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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Cell culture dimensionality influences mesenchymal stem cell fate through cadherin-2 and cadherin-11bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used were rabbit polyclonal anti-cadherin-11 and mouse monoclonal anti-cadherin-2 (3B9) (both 1:100; Thermo Fisher Scientific). Secondary antibodies used were goat anti-mouse Alexa Fluor 488 and goat anti-rabbit Alexa Fluor 647 (both 1:500 ...
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bioRxiv - Cell Biology 2019Quote: ... To detect protein levels by inmmunoblotting a iBind system was used and the following antibodies were used: ZO1 mouse monoclonal (1:750, Invitrogen, 33-9100), ZO2 polyclonal rabbit (1:750 ...
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bioRxiv - Cell Biology 2021Quote: ... The plates were then incubated with 100 µL concentration in each well mouse anti-alpaca monoclonal antibody (1:2000; Thermo Fisher Scientific) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... monoclonal antibody and further stained with Hoechst (1:10000) and appropriate Alexa Fluor (488/594/647)-conjugated secondary antibodies (Thermo Fisher Scientific). The stained plates were imaged using an automated high-content spinning-disk microscope CQ1 (Confocal Quantitative Image Cytometer ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% PFA for 30 minutes and stained for intracellular ENV with 1A1D-2 (1/500) monoclonal antibody and anti-mouse 647-conjugated antibody (2 μg/ml, Invitrogen Cat# A21235).
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bioRxiv - Cancer Biology 2019Quote: ... The blot was blocked for 1 h with 5% skimmed milk and the membrane was probed with anti-PLK1 primary monoclonal antibody (Invitrogen: 37-7000), anti-BCL2 primary monoclonal antibody (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... Caveolin-1 rabbit polyclonal antibody (# PA1-064) and anti-pFAK (Y397) rabbit monoclonal antibody (# 44624) obtained from Thermo Fisher (Grand Island, NY).
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bioRxiv - Neuroscience 2021Quote: ... a series of every 6th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with rabbit anti-GFP monoclonal antibody (1:500, G10362, Thermo Fisher Scientific) and rabbit anti-mKO polyclonal antibody (1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... but the immunolabeling was carried out with mouse monoclonal anti-LAMP-1 antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific; RRID:AB_2016657) or with corresponding isotype control (RRID:AB_470230).
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bioRxiv - Physiology 2020Quote: ... Membranes were blocked with casein before primary antibodies were added (1:3000 dilution of mouse monoclonal antibody against the V5 tag (ThermoFisher MA5-15253)) in a casein buffer containing Tween ...
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bioRxiv - Microbiology 2021Quote: ... Samples were fixed with 4% buffered paraformaldehyde at 24 hpi and stained with Monoclonal anti-Flag M2 antibody (1:500, Sigma, Thermo Scientific™) and alexa 488 anti-mouse secondary antibody to visualize expressing Chlamydia ...
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bioRxiv - Immunology 2022Quote: ... or monoclonal antibodies (final concentration of 100 μg/ml) in RPMI 1640 media supplemented with 10% FBS 1% Pen/Strep (Gibco, Gaithersburg, MD) for 1 hour at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... and once with PBS followed by incubation with monoclonal Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (dilution 1:1000; Molecular Probes, USA) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The binding of PfPHB1 and PfPHB2 primary antibodies was detected by incubating the samples with monoclonal Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (dilution 1:1000; Molecular Probes, USA) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: The following primary antibodies were used for confocal microscopy: mouse monoclonal anti- V5-tag (#R960-25, Thermo Fisher Scientific, dilution 1:500), mouse monoclonal anti-α-tubulin (#MA1-19162 ...
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bioRxiv - Pathology 2023Quote: ... The conjugation solution was prepared according to the manufacturer’s instructions with the anti-FLAG Monoclonal Antibody (FG4R) at 1:3,000 dilution (ThermoFisher Scientific, no. MA1-91878). One ml functionalized NP was centrifugated at 10,000 rpm for 5 min ...
