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Citations for Thermo Fisher :
3601 - 3650 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Total RNA from SARS-CoV-2 USA-WA1/2020 or rSARS-CoV-2 infected (MOI=0.01) Vero E6 cells (106 cells/well, 6-well plate format) was extracted with TRIzol Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: HEK293F cells grown in a 6-well plate were transiently transfected with CFTR constructs labelled with C-terminal eGFP tag using Lipofectamine 3000 (Thermo Fisher) in Opti-MEM (GIBCO ...
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bioRxiv - Cancer Biology 2021Quote: ... Experiments were conducted by plating cells in a 6 well plate for next day transfection when cells where 70-80% confluent using Lipofectamine RNAiMax (Invitrogen; 13778100). A final concentration of (20 pmoL ...
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bioRxiv - Cell Biology 2021Quote: ... at 1000xg for 15 min followed by 10,000xg for 15 min in 500 ml vessels using a fixed angle FIBERlite F14-6×500 y rotor (Thermo Fisher Scientific). The supernatant was centrifuged at 100,000xg (28,000 RPM ...
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bioRxiv - Microbiology 2020Quote: ... or by incubating at 37°C for 48h and subsequently adding 6 µM Hoechst 33342 nucleic acid stain (Thermo Fisher Scientific) as a background stain for high content imaging analysis ...
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bioRxiv - Cell Biology 2020Quote: ... 0.3×106 control and senescent AML12 cells were seeded in each well of 6-well plate for cell replication analysis and counted (using Countess™ II Automated Cell Counter, ThermoFisher) at every other day for three days as shown in figure 1c.
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bioRxiv - Cell Biology 2019Quote: ... Transient expression was obtained by electroporation performed 24 hours before the experiment using the Neon electroporation system (Thermo Fisher 6 Scientific). For each transfection ...
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bioRxiv - Cell Biology 2019Quote: Analysis was performed using the Applied Biosystems validated TaqMan primer-probe set (Hs00208333/ spans exon 6 and 7) and the ABI PRISM SDS7000 sequence detection system (Applied Biosystems). The ΔCT method was used to quantify relative mRNA levels as described in ABI user guide ...
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bioRxiv - Cell Biology 2020Quote: ... A gradient consisting of 0% to 40% B in A (A: 0.1% formic acid [Fisher Scientific, A117], 6% acetonitrile [Fisher Scientific, A955] ...
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 were measured according to the manufacturer’s instructions in plasma and peritoneal lavage supernatant using Novex ELISA Kits (Thermofisher Scientific Ltd.).
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bioRxiv - Cell Biology 2020Quote: Quantitative PCR was done on the QuantStudio 6 Flex with the QuantStudio Real-Time PCR software using SYBR green PCR master mix (Applied BioSystems). Primers (see Appendix S2 for primer sequences ...
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bioRxiv - Microbiology 2020Quote: ... Excess protein was blotted away for 6 seconds before being plunge frozen into liquid ethane using a Vitrobot Mark IV (Thermo Scientific). Frozen grids were imaged in a Titan Krios (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Primary neurons were plated onto poly-L-lysine-coated coverslips for 6-well plates and cultured in MEM medium (Thermo Fisher) containing 2% B27 and 5% FCS ...
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bioRxiv - Microbiology 2019Quote: ... Plates were washed 6 times and incubated with horseradish peroxidase (HRP)-conjugated mouse anti-human IgG Fc secondary antibody (Cat# 05-4220; ThermoFisher Scientific) diluted 1:1000 in 100 µl blocking buffer for 1 h at room temperature or 37°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... 2×105 cells were plated per well of a 6-well plate and 18 hours later cells in 1750μl of Opti-MEM (31985070, Thermo Fisher Scientific) were transfected with 50nM of siRNA targeting HAND2 (s18133 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A 3’ Venus tag was added by recombining the Nvsix3/6 coding sequence into pSPE3-R-Venus destination vector using the Gateway LR cloning reaction (ThermoFisher Scientific). mRNA synthesized and injected at 300ng/µL using previously described methods 61 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 μL; Thermo Fisher Scientific) in OptiMEM (400 μL ...
