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Citations for Thermo Fisher :
4101 - 4150 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and rabbit anti-mouse IgG (H+L) cross-adsorbed secondary antibody (Alexa Fluor® 488 (ThermoFisher Scientific, cat# A-11059)) were used at a 1:500 dilution as secondary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was generated by Reverse Transcription mix containing the Maxima H Minus reverse transcriptase (Thermo Fisher Scientific, Waltham, MA, USA), oligo-dT primer E3V6NEXT and template switch primer E5V6NEXT ...
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bioRxiv - Molecular Biology 2022Quote: ... the cell cycle was analyzed by measuring BrdU incorporation into cell DNA 18 h after intraperitoneal injection of 200 mg BrdU (Invitrogen). Cells were labeled following the protocol for the BrdU Staining Kit for flow cytometry (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated for 1 h at RT with Cy5-conjugated goat anti-rabbit antibody (1:250, #A10523, Thermo Fisher Scientific) diluted in blocking buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Complementary DNA (cDNA) was synthesized from RNA using the Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher Scientific). Real-time qPCR was performed using iTaqTM Universal SYBR® Green Supermix (Bio-rad ...
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bioRxiv - Cell Biology 2022Quote: ... slices were labelled with SPY650-DNA (1:1000, #SC501, Spirochrome) for 1 h and CellTraceTM CFSE (1:1000, #C34554, ThermoFisher) for 30 min ...
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bioRxiv - Cell Biology 2022Quote: hTERT RPE-1 cells were transfected with 20 nM (final concentration) of the respective siRNA for 48 h using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... First-strand cDNA was synthesized by RT-PCR using a RevertAIT H Minus First Strand cDNA Synthesis kit (Thermo Scientific). qPCR was performed using the Mx3005P system (Stratagene ...
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bioRxiv - Cell Biology 2022Quote: ... The primary antibody was added at a ratio of 1:100 and incubated for at least 1 h at room temperature while the secondary antibody (1:300 Invitrogen) was allowed to incubate with the sample for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... cells were transfected 24 h after seeding with 50-100 nM SMARTpool siRNAs (Dharmacon) for gene silencing and Lipofectamine 2000 (1000186, Invitrogen), with two rounds of knockdown for 5 days ...
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bioRxiv - Neuroscience 2020Quote: ... the peptides from each sample were reacted for 1 h with the a ppropriate TMT-NHS isobaric reagent (Thermo Fisher) in 40% (v/v ...
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bioRxiv - Immunology 2020Quote: ... cells were restimulated with PMA (50 ng/ml) and ionomycin (500 ng/ml) for 4 h in RPMI 1640 (Invitrogen) containing 8% FCS ...
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bioRxiv - Microbiology 2021Quote: ... primary antibody for 16 h at 4 °C and detected with anti-rabbit-Alexa488 (1:200, A11008, Invitrogen, CA, USA) secondary antibodies for 40 min at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... diluted cell lysates were incubated for 16 h at 4°C with Dynabeads Protein G (100-O3D, Thermo Fisher Scientific) precoated with 5 μL of anti-MYC antibody (ab32 ...
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bioRxiv - Developmental Biology 2021Quote: ... and goat anti-rabbit IgG (H+L) conjugated with Alexa Fluor 488 was from Molecular Probes (A11008, 1/400 dilution). Positive cells were counted from blinded images using the Cell Counter tool of Fiji.
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bioRxiv - Molecular Biology 2020Quote: ... sections were then incubated for 2 h in blocking/permeabilization buffer with goat anti-mouse AlexaFluor 568 (Invitrogen, Carlsbad, CA) at 1:500 dilution ...
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bioRxiv - Molecular Biology 2020Quote: ... in blocking solution for 1 h at room temperature followed by 3x PBS rinses and incubating with Hoechst 33342 (1µg/mL Molecular Probes) and anti-rabbit AlexaFluor 546 or 568 (1:1000 Molecular Probes ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized from total RNA using poly-Thymin and random nonamer primers and Superscript III RNase H reverse transcriptase (Invitrogen). Quantitative real time PCR was carried out using the GoTaq qPCR Master Mix (Promega ...
