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Citations for Thermo Fisher :
3451 - 3500 of 8443 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... human Midkine (100 ng/ml, ThermoFisher Scientific, 450-16), human Bmp7 (100 ng/ml ...
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bioRxiv - Cell Biology 2024Quote: ... primary human islets were first dissociated with TrypLE (Gibco) prior to incubation with a mouse monoclonal anti-human NTPDase3 antibody (Ectopeptidases ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... (Telangana, India) with human suspension hepatocytes (HMCS1S, Gibco USA). 200 µL of cell suspension containing 2 x 106 hepatocytes/mL (>95% viability ...
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bioRxiv - Systems Biology 2024Quote: ... ANXA1 (rabbit anti-human, 1:1000, ThermoFisher Scientific, UK), phosphotyrosine-21 ANXA1 (rabbit anti-human ...
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bioRxiv - Cell Biology 2024Quote: ... or two siRNAs targeting human PLA2G15 (ThermoFisher s24296, s24297) using RNAiMax (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... human astrocytes at P4 (K1884, Thermo Fisher Scientific Gibco) were seeded in each well ...
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bioRxiv - Neuroscience 2024Quote: ... human astrocytes at P4 (K1884, Thermo Fisher Scientific Gibco) were seeded in each well ...
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bioRxiv - Neuroscience 2024Quote: ... for human HTT and Alexa fluor 647 (Thermofisher # B40958) for mouse Htt ...
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bioRxiv - Neuroscience 2024Quote: ... AT8 human PHF-Tau (Thermo Scientific, MN1020, 1:250). The following secondary antibodies were used ...
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bioRxiv - Bioengineering 2024Quote: Human dermal fibroblast (HDF) cells (C0135C; ThermoFisher Scientific, UK) were cultured in flasks with Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Cancer Biology 2024Quote: ... rabbit anti-human CD3 (clone SP7, Thermo Fisher Scientific); sheep anti-human CD34 (#AF7227-SP ...
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bioRxiv - Developmental Biology 2024Quote: ... in 2 mL complete human medium (DMEM/F12 [Gibco, Massachusetts ...
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bioRxiv - Immunology 2024Quote: ... Dynabeads™ Human T-Activator CD3/CD28 (ThermoFisher Scientific) were added to the culture ...
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bioRxiv - Microbiology 2024Quote: ... Goat anti-human IgG (H + L) (Thermo Fisher Scientific) or Goat anti-Human IgG Fc recombinant (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Goat anti-human IgG (H+L) (Thermo Fisher Scientific) pre-coupled to Alexa Fluor 647 were used as secondary antibodies in flow cytometry experiments ...
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bioRxiv - Molecular Biology 2024Quote: ... Human PA-1 cells were cultured in MEM (Gibco) supplemented with GlutaMAX ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the human σ2 receptor was cloned into pcDNA3.1 (Invitrogen) mammalian expression vector with an amino-terminal protein C tag followed with a 3C protease cleavage site and transfected into Expi293 cells (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... or Goat anti-Human IgG Fc recombinant (ThermoFisher Scientific) antibodies were pre-coupled to Alexa Fluor 647 and used as secondary antibody in flow cytometry experiments ...
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bioRxiv - Cell Biology 2020Quote: The frozen supernatants were used for IL-2 ELISAs according to the manufacturer’s protocol (KMC0021, Thermofisher).
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bioRxiv - Immunology 2021Quote: ... containing the cytokines IL-4 (500 U/mL) and GM-CSF (800 U/mL) (both Gibco) for differentiation into DCs ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein concentration was evaluated using the Modified Lowry Protein Assay (Thermo Fisher Scientific, Rockford, IL, USA). Samples were prepared for SDS-PAGE in Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Pathology 2022Quote: Total RNA was extracted from the heart using TRIzol reagent (Thermo Fisher Scientific, Rockford, IL, USA) as reported previously.8 Total RNA was reverse transcribed into cDNA using random primers (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was determined using the Qubit® Protein Assay Kit (Thermo Scientific Rockford, IL, USA). For both cases ...
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bioRxiv - Neuroscience 2021Quote: ... The protein band was detected using Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL).
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bioRxiv - Molecular Biology 2021Quote: The LysoTracker Red assay was used to follow the manufacturer’s protocol (LifeTechnology, ThermoFisher, Rockford, IL, USA). LysoTracker Red (50 nM ...
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bioRxiv - Molecular Biology 2021Quote: ... 5% sodium dodecyl sulfate (SDS) in 50 mM triethylammonium bicarbonate (TEAB, Thermo Fisher Scientific, Rockford, IL), pH=8.0) ...
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bioRxiv - Cell Biology 2022Quote: ... Cell lysates were prepared in the modified RIPA buffer containing protease inhibitors (Thermo Scientific, Rockford, IL). Equal amounts of proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Protein concentration was estimated by BCA (Pierce BCA Protein Assay Kit, Thermo Scientific, Rockford, IL, USA). Denatured proteins were reduced with 5 mM DTT ...
