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Citations for Thermo Fisher :
3601 - 3650 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Absorbance was measured at a wavelength of 450 nm using an ELISA microplate reader (Thermo Fisher). Assays were repeated at least three times.
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bioRxiv - Immunology 2023Quote: ... supernatant was collected and IFN-ɑ measured by ELISA according to manufacturer’s instructions (Invitrogen; catalog # BMS6027)
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bioRxiv - Microbiology 2023Quote: ... and the absorbance was measured at 450 nm in an ELISA plate reader (Varioskan, Thermo Scientific).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were washed and then developed by adding Slow Step ELISA TMB substrate (Thermofisher, Cat: 34024) to each well ...
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bioRxiv - Neuroscience 2024Quote: ... Levels of cerebral Aβ40/42 were determined by ELISA according to the manufacturer’s instructions (Invitrogen, CA). Furthermore ...
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bioRxiv - Immunology 2024Quote: ... IL-5 levels were quantified in undiluted BAL fluid by ELISA following the manufacturer’s instructions (Invitrogen).
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bioRxiv - Bioengineering 2024Quote: ... Cystatin A levels were determined by enzyme-linked immunosorbent assay (ELISA) according to manufacturer’s instructions (Invitrogen) and calculated using standard curve interpolation ...
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bioRxiv - Biochemistry 2024Quote: ... was measured by ELISA of secreted mIFN-γ in cell supernatants (Invitrogen REF 88-8314-22).
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bioRxiv - Neuroscience 2024Quote: ... then 5 μL samples were used to coat an ELISA plate (Nunc, MediSorp, Thermo Fisher Scientific) overnight at 4°C with agitation ...
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bioRxiv - Neuroscience 2024Quote: ... then 5 μL samples were used to coat an ELISA plate (Nunc, MediSorp, Thermo Fisher Scientific) overnight at 4°C with agitation ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μg RNA samples were reverse-transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). qPCR was performed using the Power SYBR® Green PCR Master Mix and an ABI Prism 7300 Sequence Detection System instrument and software (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... 2 mL of bacterial cultures were pelleted for RNA extraction with Max Bacterial Trizol kit® (Invitrogen) according to manufacturer protocol.
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bioRxiv - Systems Biology 2021Quote: ... 2 μg total RNA per sample was transcribed to cDNA by using SuperScript IV kit (Invitrogen, #18091050) with random hexamer primers (Thermo Fisher #SO142 ...
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bioRxiv - Bioengineering 2020Quote: ... EGM-2 was aspirated and cells were stained using the fluorescent LIVE/DEAD Viability/Cytotoxicity Kit (Invitrogen) according to kit instructions ...
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bioRxiv - Plant Biology 2021Quote: ... The cDNA was prepared from 2 µg RNA using a cDNA synthesis kit (SuperScript III; Invitrogen, USA). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cancer Biology 2020Quote: ... Complimentary DNA (cDNA) was synthesized from 2 ug of RNA using Superscript II Reverse Transcriptase kit (Invitrogen) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2021Quote: ... Confirmation of the aha5-2 mutant line was performed using TaqMan Gene expression Assay kits (Applied Biosystems) for HA5 (Assay ID At02274124_g1 ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 μg of RNA was used for cDNA synthesis using High-Capacity RNA-to-cDNA kit (Invitrogen). qPCR was completed with PowerUp SYBR Green Master Mix (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: A variant-specific RT-qPCR with the TaqMan™ SARS-CoV-2 kit Mutation Panel (Applied Biosystems) was used to screen the VOC/VOI mutations S_L452R and S_T478K following manufacturer ‘s protocol.
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bioRxiv - Neuroscience 2019Quote: ... #2:G378S) and controls were reprogrammed into iPSCs with CytoTune-iPS 2.0 Sendai reprogramming Kit (#A16517, ThermoFisher) and differentiated into MNs with the multistep protocol described by Ng (Ng et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 µg of total RNA was reverse transcribed using High Capacity cDNA Reverse Transcription kit (Invitrogen, USA) as per manufacturer’s instructions.
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bioRxiv - Plant Biology 2021Quote: ... cDNA was synthesized from 2 µg RNA using High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, USA). For each reaction 2.5 μl of cDNA (1:20 dilution) ...
