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Citations for Thermo Fisher :
3501 - 3550 of 8443 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Mouse TNF-α and IL-6 ELISA Ready-SET-Go! kits (Affymetrix, San Diego, CA, USA) were used for the detection of tumor necrosis factor α (TNF-α ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with Alexa Fluor 633-conjugated Phalloidin at 0.005 units/μl (Molecular Probes/ Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with Alexa Fluor 633-conjugated Phalloidin at 0.005 units/μl (Molecular Probes/ Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... each protein pellet was digested with 2 µg sequencing grade trypsin (Thermo Scientific, Rockford, IL, USA) overnight at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... cells were seeded into Lab-Tek CC2 treated slide chambers (Nalgene Nunc International, Napeville, IL, USA); after washing with PBS cells were fixed with 4% paraformaldehyde/PBS for 10 min ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were harvested and assayed for IL-8 by ELISA (Thermo Fisher Scientific #88-8086-77). Alternatively ...
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bioRxiv - Microbiology 2020Quote: ... and soluble Mpro proteins were purified with an Ni-NTA resin (Thermo-Fisher Scientific, Rockford, IL) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... and cloned using CloneJET PCR Cloning Kit (K1231, Thermo Scientific, Fisher Scientific, Hanover Park, IL, USA). Full-length duck ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with Alexa Fluor 488-conjugated Phalloidin at 0.005 units/αl (Molecular Probes/Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with Alexa Fluor 488-conjugated Phalloidin at 0.005 units/αl (Molecular Probes/Invitrogen, Rockford, IL) at room temperature for 1 h ...
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bioRxiv - Immunology 2021Quote: ... TaqMan gene expression kits were utilized to measure target genes of interest: Il-1β (Mm00434228_m1, ThermoFisher), Il-6 (Mm00446190_m1 ...
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bioRxiv - Bioengineering 2022Quote: ... sera from individual mice were added to peanut coated Maxisorp Immunoplates (Nalge Nunc International, Naperville, IL). Peanut-specific IgG1 was detected using goat anti-mouse IgG1-HRP conjugated (Southern Biotechnology Associates ...
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bioRxiv - Bioengineering 2022Quote: ... sera from individual mice were added to peanut coated Maxisorp Immunoplates (Nalge Nunc International, Naperville, IL). Peanut-specific IgE Abs were detected with goat anti-mouse IgE-unlabeled (Southern Biotechnology Associates ...
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bioRxiv - Synthetic Biology 2022Quote: ... the yeast genomic DNA was extracted with Yeast DNA Extraction Kit (Thermo Fisher, Rockford, IL, USA). Three primers ...
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bioRxiv - Microbiology 2022Quote: ... before it was developed with SuperSignal West Dura Extended Duration substrate (Thermo Fisher Scientific, IL, USA). The blot was imaged using a Bio-Rad Chemidoc XRS+ (Bio-Rad ...
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bioRxiv - Pathology 2022Quote: ... Goat anti Rabbit Alexa Fluor 647 and Goat anti-Mouse Alexa Fluor 568 (Invitrogen, Rockford IL) were used ...
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bioRxiv - Systems Biology 2022Quote: ... The cell lysates were prepared in RIPA buffer containing protease inhibitors (Thermo Fisher Scientific, Rockford, IL) immediately after treatment ...
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bioRxiv - Neuroscience 2022Quote: ... NPC cultures received 100 pM acetic acid vehicle or 100 ng/ml IL-6 (Gibco; PHC0066) on day 18 for 3h ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were used to perform ELISA to quantify the levels of IL-1β (Thermo Fisher Scientific), IL-6 (Biolegend) ...
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bioRxiv - Genetics 2022Quote: ... cDNA was amplified using High-Capacity RNA-to-cDNA™ Kit (Applied Biosystems, Rockford, IL, USA). The GCLC sequence was amplified using the following primers ...
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bioRxiv - Microbiology 2024Quote: ... Formulations were dialyzed against PBS (pH 7.2) in the dialysis cassettes (Thermo Scientific, Rockford, IL, USA) for at least 18 h ...
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bioRxiv - Cell Biology 2024Quote: ... and activated 1:1 with CD3/CD28 Dynabeads in 100 U/mL of IL-2 (Thermofisher). The next day ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using SYBR select master mix (Thermo Fisher Scientific, IL, USA). Amplification was accomplished over 30 cycles as follows ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using SYBR select master mix (Thermo Fisher Scientific, IL, USA). The reaction conditions were as follows ...
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bioRxiv - Immunology 2024Quote: ... Real-time quantitative PCR was performed using SYBR select master mix (Thermo Fisher Scientific, IL, USA). The reaction conditions were as follows ...
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bioRxiv - Immunology 2024Quote: ... The cells were treated for 18-24 hours with 10 ng/mL IL-1β (Gibco, #PHC0815) and 5 ng/mL TNF (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were harvested for IL-1β measurement using a specific ELISA (Thermo Fisher, 88-7621-22). Apoptotic cells were generated by retroviral transduction or MTX treatment during 20-24 h when cell death approaches its maximum ...
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bioRxiv - Biophysics 2023Quote: ... and then immunoreactive bands were visualized using Super Signal WestPico chemiluminescent substrate (Thermo Scientific, Rockford, IL). Densitometric analysis of nonsaturated films was performed using Bio-Rad Quantity One analysis software (Hercules ...
