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Citations for Thermo Fisher :
3501 - 3550 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Expression of toxins was induced by the addition of isopropyl β-D-thiogalactopyranoside (IPTG, Fisher Scientific) to a final concentration of 1 mM when the OD600 of the culture reached 0.5-0.6 after which the cultures were allowed to grow overnight ...
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bioRxiv - Plant Biology 2024Quote: ... Resuspended cells were then seeded onto a 96-well plate coated with Poly-D-lysine (Gibco) and containing fresh culture media ...
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bioRxiv - Evolutionary Biology 2024Quote: We subsequently rinsed with PBS-d (0.1M PBS [BR0014G, ThermoFisher Scientific, MA, USA] with 1% DMSO), performed a 30 minute permeabilization wash with permeabilization buffer (PBS-d and 2% Triton-X-100) ...
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bioRxiv - Microbiology 2024Quote: ... (ATCC HTB-55) were cultured DMEM with 1.0 g/L D-glucose (Gibco – cat #12320-032) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2024Quote: ... Vero cells were maintained in DMEM containing D-glucose (4.5 g/l) and L-glutamine (Gibco), supplemented with 2% heat-inactivated and filter-sterilized fetal bovine serum (Gemcell).
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized with d(T)23VN primer using SuperScript II Reverse Transcriptase (Thermo Fisher Scientific). α1 and β1 coding sequences corresponding respectively to α1-PA and β1-PA were amplified with primers containing EcoRI and XhoI sites and cloned into EcoRI-XhoI digested pUASTattB.114 pUASTattB-α1 and pUASTattB-β1 were integrated into attP40 (BestGene Inc).
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bioRxiv - Molecular Biology 2024Quote: ... Fresh medium containing the selected DSB agent and 50 μM D-Biotin (Thermo Fisher Scientific, B20656) were added and incubated for 1h ...
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bioRxiv - Bioengineering 2024Quote: ... Drug selection began 2 d post transduction with 1 μg/mL puromycin (Invitrogen, ant-pr-1). Cells were kept in antibiotics for at least two weeks with subculturing every one to two days before further characterization.
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bioRxiv - Molecular Biology 2024Quote: ... sequencing of cDNA libraries was performed using Affymetrix Clariom D (Thermo Fisher Scientific, Saint Louis, MO) array at the Genomics Core Facility of the University of Kansas Medical Center as described before37,69 ...
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bioRxiv - Plant Biology 2024Quote: ... and the 1.4kb PCR product was transferred into the pENT-D-Topo plasmid (Thermofisher, Rochester, USA). This entry vector was used to construct the pET300-PII1 vector by using the gateway technology into the ChampionTM pET300 plasmid (Thermofisher ...
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bioRxiv - Plant Biology 2024Quote: ... The amplified fragment was cloned into the pENTR/D-TOPO cloning vector (Life Technologies, Rockville, USA) to generate the pENTR-MpRLFpro vector ...
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bioRxiv - Neuroscience 2024Quote: ... coated with coverslips (Neuvitro, GG-12) pre-treated with poly-D-lysine (Thermo Fisher Scientific, A3890401), for staining and calcium imaging ...
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bioRxiv - Immunology 2024Quote: ... Mice were imaged 5 minutes after intraperitoneal injection with D-luciferin (150 mg/kg) (Thermo Scientific). Luciferase signal was quantified using Living Image software (Caliper LifeSciences) ...
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bioRxiv - Biophysics 2024Quote: ... expression was induced with 0.5 mM IPTG (isopropyl β-D-1-thiogalacto-pyranoside, Thermo Fisher Scientific) at OD600 = 0.6-0.8 and subsequently cultured at 16 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were plated on poly-d-lysine coated glass coverslips (cat. A3890401, ThermoFisher, Waltham, MA, USA) at 100,000 cells/well and matured until day 30 ...
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bioRxiv - Cell Biology 2024Quote: ... Following metabolic labeling for 2 hr cultured neurons were washed two times with D-PBS (Gibco) on ice and harvested in D-PBS by scraping and a 40-second spin-down in a small bench top mini centrifuge ...
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bioRxiv - Cell Biology 2024Quote: ... Organoid lysates were prepared in 100 μL of D-PBS with protease inhibitors (A32955, Thermo Fisher) and sonicated on ice for 30 seconds at an amplitude of 10-14 ...
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bioRxiv - Cancer Biology 2024Quote: Human fibrosarcoma HT1080 were grown in complete Dulbecco’s modified Eagle’s medium (D-MEM, Thermo Fisher Scientific) supplemented with 10% heat inactivated fetal calf serum penicillin/streptomycin (100 U/ml ...
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bioRxiv - Bioengineering 2024Quote: ... and 14 d after seeding to assess cell attachment using LIVE-DEAD® staining (Life Technologies) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... purpuratus Axin and β-catenin were cloned into pENTR using D-TOPO cloning kit (Invitrogen K240020) and recombined into CBG-tagged destination vectors via Gateway cloning (Invitrogen 11791020) ...
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bioRxiv - Developmental Biology 2024Quote: ... supplemented with 1X N-2 MAX (R&D #AR009) and Neurobasal medium (Thermo Fisher Scientific #12348017) supplemented with 1X B-27 without vitamin A (Thermo Fisher Scientific #12587010) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were further incubated for 1 h with secondary anti-rabbit-Alexa Fluor 594 antibody (1:2000; A11072, Invitrogen) or anti-mouse-Alexa Fluor 594(1:2000 ...
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bioRxiv - Genomics 2021Quote: ... first-strand cDNAs were generated from the polyA(+) RNAs using the Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific) with SSP and VN primers (supplied in the kit) ...
