Labshake search
Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for IL 1beta Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... embryos were incubated for 2h at RT with Alexa Fluor 488 nm anti-mouse or Alexa Fluor 568 nm anti-mouse and Alexa Fluor 647 nm anti-goat secondary antibodies (Life Technologies) along with DAPI counterstaining (Invitrogen) ...
-
bioRxiv - Plant Biology 2024Quote: ... goat anti-mouse Alexa Fluor Plus 488 and F(ab’)2-goat anti-mouse Alexa Fluor Plus 555 from Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... and HRP-conjugated rabbit anti-mouse secondary antibody (#31430) and HRP conjugated Goat anti-mouse IgM (#31440) for immunoblotting were from Thermo Fisher. APC conjugated anti-human ganglioside GD2 antibody (#357306 ...
-
bioRxiv - Cell Biology 2019Quote: ... and subjected to immunoprecipitations with goat anti-GFP antibodies (generic goat IgGs as control) or mouse anti-γ-adaptin antibodies (mouse hybridoma C7 antibodies as control) bound to protein G-Sepharose beads (Fisher Scientific). Samples were rotated end-over-end at 4°C for 2 h and washed 2 x in buffer D+ and 1 x in PBS ...
-
bioRxiv - Genetics 2021Quote: ... incubated with mouse anti-HA antibody and then with the secondary antibody conjugated with Donkey-anti-mouse Alexa 567 (Invitrogen Molecular Probes). DNA was stained with 0.5 mg/ml DAPI (Sigma-Aldrich) ...
-
bioRxiv - Molecular Biology 2019Quote: ... quickly washed with PBS+ before incubating with 70 µl of secondary antibody mix (Goat anti-mouse Alexa Fluor® 488 or Goat anti-mouse Alexa Fluor® 594 1/400 in PBS+, Invitrogen) for 1 h at RT in a moist chamber ...
-
bioRxiv - Developmental Biology 2020Quote: ... Pros (mouse 1:100, DSHB MR1A), Hairless (guinea pig 1:500, Annett and Dieter) and V5 (mouse 1:500, Invitrogen R960-25).
-
bioRxiv - Neuroscience 2019Quote: ... 1:200) follwed by Alexa Fluor 488 anti-mouse IgG or Alexa Fluor 568 anti-mouse IgG (A11001 and A11004, Thermo Fisher Scientific) as the secondary antibodies (1:1000) ...
-
bioRxiv - Neuroscience 2020Quote: ... goat anti-mouse IgG1 Alexa Fluor 568, A-21124, or with goat anti-mouse IgG2a Alexa Fluor 488, A-21131, Thermo Fisher Sci) at room temperature (23 °C ...
-
bioRxiv - Physiology 2022Quote: ... Mouse cytokines were measured using a Cytokine & Chemokine Convenience 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen, EPXR260-26088-90, Carlsbad, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... Neutrophils were labelled with 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) 15 min prior to recordings ...
-
bioRxiv - Microbiology 2021Quote: ... 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G ...
-
bioRxiv - Microbiology 2021Quote: ... the murine vasculature was labelled with 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and the human vasculature with 100μg of Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
-
bioRxiv - Microbiology 2021Quote: ... and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) were injected to label and visualize the human vessels ...
-
bioRxiv - Developmental Biology 2022Quote: ... followed by overnight incubation with primary antibody overnight at 4 °C (1:100 rabbit-anti-HNF4A Cell signaling 3113S; 1:250 mouse anti-AFP antibody, Sigma A8452; 1:100 mouse anti CYP3A4 antibody, Life Technologies MA5-17064 ...
-
bioRxiv - Cancer Biology 2022Quote: ... Mouse cytokines were measured using a Cytokine & Chemokine Convenience 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen, EPXR260-26088-90, Carlsbad, CA) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2020Quote: ... incubated with an anti-mouse antibody produced in goat and labeled with a goat anti-mouse antibody at 1:200 (Molecular Probes, tetramethylrhodamine, Invitrogen, T2762) for 45 min at 37°C ...
-
bioRxiv - Developmental Biology 2020Quote: ... an anti-Flag antibody or normal mouse IgG were added to the nuclear extract together with Dynabeads bearing anti-Mouse IgG (Thermo Fisher Scientific). After incubation for 1 h with gradual rotation at 4°C ...
