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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... FLAG-YB-1 and EGFP-YB-1 (S1 File) in human cells were synthesized and subcloned in the vector pcDNA3.1(+) at GeneArt (Thermo Fisher Scientific). FLAG-M2-2 gene was inserted between the NcoI and AflII restriction sites ...
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bioRxiv - Microbiology 2023Quote: The human type II lung epithelial cell line A549 (ATCC CCL-185) was cultured in DMEM/F12 with GlutaMAX (Life Technologies) supplemented with 10% FBS (FBS ...
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bioRxiv - Bioengineering 2023Quote: Human cervical carcinoma cell line HeLa was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units/ml Pen-Strep (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: Human cervical carcinoma cell line HeLa was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units/ml Pen-Strep (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: Human kidney epithelial cell line HEK293T was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units/ml Pen-Strep (GIBCO ...
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bioRxiv - Biophysics 2023Quote: ... inducible Flp-In T-Rex-293 cell line expressing human RyR2 was generated using the Flp-In T-REx Core Kit (Invitrogen, USA) as described before (54) ...
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bioRxiv - Microbiology 2023Quote: The African green monkey kidney Vero E6 (ATCC, no. CRL-1586) and human Calu3 cells (ATCC, no. HTB-55) were grown in DMEM medium (Thermo Fisher) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2023Quote: ... purified CD4+CD25+CD127lo naive Treg cells (5 × 104) were stimulated with Dynabeads Human T-activator CD3/CD28 (12.5 μl/ml) (Thermo Fisher Scientific) and IL-2 (100 U/ml ...
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bioRxiv - Cell Biology 2023Quote: ... These constructs were then used for in vitro translation using a HeLa cell lysate-based Kit (1-Step Human Coupled IVT Kit—DNA, 88881, Life Technologies). The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: Human placental–epithelial choriocarcinoma cells (JEG-3 HTB-36TM ATCC®) were cultured in Earle’s Minimum Essential Medium (EMEM) (Gibco™) containing 2-mM L-glutamine supplemented with 1% nonessential amino acids (Gibco™) ...
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bioRxiv - Cell Biology 2023Quote: Mouse embryonic fibroblast cells (MEFs) and Human embryonic kidney 293T (HEK293T) were cultured in Dulbecco’s modified Eagle medium (DMEM) (Thermo Fisher Scientific) supplemented with 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Developmental Biology 2023Quote: Stem cells were grown on culture plates coated with 5micro μg ml-1 recombinant human Vitronectin (rhVTN-N, Life Technologies, #A14700). HESCs were grown in mTeSR1 (StemCell Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Hela and 293T cells (Human Embryonic Kidney, HEK293T) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (GIBCO, Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum ...
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bioRxiv - Molecular Biology 2023Quote: ... Hela and 293T cells (Human Embryonic Kidney, HEK293T) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (GIBCO, Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: The human alveolar epithelial cell line A549 (ATCC CCL-185) was cultured in F-12K culture medium (Gibco™ 21127-030) supplemented with 10% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2023Quote: Human ileocecal adenocarcinoma cells (HCT-8; ATCC CCL-244) were cultured in RPMI 1640 ATCC Modification medium (Thermo Fisher A14091-01) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Bioengineering 2023Quote: Human kidney epithelial cell line HEK293T was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units/ml Pen-Strep (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: Human lung Carcinoma cell line A549 was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units /ml Pen-Strep (GIBCO ...
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bioRxiv - Bioengineering 2023Quote: Human lung Carcinoma cell line A549 was maintained in DMEM (HyClone, Cytiva) plus 10% fetal bovine serum (GIBCO, Thermo Fisher Scientific), and 100 Units /ml Pen-Strep (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: The surface of all cultureware used for the maintenance and propagation of human pluripotent stem cells (WTC-11 line) was coated with Corning Matrigel (Fisher Scientific) prior to cell seeding ...
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bioRxiv - Genetics 2023Quote: ... 293T human embryonic kidney epithelial cells (ATCC, #CRL-3216) were grown in complete Dulbecco’s Modified Eagle’s Medium (DMEM) (Thermo Fisher, #11995-065) supplemented with 10% FBS ...
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bioRxiv - Immunology 2023Quote: ... Plasmids encoding paired Ig heavy and light chains were co-transfected into human embryonic kidney (HEK) 293F cells (Thermo Fisher Scientific) for recombinant mAb production ...
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bioRxiv - Immunology 2023Quote: ... Serum starved PANC-1 and MiaPaCa-2 cells were incubated with human properdin (20 µg/ml) and CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (5 μM ...
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bioRxiv - Immunology 2023Quote: Polyclonal T cells were isolated from PBMCs (see above) and pre-activated with DynabeadsTM human T-Activator CD3/CD28 beads (Gibco, #11131D) at a 1:1 ratio of cells:beads with 100 IU/ml IL-2 (Chiron) ...
