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Citations for Thermo Fisher :
3651 - 3700 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 1 μg of linearised plasmid was incubated for 2 h at 37°C with 1U T7 RNA polymerase (ThermoFisher), 5 mM DTT ...
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bioRxiv - Microbiology 2020Quote: ... followed by 1 h incubation with goat anti-mouse AlexaFluor488 (A21131, Invitrogen; 1:1000 in PBS with 1% BSA). Cell nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole ...
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bioRxiv - Microbiology 2020Quote: ... Alexa Fluor 488 goat anti-rabbit IgG (H + L) secondary antibody and Hoechst 33342 were purchased from Molecular Probes. Paraformaldehyde (PFA ...
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bioRxiv - Microbiology 2021Quote: ... RNA was treated 1 h at 37 °C with 2 units of Turbo DNAse (ThermoFisher Scientific, Waltham, MA, USA), purified using NucleoSpin RNA Clean-up (Macherey-Nagel ...
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bioRxiv - Microbiology 2021Quote: ... followed by 2 h staining with secondary donkey anti-mouse Alexa Fluor 546 antibody (Invitrogen, A10036, 1:500 dilution) and 1μg/ml DAPI.
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bioRxiv - Molecular Biology 2021Quote: ... 48 h later cells were collected and RNA isolated with a Purelink RNA mini kit (ThermoFisher Sci., Rockford, IL) for RNA-Seq analysis as described32 ...
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bioRxiv - Immunology 2020Quote: Recovered beads were transferred to a 1.5 mL tube and reverse transcription was performed using Maxima H-reverse transcriptase (Invitrogen). Beads were then treated with ExoI to digest unoccupied primer sites (NEB).
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bioRxiv - Immunology 2021Quote: ... The staining was revealed using IgG (H&L) specific secondary antibody HRP-conjugated (1:500, Novex by Life Technologies) and DAB (3,3’-Diaminobenzidine ...
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bioRxiv - Immunology 2021Quote: ... and beads were resuspended in a master mix for reverse transcription containing Maxima H Minus Reverse Transcriptase (ThermoFisher EP0753) and buffer ...
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bioRxiv - Neuroscience 2021Quote: ... Goat anti-rabbit IgG (H+L) Cross-adsorbed secondary antibody with Alexa Fluor 555 (Invitrogen, cat no. A-21428), DAPI (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... A secondary antibody (donkey anti-mouse coupled with Alexia 488 Ig G [H+L]; Life Technologies, Thermo Fisher Scientific) diluted at 1:300 was incubated for 2 h on slides ...
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bioRxiv - Cell Biology 2021Quote: ... A secondary antibody (donkey anti-mouse coupled with Alexia 488 Ig G [H+L]; Life Technologies, Thermo Fisher Scientific) diluted at 1:300 was incubated for 2 h on slides ...
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bioRxiv - Cell Biology 2021Quote: ... samples were washed 3X with PBST at RT for 1 h and mounted with SlowFade Gold antifade reagent (ThermoFisher). Samples were analyzed using a Nikon inverted TE2000-E microscope equipped with phase contrast and epifluorescence ...
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bioRxiv - Genetics 2020Quote: ... and subsequently converted to cDNA using the RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo scientific, catalogue K1631). qPCR was done using the Syber green mix (Bio-Rad ...
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Rac1, Rac3 GTPases and TPC2 are required for axonal outgrowth and migration of cortical interneuronsbioRxiv - Neuroscience 2022Quote: ... Electroporated slices were placed for 1 h in MEM medium (10% FBS, 0.5% glucose, 1% Pen/Strep, in MEM with glutamine, all Gibco) in a 5% CO2 humidified incubator at 37 °C and then incubated in Neurobasal/B27 medium for 18-24 h followed by the time lapse imaging (Denaxa et al. ...
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bioRxiv - Neuroscience 2022Quote: ... Alexa Fluor 546-conjugated goat polyclonal anti-mouse IgG (H+L) (1:500; A11030, Molecular Probes, Eugene, OR, USA); Alexa Fluor 546-conjugated goat polyclonal anti-rat IgG (H+L ...
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bioRxiv - Molecular Biology 2022Quote: ... and the goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (Alexa Fluor™ 546) (Invitrogen, A-11010) were used in a 1:1000 dilution in blocking buffer ...
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bioRxiv - Microbiology 2022Quote: ... Transcription reactions were incubated 4 h at 37 °C and transcripts were purified with TRIzol Reagent (Thermo Fisher Scientific) and Direct-zol RNA Miniprep kit (Zymo Research) ...
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bioRxiv - Cancer Biology 2022Quote: ... The RNA obtained was reverse-transcribed to cDNA using the RevertAid H minus First Strand cDNA Kit (Life Technologies) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were first pre-incubated for 1 h in glucose-free RPMI 1640 GlutaMAX-I medium (Life Technologies, Inc.), followed by incubation with Krebs-Ringer buffer (KRB ...
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bioRxiv - Biophysics 2022Quote: ... Secondary antibodies [Alexa 647-goat anti-mouse or Alexa 647 Goat anti-Human IgG (H+L) Cross-Adsorbed (Invitrogen)] at 1:1000 dilution were then incubated for 60 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... antibodies in PBS containing 1% BSA for 1 h and then with Alexa Fluor secondary antibodies (1:1000, Invitrogen) and Hoechst 33258 (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA was transcribed to cDNA using the RevertAid H Minus First Strand cDNA Synthesis Kit (#K1631, Thermo Fisher Scientific) according to manufacturer’s instructions.
