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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... final concentration: 5 µM) and 4 µL of 1 mg/mL Hoechst 33342 dye (1:500 dilution) (Life technologies, Eugene, OR, USA) and resuspended thoroughly using a low binding pipette tip ...
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bioRxiv - Neuroscience 2023Quote: ... 10% Donkey Serum, 1% Triton-100, 1h) followed by primary (overnight, 4°C) and secondary antibodies (1-2h, Jackson ImmunoResearch or ThermoFisher Scientific) incubation ...
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bioRxiv - Cell Biology 2023Quote: ... and 20% sucrose-PBS and then incubated overnight at 4°C in a 1:1 solution of 20% sucrose and OCT compound (Fisher Scientific: #23730571). The next day ...
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bioRxiv - Developmental Biology 2023Quote: ... were mechanically lysed in [100 μl/sample + 10 μl extra] lysis buffer (100 mM NaCl, 50 mM Tris, 4 mM EDTA, 1% NP40, 1 μl/ml SUPERaseIN (Thermo Fisher #AM2694), and 1x cOmplete Protease Inhibitor (Roche #11836153001) ...
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bioRxiv - Genomics 2023Quote: ... 2.0 mM 2-mercaptoethanol and 0.1 mg/mL-1 bovine serum albumin) and then stopped by adding 4× LDS sample buffer (∼3.3μL) (Thermo Fisher Scientific, no. NP0007) to a final concentration of 1× (final volume ∼13.3μL) ...
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bioRxiv - Cancer Biology 2023Quote: ... Staining was performed at 1:200 for 30 minutes prior to fixation overnight in 1% phosphate buffered formalin (Fisher Scientific, SF100-4). Note ...
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bioRxiv - Immunology 2023Quote: NK cells were activated by co-culturing NK cells with irradiated K562 cells expressing membrane bound interleukin-21 and 4-1BBL at 1:1 ratio in RPMI (Gibco, Thermo Fisher) supplemented with 10% FCS (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... Primary antibodies were detected using secondary antibodies (diluted 1:500-1:1000 in PBS/4% BSA) conjugated to Alexa Fluor 568 or 647 (Thermo Fisher Scientific). Cells were counterstained with DAPI and kept in PBS until images were taken with the LSM780 microscope.
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bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
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bioRxiv - Synthetic Biology 2021Quote: ... Regulatory T cells were activated at 1 x 106 cells mL-1 for 2 days in complete XVivo15 supplemented with magnetic Treg Xpander CTS Dynabeads (ThermoFisher) at a 1:1 bead to cell ratio and 500 U mL-1 of IL-2 (UCSF Pharmacy) ...
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bioRxiv - Neuroscience 2019Quote: ... KSR medium was gradually transitioned in 25% increments to neural medium (N2/B27-Neurobasal medium, 2% B27 supplement, 1% N2 supplement, 1% Glutamax, (ThermoFisher)) over an 11-day period ...
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bioRxiv - Cancer Biology 2020Quote: ... cells from the previous steps were barcoded by cotransfecting PB-EF1α-Puro-V8.2 library and Super PiggyBac Transposase plasmid (SBI #PB210PA-1) at a 1:10 molar ratio using Lipofectamine 3000 (Thermofisher). Barcoded cells were puromycin-selected for 7 d ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then incubated for 1 hr with goat anti-rabbit Alexa Fluor 488F(ab’)2 Fragment (1:1000, A11070; Invitrogen) and Alexa Fluor 568 Phallodin (A12380 ...
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bioRxiv - Microbiology 2021Quote: ... the sections were incubated with house-made mouse anti-SARS-CoV-2 nucleocapsid protein serum (1:5000) and HRP465 conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The lung sections from the vector-transduced mouse were used as negative control ...
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bioRxiv - Microbiology 2021Quote: ... in 10% FBS for 1-2 hours followed by incubation with secondary goat anti-rabbit AlexaFluor 594 antibody (1:1000, Invitrogen) in 10% FBS for 30 min ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were passaged at a density of 1:2 to 1:8 after reaching ~80% confluency by detaching with 5 mM EDTA in PBS (Invitrogen 15575-038 diluted in sterile 1X PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Medium was replaced at day 2 with N2B27 medium consisting of 1:1 pre-mixed neurobasal medium (#21103-049) and DMEM/F12 (#21331-020, Gibco), 1% B27 supplement without vitamin A (#12587-010 ...
