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Citations for Thermo Fisher :
3751 - 3800 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The purified RNA was used to synthesize cDNA following the RevertAid H Minus First Strand cDNA Synthesis Kit (ThermoFisher). The reaction mixture was incubated at 42°C for 1 h and then terminated by heating at 70°C for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... cDNA synthesis was performed by using Maxima H Minus First Strand cDNA Synthesis Kit (ThermoFisher Scientific, Waltham, MA, USA). Oligo (dT)18 and random hexamer primers were used in the reaction mixture according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 488 (Thermo Fisher Scientific, A-21206), and donkey anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2022Quote: ... and donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 594 (Thermo Fisher Scientific, A21207).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... adult WT C57BL/6J mouse testes were dissected and incubated at 37 °C for 6 h in either Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12, Thermo Scientific) or compound 5 (100 μM ...
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bioRxiv - Plant Biology 2022Quote: ... cDNA was synthetized by reverse transcribing 1 μg of total RNA using RevertAid H minus reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Ambion) was reverse-transcribed using Maxima H Minus cDNA Synthesis Master Mix with dsDNase kit (M1682; Thermo Fisher Scientific), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2023Quote: Cultured viruses were collected by centrifugation at 9,000 rpm for 1 h at 4°C (Sorvall ST8FR, Thermo Scientific) and resuspended in phosphate-buffered saline ...
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bioRxiv - Plant Biology 2022Quote: ... then incubated in 0.2x SSC for 2 h at 55°C and treated with RNase A (Thermo Scientific #EN0531) at 37°C for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... coupled to a heated electrospray ionization source (H-ESI) and a triple quadrupole mass spectrometer (TSQ Vantage, Thermo Scientific) operated under selected reaction monitoring (SRM ...
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bioRxiv - Bioengineering 2023Quote: ... was used as a primary antibody and HRP conjugated Goat anti-rabbit (H+L) IgG (Thermo Fisher Scientific, USA) was used as a secondary antibody for detecting target proteins ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 2 h incubation with fluorescent secondary antibodies (Fluor 568 labeled donkey anti-mouse (1:500; Life Technologies), Fluor 488 labeled donkey anti-rabbit (1:500 ...
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bioRxiv - Cell Biology 2023Quote: Tachyzoites or parasites exposed to 48 or 96 h differentiation stress as explained before were conserved in TriZol (Invitrogen) solution at −80°C until use ...
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bioRxiv - Biochemistry 2022Quote: ... After transfection with Lipofectamine 3000 and the indicated plasmids or siRNAs for 48 h according to the manufacturer’s instructions (Invitrogen), the transfection efficiency was assessed by Western blotting (WB ...
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bioRxiv - Immunology 2022Quote: ... Cell lysates were incubated with RNA-aptamer-coupled beads for 3.5 h at 4°C under rotation in the presence of 60U RNasin (Ambion). For each pull-down ...
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bioRxiv - Cancer Biology 2022Quote: ... fetal liver cells were thawed and cultured for 48 h in alpha-minimum essential media (alpha-MEM) Glutamax (Gibco) containing 10% FCS ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 2 µg/ml of polybrene) for 24 h and selected with 0.5 mg/ml of Geneticin/G418 Sulfate (Gibco) for at least one week ...
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bioRxiv - Cancer Biology 2023Quote: ... Corning® Bio-Coat® Matrigel® Invasion Chambers with 8.0 μm PET membranes were rehydrated for 2 h at 37 °C using NB-A medium with only 100 U/ml Penicillin 100µg/mL Streptomycin (Gibco) added ...
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bioRxiv - Developmental Biology 2022Quote: The ability of the tissues to produce testosterone was assessed after 3 h of incubation with DMEM/F12 (Gibco) containing 0.1% BSA ...
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bioRxiv - Immunology 2023Quote: ... washed with PBS and cultured for 48 h in 1ml of complete Roswell Park Memorial Institute (RPMI) 1640 (Gibco) containing 2 mM L-glutamine (Corning) ...
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bioRxiv - Cell Biology 2023Quote: ... for 2–5 h at 37°C followed by a 5 min incubation in TrypLE express (Thermo Fisher Scientific) to generate small clumps of corneal endothelial cells ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and after 16-h cells were left untreated or treated for 30 min with MitoTracker™ Orange CMTMRos (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice and incubated with donkey anti-murine IgG-(H+L)-AF488 (20 µg/mL, #A21202, Invitrogen) on ice for 30 min ...
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bioRxiv - Biochemistry 2023Quote: ... was pre-soaked in buffer H and then overlaid on a positively-charged nylon membrane (BrightStar™-Plus, Invitrogen). The protein-mRNA mixtures were applied onto a 96-well slot-blot apparatus and then filtered through the double membranes by vacuum ...