-
bioRxiv - Immunology 2021Quote: The total IgG concentration in mouse serum was determined using the IgG (Total) Mouse Uncoated ELISA Kit (Invitrogen, Cat no. 88-50400) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2022Quote: ... coverslips were incubated for 2 hours in a 1:1000 dilution of secondary antibody (anti-rabbit Alexa 647, Invitrogen, A-27040; anti-mouse Alexa 647, Invitrogen, A-32728, anti-mouse Alexa 488, Invitrogen, A-32723). Coverslips were washed 3 times with blocking buffer ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were incubated overnight at 4 °C with primary antibodies — targeting α-syn (Mouse anti α-syn, AA 124-134, ab1903, Abcam; Mouse anti α-syn 4B12, AA 103-108, IgG1, MA1-90346, ThermoFisher; pericytes (Rabbit anti-PDGFR β ...
-
bioRxiv - Neuroscience 2022Quote: ... donkey anti-mouse IgG, 1:500, 715-165-150, Jackson ImmunoResearch; AF488: donkey anti-mouse IgG, 1:500, A-21202, Life Technologies).
-
bioRxiv - Synthetic Biology 2024Quote: ... as previously described [63] and neutrophils were labelled by were labeled with 2.5 µg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530). Mice were infected by intravenous injection of 100 µl of the bacterial inoculum (107 CFU total ...
-
bioRxiv - Molecular Biology 2024Quote: ... AlexaFluor 488 goat anti-rabbit IgG and AlexaFluor 546 goat anti-mouse IgG or Goat Anti-Mouse IgG (H + L) DyLight 680 Conjugated (Invitrogen REF 35518), Goat anti-Rabbit IgG (H&L ...
-
bioRxiv - Developmental Biology 2023Quote: ... The secondary antibodies used were goat anti-mouse IgG2b 594 and goat anti-mouse IgG1 647 (all from Molecular Probes, 1:500). After several washes in PBS-T ...
-
bioRxiv - Cell Biology 2023Quote: ... and then incubated with the indicated primary antibodies (mouse anti- MIC60 [110329; Abcam], rabbit anti-MIC27 [PA5-51427; Thermo Fisher Scientific], mouse anti- TIMM23 [BDB611222; Thermo Fisher Scientific] ...
-
bioRxiv - Microbiology 2023Quote: ... Monolayers were fixed and stained at 24 hpi with mouse monoclonal antibody 1E12 followed by anti-mouse Alexa Fluor 488 (Thermo Fisher Scientific) and Hoechst nuclear stain ...
-
bioRxiv - Synthetic Biology 2023Quote: The determination of serum TNF-α and IFN-γ concentration was performed using Mouse IFN gamma Uncoated ELISA and Mouse TNF alpha Uncoated ELISA (Invitrogen). The protocols recommended by the vendor were strictly adhered to.
-
bioRxiv - Immunology 2023Quote: ... and stained with a mouse serum that recognizes A27 (1:200 dilution) followed by adding anti-mouse AF488 (Invitrogen, A55058, 1:1000) as secondary antibody ...
-
bioRxiv - Immunology 2024Quote: Mouse lung homogenates were collected for Luminex analysis using the Cytokine & Chemokine 36-Plex Mouse ProcartaPlexTM Panel 1A (ThermoFisher, # EPX360-26092-901). Samples were incubated with fluorescent-coded magnetic beads pre-coated with respective antibodies in a black 96-well clear-bottom plate overnight at 4 °C ...
-
bioRxiv - Cell Biology 2024Quote: ... abdominal carcasses were incubated in AlexaFluor conjugated secondary antibodies (goat anti-mouse 488, goat anti-mouse 568, or goat anti-rat 488, ThermoFisher; 1:250) for two hours at room temperature and protected from light.
-
bioRxiv - Neuroscience 2024Quote: ... gout anti-mouse IgG1 Alexa Fluor 568, A-21124 and goat anti-mouse IgG2a Alexa Fluor 488, A-21131, Thermo Fisher Sci) at room temperature (23 °C ...
-
bioRxiv - Genetics 2024Quote: ... followed by transfected with plasmids expressing mCherry-tagged mouse DNAH12 and GFP-tagged mouse DNALI1 using Lipofectamine 3000 (Thermo Fisher, CA, USA) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: ... anti-rabbit HRP antibody (1:5000) or with anti-goat HRP antibody (1:5000) was performed for 1 h and ECL (Pierce, Thermo Scientific, Rockford, IL, USA) visualized detection ...
-
bioRxiv - Bioengineering 2019Quote: ... for 30 minutes non-shaking to allow for infection and then grown for 3 hours at 37°C and 225 rpm for amplification (MaxQ 4000, ThermoFisher Scientific, Hannover Park, IL). After ...
