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Citations for Thermo Fisher :
3651 - 3700 of 8443 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Chemiluminescent kit was a Supersignal West pico plus chemiluminescent substrate (34577) purchased from Thermo Scientific (Rockford, IL).
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bioRxiv - Synthetic Biology 2021Quote: The optical density was measured at 600 nm using a spectrophotometer (GENESYS 30, Thermo Scientific, IL, USA). The dry cell mass was obtained by multiplication of the optical density of culture broth with a pre-determined conversion factor ...
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bioRxiv - Pathology 2021Quote: ... hMADS adipocytes were differentiated in 2-well Lab-Tek Chamber Slides (Nalge Nunc International, Naperville, IL, USA), washed with PBS pH 7.4 and fixed with 10% paraformaldehyde in PBS for 10 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked and permeabilized in a 10% BSA solution with Triton- X (Thermo Fisher Scientific, Rockford, IL, USA). Subsequently ...
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bioRxiv - Bioengineering 2022Quote: Samples were denatured and reduced using 5× Lane Marker Reducing Sample Buffer (Thermo Scientific, Rockford, IL, US), boiled at 95°C for 10 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Heat denatured protein samples were separated using a 4–12% NuPAGE gel (Thermo Scientific, Rockford, IL, USA). Proteins were transferred to PVDF membranes at 30 volts for 90 minutes ...
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bioRxiv - Microbiology 2022Quote: ... Eight drops of the suspension (2 µl) were placed on Polysine slides (Thermo Scientific/Pierce, Rockford, IL), dried ...
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bioRxiv - Microbiology 2022Quote: ... DNA concentration was measured with the Qubit dsDNA BR Assay Kit (Thermo Fisher Scientific, Rockford, IL, USA); DNA integrity was checked by gel electrophoresis.
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bioRxiv - Physiology 2022Quote: ... and then stained for markers at 5 μg/ml for IL-17A-FITC (Thermofisher, 11-7177-81) and IFNg-PE (Thermofisher ...
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bioRxiv - Neuroscience 2024Quote: ... cells were fixed and immunostained for Iba-1 and cleaved IL- 1β (1:100, PA5-105048, Invitrogen) or CD68 and cleaved caspase 1 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from mouse tissue samples using Trizol LS reagent (Thermo Fisher Scientific, IL, USA). First-strand cDNA synthesis of Isolated RNA was carried out using random primers and high capacity cDNA reverse transcription kit (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... T-cell cultures were supplemented with 200 U/mL IL-2 (Cat # 200-02, Peprotech (Thermo Fisher), Cranbury ...
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bioRxiv - Neuroscience 2023Quote: ... Some of the blots were then stripped (Restore PLUS Western Blot Stripping Buffer; Thermo Scientific, Rockford, IL) and reblotted if needed.
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bioRxiv - Immunology 2023Quote: ... and serum IL-6 and TNF-α levels were measured using a commercial ELISA kit (Thermo Fisher). Serum CD5L levels were measured using ELISA ...
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bioRxiv - Immunology 2023Quote: ... IL-4 and IFN-ᵧ in supernatant was measured according to the manufacturer’s protocol (all Life Technologies).
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bioRxiv - Microbiology 2023Quote: ... the concentration of peptides was measured using Pierce Quantitative fluorometric peptide assay (ThermoFisher Scientific, Rockford, IL, USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... following chemiluminescent detection (SuperSignal® West Pico PLUS and West Femto Chemiluminescent Substrate (Thermo Scientific, Rockford, IL).
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bioRxiv - Cancer Biology 2023Quote: ... ANBL-6 cells were also supplemented with 5 ng/ml IL-6 (Thermo Fisher Scientific, Cat# 206IL010).
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bioRxiv - Cell Biology 2023Quote: ... followed by permeabilization for 3 min with 0.15% Surfact-Amps X-100 (28314, Thermofisher Scientific, Rockford, IL) in PBS ...
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bioRxiv - Physiology 2023Quote: ... membrane was washed and developed using a SuperSignalTM west pico plus chemiluminescent substrate (Thermo Scientific, IL, USA). The blot was developed in iBright 1500 imaging system (Invitrogen ...
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bioRxiv - Genomics 2023Quote: Quantitative measurement of water-soluble protein performed using Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL) [26] ...