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bioRxiv - Biochemistry 2019Quote: Primary enriched CM and hiPSC-CM were lysed in 1X Invitrosol (Thermo Fisher Scientific, IL, USA), 100 mM ammonium bicarbonate (NH4HCO3) ...
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bioRxiv - Immunology 2019Quote: ... The protein concentrations were determined by the bicinchoninic acid assay (BCA, Pierce Biotechnology, Thermo Scientific, IL). Further processing was adapted from a filter-aided sample preparation (FASP ...
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bioRxiv - Physiology 2020Quote: ... The PCR amplifications were performed with SYBR green qPCR master mix (#K0252, Thermo Scientific, Rockford, IL). Total RNA was prepared from the frozen whole uterine and placental tissues ...
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bioRxiv - Biophysics 2019Quote: ... proteins were purified using HisPur Cobalt Resin according to the manufacturer’s protocol (Thermo Scientific, Rockford, IL.) and Ulp-1 protease (Ubl-specific protease 1 ...
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bioRxiv - Neuroscience 2019Quote: ... Protein concentration was measured using Pierce BCA protein assay kit (Thermo Fisher scientific Inc., Rockford, IL). CHT-mediated choline uptake was determined as total choline uptake minus the uptake in the presence of hemicholinium-3 and maximum transporter velocity (Vmax ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected and used for cytokine measurement with IL-18 (Invitrogen; sensitivity 19.0 pg/mL), IL-1β (Invitrogen ...
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bioRxiv - Bioengineering 2021Quote: Pierce Bicinchoninic acid (BCA) and MicroBCA protein assay kits were purchased from Thermo Scientific (Rockford, IL). Quant-iT PicoGreen dsDNA assay kit was procured from Molecular Probes ...
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bioRxiv - Biochemistry 2021Quote: ... Gels were transferred onto Biodyne B nylon membranes (0.45 μm; Thermo Fisher Scientific, Rockford, IL, USA) in 0.5X TBE buffer (60V for 30 min) ...
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bioRxiv - Biochemistry 2021Quote: ... that contained 1mg/ml Texas Red-dextran (10,000 MW, Lysine Fixable, Thermo Fisher Scientific, Rockford, IL) at 37°C for 6-24 hr ...
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bioRxiv - Cell Biology 2021Quote: ... M1 tri-Ub was purified to homogeneity by Ni2+-NTA affinity column (Thermo Scientific, Rockford, IL) chromatophraphy ...
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bioRxiv - Cell Biology 2021Quote: ... Protein content was determined using the bicinchoninic acid assay (BCA) (Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Biochemistry 2020Quote: ... The following antibodies were used: mouse monoclonal anti-V5 (Thermo Fisher, Rockford, IL, USA, 1:1,000) and rabbit polyclonal anti-FLAG (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Protein concentration was determined by the Bicinchoninic acid method (Pierce, ThermoFisher Scientific Fisher, Rockford, IL, USA). Aliquots of lysates containing 30 μg of protein were separated on SDS-PAGE and transferred to Immobilon-P membranes ...
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bioRxiv - Cell Biology 2021Quote: Samples were analyzed on Orbitrap Fusion Lumos Plus mass spectrometers (Thermo Fisher Scientific, Rockford, IL, USA) coupled with an Easy-nLC 1000 nanoflow LC system (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were stained with LIVE/DEADTM fixable Aqua dead cell stain (Thermo Fisher Scientific, Rockford, IL) for 30 min at 4oC ...
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bioRxiv - Biochemistry 2022Quote: ... Using Slide –A-Lyzer –Minidialysis units with a 10,000 Dalton MWCO(Thermo Scientific, Rockford, IL, USA) proteins were renatured by dialyzing out urea in a step wise manner from 6 M in steps of two at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... in a 1:1 ratio and maintained in R10 + IL-2 [RPMI 1640 (Thermo Fisher Scientific) supplemented with 10% heat-inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... PKCα A (Cat# s11092) or PKCα B (Cat# s11094) silencer®select siRNA (Thermo Scientific, IL) using the lipofectamine RNAiMAX reagent as we described previously (24).
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bioRxiv - Neuroscience 2020Quote: ... RNA quality and concentration were measured using a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific, Chicago, IL). 500-1000 ng RNA per sample was reverse-transcribed using Applied Biosystems high-capacity reverse transcription kit (Applied Biosystems ...
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bioRxiv - Physiology 2021Quote: ... were purchased from Badrilla (Leeds, UK), while RyR2 (1:1000, #MA3-916) was purchased from Thermo Fisher (Rockland, IL, USA). The primary antibodies against p62 (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... then protein was quantified using BCA protein assay kit (Thermo Scientific, Rockford IL, USA, Ref. 23227) using bovine serum albumin (BSA ...
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bioRxiv - Immunology 2019Quote: ... the peptides were labelled with biotin by using Sulfo-NHS-LC-Biotin (Thermo Scientific, IL, USA). The unreacted Sulfo-NHS-LC-Biotin was quenched by 1 M glycine solution ...