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bioRxiv - Microbiology 2020Quote: ... Chromatin was reverse crosslinked by incubation at 68°C for 2 h and purified by phenol:chloroform:isoamyl alcohol 25:24:1 (Life Technologies) extraction and ethanol precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... cells were lysed for RNA extraction after 48 h according to the TRIzol protocol (Thermo Fisher Scientific, Waltham, MA). cDNA was synthesized with the First Strand cDNA Synthesis Kit (New England BioLabs ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with miR-874 or a scramble miRNA mimics molecule for 48 h with RNAimax(Life Technologies) in OPTI-MEM reduced serum medium following the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated for 1 h at RT with primary antibodies as follows: rabbit anti-Tubulin (PA5-19489, Invitrogen), mouse anti-Tubulin (B-5-1-2 ...
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bioRxiv - Cell Biology 2020Quote: ... cells previously seeded overnight were incubated for 4 h with 10 μM Alexa Fluor™ 594-dextran (Invitrogen, D22913) and analysed in live by laser confocal microscopy.
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bioRxiv - Cell Biology 2020Quote: ... Donkey anti-Rabbit IgG (H+L) Secondary Antibody Alexa Fluor® 555 conjugate (Thremo Fisher Scientific, Cat No. A31572). Mouse monoclonal anti-tubulin (Anti-alpha Tubulin antibody [DM1A] - Abcam (ab7291)) ...
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bioRxiv - Immunology 2021Quote: ... and then blocked for 2 h with PBS-Tween (PBST) containing 2% (v/v) goat serum (Gibco, MD, USA). Three-fold serial dilutions of serum (starting at 1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... stained and then mounted following standard hematoxylin & eosin (H&E) and Cytoseal (Thermo Scientific™ Richard-Allan Scientific™) protocols ...
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bioRxiv - Genetics 2021Quote: cDNA was synthesized from total RNA with RevertAid H Minus Reverse Transcriptase (EP0451; Thermo Fisher Scientific, Waltham, Massachusetts, US), with Random Hexamer primers (S0142 ...
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bioRxiv - Genetics 2020Quote: ... RNA was reverse-transcribed into cDNA using the RevertAid H Minus First Strand cDNA Synthesis kit (Thermo Fisher Scientific). qPCR was performed using the Universal probe library (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... goat anti-Rabbit IgG (H+L) highly cross-adsorbed secondary antibody Alexa Fluor 488 (1/500, Invitrogen #A-11008). Cells were washed 3 x 5 min and incubated for 10 min with DAPI (1/2000 ...
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bioRxiv - Microbiology 2022Quote: ... RNA concentration was determined and was reverse transcribed using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Fisher) using a combination of random and oligo (dT ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and incubated in anti-goat or anti-rabbit IgG (H+L) Alexa 488-conjugated secondary antibodies (Invitrogen, 1:750).
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bioRxiv - Immunology 2021Quote: ... 0.1% Tween-20) and incubated for 1 h with HRP-conjugated mouse or rabbit secondary antibody solutions (Thermo Fisher) diluted in 5% milk (diluted 1/10000) ...
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bioRxiv - Immunology 2021Quote: ... the cells were then stained using 0.5 μg/ml Goat anti-mouse IgG (H+L) secondary antibody Alexa Fluor 488 (Invitrogen) diluted in PBS for one hour ...
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bioRxiv - Genetics 2021Quote: ... then incubated with donkey anti-rabbit IgG (H+L) Alexa Flour 488 (Thermo Fisher Scientific, A-21206; 1:2,000) and anti-mouse IgG (H+L ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with Alexa fluor 594 goat anti-mouse IgG (H+L) secondary antibody (Invitrogen, catalog no: A11005) or Alexa fluor 488 goat anti-rabbit IgG antibody and cells were observed under fluorescence microscope.
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bioRxiv - Plant Biology 2020Quote: ... sections were incubated for 1 h with the secondary antibody Alexa Fluor 546 goat anti-rat IgG (Life Technologies) diluted 1:1,000 in 0.1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... BN-PAGE gels were run for 2 to 2.5 h at 150 V using NativePAGETM running buffer (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Incubation with primary antibodies was conducted for 48 h in the presence of 1% BSA (Super blocker, ThermoFisher Scientific). Incubation with secondary antibodies was conducted for 24 h in the presence of 1% BSA ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorophore-conjugated secondary antibodies for ICC: Donkey anti-Mouse IgG (H+L) Alexa Fluor 594 (ThermoFisher Scientific #A-21203); Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated three times (5 min each) in permeabilization solution (0.3 % Triton X-100) before blocking for 2 h either with Normal Goat Serum (Invitrogen) in Tris-NaCl blocking buffer (1:5 ...
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bioRxiv - Cancer Biology 2021Quote: ... The relative cell viability in each well was determined after 72 h treatment using the MTT assay (Life Technologies).
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bioRxiv - Bioengineering 2021Quote: ... and reverse-transcribed into cDNA using RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, ThermoFisher Scientific, France). Presence of endogenous and/or exogenous Nix in the genome was assessed by PCR using GoTaq® Green Master Mix (M712 ...
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bioRxiv - Bioengineering 2021Quote: ... and reverse-transcribed into cDNA using RevertAid H Minus First Strand cDNA Synthesis Kit (Thermo Scientific, ThermoFisher Scientific, France). Presence of endogenous and/or exogenous Nix in the genome was assessed by PCR using GoTaq® Green Master Mix (M712 ...
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bioRxiv - Bioengineering 2021Quote: ... Secondary antibodies were Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody Alexa Fluor 647 (Thermofisher, A-21235) or Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Bioengineering 2021Quote: ... combined with the secondary antibody Alexa Fluor® 594 goat anti-Rabbit IgG (H+L) (Molecular probes, 1:500). Samples were washed with PBS (five minutes ×3 ...