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Citations for Thermo Fisher :
3601 - 3650 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: The rat insulinoma cells (INS-1, 832/3)54 were cultured in RPMI-1640 medium (Thermo Fisher Scientific, 11875119) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transiently transfected with a rat p75NTR-EGFP* fusion constructs31 using FuGENE transfection reagents (Fisher scientific, Cat: PRE2311). EGFP* consist of a monomeric A207K EGFP mutant ...
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bioRxiv - Neuroscience 2022Quote: ... DRG were dissected from adult rats (10-12 months old) (L2-L5) and incubated with 0.5% collagenase (Gibco 17104019) at 37 °C for 1 hr ...
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bioRxiv - Developmental Biology 2022Quote: ... The excised node region was exposed to DMEM supplemented with 75% rat serum and 0.2-µm-diameter fluorescent beads (505/515, F8811; Invitrogen), and the motion of the beads in the node cavity was monitored in planes of ∼10 µm above the surface of node pit cells.
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bioRxiv - Developmental Biology 2020Quote: ... Single-cell suspensions were incubated with a rat anti-CD49f antibody conjugated with phycoerythrin (ThermoFisher Scientific, clone NKI-GoH3), followed by anti-phycoerythrin magnetic microbeads and enriched using a column-magnetic separation procedure (Miltenyi Biotec) ...
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bioRxiv - Microbiology 2021Quote: ... and incubated for 20 minutes with anti-rat IgG H&L labelled with AlexaFluor 568 (10 µg/ml in 0.1% (v/v) horse serum (Gibco), 0.1% (w/v ...
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bioRxiv - Cell Biology 2019Quote: Py-3Y1-S2 rat fibroblast cells were cultured in Dulbecco’s minimum essential medium (DMEM) containing 5% fetal bovine serum (Gibco, Thermo Fisher Scientific K.K. ...
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bioRxiv - Developmental Biology 2020Quote: ... After three 15 minutes washes with PBST (PBS, Tween 20 0.1%) the secondary antibody (HRP-Anti Rat (Life Technologies) 1:4000 was added and incubated for two hours at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Coverslips were washed with 5% FBS and stained with AlexaFluor-488-conjugated goat anti-rat IgG (Invitrogen, 1:500) for 1 h at RT ...
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bioRxiv - Genomics 2021Quote: Total RNA from rat liver samples was extracted according to the manual of mirVana mRNA Isolation reagent (Ambion, USA). The transcriptome sequencing was performed in collaboration with OE Biotech Co. ...
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Modified N-linked glycosylation status predicts trafficking defective human Piezo1 channel mutationsbioRxiv - Biophysics 2020Quote: ... mCherry-fused Piezo1 protein was probed with rat monoclonal anti-mCherry antibody (Clone 16D7, ThermoFisher Scientific; 1:1,000 dilution); the native human Piezo1 channel was probed using a mouse monoclonal anti-Piezo1 antibody (Cat# NBP2-75617 ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibodies were goat anti-rat AF647 and anti-mouse AF488 (diluted 1:1000, ThermoFisher #A-21247 #A-28175). The pan-flavivirus anti-Env 4G2 antibody was a kind gift from Phillipe Desprès.
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Intrarenal B cells integrate in situ innate and adaptive immunity in human renal allograft rejectionbioRxiv - Immunology 2020Quote: ... and incubated with a combination of primary antibodies: rat or rabbit anti-CD19 (Invitrogen, 6OMP31 or abcam, EPR5906 respectively), rabbit anti-AHNAK (Proteintech ...
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bioRxiv - Cell Biology 2021Quote: ... and cells were collected in a 15 mL conical tube with 25uL Dynabeads® sheep anti-Rat IgG (Invitrogen) coated with ICAM-2 (BD Biosciences Pharmingen ...
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4°C and with anti-rat IgG Alex Fluor 488 (ThermoFisher, Cat# A-11006, dilution 1:1000), anti-guinea pig IgG Alex Fluor 568 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... captured dsRNA was detected by incubating with sulfo-tagged rat anti-mouse IgM antibody (Thermo Fisher Scientific, Waltham, MA) for 1h before washing and adding MSD GOLD Read Buffer A (Meso Scale ...
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bioRxiv - Bioengineering 2022Quote: ... Cortices were dissected from rats of either sex and digested with papain (0.5 mg/mL in Earle’s balanced salt solution) (Thermofisher Scientific). Neurons were plated onto poly-D-lysine coated glass bottom culture dishes in Dulbecco’s Modified Eagle Medium (Thermofisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Hippocampal cultures were prepared from E18 Wistar rat embryos and were maintained in Neurobasal medium supplemented with B27 (Invitrogen) for 3 weeks as described (41) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates had been pre-coated with 12.5 μg/cm2 rat-tail collagen type I (Gibco, ThermoFisher Scientific, Perth, UK). Subsequently ...
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bioRxiv - Microbiology 2022Quote: Secondary antibodies used in this study were Alexa Fluor 488 goat anti-rat IgG (1:400, Invitrogen, A-11006), Alexa Fluor 488 goat anti-mouse IgG (1:400 ...
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bioRxiv - Developmental Biology 2024Quote: ... they were rinsed twice in 1X PBS and incubated with 1:1,000 dilutions of Alexa Fluor-conjugated donkey anti-mouse or donkey anti-rat secondary antibodies (Invitrogen) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... overnight at 4 °C followed by detection with Alexa 488 conjugated anti-rat secondary antibody (1:1000, Life Technologies). Arteries were identified by immunostaining with a mouse monoclonal anti actin ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates had been pre-coated with 12.5 μg/cm2 rat-tail collagen type I (Gibco, ThermoFisher Scientific, Perth, UK). Subsequently ...
