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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... at 6 DIV with a total of 500ng DNA and 1.0 μl Lipofectamine 2000 reagent per well (11668019; ThermoFisher Scientific) in Opti-MEM reduced serum medium (31985062 ...
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bioRxiv - Neuroscience 2024Quote: ... The slices were finally placed on 0.4 mm PTFE cell culture inserts (Millicell) equilibrated in a 6-well plate with serum-free media containing 1x B27 supplement (Gibco), 1x N2 supplement (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... We performed reaction in triplicates with 10 µl total volume using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). We normalized qPCR signal from ChIP DNA by 2% DNA input using the formula % of input = 100*2ᶺ(Ct [input]-Log2(50 ...
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bioRxiv - Immunology 2024Quote: ... BHK-21 cells (7×105/well) were transfected in tissue culture-treated 6-well plates using Lipofectamine 2000 (ThermoFisher Scientific) with the LCMV Arm plasmids pCAGGS-NP (0.8 µg) ...
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bioRxiv - Immunology 2024Quote: ... The amount of TNF-α and IL-6 in serum was quantified using commercial mouse ELISA kits (Thermo Fisher Scientific). For ex vivo experiments using splenic myeloid cells ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated into 6-well plates and transfected at ∼75% confluency with1 μg of DNA using lipofectamine 3000 (ThermoFisher). For imaging ...
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bioRxiv - Developmental Biology 2023Quote: ... The precipitated pellets containing the cumulus-oocyte complexes (COCs) were transferred to a 9 cm Petri dish and 6 ml of wash medium (TCM 199 - GIBCO, buffered with 2.5% HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µg DNA was mixed with 6 µL of FuGENE HD in 100 µL of Opti-MEM (Thermo Fisher Scientific) and incubated at RT for 10 min before being added to HeLa cells grown in 35-mm glass-bottomed dishes (AGC Technology Solutions ...
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bioRxiv - Cell Biology 2023Quote: ... which were blotted with a force of 6 for 5 seconds and immediately vitrified by plunge-freezing in liquid ethane using a Vitrobot Mark IV (ThermoFisher) operating at 4 °C and 100% humidity ...
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bioRxiv - Cell Biology 2023Quote: U2OS cells were transfected with 2.5 μg of plasmid per well (6 well plate) at approximately 80% confluence using Lipofectamine 2000 (ThermoFisher, 11668019) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... ISE6 cells were fixed with 4% paraformaldehyde followed by 1 x PBS washes and the coverslip was mounted on a slide using Antifade gold mounting reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen) and observed under the Nikon W-1 Spinning Disk confocal microscope ...
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bioRxiv - Developmental Biology 2023Quote: ... were maintained on gelatin-coated culture plates (6-well) in N2B27 medium consisting of 50 % DMEM/F12 (Catalog no. 21331020, Gibco) and 50 % Neurobasal medium (Catalog no ...
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bioRxiv - Immunology 2023Quote: ... The reaction was then rapid cooled down on ice and 6 μL of 5x FS Buffer (Invitrogen, cat. no: 18080044), 1.5 μL of 0.1 M DTT (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... 1 µg of plasmid was used to transfect 750,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 µL; Thermo Fisher) in OptiMEM (400 µL ...
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bioRxiv - Neuroscience 2023Quote: ... were grown to 80% confluence in 6-well culture plates and maintained in Dulbecco’s modified Eagle medium (DMEM, Gibco, USA) with 10% fetal bovine serum (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... the sections were mounted with ProLong Gold with 4′,6-diamidino-2-phenylindole (DAPI) (catalog no. P36935, Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... Cells were then washed twice in FACS buffer and analyzed by flow cytometry on an Attune NxT 6 cytometer (ThermoFisher). Antibodies used are shown in Supplemental Methods ...
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bioRxiv - Molecular Biology 2023Quote: Whole cell extracts were resolved using a Novex™ WedgeWell™ 6% Tris-Glycine Mini Protein Gel (Thermo Fisher XP00065BOX). Samples were transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... the hippocampus form 6-8 neonatal mice was collected from both hemispheres in Hank’s buffered saline solution (HBSS, Life Technologies). Tissue was incubated in 0.25% trypsin (Life Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Human intestinal epithelial cells (HIEC-6) were cultured in Opti-MEM™ I Reduced Serum Medium (Thermo Scientific, MA, USA). Cell viability was measured using MTT assays (ref) ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
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bioRxiv - Neuroscience 2023Quote: ... neurons were dissociated and seeded (on coverslips inside a 6 well plate: 0.2E6 cells/well) onto plates containing NB plus [Neurobasal medium supplemented with B27 (Invitrogen GIBCO Life Technologies), GlutaMAX (GIBCO) ...
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bioRxiv - Molecular Biology 2023Quote: ... The DNA samples were amplified in quadruplicate reactions as previously described100 using the QuantStudio 6 Flex RealTime PCR system (Applied Biosystems) using the PowerUP SYBR Green Master Mix (Applied Biosystems).
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bioRxiv - Pathology 2023Quote: ... The samples were then incubated for 24 hours at 37°C in a standardised volume (6 mL/g of tissue) of RPMI 1640 medium (Gibco) supplemented with antibiotics and antimycotics ...
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bioRxiv - Biophysics 2023Quote: The membrane probes Di-4-ANEPPDHQ and Laurdan (6-dodecanyl-2-dimethylaminonaphtalene) were obtained from Molecular Probes (Eugene, OR, USA) and dissolved in ethanol and dimethyl sulfoxide (DMSO ...
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bioRxiv - Cancer Biology 2023Quote: Rhodamine tubulin was generated by labeling tubulin with X-rhodamine SE (5(and-6) carboxy-X-rhodamine succinimidyl ester) dye (ThermoFisher). Labeling stoichiometry was determined using a molar extinction coefficient of 115,000 M-1 cm-1 for tubulin and 78,000 M-1 cm-1 for X-rhodamine [58] ...
