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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Samples were then washed with 1xPBS and incubated with μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies, #D1306). Coverslips were then mounted in Vectashield (LSBio ...
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bioRxiv - Immunology 2022Quote: ... with surrounding necrotic areas being carefully removed and were cut into small pieces of 2–4 mm and reserved in the mixture of 1X DMEM medium (Gibco) and Penicillin-Streptomycin solution (Hyclone) ...
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bioRxiv - Microbiology 2022Quote: ... The resulting pellets were resuspended in 2 mls PBS and dialyzed overnight at 4°C in 1L PBS using SnakeSkin 10,000 MWCO dialysis tubing (Thermo Fisher). Buffer was refreshed and samples were dialyzed again overnight ...
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bioRxiv - Cell Biology 2022Quote: ... MBL) and an additional 2 h at 4°C with 20 μL of a slurry of IgG-conjugated Dynabeads (Invitrogen). After washing the beads five times with IP buffer ...
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bioRxiv - Microbiology 2022Quote: ... E and S1 exposure were conducted in the presence or absence of neutralizing antibodies against toll-like receptor 2 and 4 (TLR2 and TLR4) antibodies (ThermoFisher Scientific Cat ...
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bioRxiv - Molecular Biology 2023Quote: ... and grown to an OD600 range of 2-4 at 30°C in a deep-well 96-well plate (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... an equal amount of protein lysates was subjected to SDS-PAGE 4– 12% gradient gels and electro transferred to a nitrocellulose membrane (iBlot 2, Invitrogen). Membranes were blocked with 5% non-fat milk ...
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bioRxiv - Neuroscience 2022Quote: Larval fish (4-7 dpf) were mounted in a small drop of 2% low melting point agarose (ThermoFisher Scientific 16520) in the center of the uncoated side of a mirror galvanometer (Thorlabs GVS0111) ...
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bioRxiv - Molecular Biology 2022Quote: ... endogenous PABP-mRNA complexes were immunoprecipitated from cleared lysates for 2 hours at 4°C using Dynabeads protein G (ThermoFisher) conjugated to anti-PABP (clone 10E10 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were then fixed in 4% formaldehyde at room temperature for 10 minutes and mounted onto slides using ProLong Gold or Diamond Antifade Mountant with 4′,6-diamidino-2- phenylindole (DAPI) (ThermoFisher). Images were obtained using a Leica SP8 confocal microscope using excitation/emission spectra ...
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bioRxiv - Cell Biology 2024Quote: ... a working solution of 0.83 mg/mL solution of sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-SANPAH, 22589; Thermo Fisher) in PBS was prepared from a stock solution of 83 mg/mL sulfo-SANPAH in DMSO (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2024Quote: ... Hippocampal sections were stained with 4’,6-diamidino-2-phenylindole (DAPI) and cresyl violet or fluorescent Nissl (NeuroTrace 435/455 Blue, ThermoFisher). OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in a flow-cytometry buffer and DAPI (4′,6-diamidino-2-phenylindole, 0.5 μg/ml; ThermoFisher #D1306) solution to exclude dead cells ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradient was centrifuged at 200.000×g for 2 hours at 4°C in a S52-ST rotor (Thermo Scientific) and subsequently fractionated into 14 fractions using the BioComp Piston Gradient Fractionator device ...
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bioRxiv - Genomics 2024Quote: ... and transfer plasmids were transfected at a mass ratio of 2:3:4 with Lipofectamine 3000 (Thermo Fisher Scientific L3000001) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... the sections were mounted with ProLong Gold with 4′,6-diamidino-2-phenylindole (DAPI) (catalog no. P36935, Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... 3×106 MART-1-transduced T cells were added and incubated for the indicated hours (2, 4 or 6) in “Intermediate” 4K6R medium consisting of RPMI 1640 Medium for SILAC (88365, Thermofisher) with 10% dialyzed fetal bovine serum (15605639 ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then pegylated for 15 minutes with 2 mM MS(PEG)4 Methyl-PEG-NHS-Ester (ThermoFisher Scientific) before grids.