-
bioRxiv - Physiology 2020Quote: ... Protein concentrations were determined using a BCA kit according to the manufacturer’s protocol (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific, Rockford, IL, USA). Citrate synthase (CS ...
-
bioRxiv - Pathology 2020Quote: ... Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis of interleukin (IL)-6 was performed using TaqMan Gene Expression Assays (Rn01410330_m1; Applied Biosystems, Foster City, CA, USA) and 7900HT Fast Real-Time PCR System (Applied Biosystems) ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... 1 μg of the total RNA of each sample was reverse-transcribed into cDNA using SuperScript III cDNA Synthesis Kit (Thermo Scientific, Rockford, IL, USA). Real-time PCRs were performed using the TaqMan™ Universal Master Mix (Thermo Fisher scientific ...
-
bioRxiv - Neuroscience 2019Quote: 3 × 104 A4 and untransfected SH-SY5Y cells were seeded into 8-well Lab-Tek®II Chamber Slides (Nalge Nunc, Naperville, IL, U.S.A) and left for 48 h to strongly adhere ...
-
bioRxiv - Molecular Biology 2021Quote: ... The bound antibodies were detected by incubation with Alexa Fluor 488-conjugated donkey anti-rabbit IgG (Thermo Fisher Scientific, Rockford, IL, USA; 1:300) or Alexa Fluor 546-conjugated donkey anti-goat IgG (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: In vitro transcription of 230-bp CDS sequences that included nucleotide 402 of WT and 402C>G FOXL2 mRNA was performed using the MegashortScriptTM T7 Kit (Thermo Scientific, Rockford, IL, USA). To generate the T7-FOXL2 DNA template ...
-
bioRxiv - Physiology 2020Quote: ... Supernatant was collected after 15-min at centrifugation (14K x G) and analyzed for protein concentration using Bicinchoninic Acid kit (Thermo Fisher Scientific, #PI208609, IL,).
-
bioRxiv - Microbiology 2020Quote: ... A total of 100 μg protein from each condition was subjected to in-solution trypsin digestion before labelling the resultant tryptic peptides using the TMT-6plex Isobaric Label Reagent Set (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2022Quote: ... The SKBR3 cell-membrane proteins were isolated by either the biotinylation of amino (EZ-Link Sulfo-NHS-SS-Biotin, Thermo Fisher Scientific, Rockford, IL) or oxidized glycan (EZ-Link Alkoxamine-PEG4-Biotin ...
-
bioRxiv - Microbiology 2022Quote: ... Thin sections were cut with a Leica UC7 ultramicrotome (Buffalo Grove,IL) prior to viewing at 120 kV on a FEI BT Tecnai transmission electron microscope (Thermo fisher/FEI, Hillsboro, OR). Digital images were acquired with a Gatan Rio camera (Gatan ...
-
bioRxiv - Neuroscience 2021Quote: ... Protein concentration in the resulting tissue lysate supernatants was measured using the Pierce™ BCA assay kit (Cat. No. 23227, Thermo Scientific, Rockford, IL), and samples were aliquoted and stored at −80°C until analysis.
-
bioRxiv - Immunology 2022Quote: ... Macrophages were cultured in complete RPMI media (cRMPI; RPMI 1650, L-glutamine, 10% heat-inactivated fetal bovine serum (FBS; Gibco, Thermo Fisher Scientific, Rockford, IL), 1% penicillin/streptomycin ...
-
bioRxiv - Developmental Biology 2019Quote: ... The silicon device surfaces were functionalized with a previously described protocol using 3-mercaptopropyltrimethoxysilane (MPTMS) and N-gamma-maleimidobutyryloxysuccinimide ester (GMBS) NeutrAvidin-biotin chemistry (Thermo Fisher Scientific, Rockford, IL)[32 ...
-
bioRxiv - Microbiology 2020Quote: ... followed by incubation with rabbit antibodies against SFTSV-N as primary antibodies and HRP-conjugated recombinant protein A/G (Cat. No. 32490, Thermo Scientific, Rockford, IL, USA) as secondary antibodies ...
-
bioRxiv - Neuroscience 2019Quote: ... and incubated at 37 °C for 7 days with secondary Alexa Fluor 647 donkey anti-rabbit IgG (1:450; Thermo Fisher Scientific, Rockford, IL) in 3% donkey serum/PTwH and then washed in PTwH for 5 times.