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Citations for Thermo Fisher :
4001 - 4050 of 10000+ citations for Killer cell immunoglobulin like receptor 2DL3 KIR2DL3 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... 20ng/mL recombinant human FGF-basic (Fisher Scientific). For each patient sample ...
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bioRxiv - Cancer Biology 2024Quote: ... human EGF (Thermo Fisher Scientific, Cat. No. PHG6045) and Pen/Strep/Glutamine (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... anti-cGAS antibody (human or mouse-specific, Invitrogen). PLA signals were visualized using a Zeiss LSM 980 Airyscan 2 NLO Two-Photon Confocal Microscope and indicated by PLA red dots per cell.
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bioRxiv - Immunology 2024Quote: ... Monoclonal antibodies targeting Human IgA (Invitrogen, MA5-32575), IgG (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 ng/mL Human Recombinant EGF (Gibco) following manufacturer instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and OxPhos Human WB antibody cocktail (Thermofisher 458199). For visualization ...
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bioRxiv - Cancer Biology 2024Quote: ... human bFGF (Thermo Fisher Scientific, Cat. No. PHG0261), human EGF (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 20ng/mL recombinant human FGF-basic (Fisher Scientific)] ...
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bioRxiv - Immunology 2024Quote: ... followed by globin reduction using GlobinClear Human (ThermoFisher). To generate sequencing libraries ...
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bioRxiv - Neuroscience 2024Quote: ... and a panel of ProcartaPlex Human Simplex (Invitrogen) beads ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 ng/mL human basic FGF (Gibco, PMG0035) and 25 ng/mL FGF10 (R&D ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for gene expression analysis of known estrogen receptor target genes using a QuantStudio™ 3 Real-Time PCR System (Applied Biosystems™, CAT: A28567). All primers were purchased from Integrated DNA Technologies ...
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bioRxiv - Cell Biology 2020Quote: The pCS2 Lrp6-APEX2-IRES-GFP-PAC plasmid was transfected into Human Embryonic Kidney 293T (HEK293T) cells using Lipofectamine2000 (Thermo Fisher Sci.). 48 hours after transfection puromycin was added to the culture medium ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... elegans γ-tubulin were cloned into human expression vectors driven by the CMV promoter (Table 3) and co-transfected into FreeStyle 293-F cells (Thermo Fisher Scientific). The empty 5Myc plasmid (CS2P ...
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bioRxiv - Developmental Biology 2020Quote: ... samples from pools of 20 medaka hatchlings (stage 40) and human fibroblasts (7×105 cells) were lysed with RIPA Lysis and Extraction Buffer (Thermo Scientific, #89900). Samples were purified with methanol/chloroform protein extraction protocol according to (Wessel & Flügge ...
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bioRxiv - Molecular Biology 2020Quote: The dual pri-miRNA expression plasmids were transfected to human embryonic kidney (HEK) 293 cells in 6-well plates using the Lipofectamine 3000 reagents (Thermo Fisher Scientific) following standard procedures ...
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bioRxiv - Genomics 2019Quote: Plasmids were transfected into the human embryonic kidney 293 cell line (HEK293T; ATCC CRL-3216) and grown in DMEM media (Gibco, 10566-016), supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2019Quote: ... iPSCs were cultured on a feeder layer of irradiated mouse embryo fibroblasts using human embryonic stem cell media containing DMEM with F12 (DMEM/F12, 1:1 ratio, Thermo Fisher Scientific) supplemented with 20% Knockout Serum Replacer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2019Quote: ... T-cells were isolated 5 days prior to addition to MCF7 cells and were activated with Dynabeads® Human T-Activator CD3/CD28 (Thermofisher, 11131D) and 2.5 ng/mL of recombinant human IL-2 (Thermofisher ...
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bioRxiv - Cell Biology 2021Quote: ... and human lung carcinoma cell line A549 (ATCC CCL-185) were cultured in the presence of exosome-depleted FBS (Thermo Fisher Scientific) prior to collection of conditioned medium ...