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bioRxiv - Cell Biology 2023Quote: Binucleation analyses and detection of aberrant divisions were performed by heat-fixing cells on a microscope slide at 70 °C before staining with 4’,6-diamidino-2-phenylindole (DAPI) (SlowFade Diamond Antifade Mountant with DAPI, Invitrogen) and Calcofluor (Sigma) ...
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bioRxiv - Bioengineering 2023Quote: PAAm gels prepared on coverslips were transferred to multiwell plates before covering with 0.2 mg/mL sulfosuccinimidyl 6-(4’-azido-2’-nitrophenylamino)hexanoate (sulfo-SANPAH) (Thermo Fisher) solution ...
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bioRxiv - Genetics 2023Quote: ... Protein was separated on a 4–12% Bis-Tris gel and transferred using iBlot 2 transfer apparatus (Thermo Fisher Scientific). A 5% milk solution in TBST was used for blocking for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... TUBB4A and MUC5AC overnight at 4 °C and then incubated with Alexa 488-F(ab’)2 fragment of goat anti-mouse IgG (Invitrogen) for 2 h at room temperature ...
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bioRxiv - Biophysics 2023Quote: The membrane probes Di-4-ANEPPDHQ and Laurdan (6-dodecanyl-2-dimethylaminonaphtalene) were obtained from Molecular Probes (Eugene, OR, USA) and dissolved in ethanol and dimethyl sulfoxide (DMSO ...
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bioRxiv - Biochemistry 2023Quote: The cells were seeded in 6 well plate and treated the next day using 2 µg of plasmid and 4 µL of lipofectamine 3000 (Invitrogen) for 24 h or 10 nM of siRNA and 5 µL of RNAiMAX (Invitrogen ...
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Beclin1-Deficient Adipocytes Promote Tumor Progression by YAP/TAZ-dependent Adipocyte TransformationbioRxiv - Biochemistry 2023Quote: ... Colony formation assays were performed by mixing various numbers of differentiated adipocytes (incubated for 2 days in differentiation media and then 4 days with DMEM (Gibco), 1 μM insulin (I9278 ...
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bioRxiv - Cell Biology 2023Quote: ... The reactions were quenched with 2 μl of 2.7 M ammonium bicarbonate before loading on Bolt™ 4–12% Bis-Tris Plus gels (Invitrogen) for separation ...
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bioRxiv - Genetics 2023Quote: ... ISE6 cells were fixed with 4% paraformaldehyde followed by 1 x PBS washes and the coverslip was mounted on a slide using Antifade gold mounting reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen) and observed under the Nikon W-1 Spinning Disk confocal microscope ...
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bioRxiv - Immunology 2023Quote: ... intracellular staining was performed for 2 h at 4°C after stimulation for 2 h with PMA/Ionomycin Cell Stimulation Cocktail containing protein transport inhibitors (ThermoFisher). Data were acquired on an Aurora (Cytek Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... Antibody–protein complexes were recovered during 2 h incubation at 4°C with protein A/G magnetic beads (Thermo Scientific), followed by duplicate washes of 15 min with low-salt wash buffer (0.1% SDS ...
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bioRxiv - Bioengineering 2023Quote: ... in blocking buffer prior to a final incubation with 4′,6-diamidino-2-phenylindole (DAPI) or anti-phalloidin for F-actin (Invitrogen) at 25 °C ...
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bioRxiv - Physiology 2024Quote: ... CHX-treated cells were harvested at different time points (0, 2, 4 and 6 hours) and processed for immunoblotting with anti-LSD1 (Invitrogen) and anti-RCOR1 (Abcam ...
