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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations for Human UFL1 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: Human foreskin dermal fibroblasts were cultured in DMEM (Thermo Fisher, Cat No: 11965092) with 10% FCS and 1% Pen/Strep at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: Suspension adapted human embryonic kidney cells (HEK293F) were acquired directly from Thermo Fisher Scientific as an authenticated product maintained according to the manufacturer’s guidelines in either FreeStyle 293 Expression medium (Cat #12-338-018 ...
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bioRxiv - Cell Biology 2024Quote: ... The following Taqman probes were used: human ACTB (Thermo Fisher Scientific, 4331182-Hs01060665_g1), human MCU (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Human embryonic kidney (HEK293, ATCC #CRL-1573) cells were cultured in DMEM (Gibco), supplemented with 10% (vol/vol ...
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bioRxiv - Neuroscience 2024Quote: ... we used a pair of rabbit anti-human IgG heavy chain antibodies (Invitrogen) and HRP-conjugated goat α-human IgG kappa light chain antibodies (Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... and HRP-conjugated goat α-human IgG kappa light chain antibodies (Thermo Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... The AML sample underwent human CD3 depletion using dynabead CD3 depletion kit (Invitrogen) prior to injection ...
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bioRxiv - Cancer Biology 2024Quote: ... siRNA oligonucleotides (20 nM) targeting eIF4B (Human: s4573 & s4574; Mouse: s93804 & s93805 ThermoFisher) were transfected using Lipofectamine RNAiMax (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... Primary human T cells were cultured in AIM-V medium (Invitrogen 12055-083) supplemented with 5% Human serum (Sigma H4522-100ML) ...
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bioRxiv - Microbiology 2024Quote: Human embryonic kidney (HEK)293T (ATCC CRL-11268) and FreeStyle 293F (Life Technologies) cell lines were used for recombinant protein productions ...
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bioRxiv - Immunology 2024Quote: Human serum derived macrophages were lifted using TrypLE (5 mL, Thermo Fisher Scientific). Once lifted ...
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bioRxiv - Cell Biology 2024Quote: ... and a human proteome database (UniProt) was screened with SEQUEST (Thermo Fisher Scientific). The normalization node was added to the processing workflow ...
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bioRxiv - Cancer Biology 2024Quote: ... A549 human lung adenocarcinoma cells (CCL_185, ATCC) were cultured in DMEM (Gibco, #41965039), and supplemented with 10% FBS (Gibco ...
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bioRxiv - Microbiology 2024Quote: ... human stool samples were diluted 1:10 in DEPC-treated water (Life Technologies), vortexed ...
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bioRxiv - Immunology 2024Quote: ... IL-1β was evaluated using an IL-1 beta Human ELISA Kit (Invitrogen). Neutrophils (3 × 105/well ...
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bioRxiv - Immunology 2024Quote: ... ELISA plates were coated with anti-human IgG (ThermoFisher 88-50550-88, 1X) and blocked overnight with 2X assay buffer (Invitrogen 88-50550-88 ...
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bioRxiv - Molecular Biology 2024Quote: ... the CECs were resuspended in stabilisation media (containing Human Endothelial-SFM (Life technologies) supplemented with 5% foetal bovine serum (FBS) ...
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bioRxiv - Plant Biology 2020Quote: Plasmids were extracted from bacterial cultures with a Midi Prep kit (Invitrogen). One ug/ul of each plasmid was introduced into Arabidopsis protoplasts derived from four-weeks old plants by polyethyene glycol-mediated transfection and incubated overnight at room temperature (Fujikura ...
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bioRxiv - Cancer Biology 2021Quote: Plasmid transfection was carried out using Lipofectamine™ 3000 (Invitrogen™ 11668019) or ViaFect™ (Promega™ E4981 ...
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bioRxiv - Cancer Biology 2021Quote: Cell transfection was performed with plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2021Quote: All plasmids were transfected with Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific) following manufacturer’s instructions ...
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Base editing in bovine embryos reveals a species-specific role of SOX2 in regulation of pluripotencybioRxiv - Developmental Biology 2021Quote: ... the plasmid underwent in vitro transcription using mMESSEAGE mMACHINE T7 kit (Invitrogen) and were purified by LiCl precipitation ...
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bioRxiv - Cell Biology 2020Quote: ... 1-2 μg of Plasmid DNA along with transfection agents Lipofectamine (Invitrogen) or Plyetheleneimoine ...
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bioRxiv - Cell Biology 2019Quote: ... cloning the PCR amplicons in the pCR2.1 TOPO plasmid (Thermo Fisher Scientific) and transforming 10-beta competent E ...
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bioRxiv - Cell Biology 2020Quote: Transfection of plasmids was performed using Lipofectamine 2000 reagent (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 3.75 µg of each plasmid and 3.75 µl PLUS reagent (Invitrogen 15338100) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... coli and extracted using the GeneJET Plasmid Miniprep Kit (Thermo Scientific™). The construction was verified using enzymatic digestion with KpnI and PvuI.
