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Citations for Thermo Fisher :
3851 - 3900 of 10000+ citations for RFamide Related Peptide 1 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... the RBD was inserted into pcDNA3.4 with a streptavidin binding peptide (SBP) tag at the C-terminus and were produced using the Expi293 Expression System (Thermo Fisher Scientific, Waltham, MA) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were loaded onto a C18 pre-column (3 μm, 75 mm i.d. × 2 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) then separated on a reverse-phase nano-spray column (2 μm ...
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bioRxiv - Neuroscience 2024Quote: LC-MS/MS analysis for DDA Peptides from each pooled fraction were first spiked with iRT standard peptides (Biognosys AG, Switzerland) and separated using reverse-phase high-performance liquid chromatography (RP-HPLC) on EASY-nLC system (Thermo Fisher Scientific, Bremen, Germany) with a column (75 μm × 150 mm ...
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bioRxiv - Cancer Biology 2024Quote: Tandem Mass Tag (TMT)-10 labeling of peptides was conducted as follows: The TMT-10 plex Isobaric Label Reagent (Catalog No. 90110, Thermo Fisher Scientific, Waltham, MA) was dissolved in anhydrous acetonitrile (ACN ...
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bioRxiv - Microbiology 2024Quote: ... followed by a peptide clean-up on SPE spin columns before TMT labeling with the TMTpro™ 16plex Label Reagent Set (Thermo Fisher, Cat# A44520). Peptides from each sample were labeled with a distinct TMTpro™ isobaric tag before mixing ...
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bioRxiv - Bioengineering 2023Quote: ... the peptides were separated and analyzed using a Dionex Ultimate 3000 RSLCnano system coupled to a Q Exactive instrument (Thermo Fisher Scientific, MA, USA). Separation was performed on a Dionex EASY-Spray 75 μm × 10 cm column packed with PepMap C18 3 μm ...
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bioRxiv - Cancer Biology 2024Quote: ... Tryptic peptides extracted from each IP product were separated by reverse-phased nano-flow liquid-chromatography (EASY-nLC II, Thermo Scientific, San Jose, CA) and analyzed on an LTQ/Orbitrap Velos Elite hybrid mass spectrometer (Thermo-Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... The peptide amount of tumor cell samples was estimated using a fluorescence based protein assay (Qubit Protein assay, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2023Quote: ... Eluted peptides were subjected to electrospray ionization and then entered into an LTQ Orbitrap Velos Elite ion-trap mass spectrometer (Thermo Fisher Scientific, Waltham, MA). Peptides were detected ...
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bioRxiv - Cell Biology 2023Quote: Peptide concentrations of whole cell S-trap extracts in 100 mM TEAB were quantified using the Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... each peptide sample (approximately 2 mg) was tapplied to a Pierce High-Select™ TiO2 Phosphopeptide Enrichment Kit (Thermo Fisher Scientific, Cat No: A32993). After preparing spin tips as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... peptides were reconstituted in 0.1% trifluoroacetic acid and fractionated on Sep-Pak® Vac cartridges using methodology and reagents from Pierce™ High pH reversed-phase peptide fractionation kit (8 fractions for global proteomics and 4 for phosphoproteomics skipping every other; Thermo Fisher Cat No: 84868). Samples were run (1/8th of each global and 1/5th of each phosphopeptide fraction ...
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bioRxiv - Cancer Biology 2023Quote: ... the peptides were analyzed by online nanoflow liquid chromatography-tandem mass spectrometry performed on an EASY-nanoLC 1200 system (Thermo Fisher Scientific, MA, USA) connected to a Q Exactive™ Plusmass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and washed for 3 min at 30 μL/min before peptides were eluted onto a C18 Acclaim™ PepMap™ column (100 Å, 5 μm × 0.75 mm × 150 mm, Thermo Fisher Scientific) at a flow rate of 0.3 μL/min ...
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bioRxiv - Biochemistry 2023Quote: ... as three full experimental technical replicates (n=54) with ∼100 ng of peptides injected for each chromatographic run using a Dionex UltiMate 3000 uHPLC system (Thermo Fisher Scientific, Bremen, Germany). Solvent A was 1% CH3CN in 0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: For the identification of the peptides a Dionex Ultimate 3000 RSLCnano System coupled to an Orbitrap Eclipse Tribrid Mass Spectrometer (both Thermo Fisher Scientific, Vienna, Austria) was used.