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bioRxiv - Cell Biology 2023Quote: ... cells were first incubated at 37 °C for 1 h in buffer H1 (50% formamide (FA, Cat. # AM9342, ThermoFisher), 10% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... organoids were passaged using H+++B Medium (Advanced DMEM/F-12 [Thermo Fisher], HEPES pH7.2-7.5 [10mM, Thermo Fisher] ...
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bioRxiv - Immunology 2023Quote: ... or horseradish peroxidase (HRP)-conjugated rabbit anti-guinea pig IgG (H+L) antibody (1:500; Invitrogen, Cat. PA1-28597) in blocking buffer for 1-1.5 hours at RT ...
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bioRxiv - Genomics 2023Quote: ... Reverse transcription was then performed on the RNA template using Maxima H-Reverse Transcriptase (Thermo Fisher, cat. no. EP0753) to create a DNA-RNA hybrid ...
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bioRxiv - Immunology 2023Quote: ... For qRT-PCR cDNA was synthesized using Maxima H Minus cDNA synthesis kit (catalog no. M1681; Thermo Fisher Scientific). PrimePCRTM PCR Primers assay plates were custom made with validated primers for use with EvaGreen dye-based chemistry (Bio-RAD ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1-2 h at room temperature (1:1000) for viral titration or an anti-GFP antibody (A11122, Invitrogen) for measurement of oxGFP-positive cells ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthetized by reverse transcribing 1 µg of total RNA using RevertAid H minus reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... and subsequently treated with 0.5 µL each of Rnase H and Rnase A (Thermo Fisher, EN0531 and NEB M0297S) for 30 min at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... and BN-PAGE gels were run for 2-2.5 h at 150 V using NativePAGE running buffer (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... DNA was allowed to dissolve for 48 h prior to quantification using Qubit dsDNA Quantitation range kit (Q32853, Thermofisher). From the estimated 3 to 6 million cells in each of these bins ...
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bioRxiv - Cancer Biology 2024Quote: ... After 48 h the mCherry-positive cells were sorted at Salk’s Flow Cytometry Core and Hoechst 33342 (Invitrogen, H3570) staining was used to determine cell viability ...
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bioRxiv - Neuroscience 2024Quote: ... then subjected to a 1 h incubation with secondary antibody (Goat anti-Rabbit IgG, 1:200, Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... three replicas of each cell line and condition were incubated for 24 h with 0.15 µg/mL of Colcemid (Gibco Thermofisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... at 4DC overnight and stained with goat anti-mouse IgG (H+L) Alexa Fluor 488 (1/800, Invitrogen, UK) and 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa Fluor 568 goat anti-rabbit IgG (H+L) secondary antibody (CAT NO. A-11011) was purchased from Invitrogen. Anti-IBA1 antibody was purchased from (CAT NO ...
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bioRxiv - Cell Biology 2024Quote: Liver tissue sections were processed for H&E and images were taken using Evos XL Core (Thermo Fisher Scientific). Liver tissue sections were first deparafinized by heating at 85°C for 10 min followed by treatment with decreasing gradients of alcohol ...
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bioRxiv - Genetics 2024Quote: ... embryos were then incubated with Goat anti-Mouse IgG (H+L) Alexa Fluor 488 (1:150, Cat# A28175, Invitrogen) overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... and pieces incubated in secondary antibodies [anti-rat secondary antibody (H+L) 1:400 (Thermo Scientific, Alexa Fluor 488), anti-rabbit 568 secondary antibody (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... The following secondary antibodies were used: goat anti-mouse IgG (H+L) cross-adsorbed Alexa Fluor 546 (RRID: AB_2534071, A-11003, ThermoFisher), goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2024Quote: ... followed by cross-absorbed goat anti-rabbit IgG (H\&\L) conjugated to either AlexaFluor 488 or 594 fluorophores (Invitrogen).
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bioRxiv - Plant Biology 2024Quote: ... GST and GST-NbSTIR1 were induced with 0.4 mM IPTG for 4 h at 37℃ and purified using Glutathione agarose resin (ThermoFisher). NbISIC1 and its mutant were induced with 0.1 mM IPTG for 16 h at 12℃ and purified using Ni-NTA agarose resin (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... After 2-4 h the coverslips were flipped over an astroglia feeder layer in Neurobasal medium (GIBCO - Life Technologies) supplemented with SM1 supplement (1:50 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... After 2-4 h the coverslips were flipped over an astroglia feeder layer in Neurobasal medium (GIBCO - Life Technologies) supplemented with SM1 supplement (1:50 dilution ...
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bioRxiv - Physiology 2024Quote: ... total RNA was reverse transcribed into cDNA using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, K1682) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... was used and RNA samples were converted to cDNA with RevertAid H Minus Reverse Transcriptase kit (Thermo Fisher Scientific) and analysed as previously described (Lemberg et al. ...
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bioRxiv - Microbiology 2024Quote: ... After 72 h BMDM were fixed with ice-cold methanol for 3 minutes and washed twice with PBS (Gibco); nuclei were then stained with DAPI (Invitrogen) ...