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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... 10 μg of each CHIKV plasmid was linearized overnight using NotI (Invitrogen), purified by phenol:chloroform extraction and used as a template for in vitro transcription using the SP6 mMessage mMachine kit (Invitrogen) ...
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bioRxiv - Plant Biology 2021Quote: ... Plasmids were transformed into Agrobacterium tumefaciens GV3201 (Thermo Scientific, Waltham, MA, US) and selected using 50 mg/L spectinomycin and 50 mg/L gentamycin to maintain the pMP90 plasmid (MP Biomedicals Inc. ...
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bioRxiv - Molecular Biology 2021Quote: ... The ATRX plasmids were propagated using ElectroMAXTM Stbl4TM Competent cells (Life Technologies). To generate Flag-tagged DAXX expression plasmid (pCDH-Flag-DAXX-Puro) ...
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bioRxiv - Cell Biology 2021Quote: ... plasmid DNA transfection was performed with the LT-X transfection reagent (Invitrogen). Transfected cells were plated on poly-D-lysine (Sigma-Aldrich)−coated borosilicate cover glass (Paul Marienfeld ...
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bioRxiv - Genetics 2021Quote: ... The plasmids were used to generate Flp-In HEK293 (Thermofisher, cat. #R78007) stable cell lines ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were transfected using Lipofectamine 2000 according to the manufacturer’s instructions (Invitrogen).
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bioRxiv - Molecular Biology 2020Quote: ... and 1 μg of pUC19-piggyBAC transposase plasmid using Lipofectamine 3000 (Invitrogen) according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... or pcDNA3.2/V5-DEST (control) plasmid using Lipofectamine2000 (Thermo Fisher Scientific, #11668500) After 6 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transfected with plasmid DNA using Lipofectamine 2000 (Thermo Fisher Scientific) following the manufacturer’s recommendations.
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bioRxiv - Cell Biology 2021Quote: Transient transfections of reporter plasmids were carried out using Lipofectamine 3000 (Invitrogen) when co-transfections with miRNA mimics or plasmids for ectopic expression of hMOV10 were assayed for retrotransposition ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cells were resuspended in 200 μL GeneJET Plasmid MiniPrep (ThermoFisher Scientific) resuspension buffer supplemented with 3 μL 1000 U/mL Zymolase (Amsbio ...
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bioRxiv - Cell Biology 2022Quote: ... The dual expression plasmid was transformed into the Rosetta II (DE3) (Invitrogen) E ...
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bioRxiv - Cancer Biology 2022Quote: ... All plasmids were grown up in Top10 competent bacteria (Invitrogen, Waltham, MA) and plasmid DNA prepped from bacteria with Qiagen DNA mini prep or maxi prep kits Germantown ...
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bioRxiv - Molecular Biology 2022Quote: ... 1-2 μg of Plasmid DNA along with transfection agents Lipofectamine (Invitrogen) or Polyetheleneimine ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmid construction and amplification were performed in the bacteria strain TOP10 (Thermofisher Scientific ...
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bioRxiv - Biophysics 2022Quote: Cells were transfected with plasmid DNA using Lipofectamine 3000 reagent (Thermofisher #L3000001) transiently or stably ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmid DNA (1 µg/ml culture) and ExpiFectamine™ 293 Reagent (Gibco) were diluted in Opti-MEM® I medium (Gibco ...
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bioRxiv - Molecular Biology 2019Quote: ... and purified using PureLink HQ Mini Plasmid DNA (Life Technologies Ltd, K210001).
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bioRxiv - Biophysics 2019Quote: ... plasmids were obtained from the GeneArt Gene Synthesis Service (Thermo Fisher Scientific). Template DNA and primer design were performed with assistance of the molecular biology module of the Benchling platform.
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were transfected with plasmid encoding ACE2 with Lipofectamine 2000 (Invitrogen). 48 h post-transfection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coli (DH5α) with an endotoxin-free plasmid extraction kit (Invitrogen, Carlsbad, CA) and transfected into ExpiCHO-S cells using ExpiFectamine Transfection Kit (Gibco) ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Amplicons containing the attB sites were recombined into pDONR 221 plasmids (Invitrogen). They were then transferred into the binary vector pGWB415 (Nakagawa et al. ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Amplicons containing the attB sites were recombined into pDONR 221 plasmids (Invitrogen). They were then transferred into the binary vector pK7FWG2 (Karimi et al. ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... Amplicons containing the attB sites were recombined into pDONR Zeo plasmids (Invitrogen). They were then transferred into the binary vector pK7WGF2 ...
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bioRxiv - Microbiology 2020Quote: The resulting plasmid pBSJL2-cat was digested by Fastdigest BamHI (Thermo scientific) and the band obtained was purified from agarose gel using QIAquick gel extraction kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... The recombinant plasmids were transformed into INVSc1 Saccharomyces cerevisiae (Thermo Fisher Scientific) using the PEG-LiAc method ...
