 activity the CaspGLOW™ Fluorescein Active Caspase-3 Staining Kit (Invitrogen) was used ...
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bioRxiv - Cell Biology 2023Quote: ... Target mtDNA gene (Forward primer: 5′-CACCCAAGAACAGGGTTTGT-3′, Reverse primer: 5′-TGGCCATGGGTATGTTGTTAA-3′, Invitrogen custom primers) and reference 18S ribosomal RNA gene (Forward primer ...
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bioRxiv - Neuroscience 2024Quote: Rabbit monoclonal or polyclonal antibodies include those against 14-3-3 (Invitrogen, 51-0700, 1:1000); clathrin heavy chain (Abcam ...
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bioRxiv - Biophysics 2020Quote: MDA-MB-231 breast cancer cells were cultured in modified Eagle’s medium (MEM-F10, c.c.pro) supplemented with 10% fetal calf serum (FCS, Invitrogen) and 2.5 mM L-glutamin (c.c.pro ...
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bioRxiv - Biophysics 2020Quote: ... cell medium was replaced by 1 ml Leibovitz’s L-15 Medium (c.c.pro) containing 10% FCS and 25 nM Hoechst 33342 nucleic acid stain (Invitrogen) and incubated for 1 h at 37 °C before imaging.
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bioRxiv - Cancer Biology 2021Quote: ... After washing three times with MACS buffer consisting of PBS supplemented with 5 % FCS (Thermo Fisher Scientic) and 2 mM EDTA to remove unbounded cells ...
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bioRxiv - Immunology 2021Quote: ... and 10% fetal calf serum (FCS, Biowest) and minced into pieces of 1 mm3 in PBS (Gibco). The biopsies were enzymatically digested with 1 mg/mL collagenase IV (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... USA) supplemented with 10% fetal calf serum (FCS) and 1% penicillin-streptomycin solution (Invitrogen, Carlsbad, CA, USA) and were incubated at 37□ in a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were pre-incubated with anti-Fc receptor antibody (Thermal Fisher Scientific, Cat. No. 14-9161-73) at 1:20 ratio to block Fc receptor before staining ...
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bioRxiv - Immunology 2021Quote: ... and the absorbance (OD492nm) was measured using a Multiskan-FC microplate reader (Thermo Fisher Scientific, Helsinki, Finland). Ligation of the recombinant proteins was compared to the SUMO control.
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bioRxiv - Immunology 2019Quote: ... resuspended in William’s E medium containing 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific) and plated ...
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bioRxiv - Microbiology 2019Quote: ... in Dulbecco’s Modified Eagle medium (DME) supplemented with 1% v/v fetal calf serum (FCS) (Invitrogen, Australia) and 1% v/v Glutamax (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 % non-essential amino acids and sodium pyruvate and 30 % fetal calf serum (FCS) (Gibco, 10270-106). When cells reached confluency ...
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bioRxiv - Neuroscience 2020Quote: ... The absorbance at 450 nm wavelength was read on a microplate reader (Multiskan FC, Thermo Fisher Scientific). Mean values were calculated for triplicate sets of standards and samples ...
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bioRxiv - Biophysics 2020Quote: ... the substrate was washed with cell culture medium (DMEM w/glutamax, 10% FCS, 1% P/S; Gibco). 3T3 fibroblasts (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant Fc linked DR5 variants were biotinylated using EZ-Link Sulfo-NHS-SS-Biotin (Thermo Scientific 21331) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Absorbance was then measured at 595 nm using a Multiskan™ FC microplate photometer (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% FCS and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). Mammalian cells were incubated at 35°C in a humidified 5% CO2 incubator and insect cells at 28°C ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% FCS and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). Mammalian cells were incubated at 35°C in a humidified 5% CO2 incubator and insect cells at 28°C ...
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bioRxiv - Microbiology 2020Quote: ... the absorbance at 492 nm was measured with a microplate photometer (Multiskan FC, Thermo Fisher Scientific, USA).
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS) (Cytiva) and 2mM L-glutamine (Thermo Fisher Scientific) at 37°C in humidified 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... the enzymatic dissociation of the lamina propria was quenched by addition of 1ml of 100% FCS (ThermoFisher) and 80 μL of 0.5M EDTA and placed on ice for five minutes ...
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bioRxiv - Immunology 2022Quote: ... After another round of Fc blocking on ice for 15 min with sera from mouse (Thermo Fisher) and rat (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were recovered in Hanks’ balanced-salt solution (HBSS) supplemented with 1% fetal calf serum (FCS) (Gibco) or RPMI medium (Gibco ...
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bioRxiv - Microbiology 2021Quote: Monocytic cell line U937 (ATCC® CRL1593.2™) cultured in RPMI (Thermoscientific) supplemented with 10% FCS (Invitrogen) was used in all experiments ...
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bioRxiv - Cell Biology 2021Quote: ... Apical washes collected from ALI infections were thawed and serially diluted in DMEM with 1% FCS (Gibco), then added to cells and incubated for 2 hours before discarding and adding a 2.4% (w/v ...
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bioRxiv - Molecular Biology 2022Quote: ... were purchased from DSMZ (www.cell-lines.de) and cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) with 10% fetal calf serum (FCS, Gibco) and Glutamax (ThermoFisher Scientific) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and immediately measured the mixture’s absorbance at 575nm using a microplate reader (Thermo Scientific Multiskan FC, USA).
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bioRxiv - Microbiology 2019Quote: ... ModeK cells cultured in DMEM (DMEM Glutamax plus 10% FCS, non-essential amino acids and HEPES, Gibco) were transfected with 20 ng pNF-□B-Luc and 3 ng pRL-TK using FuGENE 6 (Roche) ...