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bioRxiv - Microbiology 2020Quote: ... in TBST buffer with 0.5% BSA and goat α-mouse IgG (H+L) HRP conjugated secondary antibody (1:10,000, Invitrogen A28177) were used to detect His-tagged proteins on the blot ...
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bioRxiv - Microbiology 2020Quote: ... DMEM 10 % FBS for at least 48 h then 150 000 cells were reverse transfected with 500 ng of GFP using lipofectamine 2000 (Invitrogen) in 24 well plate.
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bioRxiv - Microbiology 2021Quote: ... and HepG2 cells were collected 46 h after infection of CSPwt or CSPmut sporozoites and lysed by 1 mL Trizol (Invitrogen). 100μL liver homogenate or 1 mL of cell lysis were used for total RNA extraction using Trizol in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... all wells were processed for 1 h with appropriate fluorescent secondary antibodies (Alexa Fluor 594 donkey anti-rabbit (1: 1,500, Life Technologies); Alexa Fluor 488 donkey anti-chicken (1:500 ...
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bioRxiv - Immunology 2020Quote: ... A second incubation was performed for 1 h at room temperature with anti-mouse Alexa Fluor 568-conjugated (Thermo Scientific) 1:200 in 1% PBSA and then incubated for 5 min with Syto9 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific). Pre-cleared supernatants were incubated overnight at 4°C with 1:1250 dilution of Rabbit (DAIE ...
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bioRxiv - Microbiology 2021Quote: ... They were induced to sporulate for 24 h and the samples were prepared as previously described (25) using magnetic Dynabead Protein G (Invitrogen) coupled to anti-FLAG M2 antibody (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2020Quote: ... HRP-conjugated anti-mouse were incubated for 1 h at room temperature and visualized by enhanced chemiluminescence (ECL, Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated at room temperature for 1 h with the polyclonal specific anti-oligomer A11 antibody (Life Technologies) or the fibril-specific monoclonal antibody OC (Millipore) ...
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bioRxiv - Neuroscience 2020Quote: ... coverslips were washed three times in PB 0,12 M and incubated with a goat anti-rabbit IgG (H+L) highly cross-adsorbed AlexaFluor A594 (1:1.000, Life Technologies) antibody diluted in the blocking solution for 2H at RT ...
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bioRxiv - Neuroscience 2020Quote: ... slices were washed three times in PB 0,12 M and incubated with a goat anti-rabbit IgG (H+L) highly cross-adsorbed AlexaFluor A594 (1:1.000, Life Technologies) diluted in blocking solution for 2H ...
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A chlorzoxazone-baclofen combination improves cerebellar impairment in spinocerebellar ataxia type 1bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies used were as follows: Alexa Fluor 488 goat anti-mouse IgG (H+L) (1:200; Invitrogen, Cat. #A11001), Alexa Fluor 488 goat anti-rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2022Quote: ... The first strand cDNA for the expressed H and L chain was synthesized using a commercially available Superscript III reverse transcription kit (Invitrogen). The 25 μL reverse transcription reaction mixture consisted of 1X PCR buffer ...
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bioRxiv - Cell Biology 2022Quote: ... followed by washing with Tween-20/PBS and 1 h incubation with HRP-conjugated goat anti-mouse IgG at 1:40 000 (Cat# A16066, Invitrogen) in 100 µl per well of Tween-20/PBS ...
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bioRxiv - Microbiology 2022Quote: ... and 0.2% Triton X-100 at RT for 1 h, and then blocked with an anti-dsRNA antibody (Scicons, 10010200) and a fluorescent secondary antibody (Invitrogen). Images were captured with the CellInsight CX7 High-Content Screening (HCS ...
