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Citations for Thermo Fisher :
3851 - 3900 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Viral supernatant was collected at 48- and 72-hours post-transfection, concentrated by ultracentrifugation (1.5 h, 25,000 rpm, 4°C) and resuspended in PBS (Gibco, 10010023). The titer of the virus was determined as described previously (Rogers et al ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Flour 647-conjugated goat anti-rat IgG (H+L) antibody (1:2000) (Thermo Fisher Scientific, Waltham, MA, USA) and Alexa Flour 488-conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Bioengineering 2024Quote: ... After transfecting for 5 h the cells were spun down in a Sorvall ST 16R centrifuge (Thermo Fisher Scientific) at 50 x g for 4 min ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were incubated with secondary Donkey anti-Rabbit IgG (H+L) Alexa fluor plus 488 (1:500, Invitrogen, A32790) for 1 hour in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... and centrifuged at 42,000 rpm (∼157,000 g) for 17.5 h at 24 °C in a Sorvall wX+ Ultra Series ultracentrifuge (Thermo Scientific). The resulting DNA pellets with covalently bound proteins were washed with 70% ethanol and dissolved in 1× TE buffer (10 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... and cells were incubated at RT with 80 μL of coelenterazine h (2.5 μM final concentration) in Hanks’ balanced salt solution (HBSS) (Gibco) supplemented with 20 mM HEPES for 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was reverse transcribed to cDNA using the Maxima H Minus First Strand cDNA Synthesis Kit (ThermoFisher Scientific). qPCR was carried out using SYBR Select Master Mix (Applied Biosystems ...
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An improved TEAD dominant-negative protein inhibitor to study Hippo YAP1/TAZ-dependent transcriptionbioRxiv - Cell Biology 2024Quote: ... Secondary HRP-conjugated antibodies used were: Pierce peroxidase goat antimouse IGG (H + L) (ThermoFisher, catalog no. 31432; 1:2500), and Multi-rAb HRP-Goat Anti Rabbit Recombinant Secondary Antibody (H + L ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa 405-conjugated goat anti-mouse IgG (H+L) antibody (no. A-31553; Thermo Fisher Scientific; 1:250 dilution); Alexa 647-conjugated goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: Controls and siRNA to VAPA-treated BMM seeded onto glass coverslips and infected for 48 h were washed three times with cold Hank’s Balanced Salt Solution (HBSS, Invitrogen) and incubated in DMEM (without FBS ...
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bioRxiv - Cell Biology 2024Quote: ... GST-PLK-2 was expressed for 48 h in Sf9 cells using the Bac-to-Bac system (Thermo Fisher) and purified over Glutathione Sepharose (Cytiva ...
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bioRxiv - Biophysics 2020Quote: ... MCF 10A cells (ATCC) were cultured in Dulbecco’s modified Eagle medium (D-MEM) 1g/l glucose (Invitrogen), supplemented with 10% horse serum (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... and 40 µg/mL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Thermo Scientific). Plates were incubated at room temperature for 48-72 h ...
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bioRxiv - Immunology 2020Quote: ... Cells were cultured in complete DMEM (4.5 g/L D-Glucose, L-Glutamine, 1% penicillin/streptomycin, Gibco) supplemented with 10% FBS (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... as directed by the manufacturer and employing oligo d(T)16 (Thermo Fisher Scientific; Cat. No. N8080128), dNTP Mix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: Cell culture supernatants from BMDCs were analyzed by ELISA for IL-12p70 (R&D) and IFNβ (Invitrogen). Plates were read using a Tecan Sunrise microplate reader at 450 nm ...
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bioRxiv - Microbiology 2021Quote: ... in complete neural stem cell (CNSC) medium containing Knock-out D-MEM/F-12 (A1370801, Thermo Fisher), 2 mM Glutamax (35050038 ...
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bioRxiv - Cell Biology 2021Quote: ... and 100 μM D-luciferin) for 1 hour then cycloheximide (CHX) (Fisher Scientific, Hampton, NH, cat # 50255724) was added at a final concentration of 100μg/ml diluted in serum free media.
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bioRxiv - Neuroscience 2020Quote: ... ear biopsies (one mm in diameter) were digested in Dulbecco’s Modified Eagle’s Medium (DMEM, HyClone) containing 2.5 mg/ml collagenase D (Gibco) and 1.25 mg/ml pronase (Millipore ...
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bioRxiv - Bioengineering 2021Quote: The silanized thin-glass sheet was coated with poly-D-lysine (PDL) (100 μg/mL) (Gibco, USA) and laminin (50 μg/mL ...
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bioRxiv - Bioengineering 2020Quote: ... with 2.5 ng/mL recombinant human fibroblast growth factor R&D Systems) and fusion medium (low-serum medium - 94 % Dulbecco’s Modified Eagle’s Medium, Gibco, 5 % horse serum ...
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bioRxiv - Biochemistry 2021Quote: ... Antibody-bound complexes were immunoprecipitated with Novex DYNAL Dynabeads Protein G (Invitrogen, cat. no. 10-003-D) or protein A/G agarose beads (EMD Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Cultured cells were maintained in Dulbecco’s Modified Eagle Medium (D-MEM) (11995-065, GIBCO Life Technologies, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... Cultured cells were maintained in Dulbecco’s Modified Eagle Medium (D-MEM) (11995-065, GIBCO Life Technologies, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... mouse anti-Dsg1 (P124 - PROGEN cat# 651111, RRID:AB_1541107; R&D Systems cat# MAB944, RRID:AB_2093296; 27B2 – Thermo Fisher Scientific cat# 32-6000 ...