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bioRxiv - Immunology 2023Quote: The inhibition of kinase activity mediated by phospholipids was determined using the following Z’-LYTE Kinase Assay Kits: Z’-LYTE Kinase Assay Kit - Ser/Thr 6 Peptide (Thermo Fisher Scientific, Cat# PV3179) used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Direct pepscan analysis was undertaken by binding 200 ng of each overlapping peptide to Nunc MaxiSorp 96 well ELISA plates (Thermo Fisher Scientific, Waltham, MA) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were loaded on an Acclaim PepMap 100 μ-precolumn cartridge (5 μm, 100 Å, 300 μm ID x 5 mm, Thermo Fisher Scientific) and separated at 40°C on a PicoTip emitter (noncoated ...
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bioRxiv - Biophysics 2024Quote: ... Degraded ProteaseMAXTM was removed via centrifugation (max. speed, 10 minutes) and the peptide solid phase extracted using 10 µl volume C18 tips (Pierce○R (Thermo Fisher Scientific, Waltham, MA) according to manufacturer protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Peptides were loaded onto a C18 Acclaim PepMap NanoLC column (25 cm length, 75 µm inner diameter, Thermo Fisher Scientific, San Jose, CA, USA) packed with 2 µm particles and a 100 Å pore size ...
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bioRxiv - Developmental Biology 2021Quote: ... peptide samples were purified via Stage tips using punched out cookies of C18 3M™ Empore™ extraction disc material (Fisher Scientific #14-386-2) as matrix ...
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bioRxiv - Cell Biology 2022Quote: Protein identification by LC-MS/MS analysis of peptides was performed using an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo Fisher Scientific, San Jose, CA) interfaced with an Ultimate 3000 Nano-HPLC apparatus (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: Nanoflow LC–MS/MS analysis of tryptic peptides was conducted on a quadrupole Orbitrap mass spectrometer (Q Exactive HF-X, Thermo Fisher Scientific, Rockford, IL, USA) [48] coupled to an EASY nLC 1200 ultra-high-pressure system (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... MS spectra and MS/MS spectra were acquired from eluting peptides ionized with electrospray ionization (ESI) and analyzed in a LTQ Orbitrap Velos hybrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), as described previously (Verberkmoes et al. ...
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bioRxiv - Neuroscience 2020Quote: ... The tryptic peptides were separated and analyzed using a Dionex Ultimate 3000 RSLCnano system coupled to a Q Exactive instrument (Thermo Fisher Scientific, San Jose, CA) as previously described (Gallart-Palau et al. ...
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bioRxiv - Cancer Biology 2021Quote: Peptides from all experiments were separated by nanoscale C18 reverse-phase liquid chromatography using an EASY-nLC II 1200 (Thermo Fisher Scientific, Waltham, MA, USA), using a binary gradient with buffer A (2% acetonitrile ...
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bioRxiv - Molecular Biology 2020Quote: ... The tryptic peptides were loaded at 5 μL/min onto a C18 trap column (Thermo Scientific, 100 µm ID×2 cm, 5 μm, 120 Å). Peptides were separated with PicoFrit columns (New Objective ...
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bioRxiv - Biophysics 2020Quote: ... The eluted peptides were analyzed in a mass spectrometer with an orbitrap mass analyser (Orbitrap Fusion™ mass spectrometer, Thermo Scientific, San Jose, California, USA).
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bioRxiv - Neuroscience 2021Quote: ... Briefly, peptides were loaded onto a Thermo NanoViper trap column (75 µm x 20 mm, 3 µm C18, 100 Å) (Thermo Fisher Scientific, Bremen, Germany) using an Agilent 1260 Infinity nanoLC system (Agilent Technologies ...