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bioRxiv - Physiology 2022Quote: ... Cells were then incubated overnight at 4°C with antibodies against claudin-4 diluted 1:500 in 0.2% BSA followed by a 1 h incubation with Alexa Fluor 488-conjugated goat anti-rabbit (cat. no. A-11017; Invitrogen) diluted 1:500 and were finally mounted on microscope slides using Vectashield mounting medium (Maravai Life Science ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated with Cy5-pDNA/PP-sNp or naked Cy5-pDNA for 4 h in Opti-MEM I Reduced Serum Medium (31985062, Invitrogen). The cells were further collected for the analysis of mean fluorescence intensity (MFI ...
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bioRxiv - Developmental Biology 2022Quote: ... 40 whole embryos at stage 3 (18 h AEL) were homogenized using disposable pellet pestles (12-141-368; Fisher Scientific) and 1.5 mL Eppendorf tubes (0030125150 ...
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bioRxiv - Cancer Biology 2022Quote: ... Brightfield hematoxylin and eosin (H&E) high-resolution images were taken using the EVOS FL Auto Imaging System (ThermoFisher Scientific) using a 10X objective so as to differentiate tumor and non-tumor regions ...
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bioRxiv - Bioengineering 2022Quote: ... Alexa Fluor 488 goat anti mouse IgG (H+ highly cross adsorbed secondary anti body, Hoechst 3334333422 (1(1 mg/mL)mg/mL) were purchased from ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and then for 1 h with a 1/10 000 dilution of anti-mouse– horseradish peroxidase conjugate (Thermo Fisher Scientific). His-tagged proteins bound to the membrane by virtue of its interaction with lipid ...
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bioRxiv - Immunology 2020Quote: ... for 2 h at 37°C and 5% CO2 in the presence of dextran-rhodamine (0.5 mg/mL) (Molecular Probes). mAb-untreated Pf sporozoites were used to measure the maximum traversal capacity ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were washed in PBS before incubation with mouse anti-BrdU (B44, Becton Dickinson 347580, 1:200) for 1 h and subsequently with antimouse IgG AlexaFluor 488 (ThermoFisher) for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by 1 h incubation with 1:1000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark ...
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bioRxiv - Neuroscience 2021Quote: ... We used the following secondary antibodies conjugated with Alexa Fluor fluorochromes: 488 goat anti-guinea-pig IgG H+L (catalog #A11073, Invitrogen/ThermoFisher), 555 donkey anti-rabbit IgGs H+L (#A-31572) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h in 2% donkey serum solution containing secondary antibodies conjugated to anti-rabbit Alexa488 (Invitrogen, Massachusetts ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed again in PBS and incubated for 2 h at room temperature in secondary antibody in carrier solution (donkey anti-goat 647, 1:1000, ThermoFisher; donkey anti-rabbit 405 ...
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bioRxiv - Molecular Biology 2021Quote: ... of THP-1 monocytes +/− ML-60218 exposure and THP-1 macrophages (72 h PMA) using the mirVana miRNA isolation kit according to instructions (AM1560, Invitrogen). Nuclear and cytoplasmic THP-1 fractions were isolated using the NE-PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: Cells were transfected with 40-50 nM of siRNAs at the time of plating and again 24 h later with either Lipofecatamine RNAiMax or Lipofectamine 2000 (Invitrogen) using manufacturer’s protocols.
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bioRxiv - Cancer Biology 2020Quote: ... 300,000 cells in 6-well plate were transfected for 48 h with 2 µg of plasmid DNA using Lipofectamine 2 000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... The cells were left to fix for 1 h at room temperature before transferring 5 μl into 15 μl ProLongTM Gold Antifade mountant (ThermoFisher). For cell membrane staining ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS twice and incubated for at least 1 h with 20 µg/ml streptavidin (Thermo Fisher) in 1 % BSA/PBS at room temperature ...