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bioRxiv - Cell Biology 2021Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) (cat#D-1306) and TRIzolTM (cat#15596018) were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Plant Biology 2020Quote: ... from cDNA using the primers indicated in Supplemental Table 3 and transferred into pENTR-D-TOPO (Invitrogen) via Gibson assembly using NEB Gibson Assembly Master Mix according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were trypsinized and resuspended in HBSS containing 20 uM H2DCFDA (Thermo Fisher Scientific, Cat# D-399). Cells were incubated in a 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Pelleted cells were resuspended in 1x D-PBS and viability staining with Fixable Live/Dead Aqua (Invitrogen) was performed for 30 min at RT (protected from light) ...
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bioRxiv - Microbiology 2020Quote: ... cell lines were washed thrice using Dulbecco’s phosphate buffered saline (D-PBS) without calcium and magnesium (Gibco) and harvested with TrypLE™ express enzyme (1X ...
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bioRxiv - Neuroscience 2020Quote: ... Protein expression was induced by the addition of 1 mM isopropyl β-D-thiogalactoside (IPTG) (Thermo Scientific) for 16 h at 22°C ...
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bioRxiv - Plant Biology 2020Quote: ... A 2.1 kb sequence containing the NPF7.3 promoter was amplified from Arabidopsis genomic DNA by PCR using primers NPF7.3pro-topo-F and NPF7.3pro-R and cloned into pENTR/D-TOPO (pENTR-NPF7.3pro) (Invitrogen). The NPF7.3 promoter sequence was amplified again by PCR from the pENTR-NPF7.3pro construct using primer pairs NPF7.3pro-attL1-IF-F/NPF7.3pro-NPF7.3CDS-IF-R ...
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bioRxiv - Immunology 2022Quote: ... total RNA was prepared using lysing matrix D (MP Biomedical) containing 1 mL of TRIzol reagent (ThermoFisher) and homogenization at 30 s at 6.0 m/s twice using MP Biomedical Fastprep 24 Tissue Homogenizer ...
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bioRxiv - Microbiology 2022Quote: Recombinant human CD81 protein (R &D Systems) was coated (4µg/ml) on ELISA plate (Thermo Fisher Scientific) and incubated overnight at 40C ...
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bioRxiv - Cancer Biology 2021Quote: ... The p75/LEDGF isoform was cloned in pENTR/D-TOPO cloning vector (Invitrogen, Cat. No. 45-0218), with PCR-based techniques ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized using a mix of random hexamers and oligo d(T) by Superscript IV (Invitrogen). Technical triplicates qRT-PCR were performed with SYBR-green I Master Mix (Roche ...
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bioRxiv - Cell Biology 2021Quote: Cells were plated on poly-D-lysine−coated borosilicate glass Lab-Tek eight-well chambers (Thermo Scientific). For microtubule immunofluorescence labeling ...
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bioRxiv - Immunology 2021Quote: ... we first coated 6-well plates with 0.5 μg/mL poly-D-lysine (ThermoFisher, Cat. No. A3890401) for 1 to 2 hours at room temperature (RT) ...
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bioRxiv - Neuroscience 2020Quote: ... and combined with 50μl 1× D-Luciferin Working Solution supplemented with 1× Firefly Signal Enhancer (Thermo Scientific Pierce Firefly Signal Enhancer ...
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bioRxiv - Neuroscience 2020Quote: PFA-fixed brains were separated into hemispheres and small DiI crystals (Cat. no. D-3911, ThermoFisher Scientific) were placed in the exposed thalamic surface ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were plated on poly-D-lysine/laminin coated dishes with the following medium: Neurobasal-A (Gibco) completed with L-glutamine (Lonza ...
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bioRxiv - Neuroscience 2021Quote: ... Cultured cells were maintained in Dulbecco’s Modified Eagle Medium (D-MEM) (11995-065, GIBCO life technologies, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: ... Tissues were incubated with primary antibodies (mouse anti-HuC/D IgG clone 16A11 at 1:200 [Thermofisher] ...
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bioRxiv - Neuroscience 2020Quote: ... Transfected HEK293 cells were solubilized in 0.1-1% (w/v) n-dodecyl-β-D-maltoside (ThermoFisher Scientific), 1 mM phenylmethylsulfonyl fluoride (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: Macrophages grown in T75 flasks for 14 d were washed once with sterile Dulbecco’s PBS (Life Technologies) and treated with accutase (Biolegend ...
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bioRxiv - Biophysics 2022Quote: ... Mountant for STED imaging (Figure 3A, C, D; Figure S2A) was Prolong™ Diamond Antifade (Invitrogen. P36965). Mountant for confocal imaging (Figure 1H ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were then stimulated with 20 µl of CD3/CD28 Dynabeads (Thermo Fisher Scientific, 11131-D) per million cells ...
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bioRxiv - Developmental Biology 2020Quote: ... Poly-D-Lysine and N-Cadherin coated coverslips and cultured in plating media (Neurobasal (Gibco, #21103-049) medium supplemented with 10% heat-inactivated FBS and 1X penicillin/streptomycin/glutamine).
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bioRxiv - Immunology 2020Quote: ... containing a 5′-CACC-3′ sequence required for directional TOPO cloning in pENTR/D-TOPO (Invitrogen, USA), and a reverse primer [“Tgd057 R HA stop” 5’-TTACGCGTAGTCCGGGACGTCGTACGGGTACTCGACCTCAATGTTGTATTC-3’] ...
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bioRxiv - Developmental Biology 2020Quote: ... The cen-bcd-3’UTR fusion was assembled and directionally cloned into the pENTR-D vector (Invitrogen) by Gibson assembly using 5-fold molar excess of the bcd-3’UTR ...