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bioRxiv - Immunology 2024Quote: Murine TNF and IL-1β were quantified in cell supernatants using the ELISA™ Kit from Invitrogen and human IL-1β was similarly quantified with the Instant ELISA™ Kit from Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... 1:400 donkey raised rabbit secondary antibody conjugated with Alexa Fluor 488 (Thermo Fisher Scientific, Rockford, IL) and DAPI were incubated for 24 hours at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR quantification of miRNA expression was performed using TaqMan MicroRNA® Assays (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The expression of genes was studied using SYBR green method (Finnzymes F410L, Thermo scientific, Rockford, IL, USA). The Ct values were normalized with actin and the fold differences were calculated using 2-ΔΔCt method.
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bioRxiv - Immunology 2024Quote: ... IL-6 was analyzed from blood serums by sandwich enzyme-linked immunosorbent assay (ELISA; Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... pH 8.5 [28] and were labeled using amine-reactive 16-plex TMT kits (Thermo Scientific, Rockford, IL) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Supernatants were harvested and assayed for IL-8 by ELISA following manufacturer’s instructions (Thermo Fisher Scientific #88808688).
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bioRxiv - Cancer Biology 2021Quote: ... Microarray was performed by the UVA DNA Sciences core using the Human Affymetrix GeneChip Array ([HuGene-2_1-st] Affymetrix Human Gene 2.1 ST Array). Gene lists were generated from microarray data corresponding to genes >1.25 LogFC and <0.75 logFC were used in downstream analyses ...
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bioRxiv - Microbiology 2022Quote: Cytokines in the cell culture supernatants were measured using the human cytokine storm 21-plex procartaplex panel human magnetic bead Luminex assay (Thermo Fisher, EPX210-15850-901), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: Concentrations of FGF19 in human serum samples were tested using a commercial Human Fibroblast Growth Factor 19 Assay Kit (Thermo scientific, Waltham, MA, USA). The level of serum C4 was quantified by a mass spectrometry-based method developed by our group.32
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bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Two control samples from the same pool of total human brain RNA (Ambion FirstChoice human brain reference RNA Cat#AM6050, Life Technologies Inc., Carlsbad, CA) were included with each of the five datasets ...
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bioRxiv - Synthetic Biology 2021Quote: ... supplemented with anti-human CD3/CD28 magnetic Dynabeads (CTS, ThermoFisher) in a 1:1 ratio with cells ...
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bioRxiv - Cell Biology 2020Quote: Human fibroblast cells lines were maintained in DMEM/F12 (Gibco) with 15% FBS (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human fibroblasts were grown in MEM (Gibco, Life Technologies) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... Primary human fibroblasts were grown in MEM (Gibco, Life Technologies) supplemented with 20% fetal calf serum (FCS ...
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bioRxiv - Cell Biology 2020Quote: ... 20 ng/ml recombinant human epidermal growth factor (Invitrogen, USA), 10 ng/ml human basic FGF (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... PE-conjugated mouse anti-human CD270 (ThermoFisher Scientific, MA, USA), and Pacific Blue-conjugated mouse anti-human CD274 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... FITC-conjugated mouse anti-human SOX2 (ThermoFisher Scientific, MA, USA), BV421-conjugated mouse anti-human CD34 (Biolegend ...
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bioRxiv - Developmental Biology 2021Quote: Human PSCs resistant to neomycin were incubated with Accutase (Gibco) in 37°C for 5-10 minutes ...
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bioRxiv - Immunology 2021Quote: ... Human staining panels included anti-CD3-Alexa 700 (Invitrogen, UCHT1), anti-CD19-APC (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... human recombinant Epidermal Growth Factor (5 ng/ml, ThermoFisher Scientific) and 0.15 mM CaCl₂ (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... anti-human IgG secondary antibody labeled with Dylight 650 (Invitrogen) was applied at 1 to 1000 (∼0.5μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... and 20 ng/mL human heat stable sbFGF (Gibco, PHG0367) and split using accutase (Millipore Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... coated with 5μg/ml human recombinant vitronectin (Life Technologies, #A14700) in mTesR1 (StemCell Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... 0.36 pM Recombinant Human Bone Morphogenetic Protein 4 (BMP4, Gibco), and 0.08 pM Activin A (Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... and human recombinant epidermal growth factor (EGF; Gibco 10450-013). All cells were cultured at 37 °C and 5% CO2.
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bioRxiv - Immunology 2022Quote: ... supplemented with 2% AB human serum (Gibco; Thermo Fisher Scientific). T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).
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bioRxiv - Immunology 2022Quote: ... supplemented with 2% AB human serum (Gibco; Thermo Fisher Scientific). T cell lines were cultured for 10 days in the presence of 20 U/ml of recombinant IL-2 (R&D Systems).