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Citations for Thermo Fisher :
3601 - 3650 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Transfection of plasmid DNA was performed using Lipofectamine 2000 (Thermo Fisher Scientific) and siRNA using RNAiMAX or Oligofectamine transfection reagent (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2021Quote: ... Plasmid transfection was accomplished with Lipofectamine 2000 according to manufacturer’s protocols (Invitrogen).
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bioRxiv - Biochemistry 2021Quote: ... Plasmids were cloned and propagated in Mach I cells (Thermo Fisher Scientific) with the exception of pHelper ...
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bioRxiv - Bioengineering 2021Quote: ... Lentiviral plasmid was transfected along with the ViraPower lentiviral expression system (ThermoFisher) following the recommended protocol from the vendor ...
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bioRxiv - Immunology 2021Quote: ... All expression plasmids were transiently transfected into Expi293F cells (Thermo Fisher Scientific) using ExpiFectamine 293 transfection reagent (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Each plasmid was transformed into One Shot Stbl3 chemically competent cells (Invitrogen) and purified by ZymoPURE plasmid Maxiprep kit (Zymo research) ...
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bioRxiv - Cell Biology 2020Quote: A plasmid expressing H2B-GFP was obtained from Addgene (plasmid #11680) and transfected using the Neon transfection system (Invitrogen, Carlsbad, CA) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Plasmids or siRNA were transfected into cells by using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2020Quote: ... MDCK cells were transfected with the pCABSD-M2 plasmid using lipofectamine (Invitrogen). Cells stably expressing M2 were selected in growth medium containing 1 μg/ml blasticidin and were enriched for high M2 expression by staining with an anti-M2 antibody (clone 14C2 ...
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bioRxiv - Immunology 2021Quote: ... and respective vector plasmids in Opti-MEM and FreeStyle 293 medium (Gibco) supplemented with 3mM valproic acid ...
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bioRxiv - Microbiology 2021Quote: ... Cells were transiently transfected with expression plasmids using Lipofectamine 3000 (Life Technologies), washed twice with D-Hanks solution 6 h post-transfection ...
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bioRxiv - Immunology 2020Quote: ... miniprep purification was using PureLink™ Quick Plasmid Miniprep Kit (ThermoFisher, USA). S-gene integration check into vector was performed via restriction digestion using XbaI (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with the ViraPower plasmid mix (cat# K4970-00, Invitrogen) together with the lentiviral vectors using PolyFect transfection reagent (cat# 301105 ...
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bioRxiv - Immunology 2021Quote: ... pseudotyping- and helper-plasmid (pVSVG and psPAX2) using lipofectamine 2000 (Thermo Fisher). For over-expression ...
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bioRxiv - Immunology 2021Quote: ... All plasmids were subsequently quantified using UV spectrophotometry (NanoDrop™ -Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... with 0.2 μg plasmid (total) and 0.5 μl lipofectamine 2000 (Life technologies) and seeded in the centre of a 25-mm poly-L-lysine-coated coverslip ...
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bioRxiv - Genomics 2021Quote: ... The plasmid was transfected into WT Ngn2-iPSCs using Lipofectamine Stem (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... and a plasmid encoding for GFP using Lipofectamine Stem Transfection Reagent (ThermoFisher) following the manufacturers’ instructions (12 µL of 1 µM HiFi Cas9 + 12 µL of 1 µM sgRNA in 76 µL OptiMEM combined with 4 µL Lipofectamine Stem in 100 µL OptiMEM) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were transfected with plasmid DNA or siRNA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... the final plasmid was formed by LR reaction (LR Clonase II, Invitrogen) to recombine plasmids p5E-UAS (Tol2kit) ...
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bioRxiv - Cell Biology 2022Quote: ... packaging plasmids in 600 μL of Opti-MEM (Life Technologies; 31985-070) and 48 μL of Polyethylenimine ‘Max’ MW 40,000 Da (Polysciences ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then transfected with the plasmid using Lipofectamine® 2000 (Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Plasmids were diluted to 20 μg/mL by cold OptiPRO medium (Gibco) and transfected into the ExpiCHO-S cells 17 h later according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 0.5 μg frataxin plasmid using Lipofectamine 3000 (Invitrogen) and 36 hours later harvested for Western blot analysis ...