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bioRxiv - Bioengineering 2020Quote: ... Primary human T cells were isolated from PBMCs using Pan T Cell Isolation Kit (Miltenyi, 130-096-535) and activated with Dynabeads® Human T-Expander CD3/CD28 (Gibco, 11141D). Three days later ...
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bioRxiv - Neuroscience 2020Quote: ... HCV-29 human derived urothelial cells were obtained as a gift from Paul Brindley and grown in MEM (Thermofisher Scientific, Waltham, MA) with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2021Quote: The DNA sequence of monomeric GFP was fused at the C-terminal of all human LI-cadherin constructs of which stable cell lines were established and was cloned in pcDNA™5/FRT vector (ThermoFisher Scientific). CHO cells stably expressing LI-cadherin-GFP were established using Flp-In™-CHO Cell Line following the manufacturer’s protocol (ThermoFisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... The cells were treated for 30 minutes with a 100 µL solution containing a mouse-anti-human ASGPR1 antibody (Thermofisher, Waltham, MA) diluted 1:50 ...
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bioRxiv - Bioengineering 2021Quote: ... human induced pluripotent stem (iPS) cells cultured on Matrigel-coated plates were dissociated with warm enzyme-free dissociation buffer (Gibco, 13150-016) and centrifuged twice at 200xg for 5 min each in advanced DMEM/F12 (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Calu-3 (human, Caucasian, lung, adenocarcinoma) cell line was obtained from ATCC and maintained in Minimum Essential Medium (MEM; Gibco, Thermo-Fisher) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2021Quote: ... Human iPS cells were maintained and propagated as colonies in feeder-free conditions in Essential 8 Flex Medium (Thermo Fisher Scientific, A2858501) on Matrigel hES qualified (Corning ...
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bioRxiv - Microbiology 2020Quote: ... To starve Chlamydia of tryptophan HeLa cells were incubated for 24 h in media containing 2 ng/ml recombinant human IFN-γ (Invitrogen: PHC4033) prior to infection ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were pelleted and incubated with 100 μl of human AlexaFlour-488-conjugated antibody (1:200 in PBS/BSA; Thermo Fisher Scientific) and rotated again as described above ...
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bioRxiv - Biophysics 2020Quote: ... while hNAA25 contained a Tobacco-etch virus (TEV)-cleavable N-terminal 6xHis-tag. Human NatB complex (hNAA201-163/hNAA25FL) was obtained by coexpressing these two plasmids in Sf9 (S. frugiperda) cells (ThermoFisher, cat# 12659017), and purified as described previously[35] ...
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bioRxiv - Cancer Biology 2021Quote: ... and metastasis human colon cancer cell line (SW-620) was cultured in Roswell Park Memorial Institute medium 1640 (RPMI 1640: Gibco, Thermo Fisher, USA). All media were supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... For cytokine measurements the cells were plated in the presence of anti-CD3/CD28 (Dynabeads® Human T-activator CD3/CD28, ThermoFisher Scientific) at a 1:5 ratio (bead:cell ...
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bioRxiv - Microbiology 2022Quote: ... Cell-free plasma was analyzed using a human magnetic cytokine panel providing simultaneous measurement of 35 cytokines (Thermo Fisher Scientific, Waltham, MA). The assay was performed according to the manufacturer’s instructions with each subject sample performed in duplicate and then analyzed on a Luminex FLEXMAP 3D instrument.
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bioRxiv - Bioengineering 2022Quote: ... WBCs were isolated from human whole blood and also stained with a live cell marker (10 μM CMTPX, Invitrogen™, Carlsbad, USA). Detailed methods for MCF-7 cell culture and staining of cancer cells and WBCs are provided in our previous works 24-26 ...