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bioRxiv - Biochemistry 2022Quote: Recombinant hItln1 and MBL were characterized by western blotting using monoclonal mouse anti-MBL2 antibody (3E7; ThermoFisher Scientific MA1-40145; 1:50) and polyclonal rabbit anti-hItln1 antibody (Proteintech 11770-1-AP ...
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bioRxiv - Neuroscience 2023Quote: ... a series of every 6th section was immersed in 1% skim milk for 1 h at room temperature and incubated overnight at 4°C with rabbit anti-GFP monoclonal antibody (1:500, G10362, Thermo Fisher Scientific) i n PBS containing 0.1% Triton X-100 and 1% normal goat serum for 2 days at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed three times with PBS and incubated with CD15 (C3D-1) mouse anti-human monoclonal antibody (Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were incubated overnight (50 rpm, 4°C) with either mouse anti-His monoclonal (Thermo Fisher; 1:3000 in 5% skimmed milk) or anti-IFNa2 primary antibody (abcam ...
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bioRxiv - Cell Biology 2024Quote: ... Intracellular 7-ketocholesterol was measured by fixing cells in 4% paraformaldehyde and staining with 7-ketocholesterol monoclonal antibody at 1:50 (3F7, Invitrogen, MA5-27561) diluted in 1X permeabilization buffer (Invitrogen ...
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bioRxiv - Immunology 2019Quote: All the receptors used in this study were either cloned into bicistronic pEFIN3 vector (Euroscreen) for stable expression or pcDNA3 vector (Life Technologies) for transient expression (S1 Table) ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed for receptor cDNA using validated primers (S4 Table) and SYBR Green Power PCR Mix (Applied Biosystems, ThermoFisher) on an iQ5 real-time qPCR detection system (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... RT-qPCR was performed for receptor cDNA using validated primers (S4 Table) and SYBR Green Power PCR Mix (Applied Biosystems, ThermoFisher) on an iQ5 real-time qPCR detection system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2020Quote: ... or Insulin Receptor beta (ab69508) overnight at 4°C followed by anti-rabbit secondary antibody conjugated to horseradish peroxidase (ThermoFisher Scientific). Proteins were detected by Luminata Crescendo immunoblot HRP substrate (WBLUR0500Millipore Corporation ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Immunology 2020Quote: The Fab fragments for anti-SARS-CoV-2 antibodies ION_300 and ION_360 and SARS-CoV-2 receptor binding domain (RBD) were expressed in Expi293F™ cells (Thermo Fisher, A14527). The antibody:RBD complexes were mixed and co-purified by size exclusion chromatography in 20 mM Tris-HCl (pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The assay procedure is based on the manufacturer’s protocol for LanthaScreen™ TR-FRET Thyroid Receptor beta Coactivator Assay (Invitrogen, PV4686) with slight ...
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bioRxiv - Neuroscience 2020Quote: Cells were incubated with anti-human Fc receptor (0,005 µg/ml eBioscience, 14-9161-73) for 10 min in Medium A without phenol red (HBSS (Gibco, 14170-053) containing 15 mM HEPES (Lonza ...
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bioRxiv - Neuroscience 2020Quote: ... the P2X2 receptor was conditionally expressed into SST+ interneurons and selectively stimulated by laser uncaging of DMNPE-caged ATP (100μM, Life Technologies, UK). LSPS was performed using an ultraviolet (UV ...
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bioRxiv - Cancer Biology 2020Quote: ... SSOs specific to insulin receptor pre-mRNA were transfected in cells with either Lipofectamine 2000 (Catalog Number 11668030) from Life Technologies.
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bioRxiv - Cell Biology 2021Quote: The expression cassette consisting of a Kozak sequence followed by the coding sequence for human diphtheria toxin receptor (hDTR; UniProt Q53H93) was codon-usage optimized for expression in mice and synthesized (Thermo Fisher Scientific/GeneArt ...
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bioRxiv - Plant Biology 2023Quote: ... coding sequences of receptor and effector genes with or without stop codons were either synthesized as pDONR221 entry clones from GeneArt (Thermo Scientific), or were published previously (Saur et al. ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SNAP-tagged human β2-adrenoceptor or human EP2 receptors fused at the C terminus to a thermostable Nanoluciferase (Hek-ssβ2-AR-tsNluc) using Lipofectamine 3000 reagent (Invitrogen, Paisley, UK). Original SNAP tag and Nanoluciferase sequences were from NEB (Hitchen UK ...