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bioRxiv - Biophysics 2024Quote: ... This was done by placing 0.2 mg/ml sulfo-succinimidyl-6-(4-azido-2-nitrophenyl-amino) hexanoate (sulfo-SANPAH) (Thermo Fisher) in 0.5 mM pH 8.5 HEPES buffer onto the hydrogel surface and irradiating with ultraviolet (UV ...
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bioRxiv - Cell Biology 2024Quote: ... No mycoplasma were detected in cultures by 4′,6-diamidino-2-phenylindole (DAPI) or TO-PRO-3 (Thermo Fisher Scientific) staining ...
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bioRxiv - Bioengineering 2024Quote: ... the sections were rinsed in PBS and the nuclei were counterstained and mounted with Slowfade gold antifade mountant containing 4’,6-diamidino-2-phenylindole (DAPI; #S36939, Invitrogen). The slides were imaged at a 4X magnification using EVOS M7000 (ThermoFisher Scientific™ ...
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bioRxiv - Cancer Biology 2024Quote: ... and A-253 cells were serially diluted and seeded at three different dilutions (2, 4 and 128 cells/well) with 50 µL serum-free DMEM/F12 medium (Gibco) supplemented with 10 ng/ml human EGF (R & D systems) ...
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bioRxiv - Immunology 2024Quote: ... filtered through a 0.45 µm pore-sized filter to remove cell debris and concentrated by centrifugation at 100000 g for 2 h at 4°C (Thermofisher WX Ultra80 centrifuge ...
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bioRxiv - Genetics 2024Quote: ... HEK293T cells cultured in 15-cm Petri dishes were grown to 60-70% confluency and then transfected with 4 µg of the UTR library of DNA templates (2 µg for each UTR library) using lipofectamine 3000 reagent (Invitrogen). Fourteen hours after transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 μg of protein (or all of the sample for the MDF multiplexing experiment across 2 gels) was loaded into a NuPAGE 4∼12% Bis-Tris 1.5 mm gel (Invitrogen #NP0335) and gel electrophoresis performed ...
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bioRxiv - Microbiology 2024Quote: ... Transfectants were selected with either 4 nM WR99210 (Jacobus Pharmaceuticals; pSLI) or 2 μg/μl blasticidin S (Life Technologies; pSLI2). SLI for selection of parasites with the plasmid integrated into the genome was done as described (Birnbaum et al. ...
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bioRxiv - Neuroscience 2022Quote: 5-ethynyl-2’-deoxyuridine (EdU; Invitrogen) (10 μM ...
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bioRxiv - Bioengineering 2019Quote: ... 5-Ethynyl-2′-deoxyuridine (EdU, Invitrogen) was prepared according to the manufacturer’s specifications and diluted in cell culture media to 10 μM ...
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bioRxiv - Neuroscience 2023Quote: ... 5-Ethynyl-2’-deoxyuridine (EdU, ThermoFisher) was injected into the vitreous chamber to label proliferating cells ...
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bioRxiv - Immunology 2023Quote: ... 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) was dissolved in sterile PBS (5 mg/mL) ...
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bioRxiv - Neuroscience 2023Quote: 5-ethynyl-2’-deoxyuridine (EdU; Invitrogen) was injected i.p ...
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bioRxiv - Neuroscience 2023Quote: 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) was dissolved at 0.2 mg/ml in the drinking water to detect proliferating cells ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.01 mg ml-1 hygromycin B (ThermoFisher) at 37°C and 5% CO2.
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bioRxiv - Molecular Biology 2020Quote: ... B-27 Serum-Free Supplement 1/100 (Gibco), 2mM L-glutamine (Gibco) ...
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bioRxiv - Bioengineering 2019Quote: ... and 1% amphotericin B (all acquired from Gibco). For all cell-related studies ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 1× B-27 supplement (Life Technologies) and 20 ng/mL FGF2 (Waisman Biomanufacturing ...
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bioRxiv - Systems Biology 2021Quote: ... and 1 μg/mL amphotericin B (Thermo Fisher). Leaflets were transferred to a 250 units type II collagenase (Worthington ...