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Citations for Thermo Fisher :
3651 - 3700 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentration for all samples (culture media, PBS rinses, cytosol, and pellet) was measured using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford IL) and 20 µg of protein from each sample was run on an SDS PAGE gel and transferred to a PVDF membrane ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the supernatant was collected and the cleared lysate protein concentration was quantified using the BCA protein assay kit (Thermo Scientific, Pierce Rockford, IL) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: Primary human CD4+ T cells were incubated with 20U/ml interleukin-2 (IL-2) alone or in combination with α-CD3/CD28 antibody-coated beads (100 μL/30 x 106 cells) (Gibco/Thermo Fisher) for 72 hours prior to infection with 100ng of p24 equivalent of pseudotyped pMorpheus-V5 ...
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bioRxiv - Molecular Biology 2022Quote: ... total protein was measured from SEC fractions using a bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, IL) with an automated KoneLab instrument (Thermo Scientific, Vantaa, Finland). Based on total protein ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoprecipitated protein was processed for western blotting as previously published (24) and developed with Clean-blot™ IP detection reagent (HRP) (Thermo Scientific, IL). Antibodies used for western blotting are listed in supplementary table S7.
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bioRxiv - Neuroscience 2021Quote: ... 0.5% sodium deoxycholate and 0.1% SDS plus protease and phosphatase inhibitor cocktails (Cat. Nos. 78430 and 78428, respectively; Thermo Fisher Scientific, Rockford, IL). Tissue homogenates were sonicated and centrifuged as described in detail in our previous reports (13 ...
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bioRxiv - Molecular Biology 2020Quote: ... were analyzed on a high resolution Q Exactive HF Hybrid Quadrupole - Orbitrap Mass Spectrometer equipped with a heated electrospray ionization (HESI)-II source (Thermo Scientific, Rockford, IL) and a Vanquish Horizon UHPLC system (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Ten- to eleven-week-old mice were anesthetized with a mixture of ketamine (Zoetis, Kalamazoo, MI) and xylazine (Akorn Animal Health, Lake Forest, IL) and inoculated intranasally with either PBS (Gibco, Grand Island, NY), 1×104 PFU ...
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bioRxiv - Immunology 2022Quote: ... IL-5 and IFN-γ were quantified using Invitrogen™ eBioscience™ ELISA Ready-SET-Go!™ (Thermo Fisher Scientific, Waltham, MA, USA). IP-10 (CXCL10) ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were cultured at density of 10 embryos in 10 µl KSOM medium supplemented with 3 mg bovine serum albumin/mL (14) in 60-well plates (LUX 5260, Nunc, Naperville, IL, USA) overlaid with 2 mm of heavy paraffin oil (Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... The supernatant from each tube was isolated and protein concentration for each sample was quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL). 20 µg of each sample was loaded onto a 4-20% Gel (Bio Rad ...
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bioRxiv - Immunology 2022Quote: ... The proteins were resolved by SDS-PAGE on 4-12% NuPAGE gels using NuPAGE MOPS buffer (NuPage gels, Thermo Fisher Scientific, Rockford, IL), and transferred to methanol-activated PVDF membrane (Perkin Elmer ...
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bioRxiv - Cell Biology 2022Quote: ... the concentration of total soluble proteins of each sample was determined using BCA™ Protein Assay (Thermo Scientific™ Pierce™, Rockland, IL). To run a SDS gel ...
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bioRxiv - Immunology 2022Quote: ... washed and added at 103 cells per well to various ratios of effector T cells (CTC or UTC population after overnight incubation with IL-15) in 96 well V-bottomed plates (NUNC, Thermo Fisher Scientific). After 4 hours of co-incubation ...
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bioRxiv - Cell Biology 2019Quote: ... Cells cultured in 24-well plates were washed with phosphate-buffered saline and treated with 0.2 ml of Mammalian Protein Extraction Reagent (Thermo Scientific, Rockford, IL, USA). An aliquot of the solubilized cell solution (4 μl ...
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bioRxiv - Cancer Biology 2019Quote: ... Three 15 amino acid peptides (P1, P3 and P4) and one 14 amino acid peptide (P2) were synthesized (Thermo Fisher Scientific, Rockford, IL). The purity of the peptides was >95% and each peptide was modified by N-terminal acetylation and C-terminal amidation to enhance their stability.
