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Citations for Thermo Fisher :
3601 - 3650 of 8443 citations for IL 12RB1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Expression of the recombinant LZTR1 proteins was confirmed by Western blot analysis using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific). Following confirmation of expression ...
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bioRxiv - Immunology 2022Quote: ... Expi293F™ cells were transiently transfected with His-tagged gB ectodomain plasmids for five days and later harvested with Nickel-NTA resin (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2023Quote: The baculovirus containing either His-mCherry-HT1 or twin strep-mVenus-MPK12 was constructed using the Bac-to-Bac™ system (Thermo Scientific). Resulting baculoviruses were used to co-infect 600 ml of Sf9 insect cells in the shaker culture at the density of 2×106 cells/ml at approximate multiplicity of infection 5-10 ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were washed once with ice-cold PBSB and incubated with 1000x dilution of mouse anti-Myc and chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Microbiology 2023Quote: The Lysobacter enzymogenes gasdermin and the Pedobacter rhizosphaerae system were both expressed under an arabinose-inducible PBAD promoter in the pBad/His A plasmid (Thermo Scientific V43001). The Lysobacter gasdermin system was previously cloned10 and the Pedobacter rhizosphaerae system was amplified from genomic DNA ordered from the DSMZ (DSM 18610 ...
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bioRxiv - Biochemistry 2023Quote: ... of randomized RNA library produced for IVM-seq experiment was incubated with equal molarity of 6x His-tag fused target protein complex immobilized on 7 μL Ni-NTA magnetic agarose beads (Thermo Scientific 78605) in the SELEX binding buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... The lysate was cleared by centrifugation at 20,000 x g for 30 min at 4 °C and purified by using GE Healthcare His GraviTrap™ Kit (Cat. No. 45-002-016, Fisher Scientific). After elution ...
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bioRxiv - Bioengineering 2023Quote: ... Yeast cells were incubated with 1:1000 dilution of mouse anti-Myc antibody and 1:1000 dilution of chicken anti-His (Invitrogen, PA1-9531) antibodies on ice for 20 min ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies against ZNF524 were purified and enriched from the serum against this recombinantly expressed His-ZNF524 using the SulfoLink® Immobilization Kit for Peptides (Thermo Scientific) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... were expanded and maintained in MEMα (GibcoTM 22561021) supplemented with 10% heat-inactivated FBS (HI-FBS; LabTech FB-1001/500 or Gibco 10438-026) at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: pMT-Reps-Flag and pMT-Reps-HA were generated by cloning Reps-RA isoform from FMO04419 (DGRC Stock 1612020) into pMT-V5-His vector (Invitrogen cat#V412020). pMT-Mnb-V5 was generated by cloning Mnb-RH isoform from FMO12028 (DGRC Stock 1639183 ...
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bioRxiv - Biophysics 2024Quote: ... Lysates containing Utrophin or α-actinin were loaded on a column packed with His-Pur Ni-NTA resin (ThermoFisher Scientific, Waltham, MA). The column was washed with 0.6 M NaCl ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Molecular Biology 2024Quote: The piggyBac piRNA target site was inserted into the middle of the V5 coding sequence in the pIB-V5/His vector (Thermo Fisher Scientific) by site-directed mutagenesis ...
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bioRxiv - Neuroscience 2022Quote: ... Live brain slices were incubated in modified ACSF containing 1μm SR101 (Thermo Fisher Scientific, Rockford, IL, USA) at 34 °C for 20min ...
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bioRxiv - Neuroscience 2021Quote: ... Levels of TNFα and IL-6 in culture supernatants were measured using mouse ELISA kits (Thermo Scientific).
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bioRxiv - Bioengineering 2022Quote: ... wells were then incubated for 1 hour at room temperature with biotinylated antibody against IL-10 (Invitrogen) and then incubated with HRP-conjugated streptavidin (Invitrogen ...
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bioRxiv - Immunology 2022Quote: Cell culture supernatants from BMDCs were analyzed by ELISA for IL-12p70 (R&D) and IFNβ (Invitrogen). Plates were read using a Tecan Sunrise microplate reader at 450 nm ...
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bioRxiv - Bioengineering 2019Quote: ... crude and IL-treated anammox granules were stained with BacLight Live/Dead viability stain (Thermo Fisher Scientific) according to manufacture’s manual ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... RNA quantity and quality were evaluated by a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Rockford, IL, USA). Then ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentrations were determined using the Bicinchoninic Acid Protein Assay kit (Pierce/Thermo Scientific, Rockford, IL, USA).
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bioRxiv - Immunology 2019Quote: ... and then grown in the absence of IL-2 for 24 h in RPMI-1640 (Gibco, # 21875034) medium supplemented with 10% FBS (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration of lysates was determined with the Pierce 660 Protein Assay Reagent (Thermo Scientific, Rockford, IL).
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bioRxiv - Neuroscience 2020Quote: ... The tissue was homogenized and sonicated in T-Per lysis buffer (Thermo Scientific, Rockford, IL, United States). The protein concentration of the samples was determined using a Pierce BCA Protein Assay Kit (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Protein levels of IL-18 from intestinal biopsies were measured by ELISA (BMS618-3, ThermoFisher, Waltham, MA). Assays were performed according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... Cell nuclei were counterstained with 0.5 μg/ml DAPI for 5 min (Thermo Fisher Scientific, Rockford, IL). The following primary antibodies were used ...
