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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: MDA-PCa-2a was cultured in BRFF-HPC1 media (AthenaES, Baltimore, MD) with 20% fetal bovine serum (FBS, Hyclone Labs, Thermo Scientific, Rockford, IL) and 0.1% penicillin-streptomycin (Hyclone Labs ...
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bioRxiv - Physiology 2023Quote: ... Protein concentration was determined by the bicinchoninic acid protein assay (Smith et al., 1985) using bovine serum albumin as the standard (Pierce, ThermoFisher, Rockford, IL, USA).
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bioRxiv - Molecular Biology 2023Quote: ... Tandem Mass Tag 6-plex labelling of eluted RNA-binding proteins was performed using the TMTsixplex Isobaric Mass Tagging Kit (Thermo Scientific; Rockford, IL) and analysis by liquid chromatography tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Immunology 2023Quote: ... suspended in 100 mL of sodium carbonate buffer (pH 9.6) was used to coat each well of a 96-well plate (Nalge Nunc Inc., Naperville, IL, USA) that was incubated overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... The protein concentrations of the obtained OMVs and OMPs were quantified using a bicinchoninic acid (BCA) assay kit (Thermo Fisher, Rockford, IL, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... 20,000 single iPSCs were seeded onto each well of a U-botton 96-well plate (NucleonTM SpheraTM, Thermo Fisher Scientific, Rockford, IL, USA) to form embroid bodies with mTesR plus medium and 10μM ROCK inhibitor Y-27632 (Tocris Bioscience ...
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bioRxiv - Microbiology 2023Quote: ... After lysis in breaking buffer (20 mM potassium phosphate, 5 mM EDTA, 1 mg mL−1 lysozyme, 1 Pierce Protease Inhibitor Tablet (Thermo Scientific, Rockford, IL), 1 mM DTT ...
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bioRxiv - Genetics 2023Quote: ... and total protein concentration was estimated using Pierce Bicinchoninic Acid (BCA) protein assay kit according to the manufacturer’s protocol (cat. 23225, Thermo Fisher Scientific, IL, USA).
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bioRxiv - Genetics 2023Quote: ... Separated proteins were transferred onto a nitrocellulose membrane using an iBlot™2 Gel transfer device (cat. IB21001, Thermo Fisher Scientific, IL, USA). Non-specific protein binding to the membranes was blocked with 5% non-fat dry milk (cat ...
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bioRxiv - Immunology 2024Quote: Immunoprecipitation of cleaved IL-1β from uterine tissue lysates and amniotic fluid was performed using the Pierce™ Classic IP Kit (Cat# 26146; Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Total protein content was determined using the bicinchoninic acid protein assay (Pierce Biotechnology, Rockford, IL) with an automated KoneLab instrument (Thermo Scientific, Vantaa, Finland).
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bioRxiv - Physiology 2024Quote: ... and protein content of the remaining lysate was measured for normalisation of results (BCA Protein Assay Kit; #23225, Thermo Fisher Scientific, Rockford, IL).
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bioRxiv - Immunology 2023Quote: ... fresh media was supplemented with 30 U/mL of IL-2 and a 1:1 ratio of anti-CD3 and -CD28 Dynabeads (Thermo Fisher Cat# 11131D) and similarly treated with aphidicolin ...
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bioRxiv - Pathology 2023Quote: ... OCT embedded tissues were sectioned at 10 μm thickness and glycol methacrylate embedded at 3 μm and transferred to positively charged glass slides (Superfrost glass slides, Thermo Fisher, IL, USA). Hematoxylin/eosin (H&E ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting peptides obtained from soluble or membrane protein fractions were purified by C18 solid phase extraction using the C18 Spin column (Pierce Biotechnology, Thermo Scientific, Rockford, IL) and per the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... samples were clarified by centrifugation at 13.000 rpm for 15 min at 4 °C and protein concentration was determined using the BCA assay (Thermo Scientific, Rockford, IL, USA). Equal amounts of protein (40 – 50 μg ...
