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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The final plasmid construct was generated by MultiSite Gateway® cloning (Invitrogen). The strain expressing HIS-1/H4-GFP was made using the MosSCI technique (42) ...
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bioRxiv - Plant Biology 2023Quote: ... The fragments were cloned into the pENTR/D-TOPO plasmid vector (Invitrogen) to obtain pYC19 (pENTR-AGB1.1 ...
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bioRxiv - Biophysics 2024Quote: ... cells were transfected with 1 μg BacMam plasmids using Lipofectamine 3000 (Invitrogen) in Opti-MEM media (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... plasmids (2 µg) were delivered using Lipofectamine® 3000 (Invitrogen, L3000-001) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... cells were transfected with plasmids using Lipofectamine 3000 (Invitrogen, cat. no. L3000150) according to the manufacturer’s protocol 12 hours post plating ...
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bioRxiv - Cancer Biology 2024Quote: ... The plasmids were mixed in the above ratio in Turbofect (Invitrogen, USA), incubated at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... and 6µg of vector plasmid in 1.5ml of Opti-MEM (Fisher Scientific) and 45µL of TransIT-LT1 (Mirus Bio ...
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bioRxiv - Biochemistry 2024Quote: ... The linear mutagenized plasmids were then phosphorylated using T4 polynucleotide kinase (Invitrogen) prior to ligation ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was purified using the PureLinkTM HiPure Plasmid Filter Maxiprep kit (Invitrogen) and equal sgRNA coverage within the library was confirmed by sequencing.
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bioRxiv - Biochemistry 2024Quote: ... Plasmid DNA (100 ng per well) was transfected using Lipofectamine 2000 (Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Genetics 2024Quote: ... or PureLinkTM HiPure Plasmid filter Maxiprep Kit (Thermo Scientific, Lafayette, CO, USA). Natural transformation was performed according to the procedure described in (27).
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bioRxiv - Microbiology 2024Quote: ... The furin fragment from this plasmid was subcloned into pcDNA3.1(–) (Life Technologies).
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bioRxiv - Neuroscience 2024Quote: ... plasmid DNA (diluted in Opti-MEM, GIBCO, Thermo Fisher Scientific, Waltham, MA) was mixed with 1.5ul of PLUS™ Reagent and incubated for 5 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2024Quote: ... plasmid DNA (diluted in Opti-MEM, GIBCO, Thermo Fisher Scientific, Waltham, MA) was mixed with 1.5ul of PLUS™ Reagent and incubated for 5 min at room temperature (RT) ...
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bioRxiv - Synthetic Biology 2021Quote: ... CD3+ T cells were activated with anti-human CD3/CD28 magnetic Dynabeads (CTS, ThermoFisher) in a 1:1 ratio with 100 U mL-1 of IL-7 and 10U mL-1 IL-15 (R&D Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... FAM-labeled target primer and endogenous human ACTB control (beta actin) (Thermo Fisher Scientific) were mixed with master mix and template ...
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bioRxiv - Microbiology 2020Quote: ... Human embryonic stem cell (hESC; H9)-derived neural stem cells (NSC) (Thermo Fisher Scientific) were cultured ...
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bioRxiv - Microbiology 2020Quote: A549 human epithelial cells (ATCC ref# CCL-185) were maintained in F12K media (Gibco) supplemented with 1x GlutaMax (Gibco ...
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bioRxiv - Cancer Biology 2021Quote: ... AlexaFluor 488 conjugated anti-human/mouse CD44 antibody (1:50 dilution, Molecular probes, A25528) was directly added into the cell culture medium of the cells to be stained ...
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bioRxiv - Cell Biology 2020Quote: ... Taqman probes targeting human GAPDH (Hs02758991_g1) and IFIT1 (Hs03027069_s1) were purchased from Life Technologies. qRT-PCR data were collected on a StepOnePlus™ Thermal Cycler (Thermo Fischer Scientific ...
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bioRxiv - Immunology 2021Quote: Membranes were coated with an anti-human IgG capture antibody (Thermo Fisher Scientific, A18813). Cells were then added to the plate in duplicate in 2 fold serial dilutions and incubated for 24hrs prior to detection using an alkaline phosphatase-linked anti-human IgG detection antibody (Jackson ...
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bioRxiv - Genetics 2021Quote: ... donkey-anti-rabbit and mouse-anti-human IgG secondary antibodies (Molecular Probes, 1:1000), or NbALFA-Atto647 (NanoTag Biotechnologies ...
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bioRxiv - Genomics 2021Quote: ... Human K562 erythroleukemia cells were cultured in Roswell Park Memorial Institute (RPMI, Life Technologies) 1640 medium supplemented with 10% FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... or of the rabbit anti-Human Rac1 polyclonal antibodies (1:40, Thermofisher PA1-091) were added in 1 % BSA-PBS and incubated O/N at 4°C ...