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bioRxiv - Microbiology 2021Quote: ... using an EASY-nLC 1200 system (peptides were loaded and separated on a 30 cm long home-made C18 column; Thermo Fisher Scientific, Waltham, MA, USA) coupled to a Q Exactive Plus system (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... Four μl of the different peptide mixtures (800 ng for yeast peptides and 949 ng for bulks) were analyzed using an Orbitrap Fusion™ Lumos™ Tribrid™ mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... these peptides were loaded onto the Xbridge BEH300 C18 column (Waters Milford, MA, USA) for HPLC (UltiMate 3000 UHPLC; Thermo Fisher Scientific, Waltham, MA, USA) separation ...
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bioRxiv - Biochemistry 2021Quote: ... For the FAIMS CV scanning of synthetic peptides and glycopeptides, each sample (Table S1, Supporting Information) was directly injected to the Orbitrap Lumos (Thermo Fisher Scientific, San Jose, CA). The CV was scanned from 0-100 V in 1 V steps ...
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bioRxiv - Cancer Biology 2021Quote: ... The eluted peptides were analyzed in a mass spectrometer with an orbitrap mass analyser (Orbitrap Fusion™ mass spectrometer, Thermo Scientific, San Jose, California, USA).
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bioRxiv - Microbiology 2019Quote: ... Samples were resuspended in 20 µL 0.1 % formic acid (FA) and peptides were quantified by measuring the absorbance at 280 nm using a Nanodrop 1000 device (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Immunology 2020Quote: ... Acidified eluted peptides were analyzed in the data-independent acquisition mode on a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) online coupled to an ultra-high-performance liquid chromatography (UHPLC ...
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bioRxiv - Cell Biology 2022Quote: ... One aliquot containing 800 ng of Bradford-quantified peptides was analyzed on an ETD-enabled Orbitrap Velos mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) connected to the EASY-nLC system (Proxeon Biosystem ...
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bioRxiv - Genetics 2022Quote: ... Sodium deoxycholate was removed by acidification with 10 % TFA and the sets were desalted using Pierce peptide desalting spin columns (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions ...
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Multiomic Approach Characterises the Neuroprotective Role of Retromer in Regulating Lysosomal HealthbioRxiv - Neuroscience 2022Quote: ... the resulting peptides were labelled with Tandem Mass Tag (TMT) ten plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK) and the labelled samples pooled.
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bioRxiv - Pharmacology and Toxicology 2022Quote: The eluted peptides were ionized under high voltage (2 kV) and analyzed online using a LumosTM mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA). The initial MS spectrum (MS1 ...
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bioRxiv - Cell Biology 2022Quote: ... 1 µg of the peptide was used for LC-MS/MS analysis which was carried out using a nano-LC 1200 system (Thermo Fisher Scientific, San Jose, CA) coupled to Orbitrap Fusion™ Lumos ETD mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: Peptides were analyzed by LC-MS on a Dionex Ultimate HPLC coupled to an Orbitrap QE Orbitrap mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) operating in Data Dependent Acquisition (DDA ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the modified pSec-NtermHis6 vector containing secretory signal peptide (IgK Leader) as found originally in commercial vector pSecTag/FRT/V5- His-TOPOR vector (Thermo Fisher Scientific, Waltham, MA, USA), but fused to six histidine residues (His6 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were separated on a 25 cm EasySpray™ C18 column (2 μm, 100 Å, 75 μm x 25 cm, Thermo Scientific Cat No: ES902A) at 400 nL/min with a gradient of 4-30% with mobile phase B (Mobile phases A ...
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bioRxiv - Cancer Biology 2023Quote: ... Tryptic peptides from protein digests obtained from cell lines and tissue lysates were quantified by BCA using a peptide digest standard (Thermo Scientific, catalog no. 23225; 23295). Peptides were analyzed by an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... MS spectra and MS/MS spectra were acquired from eluting peptides in a LTQ Orbitrap Velos hybrid mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA), as described previously [77 ...
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bioRxiv - Immunology 2023Quote: ... Protein identification by LC-MS/MS analysis of peptides was performed using an Orbitrap Fusion Lumos Tribid mass spectrometer (Thermo Fisher Scientific, San Jose, CA) interfaced with an Ultimate 3000 Nano-HPLC apparatus (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Mixed peptide samples were prepared from 6 µL of each sample and separated by high pH reversed phase peptide separation kit (Thermo Fisher Scientific, Waltham, MA, USA). Ten fractions were collected by centrifugation ...