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bioRxiv - Cancer Biology 2022Quote: ... Using a 6 to 30% gradient over 84 mins peptides were separated and analyzed using Thermo Fusion Lumos mass spectrometer (Thermo Scientific). Following parameters were used- MS1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Over-expression vectors were prepared by cloning the PLAGL2 coding sequence into BII-ChBtW and IEC6 cells transfected in 6 well plates with 1600 ng of this plasmid and 400 ng of pCMV-hyPBase using Lipofectamine 2000 (ThermoFisher Inc.). Cells were select 48 hours after transfection with medium containing 4 µg/ml Blasticidin and selected for 5 days ...
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bioRxiv - Molecular Biology 2020Quote: ... 4 and 6 were directly cloned into pCR(tm)2.1-TOPO® using the TOPO® TA Cloning® Kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: DR-U2OS and -HCT116 cells and EJ-U2OS and -HCT116 reporter cells were plated in 6 well plates and transfected with 5 μL Lipofectamine RNAiMAX transfection reagent (Thermo Fisher) and siRNAs (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: Approximately 4 x 105 HEK293T cells in a 6-well plate were transfected with 250 ng/well of pMT107-octa-6 His-ubiquitin or 200 ng/well of pcDNA3-2x-6His-ubiquitin constructs using 50 µl/well Opti-MEM (Gibco; 31985062) and 2 µl/well of FuGENE 6 Transfection reagent (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction mixtures containing SYBR Green were composed following the manufacturer’s protocol and then CT values were obtained using a qPCR platform (QuantStudio 6 Flex Real-Time PCR System, ThermoFisher Scientific). Real-time PCR data was monitored using QuantStudio 6 Design and Analysis Software Version 2.3 ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Each cDNA sample was diluted 1:25 and real-time quantitative PCR (qPCR) was performed in duplicate using miRNA-specific primers/probe on a Quant 6 studio Real Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: Approximately 60 WT and MafAS64F/+ islets from at least 6 female and male mice were analyzed with the ratiometric calcium indicator fura-2-acetoxymethylester (Fura-2 AM) (Life Technologies). Islets were maintained in 5 mM glucose for 30 min prior to measuring 11 mM glucose-induced calcium oscillations and depolarization-activated calcium influx with 30 mM KCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Aggregation was initiated separately at a concentration of 6 μM for each construct in vitro by addition of AcTEV™ protease (Invitrogen), as previously described for mEx1-Q51 (Shahmoradian et al. ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 × 105 of the sorted cell types and the unsorted mixture were seeded and cultured in 6-well plates coated with fibronectin bovine (LIFE Technologies). Medium composition was equivalent to the 3D differentiation.
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bioRxiv - Bioengineering 2022Quote: Primary neonatal cardiomyocytes were isolated from C57BL/6 mouse neonates on postnatal days 3-5 using the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher) as previously described (14) ...
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bioRxiv - Cell Biology 2022Quote: ... the tryptic peptides from top and bottom of Stentor cell were labeled isobarically with the 6-plexed tandem mass tag (TMT) reagents (ThermoFisher Scientific): TMT126 (top) ...
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bioRxiv - Immunology 2022Quote: ... BMDMs were seeded into 6-well plates (one million per well) in DMEM with 10% dialyzed FBS (Thermo Scientific, Waltham, MA), 25 mM unlabeled glucose ...
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bioRxiv - Genomics 2020Quote: ... 10 ng of the library were transfected into 250 000 HEK293 cells in one well of a 6-well plate using Lipofectamine 2000 (11668027, ThermoFisher Scientific) and OPTIMEM I Reduced Serum Medium (31985-047 ...
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bioRxiv - Genomics 2020Quote: ... 1.5 million cardiomyocytes were re-plated per well of a 6-well plate on Day 20 in Cardiomyocyte Maintenance Media [500 mL DMEM without glucose (A14430-01, ThermoFisher Scientific), 50 mL FBS (S1200-500 ...
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bioRxiv - Immunology 2021Quote: ... FAM-ACACTAGCCATCCTTACTGCGCTTCG-BHQ) and PCR reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems). To generate a standard curve ...