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bioRxiv - Molecular Biology 2023Quote: ... from one full NGM agar plate or from one well from 6 well plates and total RNA was isolated with Trizol (Invitrogen). DNAse treatment was achieved using DNA-freeTM ...
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bioRxiv - Biochemistry 2023Quote: The cells were seeded in 6 well plate and treated the next day using 2 µg of plasmid and 4 µL of lipofectamine 3000 (Invitrogen) for 24 h or 10 nM of siRNA and 5 µL of RNAiMAX (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme) on the QuantStudio 6 Flex (Life Technologies). Briefly ...
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bioRxiv - Cancer Biology 2023Quote: EMT-6 tumors were analyzed on a Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Scientific, Waltham, MA, USA) with heated electrospray ionization (HESI ...
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bioRxiv - Developmental Biology 2023Quote: MAN13 hESCs were expanded in 6-well cell culture plates previously coated with 5µg/ml Human Recombinant Vitronectin (Gibco, #A27940) in Dulbecco’s Phosphate Buffered Saline (PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 100-500 ng of gel-purified PCR products were transfected into 500,000 HCT116 cells plated the day before in a well of a 6-wells plate using Lipofectamine 3000 (Thermo Fisher). Two hours later ...
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bioRxiv - Neuroscience 2023Quote: ... biopsies were cut in small pieces containing all skin layers and plated on a 6-well plate coated with 0.1% gelatin with fibroblast extraction media (FEM; DMEM (Thermo Fisher), 20% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: The fragment size distribution during TMZ selection was assessed using a 6-carboxyfluorescein-labeled reverse primer in a PCR assay and a GA3500 genetic analyzer (ThermoFisher).
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bioRxiv - Cancer Biology 2023Quote: ... 3×106 MART-1-transduced T cells were added and incubated for the indicated hours (2, 4 or 6) in “Intermediate” 4K6R medium consisting of RPMI 1640 Medium for SILAC (88365, Thermofisher) with 10% dialyzed fetal bovine serum (15605639 ...
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bioRxiv - Cancer Biology 2023Quote: ... a 6-well plate of cells at approximately 80-90% confluency was harvested by treatment with trypsin without EDTA (Gibco). The staining was performed by the use of an Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme) ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were plated in 6 well plates at 8 x 10^5 cells / well with DMEM / 10% FBS / NEAA / Pen-Strep (Gibco). Cells growing at ∼70-80% confluency were transfected with 1 μg of the desired expression plasmid (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... or 5’-TCAAGGTCCCTGATAATTATGGCGA-3’) or scrambled siRNA (non-targeting control) using 6 μL Lipofectamine™ RNAiMAX (Invitrogen™ - Cat.# 13778075). After 24 h ...
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bioRxiv - Genetics 2023Quote: ... surviving cells were passaged on Matrigel-coated 6 well plates at a concentration of 500K cells/well in neural differentiation medium {Neurobasal medium (Gibco), B27 supplement (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: Day 6 moDCs were transfected with GFP-WT cofilin or mCherry-labelled mutant cofilin using a Neon electroporator (Thermofisher Scientific). moDCs were pulsed twice at 1000 V for 40 ms and using 10 µg of the corresponding plasmid ...
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bioRxiv - Cell Biology 2023Quote: U2OS were transfected with the MAC-tagged GTPBP8 expression vector or a control MAC-tagged GFP vector using Fugene 6 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... then incubated with CaR buffer containing either 2 mM CaCl2 or 2 mM EDTA in the absence or presence of 6 mM MβCD (Thermo Fisher). For monitoring PS exposure and dextran permeability ...
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bioRxiv - Developmental Biology 2023Quote: ... Pax9 was cloned from 24 hpf cDNA using the primers pax9F 5’-TCTAGAATGGAGCCAGCCTTT-3’ and pax9R 5’-ATGGATCCTCATAGAGCTGAAGCCACCAG-3’ (Supplementary Table 6) and cloned by TOPO-TA to the pCRII vector (Invitrogen) to create pCRII pax9 ...
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen). After 6-8h transfection ...
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bioRxiv - Microbiology 2023Quote: Cells incubated in 6-well plates for 48 h following transfection were lysed using 750 μl of TRIzol (Life Technologies). RNA extraction was performed using the miRNeasy® mini kit (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR was performed on generated cDNA with SYBR Green using QuantStudio 6 Real-Time PCR (ThermoFisher Scientific, Australia). Forward and reverse prime sequences for each gene of interest are shown in Table 7 ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were washed a further two times in wash buffer before incubation with IL-6-PE (12-7069-82, Invitrogen) and IL-8 FITC (BH0814 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Peptides were eluted along an optimized 90 min gradient from 6 to 40% Mixture B (80% ACN/0.5% formic acid) with an EASY-nLC 1,200 system (Thermo Fisher Scientific). Spray voltage was set to 2.2 kV ...
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bioRxiv - Biophysics 2023Quote: ... Adherent semiconfluent HEK293SG cells cultured in DMEM:F12 medium supplemented with 10% FBS were transfected in 6-well plates with 2 μg/well of plasmid DNA complexed with Lipofectamine 2000 reagent (Invitrogen). The cells were incubated for 48 hrs at 37 °C under 7% CO2 ...
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bioRxiv - Developmental Biology 2023Quote: ... of at least 6 were used for cDNA synthesis with the SuperScriptTM First-Strand Synthesis SuperMix for qRT-PCR (Invitrogen). Real-time PCR was conducted using PowerUpTM SYBRTM Green Master mix (Applied Biosystems ...