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bioRxiv - Cancer Biology 2021Quote: Transfection of plasmids was carried out using Lipofectamin 3000 (Invitrogen Life Technologies) according to the manufacturer’s user guides ...
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bioRxiv - Cancer Biology 2021Quote: Transfection of plasmids was carried out using Lipofectamin 3000 (Invitrogen Life Technologies) according to the manufacturer’s user guides ...
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bioRxiv - Molecular Biology 2021Quote: ... the CoV2-FSE119 plasmid was transfected using lipofectamine 3000 (Thermo Fisher Scientific) at 100 ng per well ...
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bioRxiv - Microbiology 2020Quote: ... and then inserted to pTH1 plasmid using T4 ligase (Thermo Fisher Scientific) to yield the pLJ-Ssc70-Myc plasmid ...
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bioRxiv - Microbiology 2020Quote: ... plasmid DNA was transfected into 293FT cells using Lipofectamine (Life Technologies, UK) or Fugene 6 (Promega ...
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bioRxiv - Synthetic Biology 2020Quote: ... 1µg of this plasmid was digested with FastDigest SgsI (FD1894, Fisher Scientific), transformed into KmASR.039 and selected on SD-ura agar ...
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bioRxiv - Cancer Biology 2020Quote: ... cells at 70% confluence were transfected with plasmids by using Lipofectamine3000 (Invitrogen) in serum-free medium according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with VAPA-EGFP plasmid DNA using Lipofectamine 2000 (Invitrogen). On the day of imaging ...
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bioRxiv - Genomics 2021Quote: ... The respective plasmid was then transfected with Lipofectamin 2000 (Thermo Fisher Scientific) into female TX1072 mESCs in a 5-to-1 transposase-to-target ratio with the hyperactive transposase (pBroad3_hyPBase_IRES_tagRFP ...
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bioRxiv - Immunology 2021Quote: ... Heavy and light chain plasmids were co-transfected into Expi293F cells (Gibco) for expression ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmids were transfected with Lipofectamine® 2000 Transfection Reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... Plasmids (2 µg) and transfection reagent (5 µL of Lipofectamine 2000; Invitrogen) was added to 125 µL of OptiMEM serum-free media (ThermoFisher ...
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bioRxiv - Cell Biology 2019Quote: ... Modification plasmid pWPTS-GFP was performed by Gateway Vector Conversion System (Invitrogen). A Gateway cassette containing attR recombination sites flanking ccdB gene and a chloramphenicol-resistance gene are blunt-end cloned into cloning site pWPTS-GFP to generate a pWPTS-Dest vector ...
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bioRxiv - Biochemistry 2020Quote: ... The DNA template was purified with GeneJET Plasmid Midiprep Kit (ThermoFisher Scientific) and linearized with the AarI restriction enzyme (ThermoFisher Scientific) ...
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bioRxiv - Biophysics 2019Quote: ... The ligated plasmid was transformed into MAX Efficiency Stbl2 Cells (Life Technologies) and a correct clone was identified via restriction enzyme analysis and verified by sequencing.
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bioRxiv - Synthetic Biology 2020Quote: ... the cells were transfected with plasmid constructs using Lipofectamine LTX (Thermo Fisher) as per manufacturer’s protocol.
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bioRxiv - Biochemistry 2020Quote: ... plasmids were transfected into HEK293-T cells using Lipofectamine 2000 (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were transformed into Stbl3 chemically competent Escherichia coli (Thermo Fisher Scientific) and colonies containing correct plasmids were selected by colony PCR followed by Sanger sequencing (at Fasteris ...
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bioRxiv - Genetics 2021Quote: ... All plasmid transfections were performed using Lipofectamine™ 2000 (Invitrogen, Carlsbad, CA) and Opti-MEM (Gibco ...
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bioRxiv - Genomics 2021Quote: ... the intermediate plasmid was digested by BmsbI enzyme (ThermoFisher, cat. no ER0451). After purification ...
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bioRxiv - Physiology 2021Quote: ... or PKI plasmids using Lipofectamine 2000 Transfection Reagent (Thermo Fisher Scientific, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The PCR product was then cloned in pJET2.0 plasmid (Thermo Scientific U.K.) according to the manufacturer’s instructions and named pOST9 ...
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bioRxiv - Developmental Biology 2021Quote: ... The plasmid was transformed into DH5-alpha library-efficiency competent cells (Invitrogen) through the standard heat shock protocol ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids were transfected using the ExpiFectamine 293 Transfection Kit and protocol (Gibco) 35–37 and purified by Protein A affinity ...