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bioRxiv - Genetics 2021Quote: ... incubated with mouse anti-HA antibody and then with the secondary antibody conjugated with Donkey-anti-mouse Alexa 567 (Invitrogen Molecular Probes). DNA was stained with 0.5 mg/ml DAPI (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2021Quote: ... was incubated with 10 µL of mouse polyclonal anti-SmHDAC8 or mouse polyclonal anti-SmRho1 crosslinked to protein-L Agarose beads (Thermo Scientific) overnight at 4°C on a rotator ...
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bioRxiv - Neuroscience 2020Quote: ... biotinylated anti-mouse IgG (H+L) made in horse and biotinylated anti-mouse IgG2a made in goat (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... biotinylated anti-mouse IgG (H+L) made in horse and biotinylated anti-mouse IgG2a made in goat (Life Technologies, ThermoFisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... with goat anti-mouse Alexa-488 (1:1000) and rabbit anti-mouse Alexa-594 (A27027 from Thermo Fisher Scientific, 1:1000) antibodies ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... anti-rabbit Alexa Fluor 488, Invitrogen, A11008; anti-mouse Alexa Fluor 488, Invitrogen, A11001; or anti-mouse Alexa Fluor 546, Invitrogen, A11030) for 1 hour at room temperature ...
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Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligationbioRxiv - Microbiology 2020Quote: ... Cleaved GP was detected with the primary mouse RBS-specific anti-GP antibody MR72 followed by secondary anti-mouse antibody-Alexa 555 fluorophore conjugate (Thermo Scientific). To investigate transport of IgGs bound to VSV particles into NPC1+ endosomal compartments ...
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bioRxiv - Cell Biology 2021Quote: ... 25uM Glutamax and 5% FBS to mouse Neuron culture media (same as mouse Plating media without FBS in ratio of 1:1. (Gibco #17504044)
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bioRxiv - Neuroscience 2021Quote: ... as well as secondary antibodies (Goat anti-mouse Alexa 555 or Goat anti-mouse Alexa 647, Thermo Fisher Scientific, 1:400) were diluted in GDB buffer containing 0.1% gelatin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times with PBS and then incubated with secondary antibody (1:1000 goat anti-rabbit antibody or goat anti-mouse antibody conjugated to Texas Red and 1:1000 goat anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies) and 1:1000 donkey anti-goat antibody conjugate to Texas Red (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... incubated with an anti-mouse antibody produced in goat and labeled with a goat anti-mouse antibody at 1:200 (Molecular Probes, tetramethylrhodamine ...
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bioRxiv - Bioengineering 2019Quote: ... Mouse-anti-His6 primary antibody and goat anti-mouse IgG H&L (Alexa Fluor® 488) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genetics 2019Quote: ... and incubated with 1:50 mouse anti-wg (4D4, DSHB) primary antibody and 1:500 anti-mouse 488 (A-21202, Molecular Probes) secondary antibody ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed (5x 5 min with PBST) then incubated in secondary antibodies against mouse (1:500 goat anti-mouse, ThermoFisher A11001) and rabbit (1:500 goat anti-rabbit ...
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Intestinal Enteroid Monolayers Model the Human Intestinal Environment for Escherichia coli InfectionbioRxiv - Microbiology 2021Quote: ... Secondary AlexaFluor antibodies (488 Goat Anti-Mouse, cat. A11001, Life Technologies, 488 Goat Anti-Rabbit, cat. A32731, Invitrogen, 660 Goat Anti-Mouse, cat. A21055, Invitrogen, 660 Goat Anti-Rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with appropriate secondary antibodies (488 anti-chicken, 546 anti-mouse, 647 anti-guinea pig, 647 anti-rabbit, 647 anti-mouse, Alexa Fluor, Life Technologies)
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bioRxiv - Developmental Biology 2021Quote: ... The PAIso-seq1 libraries for mouse oocytes were constructed with total RNA prepared from mouse oocytes using TRIzol reagent (Life Technologies). The libraries sequenced using PacBio Sequel II instruments at Annoroad.