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bioRxiv - Plant Biology 2021Quote: ... +2 no-template extraction control samples) were quantified using the Qubit dsDNA High Sensitivity Kit (Invitrogen, USA), and qualitatively evaluated by mixing ∼2 μL of each sample with 1 μL of GelRed (Biotium) ...
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bioRxiv - Molecular Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU)-incorporation assay has been performed using a commercially available kit (Invitrogen, C10340). Animals were injected intraperitoneally with 60 µl of EdU (0.5 mg/ml in 50% DMSO and 50% PBS solution ...
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bioRxiv - Microbiology 2022Quote: ... +2 no-template extraction control samples) were quantified using the Qubit dsDNA High Sensitivity Kit (Invitrogen, USA), and qualitatively evaluated by mixing ~2 μL of each sample with 1 μL of GelRed (Biotium) ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 μg of RNA from each fraction were poly(A) selected using Dynabeads mRNA DIRECT Kit (Invitrogen) as per manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... 2 μg of total RNA was reverse transcribed into cDNA using SuperScript III cDNA Synthesis kit (ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 2021 Total RNA was extracted from 2-4 day old female ovaries using the MirVana kit (Ambion). rRNA was depleted using antisense rRNA oligo hybridization with subsequent RNase H digestion ...
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bioRxiv - Immunology 2023Quote: ... The cytosol fraction was isolated from 2×107 BLaER1 cells using the Mitochondria Isolation kit (Thermo Fisher) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 µg of RNA from each fraction were poly(A) selected using Dynabeads mRNA DIRECT Kit (Invitrogen) as per manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... 2 μg total RNA was reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) and subjected to SYBR® Green chemistry-based qPCR using the QuantStudio 3 Real-time PCR System (Applied Biosystems ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 μg of RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... cDNA was synthesized from 2 µg RNA using the High-Capacity cDNA Reverse Transcriptase Kit (Thermo Scientific). 20 µL cDNA reactions were diluted 1:10 in UltraPure water ...
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bioRxiv - Plant Biology 2024Quote: ... 2 µl of purified RNA was reverse transcribed using the SuperScript IV Reverse Transcriptase Kit (Invitrogen; 18090050) with random hexamer priming ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 µg of RNA was converted to cDNA using the High-Capacity cDNA Reverse Transcription kit (ThermoFisher). RT-PCR was performed on cDNA using primers flanking the Mef2d α exons and the products were resolved on 5% polyacrylamide gels ...
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bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Cancer Biology 2024Quote: ... Cat No.DSHB-S1-810-(DSHB)-E7 anti beta tubulin supernatant 1ml).Proteins were detected using Goat Anti-Mouse IgG (H+L) peroxidase Conjugates (1;2500, Thermo scientific, Cat No. 31430) and Goat Anti-Rabbit IgG-HRP (1;1000 ...
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bioRxiv - Cell Biology 2024Quote: ... The following primary antibodies were used for western blotting: mouse monoclonal anti-beta actin 15G5A11/E2 (1:5000, Thermo Fisher, Cat# MA1-140), mouse monoclonal anti-CD63 8A12 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were assayed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™, Thermo Fisher Scientific, cat. no. V13154). Cells were plated in triplicate in a 96-well plate (5,000 cell/well) ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™, Thermo Fisher Scientific, cat. no. V13154). Cells were plated in triplicate in a 96-well plate (3,000 cell/well in 200 μL of complete medium and cultured under standard conditions for 48 h ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher)) supplemented with a protease inhibitor tablet (Roche ...
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bioRxiv - Genetics 2019Quote: ... 2 μl of probe (2 μM; Applied Biosystems) and 2 μl of template DNA (where extracts were normalised to 34 ng/μl) ...
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bioRxiv - Cell Biology 2020Quote: ... and Alexa Fluor 594 donkey anti-rat IgG (Invitrogen Molecular probes Cat#A21209).
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bioRxiv - Cell Biology 2020Quote: ... and Alexa Fluor 594 donkey anti-rat IgG (Invitrogen Molecular probes Cat#A21209).
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bioRxiv - Cell Biology 2020Quote: ... followed by anti-Rabbit/Mouse/Rat Alexa-dye coupled antibodies (1:400, Invitrogen). LysoTracker FarRed was used to visualize lysosomes in live microscopy (1:2000 ...
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bioRxiv - Immunology 2021Quote: ... and incubated overnight at 4°C with rat anti-mouse CD8 (4SM15, Invitrogen) and rabbit anti-mouse Ly6G (E6Z1T ...