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bioRxiv - Neuroscience 2023Quote: ... Serum was run through an interleukin-6 (IL-6) enzyme-linked immunoabsorbent assay (ELISA) kit (Invitrogen). Successful maternal immune activation in offspring of Poly(I:C ...
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bioRxiv - Molecular Biology 2023Quote: ... or inserts in 24-well plates (0.4 μm polyester membrane, CoStar, Thermo Fisher Scientific, Rockford, IL) until 90% confluence was achieved(102) ...
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bioRxiv - Biochemistry 2023Quote: ... Nuclear fractions were obtained using NE-PER Nuclear and Cytoplasmic Extraction Reagents (Thermo Scientific, IL, USA).
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bioRxiv - Neuroscience 2023Quote: ... Detection was performed through the chemiluminescence assay with an ECL plus kit (Thermo Scientific, IL, USA). Densitometric detection was carried out with the LI-COR Odyssey imager and Image Studio software (version 5.2 ...
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bioRxiv - Immunology 2023Quote: ... LUVA supernatants were assayed for IL-6 and TNF (Invitrogen, #88-7066-88, #88-7346-88). β-hexosaminidase was measured in fresh supernatants by transferring 20µl sample/well to a 96 well plate (Sarstedt ...
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bioRxiv - Cell Biology 2023Quote: ... and the resultant tryptic peptides were labeled with acetonitrile-dissolved TMT reagents (Thermo Scientific, Rockford, IL), and equal amounts of labeled samples were mixed before prefractionation with reversed phase (RP)-high performance liquid chromatography (HPLC) ...
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bioRxiv - Immunology 2023Quote: ... The gels were fixed and stained with Blue Safe Protein Stain (Thermo Scientific, Rockford, IL, USA) or transferred to a PVDF membrane using a Trans-Blot Turbo system (Bio-Rad ...
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bioRxiv - Genomics 2023Quote: ... The blots were then developed using the ECL western blotting substrate (Thermo Scientific, Rockford, IL, USA). The levels of protein expression were semi-quantified by optical densitometry using Image J Software version 1.46 (49) ...
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bioRxiv - Pathology 2023Quote: ... It was determined using a Pierce® BCA protein assay kit (Thermo Scientific, Rockford, IL, USA) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and IL-12 was quantified with ELISA kits according to the manufacturer’s instructions (Invitrogen, CA, USA).
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bioRxiv - Microbiology 2023Quote: ... supplemented with 1x protease inhibitor (Pierce Protease Inhibitor Tablet, EDTA-Free, ThermoFisher Scientific, Rockford, IL, USA) and the light units (LU ...
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bioRxiv - Immunology 2024Quote: ... Th1 consisted of 1 µg/mL anti-murine IL-4 (Cat# 16-7041 Invitrogen Carlsbad, CA), 5 ng/mL murine IL-2 (Cat# 212-12 Peprotech Cranbury ...
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bioRxiv - Biochemistry 2024Quote: Fisher OptimaTM grade reagents were used for sample preparation and analysis (Fisher Scientific, Hanover Park, IL). EquiSPLASH® LIPIDOMIX (Avanti Polar Lipids Inc. ...
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bioRxiv - Immunology 2024Quote: ... IL-5 levels were quantified in undiluted BAL fluid by ELISA following the manufacturer’s instructions (Invitrogen).
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bioRxiv - Genomics 2021Quote: ... Lysates and positive technical controls (Human Universal Reference RNA - uhrRNA Agilent Cat# 740000, Santa Clara, California and Human Brain Total RNA brRNA - ThermoFisher AM7962, Waltham, Massachusetts), as well as no-cell negative controls (1X TempO-Seq lysis buffer alone ...
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bioRxiv - Immunology 2020Quote: The assay to measure total IgG was modified to measure IgG subclass responses at 1/100 including a two-step biotinylated anti-human IgG subclass (mouse anti-human IgG1: HP6069, IgG2: HP6002, IgG3: HP6050 IgG4: HP6023, Thermo fisher Scientific, UK) with a streptavidin-Phycoerythrin tertiary (Thermo fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were run on the Clariom™ S Human Array to measure gene expression at >20,000 genes in the human genome (Affymetrix, Santa Clara, CA). Raw data generated from the arrays were read into R statistical software (version 3.5.1 ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor™ 488-Human Transferrin conjugate (Cat# T13342) and DiI–human LDL conjugate (Cat# L3482) were obtained from Molecular Probes (Eugene, OR). Primer kits and Master Mix for RT-qPCR were purchased from Applied Biosystems (Foster City ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Microbiology 2021Quote: ... Viral RNA levels were normalized to non-human primate β-actin (Table S1) or human β-actin (Hs99999903_m1, Applied Biosystems; Thermo Fisher Scientific) and calculated by the ΔΔCt method [46].
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bioRxiv - Immunology 2021Quote: PBMC derived CD4+ T cells were isolated from HIV-infected MACS participants using EasySep™Human CD4+ T Cell Isolation Kit and activated overnight with Human T-Activator CD3/CD28 Dynabeads® (Life Technologies). The next day Dynabeads were separated from the CD4+ T cells by manual dissociation followed by magnet isolation ...
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bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...