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bioRxiv - Cell Biology 2023Quote: MRC5 fibroblasts (ATCC cat. no. CCL-171) and adult human mesenchymal cells were cultured in DMEM (cat. no. 11965092, Thermo Fisher) with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... on semiconfluent monolayers of OP9 stromal cells expressing the human Jag2 Notch ligand (OP9-Jag2) (60) and cultured in p24-well plates with α-MEM medium (Gibco) supplemented with 20% FBS ...
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Neuroscience 2024Quote: ... Additionally, human embryonic kidney HEK293T/17 cells (ATCC, Manassas, VA, USA, Cat# CRL-11268) were grown in DMEM media (Thermo Fisher Scientific ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: The human neuroblastoma cell line (SH-SY5Y: ATCC, CRL-2266) was grown in low glucose DMEM medium (11885084, Thermo Fisher Scientific) with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... The ORF of red fluorescent protein gene (RFP)-fused Antp was inserted into a pIZ/V5-His vector (Invitrogen) driven by the OpIE2 promoter ...
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bioRxiv - Molecular Biology 2021Quote: ... and the C-terminal foldon trimerization motif followed by an 8×His-tag was cloned into the pcDNA3.1(+) expression vector (Invitrogen). Furthermore ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder, Applied Biosystems, in 10 μL of Hi-Di formamide ...
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bioRxiv - Biochemistry 2020Quote: ... the magnetic beads were resuspended in 10.0625 μL ROX/Hi-Di (0.0625 μL of ROX 350 ladder [Applied Biosystems] in 10 μL of Hi-Di formamide [Applied Biosystems] ...
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bioRxiv - Biochemistry 2020Quote: ... Binding of the full-length S protein was detected by mouse anti-His IgG (Invitrogen MA121315, 2 μg/ml) and Alexa Fluor-488-labelled goat anti-mouse IgG (Invitrogen A11001 ...
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bioRxiv - Biochemistry 2020Quote: ... Purified PCR products were digested with XhoI and NheI and inserted into the pcDNA6/V5-His expression vector (Invitrogen) generating plasmid pMBA40 for POMGNT1 complementation ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA libraries were pooled for emulsion PCR using an Ion PI Hi-Q Chef Kit (Thermo Fisher Scientific). The enriched samples were loaded onto an Ion PI chip v3 with Ion Chef ...
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bioRxiv - Genomics 2021Quote: ... Sequencing was performed on the Ion Torrent PGM with the Ion PGM Hi-Q View Sequencing kit (Life Technologies) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... the RNA-Seq templates were prepared using the Ion PGM Hi-Q View OT2 Kit (Thermo Fisher Scientific Inc.) on an Ion OneTouch 2 system ...
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bioRxiv - Immunology 2022Quote: ... two alpacas were five times immunized with 200 µg His-NLRP1PYD using Imject™ Alum Adjuvant (Thermo Fisher Scientific) according to locally authorized protocols ...
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bioRxiv - Immunology 2022Quote: ... The plate was coated with 2 µg/mL mouse anti-His antibody (Invitrogen cat. #MA1-21315-1MG, ThermoFisher Scientific) overnight at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... fused with an N-terminal 6×His-tag and cloned into expression vector pPIC9 (Thermo Fisher Scientific, California, USA). Correctness of the resulting constructs was confirmed by DNA sequencing prior to introduction into Pichia pastoris strain GS115 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Protein expression was confirmed by western blot using a 6x His-Tag HRP conjugated Monoclonal Antibody (Thermo Fisher Scientific). Once verified ...
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bioRxiv - Cell Biology 2021Quote: ... reaction mixes were prepared using 2.5 μg recombinant His-ACBD5 with or without the addition of 0.3 mM ice-cold ATP (Thermo Fisher) and 0.1 ug GST-GSK3β (Abcam) ...
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bioRxiv - Biochemistry 2021Quote: ... the membranes were incubated 1 mg L-1 of anti-6X-His tag monoclonal antibody [HIS.H8] with an HRP conjugate (ThermoFisher) suspended in 10 mL 1X TBST for 0.5 hours ...
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bioRxiv - Biophysics 2021Quote: Detergent extracted His-MBP-tagged proteins were applied to a 40 mL POROS MC 20 Ni2+ column (Applied Biosystems) equilibrated with buffer A (200 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate including his-FAP-interacting peptides were collected and submit to a MS facility (Thermo Fisher, Inc.; USA) for analysis ...
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bioRxiv - Biochemistry 2020Quote: pcDNA4-V5-NAA80-M23L was constructed by subcloning NAA80 from pcDNA3.1-NAA80-V525 into the TOPO TA vector pcDNA 4/Xpress-His (Invitrogen). Then the M23L mutation was introduced and the N-terminal Xpress tag was replaced with a V5 tag ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 228-10481) cDNA was cloned into the BamHI-NotI of the pcDNA3.1(+)-myc-His expression vector (Invitrogen, CAT# V80020) yielding pcDNA3.1-BRCA2T (BRCA2/2662T) ...
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bioRxiv - Molecular Biology 2019Quote: ... The first round PCR reaction was performed by adding 1 µl of cDNA template to a 20 µl reaction containing 0.005 U of Platinum Taq Hi-Fidelity polymerase (Invitrogen) as previously described (59) ...