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bioRxiv - Cancer Biology 2021Quote: 2–2.5×106 cells resuspended in a mix of 1:1 PBS and Geltrex Basement Membrane Mix (Thermo Fisher Scientific) were subcutaneously injected in the right flank of 10–12 weeks old NOD/Scid/gamma (NSG ...
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bioRxiv - Neuroscience 2020Quote: ... After 5 days the spheroids were transferred to Neural differentiation medium (NdM) (Neurobasal, 2% B27, 1% GlutaMAX, 1% P/S (all from Gibco) supplemented with 20 ng/mL of EGF and FGF2 (both PrepoTech ...
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bioRxiv - Bioengineering 2022Quote: ... with 50 µL per injection site of vaccine formulations containing 5 µg of SARS-CoV-2 antigen (RBD-SD1, S-2P, S-PCNA1, or 6RBD-np) gently mixed 1:1 (v/v) with AddaVax (Invitrogen) adjuvant ...
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bioRxiv - Cancer Biology 2022Quote: ... using base medium (1:1 ratio of DMEM/F12 Glutamax/Neurobasal medium [Gibco], 2% B27 supplement without vitamin A [Gibco] ...
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bioRxiv - Developmental Biology 2022Quote: ... WIK 24hpf samples were spiked with 2 μl of 1:100 diluted ERCC spike-in mix 1 (4456740, Thermofisher Scientific) while all other samples were spiked with 2 μl of 1:100 diluted ERCC spike-in mix 2 (4456740) ...
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bioRxiv - Neuroscience 2020Quote: ... and equally distributed in wells containing growth medium (DMEM-F12-glutamax [Gibco], 40 μg/mL insulin [Sigma], 1/200 B-27 supplement [Gibco], 1/100 N-2 supplement [Gibco] ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 2-hour incubation with secondary antibody (1:200 Alexa 555 anti-rabbit [Invitrogen]; 1:200 Alexa 488 anti-chicken [Invitrogen]). Following three additional 10-min washes in PBST ...
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bioRxiv - Genomics 2019Quote: ... 1 μg of total RNA was mixed with 2 μl of 1:100 diluted ERCC RNA spike-in (Life Technologies), then ribosomal (r)RNAs were depleted using the RiboMinus Eukaryote v2 Kit (Life Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... 6-diamino-2-phenylindole (DAPI, 1:25,000) for 1 min and the sections were mounted in ProLong® Gold antifade reagent (Invitrogen).
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bioRxiv - Immunology 2021Quote: ... cells were alternatively pre-activated with IL-2 as above and one of: 1:1 anti-CD3/anti-CD28-coated beads (Gibco Dynabeads Human T-Expander or Human Treg Expander) ...
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bioRxiv - Microbiology 2021Quote: ... then covered with 2 mL overlay medium of 2% Avicel CL-611 (DuPont Pharma Solutions) diluted 1:1 with 2x minimum essential media (Gibco). The cells were then incubated for three days ...
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bioRxiv - Cell Biology 2021Quote: ... was added for 2 days (Day 1 and Day 2 of differentiation) followed by a neural induction period in Neural Induction Media (Advanced DMEM/F12 (1:1, Gibco), 1% non-essential amino acids (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were captured on the magnet and the supernatant now containing the dissociated cells was transferred to a 15 ml Falcon tube containing 10 ml ice cold FACS buffer (DMEM w/o phenol red + 1% P/S, 2% FBS, 1 mM EDTA pH 8, all from Gibco) and centrifuged for 5 minutes at 300 g and 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A total of 2 x 106 cells (ratio 1:1) were resuspended in a final volume of 100 uL in DMEM (Invitrogen) and injected into the hypodermis of the mouse skin ...