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bioRxiv - Physiology 2019Quote: ... The final RNA yield and purity were evaluated by the absorption ratio A260/A280 measured with a Nanodrop (Thermo Scientific, Rockford, IL, USA). The synthesis of cDNA for reverse transcription was carried out with a Protoscript II first strand cDNA synthesis kit (New England Biolabs ...
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bioRxiv - Cell Biology 2019Quote: ... 1μg of RNA was reverse transcribed using oligo-DT and SuperScript III First-Strand Synthesis System (18080051, Thermo Fisher Scientific, Rockford, IL, USA). Relative qRT-PCR was performed in a total volume of 25 µl with 12.5 µl SYBER GREEN master mix (1725121 ...
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bioRxiv - Immunology 2019Quote: Ab019 and Ab161 were purified by incubation with Protein G beads and their concentrations were determined by BCA protein assay kit (Thermo Scientific, IL, USA). According to the manufacturer’s manual ...
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bioRxiv - Neuroscience 2019Quote: ... the same protocol to collect the hemolymph was performed and measurements were done in the concavity made in the parafilm that contains the hemolymph drop with a microelectrode (Orion 9810BN, Thermo Scientific, IL, USA). We repeated the protocol in 20 different samples.
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bioRxiv - Immunology 2019Quote: ... Hemocytes nuclei were then stained by incubating with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Thermo Fisher Scientific, Rockford, IL, USA) dissolved in PBS and washed thrice with PBS ...
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bioRxiv - Cell Biology 2020Quote: The concentration of IFNλ1/IL-29 was measured in basolateral medium from RSV BT2a- or mock-infected cultures at 96 hpi by ELISA (Thermo Fisher Scientific; BMS2049). The manufacturer’s instructions were followed.
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bioRxiv - Immunology 2019Quote: ... The selected combinations of hrproteins or peptide demonstrated the highest reduction in HIV-1 p24 release without inducing tissue toxicity as indicated with a LDH assay (Pierce LDH Cytotoxicity Assay, Thermo Scientific, Rockford, IL).
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bioRxiv - Physiology 2021Quote: ... and the powder demineralized with a 0.5M EDTA solution at pH 8.0 containing protease inhibitors (Pierce Protease Inhibitor Tablets, EDTA-free, Thermo Fisher Scientific, Rockford, IL, USA) for 12 days under agitation at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Fibroblasts cultures were culture under standard conditions in MEM medium supplemented with 10% fetal bovine serum (Thermo Scientific, Rockford IL, USA, Ref. 12657) and 1% penicillin/streptomycin (Lonza ...
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bioRxiv - Biochemistry 2021Quote: ... HCV nsp5b RdRp was purified using Ni-NTA affinity chromatography based on the C-terminal eight-histidine tag according to the manufacturer’s specifications (Thermo Scientific, Rockford, IL, USA). NiV RdRp P/L complex coding sequence (AEZ01385 and AEZ01390 ...
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bioRxiv - Biochemistry 2021Quote: ... NiV RdRp P/L complex was purified using Ni-NTA affinity chromatography based on the P protein N-terminal eight-histidine tag according to the manufacturer’s specifications (Thermo Scientific, Rockford, IL, USA). The protein identities of the purified HCV nsp5b RdRp and NiV RdRp P/L complex were confirmed by mass spectrometry (MS ...
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bioRxiv - Immunology 2021Quote: ... Interleukin-8 (IL-8 or CXCL8) protein in the basolateral media was measured using a magnetic bead-based ELISA (Procartaplex, ThermoFisher Scientific, Waltham, MA) according to the instructions provided by the manufacturer and the plate were then read using a Luminex-based Bio-plex Multiplex system (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... Mandibular mesenchyme (i.e., cell number per mandibular primordium) was counted using a hemacytometer (1492, Hausser Scientific Partnership, Fisher Scientific, Hanover Park, IL, USA) in combination with MIPAR software (Tyler and Hall ...
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bioRxiv - Developmental Biology 2021Quote: Full-length cDNA synthesis from RNA was performed using Maxima H Minus reverse transcriptase (K1651, Thermo Scientific, Fisher Scientific, Hanover Park, IL, USA) following the manufacturer’s directions with 2 µg of total RNA and 100 pmol of d(T)20 VN primer ...
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bioRxiv - Microbiology 2020Quote: TaqMan RT-PCR was performed with the N2 primer/probe set and BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fischer Scientific) [31] using the QuantStudio 5 system (Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Microbiology 2020Quote: ... and cultured in Eagle’s medium (MEM, Fujifilm Woko Chemicals, Japan) supplemented with 5% heat-inactivated fetal bovine serum (FBS, Thermo Fisher Scientific, Rockford, IL), 1% (w/v ...