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Purkinje cardiomyocytes of the ventricular conduction system are highly diploid but not regenerativebioRxiv - Developmental Biology 2022Quote: ... goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody AlexaFluor 647 conjugate 1:500 (A21244, Thermo Fisher Scientific). Intestines were removed freshly and formed into Swiss rolls [18] and fixed overnight in 10% formalin ...
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bioRxiv - Genetics 2022Quote: ... Sheep IgG (H+L) Highly Cross-Adsorbed Donkey anti-Ovine HRP (1:2000-1:6000, ThermoFisher Scientific, catalogue #A16047)
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bioRxiv - Molecular Biology 2022Quote: ... The purified RNA was used to synthesize cDNA following the RevertAid H Minus First Strand cDNA Synthesis Kit (ThermoFisher). The reaction mixture was incubated at 42°C for 1 h and then terminated by heating at 70°C for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... cDNA synthesis was performed by using Maxima H Minus First Strand cDNA Synthesis Kit (ThermoFisher Scientific, Waltham, MA, USA). Oligo (dT)18 and random hexamer primers were used in the reaction mixture according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 488 (Thermo Fisher Scientific, A-21206), and donkey anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... and donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 594 (Thermo Fisher Scientific, A21207).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... adult WT C57BL/6J mouse testes were dissected and incubated at 37 °C for 6 h in either Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12, Thermo Scientific) or compound 5 (100 μM ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthetized by reverse transcribing 1 μg of total RNA using RevertAid H minus reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Ambion) was reverse-transcribed using Maxima H Minus cDNA Synthesis Master Mix with dsDNase kit (M1682; Thermo Fisher Scientific), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: Cultured viruses were collected by centrifugation at 9,000 rpm for 1 h at 4°C (Sorvall ST8FR, Thermo Scientific) and resuspended in phosphate-buffered saline ...
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bioRxiv - Plant Biology 2022Quote: ... then incubated in 0.2x SSC for 2 h at 55°C and treated with RNase A (Thermo Scientific #EN0531) at 37°C for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... For haematoxylin and eosin (H&E) staining conventional methods on a Gemini AS Automated Slide Stainer (Thermo Fisher Scientific) were used ...
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bioRxiv - Neuroscience 2022Quote: ... and Alexa Fluor 568 donkey anti-rabbit IgG (H&L) highly cross-adsorbed secondary antibody (Thermo Fisher Scientific, A10042) were used in 1:1,000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... Cultures were washed and incubated for 3 h with appropriate secondary antibodies (1:1000, in PBS with 10 % NGS or NHS, 0.1 % Triton X-100; Invitrogen). TO-PRO® or DAPI (1:5000 in PBS for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... First-strand cDNA synthesis was performed with the RevertAid H Minus First Strand cDNA Synthesis kit (Thermo Fisher Scientific), following manufacturer’s instructions ...
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ATM-Mediated Double-Strand Break Repair Is Required for Meiotic Genome Stability at High TemperaturebioRxiv - Plant Biology 2022Quote: ... The secondary antibodies goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor 555 (Invitrogen, A-32732), goat anti-rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2022Quote: ... and by HRP-conjugated goat anti-rabbit IgG (H+L) secondary antibody (# 31460, Thermo Fisher Scientific, Prague, Czech Republic)
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bioRxiv - Synthetic Biology 2024Quote: ... Incubation with Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor™ 647 (Invitrogen, A-21244) in 1:500 dilution was also performed in a blocking buffer for 2 hours at room temperature ...
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bioRxiv - Physiology 2024Quote: ... secondary antibodies were applied including Alexa flour 647 donkey anti-rabbit IgG (H+L) (1:200, A31573, Life technologies) and in 5 % of skim milk in PBS-T for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... a 2 h drug treatment was carried out in the presence of 1µM LysoTracker Deep Red (Invitrogen, Cat. # L12492). Lysosensor Green DND-189 was added to the complete medium at a concentration of 1 µM and the cells were incubated at 37 °C for 2 h ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were successively incubated with indicated primary antibodies in PBS/BSA 1% saponin 0.1% for 2 h at RT and with Alexa Fluor-conjugated secondary antibodies in PBS/BSA 1% saponin 0.1% for 1 h raised against mouse or rabbit immunoglobulins (Invitrogen). After washes ...
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bioRxiv - Neuroscience 2023Quote: ... and Goat anti-Mouse IgG (H+L) Alexa Fluor 594-conjugated secondary Ab (1:200; Invitrogen, Cat# A11005, RRID:AB_2534073) were used ...
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bioRxiv - Neuroscience 2023Quote: ... and Goat anti-Rabbit IgG (H+L) Alexa Fluor 488-conjugated secondary Ab (1:200; Invitrogen, Cat# A11034, RRID:AB_2576217) were used ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was removed after 24 h and cells were cultured in fibroblast medium with 10% FBS (Gibco, 16000-044) in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Microbiology 2024Quote: ... Cultures were filtered 16 h later using 0.45 and 0.22 µm syringe filters with a polyethersulfone (PES) membrane (ThermoFisher Scientific), and the flow through was tittered by plaque assay as described below ...
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bioRxiv - Neuroscience 2023Quote: ... The mAbs were detected using Alexa Fluor-594 conjugated goat anti-mouse IgG (H+L) (Invitrogen Cat# A-11032) and the reference Ab with an Alexa Fluor-488 conjugated goat Ab against the appropriate host (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies and concentrations used were Alexa Fluor 488 goat anti-rabbit IgG (H+L) (Invitrogen, A11008, 1:500), and Alexa Fluor 594 goat anti-mouse IgG1 (Invitrogen ...