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bioRxiv - Microbiology 2022Quote: ... Mice were sacrificed at 1 dpi and lungs were collected in 1 mL DMEM/2% FBS/1X Antibiotic-Antimycotic (Gibco). Samples were homogenized and debris was clarified at maximum speed for 10 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: Sorted cells were cultured in 2 ml RPMI 5.5 mM glucose (1:1 mixture of Cat. No. 11875093 and Cat. No. 11879020 [Thermo-Fisher Scientific]) supplemented with 20% FCS (v/v) ...
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bioRxiv - Immunology 2024Quote: ... HeLa (CCL-2, ATCC), COS-1 (CRL-1650, ATCC) and L-929 (CCL-1, ATCC) cells were maintained in DMEM (11995073, Gibco) supplemented with 1% Penicillin-Streptomycin (15140122 ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 10% FBS, 1% Non-essential Amino Acids [NEAA; Wako], 55 µM 2-Mercaptoethanol [Gibco]). Mouse embryonic stem (ES ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated in culture media (66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin and glutamine; all from Invitrogen) and 0.66% D-(+)-Glucose (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The base medium was gradually transitioned to N2/B27 medium (Neurobasal medium, 2% B27 supplement, 1% N2 supplement, 1% Glutamax, (ThermoFisher)) in 25% increments ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: ... and re-plated on Matrigel-coated dishes in NPC Medium (DMEM/F12, 2% B27, 1% N2, 1% Pen-strep; all from Gibco) supplemented with 20 ng/mL bFGF (Peprotech) ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated in PBS for 1 hour at room temperature and incubated 2 hours at room temperature with the appropriated Alexa488 (1/400, Invitrogen), Alexa594 (1/400 ...
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bioRxiv - Cell Biology 2023Quote: ... Counter-staining cell nuclei with a DNA-binding dye 4′,6-diamidino-2-phenylindole (DAPI, 1 μg ml−1 in PBS) and F-actin with Alexa Fluor dye conjugated phalloidin (Invitrogen) was also done at this step ...
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bioRxiv - Neuroscience 2022Quote: ... the medium was changed to neural induction media (1:1 mixture of DMEM/F-12 GlutaMAX: Neurobasal media supplemented with 0.5x N-2 (Life Technologies), 0.5x B-27 (Life Technologies) ...
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bioRxiv - Biophysics 2023Quote: ... the purified DNA in 1 ml NaHCO3 (pH 8.3) was incubated with 1 mM SM(PEG)2 (Thermo Scientific Pierce) for 20 mins at 20-22°C ...
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bioRxiv - Cancer Biology 2023Quote: BE(2)-C (ATCC, CRL-2268) cells were cultured in a 1:1 mixture of DMEM/F12 (with HEPES, Gibco) and EMEM (ATCC) ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 Nucleocapsid antibody (produced in mouse) was procured from (MA5-35943/Invitrogen/Massachusetts, USA/1:1000-1:10,000) used as primary antibody ...
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bioRxiv - Microbiology 2023Quote: ... buffer (1× PBS containing 2% FBS) followed by incubation with Alexa Flour 633 goat anti-human IgG (1: 2000 dilution, Invitrogen). Finally ...
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bioRxiv - Developmental Biology 2023Quote: ... The culture medium consisted of a 1:1 mixture of DMEM with 2 mM l-glutamine and F12 (Ham’s Nutrient Mix) (both from Life Technologies) and was supplemented with 10% (vol/vol ...
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bioRxiv - Neuroscience 2023Quote: ... Dissociated cells were suspended in culture medium (Neurobasal medium, 2% B27, 1% GlutaMAX™, GIBCO, 1% antibiotic/antimycotic (ThermoFisher Scientific)) supplemented with mouse NGF (50ng/μl ...
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bioRxiv - Immunology 2023Quote: The whole blood samples were 1:1 diluted with 2% fetal bovine serum (FBS) (Hyclone, SH30406.05) in phosphate buffered saline (PBS) (Invitrogen, C10010500BT) and subjected to Ficoll (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg total RNA was mixed with 2 μl 1:100 diluted ERCC RNA Spike-In Mix (Thermo Fisher Scientific). Libraries were prepared with the NEBNext Ultra II Directional RNA Kit (NEB) ...