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bioRxiv - Physiology 2021Quote: ... IL-6 (assay ID: HS00985639_m1) and TNF-α: (assay ID: HS00174128_m1) following manufacture’s protocol (Thermo Fisher Scientific). 18s (assay ID ...
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bioRxiv - Cancer Biology 2020Quote: ... RNA was quantified using the Qubit™ RNA High Sensitivity Assay Kit (Thermo Fisher Scientific, Rockford, IL). Sample integrity was evaluated using the Agilent Bioanalyzer RNA Nano and Pico kits (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibody (diluted 1:10,000) and SuperSignal® West Femto Maximum Sensitivity Substrate (Thermo Scientific, Rockford, IL).
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bioRxiv - Biophysics 2020Quote: ... or Zeba micro spin™ desalting colums 0.5 ml (7000 MWCO, Thermo Fisher Scientific, Rockford, IL, USA) were used ...
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bioRxiv - Immunology 2022Quote: ... TNFα and IL-10 levels were measured by TNFα Mouse Uncoated ELISA Kit (88-7324-86, Invitrogen) and IL-10 Mouse Uncoated ELISA Kit (88-7105-86 ...
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bioRxiv - Immunology 2022Quote: ... Protein was visualized using the SuperSignal West Pico ECL kit (Pierce – Thermo Fisher Scientific, Rockford, IL, USA).
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bioRxiv - Molecular Biology 2020Quote: ... RNA was reverse transcribed with the SuperScript IV First-Strand Synthesis System (Thermo Fisher Scientific, Rockford, IL). cDNA was PCR amplified using the mEX11F and mEX13R primers and subjected to Sanger sequencing.
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bioRxiv - Biochemistry 2021Quote: ... 0.1% SDS) supplemented with 1X Halt protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific, Rockfold, IL, USA) and 1mM phenylmethylsulfonyl fluoride (PMSF ...
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bioRxiv - Molecular Biology 2020Quote: Chromatographic separation was achieved on an Accucore C30 column (2.6µm, 3mm x 150mm, Thermo Scientific, Rockford, IL) with linear gradient elution consisting of mobile phases A (water/acetonitrile = 40:60 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration was measured using a BCA reducing agent compatible assay kit (Thermo Scientific, Rockford, IL, USA). Proteins were precipitated by adding ice-cold acetone overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... Calibration was performed before each analysis using a positive calibration mix (Piercenet – Thermo Fisher, Rockford, IL, USA). Limits of detection (LOD ...
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bioRxiv - Developmental Biology 2022Quote: ... The ATP concentration of the supernatant was calculated by the ATP Determination Kit (Thermo Scientific, Rockford, IL) relative to a standard ...
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bioRxiv - Synthetic Biology 2019Quote: The optical density was measured at 600 nm using a spectrophotometer (GENESYS 30, Thermo Scientific, IL, USA). The dry cell mass was obtained by multiplication of the optical density of culture broth with a pre-determined conversion factor ...
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bioRxiv - Microbiology 2019Quote: Amounts of IFNγ and IL-6 in BALF were estimated by ELISA Ready-SET-Go! Kit (Invitrogen) according to manufacturer’s protocol.
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bioRxiv - Cancer Biology 2019Quote: ... cells were homogenized and resuspended in radioimmunoprecipitation assay (RIPA) lysis buffer (Thermo Fisher Scientific, Rockford, IL, USA) containing a cocktail of protease inhibitors (Roche ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Values were corrected for protein concentration as determined with a BCA assay kit (Thermo Scientific, Rockford, IL). All values were determined in triplicate ...
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bioRxiv - Physiology 2019Quote: ... Gels were washed and proteins were stained with Imperial Protein Stain (Thermo Fisher Scientific, Rockford, IL, USA) for 2 hours ...
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bioRxiv - Immunology 2019Quote: ... IL-2 mRNA amounts were determined with a SYBR Green PCR master mix from Life Technologies (4344463) relative to mRNA for β-2 microglobulin on a DNA Engine Opticon II System (Bio-Rad ...
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bioRxiv - Physiology 2019Quote: ... The quantity of the RNA was determined on a Nanodrop ND-1000 (Thermo Scientific, Rockford, IL, USA). Reverse-transcriptase PCR was performed using the Multiscribe Reverse Transcriptase method (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... and eluted with imidazole (gradient 10–200 mM) and dialyzed (prod. no. 68100, Thermo Scientific, Rockford, IL). In the case of Sec14 ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration of cells was measured using a Micro BCA Protein Assay Kit (Thermo Scientific, Rockford, IL) and used to normalize cytokine levels with final concentrations adjusted to an equivalent of 25 μg of protein for comparison across experiments.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Total protein content in the homogenate was estimated using the BCA assay (Thermo Fisher Scientific, Rockford, IL), according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2019Quote: ... The blots were then developed using Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, Il, USA) and signals were captured by using high sensitive CCD camera-based imager (BioSpectrum Imaging System) ...
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bioRxiv - Physiology 2021Quote: ... The total protein was inoculated with anti-HA magnetic beads at 4 C (Thermo Scientific, Rockford, IL) overnight ...