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bioRxiv - Microbiology 2023Quote: HEK293T/hACE2 cells stably expressing CNP/HA or GFP/HA were seeded in 24-well plates pre-coated with 50µg/mL Rat Tail Collagen I (Gibco), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... VCAM and MCAM were detected by staining with Alexa Fluor 555 (A555) anti-rat IgG (1:200, A11006, Invitrogen). DAPI (Molecular Probes ...
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bioRxiv - Developmental Biology 2024Quote: ... Secondary antibodies used included HRP-conjugated sheep anti-mouse (Amersham) and HRP-conjugated goat anti-rat (Thermo Fisher Scientific). For densitometry analysis of immunoblots ...
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bioRxiv - Immunology 2023Quote: Mice were intraperitoneally (i.p.) injected with 200 μg of rat anti-mouse CD4 monoclonal antibody (Clone GK1.5; ThermoFisher Scientific) 1 day prior to RICs immunization ...
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bioRxiv - Neuroscience 2023Quote: The following secondary antibodies were used: Alexa Fluor 555 goat anti-rat (1:1000, Thermo Fisher Scientific A-11006), Alexa Fluor Plus 555 goat anti-rabbit (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Compensation controls used splenocytes for most cell surface markers and compensation beads (BD Biosciences, 552844 for rat hosts; ThermoFisher, 01-3333-41 for mouse or Armenian hamster hosts ...
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bioRxiv - Biochemistry 2023Quote: ... the cells were incubated with Alexa-488 goat anti-rat antibodies at 1:200 (Thermo Fisher Scientific, Cat#A11006) diluted in DPBS with 2% BSA for 1 h ...
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bioRxiv - Developmental Biology 2023Quote: ... The following secondary antibodies were used: donkey anti-rat Alexa Fluor 488 (1:400, A-21208, Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 555 (1:400 ...
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bioRxiv - Cell Biology 2023Quote: ... Human microglial cells were cultured in cell culture flasks coated with 50 μg/ml rat tail collagen I (Gibco) in high glucose DMEM (4.5 g/l ...
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bioRxiv - Cell Biology 2023Quote: ... Secondary antibodies were used at 1:2,500 for 1 hr at room temperature: Alexa Fluor 488 goat anti-rat (cat# A11006; Invitrogen), Alexa Fluor 594 goat anti-rabbit (cat# A11012 ...
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bioRxiv - Plant Biology 2023Quote: ... The secondary antibody was a goat anti-rat IgG coupled to the fluorescent dye Alexa Fluor 555 (Molecular Probes) and was used at a 1:100 (v/v ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 2.4G2 hybridoma (ATCC hb-197) tissue culture supernatant and Rat IgG isotype control (Invitrogen, #10700) to block non-specific binding via Fc interactions ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-Kcnj10 (Kir4.1) (1:400, APC-035; Alomone labs)rat anti-Lgals3 (1:200, 14-5301-82; ThermoFisher); rabbit anti-Lxn (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Coverslips were incubated in PBS containing both goat anti-rat 647 secondary antibody (1:500; Cat# A-21247, ThermoFisher) and goat anti-mouse 488 secondary antibody (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies (goat-anti-mouse Alexa Fluor 488 and goat-anti-rat Alexa Fluor 555 (both 1:500, Invitrogen) were incubated for 1.5h in blocking solution ...
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bioRxiv - Developmental Biology 2023Quote: ... The embryo with magnetic beads injected was placed into the customized imaging chamber and immobilised by DMEM without phenol red containing 12.5% rat serum and 1% low-melt agarose (Invitrogen). The temperature of the imaging chamber was maintained at 37 °C with 5% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... The cell pellet was resuspended in 2mL medium with sheep-anti-rat IgG Dynabeads™ (Cat#: 110-35, Invitrogen) coupled with a CD31 antibody (clone Mec13.3 ...
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bioRxiv - Cell Biology 2023Quote: ... The plate was washed 3x with PBS followed by secondary incubation with 568 goat anti-rat (Life technologies, A11077) at 1:1000 and Hoechst (1:2000 ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was extracted from the amygdala of adult rats using the MagMAX mirVana Total RNA Isolation kit (ThermoFisher). RNA quality was assessed using the Qubit RNA BR Assay (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... The Treg panel cells were stained with PE anti-mouse/rat Foxp3 (clone FJK-16s, ThermoFisher Scientific, 1:100) for 30 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.3% Triton 1X TBS solution prior to be stained with Rat anti-mouse CD31 ab (Fisher Scientific, DIA-310) overnight at 4C ...
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bioRxiv - Cancer Biology 2023Quote: ... Hs578T were plated embedded in DMEM supplemented with 2 mg/ml collagen type I (rat tail) (Fisher scientific #CB354249) in a 96-well plate with stoppers creating a central cell-free detection zone ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 2.4G2 hybridoma (ATCC hb-197) tissue culture supernatant and Rat IgG isotype control (Invitrogen, #10700) to block non-specific binding via Fc interactions ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 2.4G2 hybridoma (ATCC hb-197) tissue culture supernatant and Rat IgG isotype control (Invitrogen, #10700) to block non-specific binding via Fc interactions ...
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bioRxiv - Neuroscience 2023Quote: ... The concentration and the purity of isolated rat fecal DNA was determined using Nanodrop spectrophotometer (Thermo Fisher Scientific, USA).
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bioRxiv - Cancer Biology 2024Quote: ... and incubated for 2h at RT with secondary antibody mix of Alexa Fluor 647 anti-rat (Invitrogen, A-21247) and Alexa Fluor 488 anti-mouse (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The surface of the gel was then rendered permissive to cell adhesion by coating Rat Tail collagen I (Gibco, Thermo Fisher Scientific ...