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bioRxiv - Immunology 2021Quote: ... was added to each sample for the PCR reaction and run on the QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific). The value of STAT3 Exons 20/21 and Introns 20/21 was calculated as 2ΔCt(GAPDH) ...
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bioRxiv - Immunology 2021Quote: ... the barcoded samples were combined and diluted to 6 x 106 cells / 800 μl CyFACS per well in Nunc 96 DeepWellTM polystyrene plates (Thermo Fisher). Cells were blocked with mouse (Thermo Fisher) ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting suspension with all nuclei was stained with the fluorescent DNA intercalant marker DAPI (4’-6-diamidino-2-phenylindole dihydrochloride, Invitrogen, USA) diluted 1:20 from a stock solution of 20 mg/L and filled with PBS 0.1M to known volume ...
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bioRxiv - Zoology 2020Quote: ... Tissues were cut into small pieces with a scalpel and washed twice in ADPBS containing antibiotics prior to placing 4-6 tissue pieces into a 25 cm2 plug-seal tissue culture treated flask (BioLite, Thermo Scientific) containing 1.5 mL establishment medium to cover the tissue pieces ...
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bioRxiv - Molecular Biology 2019Quote: ... All RT products were used in total of 300 ul (50 uL × 6) PCR reaction with Phusion polymerase (Thermo Fisher Scientific) and gene specific primers with Fluidigm mmPCR adaptor sequences51 ...
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bioRxiv - Pathology 2019Quote: ... IL-6 and IL-10 levels were assessed using ELISA kits (Cat# 88-7324-22, BMS603-2, 88-7105-22 Thermofisher, USA). The intensity of the colour was measured using a microplate reader (Thermal ...
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bioRxiv - Genomics 2020Quote: ... and approximately 1.6 × 106 C57BL/6 ESCs were transfected with 15 μg of linearized vector using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2019Quote: ... Differentiation experiments were carried out for 10 days on gelatin-coated 6-well plates by transitioning cells in Dulbecco modified Eagle medium (DMEM, ThermoFisher Scientific) with 10% of fetal bovine serum ...
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bioRxiv - Developmental Biology 2019Quote: ... blastocysts were collected from uterine horns and put on culture for 3-6 days in ES derivation medium composed of GlutaMAX/DMEM (Gibco, 31966), 15% FBS (Biowest) ...
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bioRxiv - Neuroscience 2019Quote: ... The medium was changed every day and the cells were passaged every 5-6 days using PBS without Calcium and Magnesium (Life Technologies).
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bioRxiv - Developmental Biology 2019Quote: ... then transferred into agarose-coated 6-well plates containing explant medium - comprised of Dulbecco’s modified eagle medium with nutrient mixture F-12 (Gibco DMEM/F12) containing 2.5mM L- Glutamine ...
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bioRxiv - Cell Biology 2019Quote: The staining of limiting membrane of the yeast vacuole was achieved with FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Invitrogen). Cells were grown to early-log phase in appropriate medium ...
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bioRxiv - Physiology 2019Quote: ... 5 muscle samples 100 μg each corresponding to 5 different age groups and one separately prepared master reference sample were labeled with 6-plex tandem mass spectrometry tags using a standard TMT labeling protocol (Thermo Fisher). 200 femtomole of bacterial beta-galactosidase digest (SCIEX ...
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bioRxiv - Systems Biology 2019Quote: A mate-pair library with 3-6 kb fragments was prepared from pure genomic DNA using Ion TrueMate Library Reagents (Life Technologies). The Ion PGM™ template OT2 400 kit (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... Differentiation to ECs were initiated by treating the hiPSCs with 6 μM CHIR99021 in RPMI-B27 without insulin media (Life Technologies) for 2 days ...
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bioRxiv - Bioengineering 2019Quote: B-cells were isolated from two C57BL/6 mice via MACS depletion (CD43 microbeads, 130-049-801 MACS) and labelled with Cell Trace Violet (ThermoFisher, C34557) or Cell Trace Far Red (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...