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CRISPR-SID: identifying EZH2 as a druggable target for desmoid tumors via in vivo dependency mappingbioRxiv - Cancer Biology 2021Quote: ... Total RNA was isolated from human desmoid tumor cell lines using Trizol reagent (Invitrogen) according to the manufacturer instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... Primed human ESC and iPSC cells were harvested with 0.25% trypsin-EDTA solution (Invitrogen), and cells resuspended in PBS were transfected with 75μg DNA constructs (Gene Pulser Xcell System ...
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bioRxiv - Microbiology 2022Quote: ... human foreskin fibroblast (HFF) monolayers were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplemented with 25 mM glucose (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 20 ng/mL human recombinant basic fibroblast growth factor (bFGF; Invitrogen, USA), 20 ng/mL epidermal growth factor (EGF ...
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bioRxiv - Molecular Biology 2021Quote: ... slides were incubated with Alexa Fluor 488 goat anti-human IgG(H+L) (Invitrogen) at 1:500 (in 3% BSA/PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... except for human XBP1S that was assessed using SYBR green master mix (Thermofisher Scientific) using primers in Supplementary Table 3.
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bioRxiv - Molecular Biology 2021Quote: ... Stable integration of CLIP-UPF1SL into human Flp-InTM T-RExTM-293 cells (Invitrogen) and subsequent maintenance of this stable line was previously described (Kishor et al ...
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bioRxiv - Genomics 2020Quote: The THP1 (human acute monocytic leukemia derived; ATCC number TIB-202 purchased from Thermofisher) cell line[18] was regularly maintained in RPMI 1640 medium containing GlutaMAX™ ...
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bioRxiv - Genomics 2020Quote: ... Samples passing quality control were analyzed using the human Clariom D Assay (ThermoFisher Scientific) and analyzed using Transcriptome Analysis Console (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... and stained with Alexa 647-conjugated anti-human IgG (H+L) antibody (Thermo Fisher) for 1h at 4°C ...
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bioRxiv - Pathology 2020Quote: ... SMCs were transfected with control siRNA or siRNA targeting the human calpain-2 (Ambion - Silencer Select validated siRNA ...
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bioRxiv - Microbiology 2019Quote: ... and 5 μg.L−1 of epidermal growth factor (EGF) human recombinant (Thermo Fisher Scientific) (Wu et al ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 human embryonic kidney cells were cultured in Dulbecco’s modified Eagle medium (DMEM, Invitrogen), containing 10% FBS ...
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bioRxiv - Biophysics 2019Quote: Homo sapiens SUR1 and human Kir6.2 were subcloned into pcDNA4/TO (Invitrogen; Carlsbad, CA) for expression in HEK-293T cells ...
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bioRxiv - Genomics 2019Quote: ... dataset (which used an Affymetrix platform, [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array); the default importer in BRB-ArrayTools was used to collate the data from .cel files ...
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bioRxiv - Immunology 2019Quote: ... anti-human CD3 conjugated to FITC (clone OKT3, cat. no. 11-0037-41, Invitrogen), anti-human CD8 conjugated to Pacific Blue (clone 3B5 ...
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bioRxiv - Immunology 2019Quote: ... Cortactin expression was assessed using rabbit anti-human cortactin antibody (PA5-27134; Life Technologies) stained in conjugation with goat anti-rabbit Cy3 (Life Technologies) ...
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bioRxiv - Genetics 2019Quote: ... cells were incubated in differentiation medium (DM) (10 µg/ml recombinant human insulin (Gibco), 2% HI horse serum (Thermo Fisher) ...
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bioRxiv - Immunology 2019Quote: ... and the ELISPOT plate was incubated with biotinylated goat anti-human IgG Fc (Invitrogen), followed by HRP-conjugated avidin (Vector Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the cells were stained with AlexFuor488-conjugated goat anti human Fc (Life technologies) at RT for 30 min and analyzed by flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: ... recombinant human insulin-like growth factor 1 (hIGF1; 100 ng/mL; Thermo Fisher Scientific), holo-transferrin (200 µg/mL ...
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bioRxiv - Genomics 2019Quote: ... We used the Genome-Wide Human SNP Array 6.0 (Affymetrix, Santa Clara, CA, USA) for genotyping single nucleotide polymorphisms (SNPs) ...
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bioRxiv - Immunology 2021Quote: ... coated with 0.5 µg/well of goat anti-human Ig polyvalent antibody (ThermoFisher, #H17000) in 0.1 M carbonate buffer ...
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bioRxiv - Immunology 2021Quote: ... Human CD8+ T cells were stimulated with CD3/CD28 activator beads (Thermo Fisher Scientific) and cultured in DMEM medium (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2021Quote: ... followed by the secondary goat anti-human HRP-conjugated antibody (1:4000, A18805, Invitrogen). Stained proteins were detected using Immobilon Western Chemiluminescent HRP Substrate (Millipore ...
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bioRxiv - Biochemistry 2020Quote: Human GCDH cDNA (clone ID 3142978) in pOTB7 vector was obtained from Thermo Fisher Scientific Open Biosystems repository (now part of Horizon Discovery) ...