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bioRxiv - Evolutionary Biology 2021Quote: ... coli cultures using PureLink HiPure Plasmid Midiprep Kit (Invitrogen, Cat. No. K210005). To facilitate DNA delivery to ESCs through nucleofection ...
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bioRxiv - Plant Biology 2022Quote: ... the linearized plasmid was digested with 20 U DNase I (Thermo Scientific) for 10 min at 37°C ...
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bioRxiv - Plant Biology 2022Quote: ... Plasmid assembly was performed by ligation with T4 DNA ligase (Thermo Scientific) or by the Gibson reaction using the NEBuilder HiFi assembly master mix (New England Biolabs) ...
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bioRxiv - Microbiology 2022Quote: ... and 2.5 μg pVSV-G plasmid with 10 μL Lipofectamine 2000 (Invitrogen) diluted in 4 mL Opti-MEM ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... The plasmids were transfected with Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA) and siRNAs transfected with RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Positive clones were confirmed by plasmid sequencing (3130 Genetic Analyzer, Applied Biosystems). Resulting sequences were analyzed using Sequence Scanner v 2.0 (Applied Biosystems) ...
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Capacity to erase gene occlusion is a defining feature distinguishing naive from primed pluripotencybioRxiv - Molecular Biology 2021Quote: ... PCR products were cloned into plasmid using TOPO TA Cloning Kit (Invitrogen) and individual clones were picked for Sanger sequencing of the inserts.
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bioRxiv - Molecular Biology 2020Quote: ... plasmid DNA was mixed with calf thymus carrier DNA (Thermo Fisher Scientific) to give a total of 10 μg DNA ...
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bioRxiv - Cell Biology 2022Quote: Plasmids were pre-complexed with 3 µg lipofectamine 2000 (Life Technologies 11668019) in 200 µl Opti-MEM (Life Technologies 51985091 ...
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Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesisbioRxiv - Evolutionary Biology 2022Quote: ... The entire construct was cloned into a pJet1.2 plasmid backbone (Thermo Fisher) using Gibson assembly master mix (NEB)79 ...
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bioRxiv - Synthetic Biology 2020Quote: ... cultures were prepared using a GeneJET Plasmid Miniprep Kit (Thermo Fisher Scientific). Part plasmids were verified by sequencing (Elim Biopharmaceuticals ...
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bioRxiv - Cancer Biology 2019Quote: ... The plasmid DNA/siRNA and lipofectamine were individually diluted in Optimem (Gibco) and incubated for 10 minutes at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... and GeneJet gel or plasmid purification kits were purchased from Thermo Scientific. Endotoxin-free plasmid DNA isolation kits were purchased from Qiagen (cat ...
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bioRxiv - Molecular Biology 2019Quote: The Eco72IM sequence was PCR-amplified from the Eco72IRM plasmid (Thermo Fisher) and recombined with the pDONR 221 (Gateway ...
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bioRxiv - Developmental Biology 2020Quote: ... HEK293T cells were transfected with indicated plasmids using Lipofectamine 2000 (Life Technologies). After 48h ...
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bioRxiv - Molecular Biology 2019Quote: ... plated and extracted using GeneJET Plasmid Midiprep Kit (Thermo Fisher Scientific, USA). Number of colonies was estimated to be around 10000 individual clones.
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bioRxiv - Genetics 2019Quote: ... Plasmids were transfected into podocytes or HEK293 cells using Lipofectamine 2000 (Invitrogen). HEK293 cells stably expressing p3xFLAG-ADCK4 or BAP were selected and maintained with 1 mg/mL G418.
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bioRxiv - Molecular Biology 2019Quote: Three incisions-bearing plasmid (∼10 μg) was column purified (Invitrogen, cat#K220001) to check for digestion ...
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bioRxiv - Genomics 2020Quote: ... G8P plasmid (1 μg) were transfected into cells using Lipofectamine 3000 (Invitrogen). At 24 h post G8P transfection ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmid transfections and siRNA knock-downs were done using Lipofectamine 3000 (Invitrogen) in antibiotic-free media ...
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bioRxiv - Molecular Biology 2019Quote: ... and the template plasmid was digested with Turbo DNase (Thermo Fisher #AM2239). Transcribed mRNAs were purified LiCl precipitation and were used for injection ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids containing PSHCP were transformed into BL21 (DE3) star cells (Invitrogen, C601003). PSHCP mutants were generated using Q5 Site-Directed Mutagenesis Kit (NEB ...
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bioRxiv - Cancer Biology 2021Quote: ... HaCaT cells were transfected with plasmid minigene and selected using G418 (Gibco). Expression and splicing of the minigene was assessed by RT-qPCR using primers presented in the reagent table.