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Citations for Thermo Fisher :
3801 - 3850 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Concentrations of proteins in plant leaves were determined according to the Pierce BCA Protein Assay Kit-Reducing Agent Compatible (Thermo Scientific, #23250, Rockford, IL).
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bioRxiv - Immunology 2020Quote: ... Monocytes were differentiated with GM-CSF (800U/ml) and IL-4 (500U/ml) in enriched RPMI (Gibco, 10% bovine serum, P/S, 2mM Glutamine) in a 5% CO2 humidified 37°C incubator as previously described (Geijtenbeek et al. ...
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bioRxiv - Physiology 2019Quote: ... The total protein of the kidneys was extracted by centrifugation at 14,000g for 15 minutes at 4°C and protein concentration was determined using micro bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, Rockford, Il, USA) according to manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2020Quote: ... incubated at RT for 10 min and the absorbance was recorded at 540 nm using a 96-well plate ELISA reader (Thermo Fisher, Rockford, IL, USA).57 The concentration of nitrite was calculated according to the standard curve generated from known concentrations of sodium nitrite dissolved in fresh culture media.
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bioRxiv - Cell Biology 2021Quote: ... Myofibrillar proteins were pelleted by centrifugation at 700g for 5min and the protein concentration of the sarcoplasmic fraction (supernatant) was determined via a bicinchoninic acid assay (Thermo Fisher Scientific, Rockford, IL). Samples were then diluted to equal concentrations in 1X Laemmli sample buffer and denatured at 95℃ for 5min ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were incubated for 12 to 15 hours and then washed with 2 mL of a 0.25 % trypsin solution (25200056, Gibco, Fisher Scientific, Hanover Park, IL, USA) in 1X EDTA ...
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bioRxiv - Biochemistry 2020Quote: ... The slides were incubated in a humidity controlled (50%) enclosure for 45 minutes and blocked by incubation with Superblock(tm) blocking buffer (Thermo Fisher Scientific, Rockford, IL) for 45 minutes at room temperature with shaking at 60rpm.
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bioRxiv - Developmental Biology 2021Quote: ... and centrifuged at 13000×g for 20 min at 4°C.The protein concentrate on of collected supernatant was determined using bicinchoninic acid (BCA) assay (23225; Thermo Fisher Scientific, Rockford, IL, USA). An equal amount of each protein sample was electrophoresed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2021Quote: ... IL-1β and TNF-α – levels in cell culture supernatants were used (#88-7066, #88-7106, #88-7261, #88-7346, Thermo Fisher Scientific, USA). ELISA kits are based on the sandwich immunoassay technique ...
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bioRxiv - Immunology 2022Quote: ... Pen/Strep (Biological Industries, Israel) and 3,000 IU/ml IL-2 (Chiron Novartis, New Jersey, USA) in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA). Cells were split or medium was added every 2 to 3 days ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... The membranes were washed with TBST (thrice for 5 minutes each) and developed using Supersignal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL, USA). The bands were visualized through ImageQuant LAS500 series (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h RT and incubated o/n with a primary antibody cocktail containing anti-GFP antibody (polyclonal chicken anti-GFP, 1:2000; #A10262, AB_2534023; Thermo Fisher Scientific, Rockford, IL, USA), anti-TH antibody (TH polyclonal rabbit anti-mouse ...
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bioRxiv - Neuroscience 2022Quote: ... The protein concentration of the supernatant for all protein samples was determined by Pierce BCA Protein Assay Kit (#23227, Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed once with PBS containing both calcium and magnesium and labeled with 0.5 mg of membrane-impermeable EZ-linkTM Sulfo-NHS-Biotin (Thermo Fisher Scientific, Rockford, IL, USA) per plate for 30 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... First-strand cDNA synthesis of Isolated RNA was carried out using random primers and high capacity cDNA reverse transcription kit (Thermo Fisher Scientific, IL, USA). The forward and reverse primers used for SARS-CoV-2 nucleocapsid were 5’-GTAACACAAGCTTTCGGCAG-3’ and 5’-GTGTGACTTCCATGCCAATG-3’ respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... The carbohydrate and protein levels in the food waste were quantified using the anthrone method [32] and the modified Lowry method (Thermo Fisher Scientific, IL, USA), respectively ...
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Dynamic Effects of Ventral Hippocampal NRG3/ERBB4 Signaling on Nicotine Withdrawal-Induced ResponsesbioRxiv - Neuroscience 2023Quote: ... frozen VH tissue was weighed and gently homogenized with a glass dounce tissue grinder in 10 vol (1:10, wt/vol) of Syn-PER synaptic protein extraction reagent (Thermo Fisher Scientific, Rockford, IL) supplemented with a protease and phosphatase inhibitor cocktail ...
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... The total proteins were in the supernatant and normalized to 1mg/mL based on the Micro BCA assay according to manufacturer’s recommendations (Thermo Fisher Scientific Inc, Rockford, IL). 25 μL of each sample was diluted in the Diluent Buffer to 100 μL and added in the EGFR capture plate ...
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bioRxiv - Microbiology 2023Quote: ... 60 L of seawater were collected from 5 m depth in the center of the Gulf of Trieste and aged in acid-washed and Milli-Q water-rinsed 20 L Nalgene carboys (ThermoFisher Scientific, Rockford, IL, USA) for about one month at room temperature in the dark ...