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bioRxiv - Microbiology 2019Quote: ... Lysozyme hydrochloride, from egg white (Wako Pure Chemical Industries, Osaka, Japan) was labeled with DyLight 488 NHS Ester (Thermo Fisher Scientific, Rockford, IL), according to the instruction.
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bioRxiv - Immunology 2021Quote: Expanded Tregs or Tconvs were activated with IL-2 (100 IU/mL) and 1:1 anti-CD3/anti-CD28-coated beads (Gibco Dynabeads T-Expander) for 4 d ...
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bioRxiv - Immunology 2021Quote: ... Surface-labeled cells were then washed with 1x perm-wash buffer and labeled with anti-IL-2-PE-Cy7 (Clone MQ1-17H12, Invitrogen 25-7029-42), anti-TNFα-PE (Clone Mab11 ...
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bioRxiv - Immunology 2020Quote: Cells were plated and allowed to adhere to the cover glass of a 4-well or a 1-well chamber (Nalge Nunc International, Naperville, IL) precoated with Fibronectin (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... CD4+CD25- T cells were stimulated with anti-CD3/anti-CD28 as described above in the presence of 20 ng/ml IL-2 (eBioscience, ThermoFisher Scientific, Langenselbold, Germany) and 5 ng/ml TGF-β1 (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: ... and the protein content was determined using the colorimetric micro–BCA Protein Assay Kit according to the instructions supplied by the manufacturer (ThermoFisher Scientific, Rockford, IL). The AssayMAP Bravo platform (Agilent Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... and lung cryosections after blocking were stained with a 1:200 dilution of Alexa Fluor 488 Phalloidin (Thermo Fisher Scientific, Rockford, IL, USA) for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Pen/Strep (Biological Industries, Israel) and 3,000 IU/ml IL-2 (Chiron Novartis, New Jersey, USA) in RPMI 1640 medium (Gibco, Thermo Fisher Scientific, Waltham, MA). Cells were split or medium was added every 2 to 3 days ...
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bioRxiv - Microbiology 2022Quote: ... Protein concentration of NAD+ or NADH extracts was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, cat. no. 23225, Rockford, IL) to normalize NAD(P)H and NAD(P)+ concentrations.
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was exposed to an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) (PierceTM ECL Western; Thermo Scientific. Rockford, IL, USA). Bands were visualized and quantitated using the Chemi DocTM MP imaging system (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was dissected and homogenized using a modified Pierce RIPA lysis and extraction buffer (RIPA+) (catalog#89900, Thermo Scientific, Rockford, IL, USA) that contains a Halt Protease Inhibitor Cocktail (catalog#1861281 ...
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bioRxiv - Neuroscience 2023Quote: The surface proteins of HEK293 and hippocampal cells were biotin-labeled and isolated using a Pierce cell surface protein biotinylation and isolation kit (A44390, Thermo Scientific, IL, USA). The protein samples were resolved using 10% SDS PAGE and transferred to polyvinylidene fluoride membranes ...
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bioRxiv - Immunology 2024Quote: Immunoprecipitation of cleaved IL-1β from uterine tissue lysates and amniotic fluid was performed using the Pierce™ Classic IP Kit (Cat# 26146; Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... and protein content of the remaining lysate was measured for normalisation of results (BCA Protein Assay Kit; #23225, Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Microbiology 2024Quote: ... Hyphae that were analyzed with the Lionheart FX automated microscope were inoculated in 96-well plates (#167008; Thermo Fisher Scientific; Rockford, IL, US) in 200 µl medium per well ...
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bioRxiv - Molecular Biology 2024Quote: ... Total protein content was determined using the bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, IL) with an automated KoneLab instrument (Thermo Scientific, Vantaa, Finland).
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bioRxiv - Genomics 2024Quote: ... Thin sections were cut with a Leica UC7 ultramicrotome (Buffalo Grove, IL) prior to viewing at 120 kV on a FEI BT Tecnai transmission electron microscope (ThermoFisher/FEI, Hillsboro, OR). Digital images were acquired with a Gatan Rio camera (Gatan ...
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bioRxiv - Pathology 2023Quote: ... OCT embedded tissues were sectioned at 10 μm thickness and glycol methacrylate embedded at 3 μm and transferred to positively charged glass slides (Superfrost glass slides, Thermo Fisher, IL, USA). Hematoxylin/eosin (H&E ...