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bioRxiv - Neuroscience 2023Quote: ... A DNA fragment containing the nucleotide sequence of the membrane-bound EGFP link via a T2A cleaving site to the TVATC66 receptor Field 69 coding sequence was designed in an inverted orientation and ordered via GeneArts (ThermoFisher Scientific). This DNA sequence was subsequently amplified by Q5 Hot Start High Fidelity DNA polymerase-based PCR (New England Bioscience ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... with 35 nM SAMMSON ASO or NTC ASO using a Multidrop Combi reagent Dispenser (Thermo Fisher Scientific). From each compound plate ...
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bioRxiv - Biophysics 2019Quote: ... 50,000 cells were seeded in 35 mm tissue culture treated plastic dishes (Nunc, ThermoFisher Scientific, Waltham, MA) and grown overnight in high glucose Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Biophysics 2019Quote: ... 50,000 cells were seeded in 35 mm tissue culture treated plastic dishes (Nunc, ThermoFisher Scientific, Waltham, MA) and grown overnight in high glucose Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Cell Biology 2022Quote: ... In case of live staining the cells were seeded in grooved 35 mm plates (Thermo Fisher Scientific) at a density of 0.04 x 106 ...
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bioRxiv - Molecular Biology 2019Quote: ... Bruns et al.[35] originally isolated the L3.6 cells which were maintained in minimum essential media (Invitrogen) supplemented with 10% fetal bovine serum ...
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bioRxiv - Genetics 2019Quote: ... 35 μg were fractionated by high-pH reversed phase chromatography using an Ultimate 3000 HPLC (Thermo Scientific) equipped with a C18 column (BioBasic-18 ...
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bioRxiv - Cell Biology 2021Quote: ... Amastigotes in murine bone marrow derived macrophages were grown on glass bottomed 35 mm dishes (Thermo Scientific) and imaged in FluoroBrite DMEM (Gibco ...
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bioRxiv - Cell Biology 2021Quote: ... and iPSC-CM for 35 minutes with tetramethylrhodamine ethyl ester (TMRE, 20 nM, Invitrogen, Cat# T-669) and Mito Tracker Green (MTG ...
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bioRxiv - Cell Biology 2022Quote: ... This agar square was removed and inverted onto a 35 mm glass bottom dish (Thermo Scientific 150682). Cells were subsequently imaged using a Zeiss Z1 inverted fluorescence microscope with a Prime 95B sCMOS camera (Teledyne Photometrics) ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions (35 μl) were then plated in complete medium consisting of DMEM medium (#10569010; Gibco-BRL) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genetics 2022Quote: ... The cell suspension was strained through a 35 μm mesh cap into a FACS tube (Gibco # 352235). Propidium iodide (1:500 ...
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bioRxiv - Developmental Biology 2019Quote: ... pax3-GR mRNA (35 pg) and zic1-GR mRNA (72 pg) synthesized with mMessage mMachine kits (Invitrogen), were co-injected into both blastomeres of two-cell stage embryos ...
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bioRxiv - Developmental Biology 2020Quote: ... and fragmented into 35-200 base pair fragments using a magnesium acetate buffer (Affymetrix, Santa Clara, CA). Ten micrograms of labeled cRNA were hybridized to Affymetrix GeneChip Mouse Gene 1.0 ST Array for 16 h at 45°C ...
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bioRxiv - Biophysics 2019Quote: ... Cells grew on the coverslip or the 35 mm cell culture dish in growth medium (DMEM [Gibco; #11880-02] containing 1x MEM NEAA [Cat#11140-035 ...
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bioRxiv - Molecular Biology 2019Quote: ... and placed in a 35 mm glass bottom dish (Thermo Scientific 150682) (Thermo Fisher Scientific, Waltham, MA). Cells were imaged using a Zeiss Z1 inverted fluorescence microscope with a Prime 95B sCMOS camera (Teledyne Photometrics ...