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bioRxiv - Bioengineering 2022Quote: ... Human bone marrow mesenchymal stem cells (hMSCs, RoosterBio, Maryland, USA) were cultured in alpha Minimal Eagle’s medium (α-MEM) (Gibco, Carlsbad, USA) with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cell Biology 2022Quote: Male mouse neuroblastoma Neuro-2a (N2a; ATCC, CCL-131) and female human epithelial HeLa (ATCC, CCL-2) cells were maintained in MEM (Thermo Fisher Scientific), supplemented with 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed once and incubated with a PE-labelled goat anti-human IgG secondary antibody (Thermo Fisher Scientific 12-4998-92) at a final concentration of 2.5μg/mL of 30 minutes at 4°C ...
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bioRxiv - Cell Biology 2022Quote: MRC5 cells (human lung fibroblasts, ATCC® Cat# CCL-171TM, RRID:CVCL 0440) were maintained in MEM media (Cat# 11095080, Thermo Fisher Scientific) supplemented with 10% fetal bovine serum ...
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bioRxiv - Immunology 2021Quote: ... and the corresponding light chain (human IgKappa, IgLambda) expression vectors respectively and produced in transiently expressed in Expi-CHO-S cells (Thermo Fisher, #A29133) at 37°C and 8% CO2 ...
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bioRxiv - Microbiology 2021Quote: To facilitate the analyses of invasion assays, HeLa cells (human cervical carcinoma, ATCC® CCL-2™) were cultured in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher) supplemented with 10% (v/v ...
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bioRxiv - Immunology 2020Quote: Human peripheral blood mononuclear cells (PBMCs) from SARS-CoV-2 convalescent donors were stained with Live/Dead Fixable Aqua (Invitrogen; Thermo Scientific) in 100 μL final volume diluted 1:500 at room temperature (RT) ...
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bioRxiv - Biochemistry 2021Quote: ... and the corresponding light chain (human IgKappa, IgLambda) expression vectors respectively and produced in transiently transfected ExpiCHO-S cells (Thermo Fisher, #A29133) at 37°C and 8% CO2 ...
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bioRxiv - Microbiology 2022Quote: His-tagged soluble human ACE2 was produced in FreeStyle™ 293-F cells by transfecting soluble human ACE2 (1-741 aa) expression vector plasmid DNA using 293fectin (Thermo Fisher) and purified using HiTrap Chelating column charged with nickel (GE healthcare ...
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bioRxiv - Microbiology 2022Quote: APEX2 coding sequence (56) with the FLAG epitope at the N-terminus optimized for expression in human cells was synthesized by Invitrogen (GeneArt service). For the transient expression ...
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bioRxiv - Neuroscience 2022Quote: ... were placed in co-culture with astrocytes 24 h following purification and both cell types were maintained in human ESFM supplemented with B-27 (Thermo Fisher Scientific). Co-cultures were maintained for 24 h prior to experiments.
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bioRxiv - Immunology 2022Quote: ... cells were incubated 20 min at 4°C with AF647-conjugated goat anti-human IgG antibodies (1:1000 dilution; Thermo Fisher Scientific) and LIVE/DEAD Fixable Viability dye Aqua (1:1000 dilution ...
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bioRxiv - Immunology 2022Quote: FLAG-DNAM-1-expressing human Treg cells were co-cultured in complete RPMI-1640 culture medium with 2 × 104 CellTrace Violet (Thermo Fisher Scientific)-labeled Tconv cells (freshly isolated from the same donor as that of the Treg cells ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with human PBMCs isolated from healthy donors that were fluorescently labeled with Cell Trace Violet (Invitrogen, Cat. Nr.: C34557) at an effector:target ratio of 20:1 ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... as well as cell culture supernatants were analyzed on a Luminex™ MAGPIX™ instrument with a custom human cytokine panel (ThermoFisher). Samples were thawed at room temperature and prepared according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... or three helper plasmids (pCMV-VSV-G, pMDLg/pRRE, pRSV-Rev) in human embryonic kidney 293T (HEK293T) cells using Lipofectamine 2000 (Thermo Fisher Scientific) following the instructions of the manufacturer ...