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bioRxiv - Microbiology 2023Quote: The abundance of respiring bacterial cells was determined at a single-cell level using BacLight Redox Sensor Green Vitality Kit (ThermoFisher Scientific, Rockford, IL, USA). The Redox Sensor Green (RSG ...
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bioRxiv - Immunology 2023Quote: 1 × 106 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 or PBS for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before being flash frozen in liquid nitrogen ...
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bioRxiv - Immunology 2023Quote: 5×105 NIKWT and NIKKO BMDMs were stimulated with 20ng/mL IL-4 for 6 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were spun down and washed once with PBS before total RNA was isolated from cells by Purelink™ RNA Mini Kit (Life Technologies) ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were stimulated with IL-4 (20ng/mL) for 24 hours before lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). 75,000 DiO (ThermoFisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were stimulated with IL-4 (20ng/mL) for 24 hours before being lifted with PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were diluted to a concentration of 1 million cells/mL and 50uL of cell solution was plated on a precoated 4ug/mL fibrinogen and 0.5% polyvinyl alcohol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were treated with 20ng/mL IL-4 for 5 hours before cells were detached using PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific; Waltham, MA, USA). Cells were then stained with a combination of Mitotracker Green FM ...
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bioRxiv - Plant Biology 2023Quote: ... The quantity and quality of DNA were confirmed by electrophoresis on agarose gels stained with GelRed and visualized under UV light using a NanoDrop 1000 spectrophotometer (Thermo Scientific, Rockford, IL, USA). Nine samples (three from each treatment ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with Alexa Fluor-conjugated secondary antibodies (fluorescence at 488, 594, 555, or 645 nm; Thermo Fisher Scientific, Rockford, IL, USA). Nuclei were counterstained with DAPI (1:10,000 in PBS ...
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bioRxiv - Immunology 2023Quote: ... or IL-2 combined with spike protein for 16 hrs and used for RNA extraction using TRIzol™ reagent (Thermo Fisher Scientific, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The RdRp P/L complexes were purified using Ni-NTA affinity chromatography based on their respective eight-histidine tag as shown in Table 1 (Thermo Scientific, Rockford, IL, USA). The pET-15b (Novagen ...
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bioRxiv - Cell Biology 2024Quote: Organoids were treated from day 4 through day 11 for EN1 and day 7 through day 11 for EPC2 with or without 10 ng/mL recombinant IL-13 (200-13, Thermo Fisher Scientific, Waltham, MA), reconstituted in phosphate-buffered saline (PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse monoclonal V5 (1:5000, Thermo Fisher Scientific, R960CUS), mouse monoclonal HDAC1 (1:1000 ...
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bioRxiv - Cancer Biology 2021Quote: ... and Alexa 598 goat anti-mouse/rabbit (Molecular Probes) were used as secondary antibodies ...
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bioRxiv - Cancer Biology 2021Quote: ... AlexaFluor 488 conjugated to donkey anti-mouse (Life Technologies), and AlexaFluor 568 conjugated to donkey anti-mouse (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-ERK 1:100 (Invitrogen, 14-9108-80), rabbit anti-pERK 1:500 (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... and goat anti-mouse IgG1 Alexa 547 (Invitrogen, A21123) (1:100 ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies used were goat anti-mouse (Invitrogen, 31432) and goat anti-rabbit (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... mouse monoclonal Anti-APP antibody (1:100, Thermo Fisher Scientific Cat# 13-0200 ...
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bioRxiv - Microbiology 2020Quote: ... a mouse monoclonal p53 Antibody (PAb 240, Invitrogen, USA), a mouse monoclonal antibody DAXX (sc-8043 ...
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bioRxiv - Cell Biology 2019Quote: ... monoclonal mouse anti V5 (Invitrogen R960-25, 1:2000), mouse monoclonal anti-yeast PGK (22C5D8 ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse Alexa Fluor 647 (Invitrogen, A21235, 1:1000) and anti-rabbit Alexa Fluor 647 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse Alexa Fluor 555 (Invitrogen, A21422, 1:1000), anti-rabbit Alexa Fluor 555 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... 488 donkey anti-mouse (1:400) from Invitrogen (A32766); anti-rabbit IgG (1:3000 ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti GFP (Thermo Scientific, A-11120, clone 3E6) 1:750 ...
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bioRxiv - Cell Biology 2020Quote: ... AlexaFluor568-conjugated donkey anti-mouse IgG (ThermoFisher Scientific A10037), AlexaFluor-594 donkey anti-rat IgG (ThermoFisher Scientific A-21209) ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-mouse-Alexa 568 (1:400, Life Technologies). The following compounds were used in this study ...
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bioRxiv - Cell Biology 2020Quote: ... or Alexa594-conjugated goat anti-mouse (1:2000; Invitrogen), respectively ...
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bioRxiv - Cell Biology 2020Quote: ... or goat-anti-mouse (1:1,000 dilution, #A32727, Invitrogen) and Alexa-488-conjugated goat-anti-rabbit (1:1,000 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... primary and a 488-nm anti-mouse IgG (Invitrogen) with a 594-nm anti-rabbit IgG (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse Alexa Fluor 488/800 (Invitrogen, CA, USA), anti-rabbit IgG-horseradish peroxidase (HRP ...