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bioRxiv - Microbiology 2020Quote: ... They were then washed 3 times with PBS and incubated with goat anti-mouse conjugated to Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Thermo Fisher) containing DAPI in immunofluorescence buffer for an additional incubation of 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from wildtype adult C57BL/6 mouse spleen and lymph nodes using the Dynabeads FlowComp Mouse Pan T (CD90.2) Kit (Thermo Fisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: The Tregs and the CD4+/CD25- T cells were isolated from C57BL/6 mouse spleen using the Mouse CD4+CD25+ Treg Cells Kit (Invitrogen 11463D). The CD4+/CD8+ cells were sorted from testis ...
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bioRxiv - Microbiology 2022Quote: ... Immunostaining of NS1 and TRIM25 was realized by using the following antibody combination: mouse anti-NS1/goat anti-mouse Alexa 488 (ThermoFisher Scientific) and rabbit anti-TRIM25 (abcam)/goat anti-rabbit Atto647N (sigma) ...
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bioRxiv - Microbiology 2022Quote: ... For determination of infection rates employing wide field fluorescence microscopy the following antibodies in combination with a DAPI counter stain were used: mouse anti-nucleoprotein/goat anti-mouse Alexa488 (ThermoFisher Scientific). Cells grown on cover slips were mounted on microscopy slides using Mowiol.
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bioRxiv - Molecular Biology 2023Quote: ... The anti-FLAG antibody was detected using an anti-mouse Alexa 647 secondary antibody (Alexa 488 goat anti-mouse, 1:500 dilution, RT, 1 h, Invitrogen, #A11029). The images were captured with an InCell2000 imager.
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody: donkey anti-mouse Alexa 594 antibody, ThermoFisher), and with fluorescent Nissl and DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... phospho-tau (Ser202, Thr205) mouse monoclonal antibody (AT8, catalog #MN1020), and tau mouse monoclonal antibody (HT7, catalog #MN1000) from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: BV-2 cells were fixed with 4% PFA for 10 minutes and subsequently incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blotting was performed with 1:5000 dilution of mouse monoclonal anti-DBP5 and 1:10000 dilution of mouse monoclonal anti-GAPDH (Thermo Fisher) primary antibody overnight at 4°C and 1:10000 anti-mouse DyeLight 650 secondary to detect protein.
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bioRxiv - Biophysics 2024Quote: ... blocked with 1% BSA and stained with primary Anti-mouse laminin α5 (gift from Lydia Sorokin) and secondary Anti-mouse IgG AF594 (Invitrogen, A11005). Between all steps ...
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bioRxiv - Cell Biology 2023Quote: ... followed by PBS-Tween washes and secondary antibody incubation (2h at room temperature): Horseradish peroxidase secondary antibodies: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen® ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed and then incubated with Horseradish peroxidase secondary antibodies against the last antibody: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen ® ...
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bioRxiv - Molecular Biology 2023Quote: ... blocked with 1 %BSA/ 1%DMSO/PBS for 1 hr at room temperature and immunostained with mouse anti-SV2 AB2315387 and secondary Alexa Fluor 555 anti-mouse antibody from Molecular probes, Eugene.
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bioRxiv - Physiology 2023Quote: ... or SC71-S (1:25, Mouse IgG1, DSHB) followed by Alexa Fluor 488 or 647 (1:500, Goat anti-mouse, ThermoFisher Scientific). Images were acquired using a confocal microscope (Zeiss Axiovert 200 ...
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bioRxiv - Cell Biology 2023Quote: Mouse embryonic fibroblasts (MEFs) were isolated from E13.5 mouse embryos and grown in ‘MEF medium’ containing DMEM (41966029, Thermo Fisher Scientific) supplemented with 10% FBS (10270106 ...
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bioRxiv - Microbiology 2023Quote: ... berghei infected HepG2 cells were immunolabeled using anti-PbHSP70 (1:200, 2E6 mouse mAb) (62)to mark EEFs followed by donkey anti-mouse Alexafluor488 (Invitrogen A21202). MSP1 was immunolabeled with Anti-PBANKA_083100 ...