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bioRxiv - Biophysics 2022Quote: ... and pCMV-VSV-G envelope plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... GFP from the corresponding linearized plasmids using the mMessage mMachine kit (Ambion) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293A cells were transfected with the plasmids using Lipofectamine 3000 reagent (Invitrogen), whereas MIN6 and primary pancreatic beta cells were infected with adenoviruses.
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bioRxiv - Cell Biology 2022Quote: ... Transfection of plasmid DNA was performed using Lipofectamine 2000 (Thermo Fisher # 12566014) at a ratio of 4:1 (Lipofectamine 2000/nucleic acid (μg)) ...
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bioRxiv - Cell Biology 2022Quote: ... Transfection of plasmid DNA was performed with Lipofectamine 3000 (Invitrogen / Life Technologies) according to the manufacturer’s instructions except that cells were transfected for 6 h before fresh medium was added ...
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bioRxiv - Cell Biology 2022Quote: ... Transfection of plasmid DNA was performed with Lipofectamine 3000 (Invitrogen / Life Technologies) according to the manufacturer’s instructions except that cells were transfected for 6 h before fresh medium was added ...
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bioRxiv - Immunology 2022Quote: ... transfection of the respective plasmids was done with lipofectamine 2000 (Life technologies) in DMEM ...
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bioRxiv - Microbiology 2022Quote: Plasmid DNA (0.8 μg in 50 μl of OPTI-MEM (Life Technologies) mixed with polyethylenimine “Max” (MW ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The plasmid concentration was evaluated using a Nanodrop 2000 from Thermo Scientific.
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bioRxiv - Biochemistry 2022Quote: ... These plasmid mixtures were transfected into FreeStyle 293-F cells (Thermo Fisher) using polyethylenimine ...
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bioRxiv - Microbiology 2022Quote: ... The plasmid mini-prep and gel extraction kits were purchased from Invitrogen. S ...
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bioRxiv - Microbiology 2024Quote: ... All constructed plasmids were transformed into Stbl3 (Thermo Fisher Scientific, Cat# C737303) or Trans5α (TransGen ...
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bioRxiv - Molecular Biology 2022Quote: ... The plasmid DNA samples were quantified using Qubit® 3.0 Fluorometer (Invitrogen) following manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... All plasmids were recombined by the addition of LR Clonase II (Invitrogen) and were incubated overnight at 25°C following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Heavy and light chain plasmids were co-transfected into Expi293F cells (Gibco) for expression ...
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bioRxiv - Molecular Biology 2024Quote: ... transfection (2μg plasmid) followed by 500 μg/ml hygromycin B (Gibco, 10687010) selection for 10 days before being single-cell sorted ...
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bioRxiv - Cell Biology 2024Quote: ... and control plasmids was carried out using Lipofectamine 3000 (Thermo Fisher Scientific). All transfections were performed following the manufacturer’s protocols ...
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bioRxiv - Bioengineering 2024Quote: ... The expression plasmid was then transformed into Escherichia coli BL21lDE3 Star (Thermofisher) for use in protein production ...
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bioRxiv - Cancer Biology 2024Quote: ... ECA-109 cells were transfected with the plasmids using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Transfections of plasmids and siRNA were performed with polyethylenimine and Oligofectamine (Invitrogen) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA transfection was done using Lipofectamine 2000 (11668030; Thermo Fisher Scientific) as previously described (43) ...
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bioRxiv - Neuroscience 2024Quote: ... We then introduced the transformed plasmids into MegaX DH10 electrocompetent cells (Invitrogen). Plasmids were subsequently extracted using the Qiagen Plasmid Plus Giga Kit ...
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bioRxiv - Neuroscience 2023Quote: ... subcloned into expression plasmids by Gibson assembly (e.g. pcDNA3.1, ThermoFisher, Waltham, MA), and sequenced by Sanger sequencing and/or Oxford nanopore long-read technology using Plasmidsaurus (Eugene ...
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bioRxiv - Cell Biology 2023Quote: ... along with 10 μg lentiviral plasmid with lipofectamine 3000 (Thermo Fisher Scientific). After 15 hours ...
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bioRxiv - Cell Biology 2024Quote: Plasmid transfections were performed using Lipofectamine 3000 (Thermo Fisher Scientific, no. L3000015) for INS-1 cell lines ...
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bioRxiv - Developmental Biology 2023Quote: ... and transfected with indicated plasmids using Lipofectamine 2000 (11668019, Thermo Fisher Scientific). After 24 h ...