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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PE-conjugated rat anti-mouse GATA3 (clone TWAJ; Thermo Fisher Scientific), or FITC-conjugated rat anti-mouse Foxp3 (FJK-16s ...
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bioRxiv - Cell Biology 2023Quote: ... and goat anti-rat antibody Alexa Fluor 568 (Life Technologies, UK), both were diluted 1/1000 with 3% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... 100 µg/mL of rat tail collagen I solution (ThermoFisher Scientific) is added on the gel and incubated for 2 h at room temperature under a 50 rpm agitation ...
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Tight junction membrane proteins regulate the mechanical resistance of the apical junctional complexbioRxiv - Cell Biology 2023Quote: ... Alexa Fluor 488-conjugated donkey anti-rat IgG (Molecular Probes; #A21208), Alexa Fluor 555-conjugated donkey anti-mouse IgG (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: The following human (h) and rat (r) TaqMan assays (Thermo Fisher) were used ...
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bioRxiv - Genetics 2024Quote: ... or α-rat FITC-conjugated (for α-α-tubulin, Thermo Fisher) secondary antibodies were used.
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bioRxiv - Microbiology 2024Quote: ... and Rat-2 cells were cultured in 1x DMEM (ThermoFisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed with PBS for 3 × 10 minutes prior to incubation overnight at 4°C with the Alexa secondary cocktail: Alexa Fluor 555 dye-conjugated goat anti-rat (1:1000, Invitrogen, Eugene, OR, USA) and Alexa Fluor 488 dye-conjugated goat anti-mouse (1:600 ...
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bioRxiv - Developmental Biology 2019Quote: ... for 1 hour at room temperature and Alexa647-conjugated goat-anti rabbit or goat anti-rat secondary antibodies highly cross-adsorbed against IgG from other species (ThermoFisher Scientific, 1:200) for 1 hour at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... chips were washed with 100 ul PBS per channel and stained with the following secondary antibodies diluted 1: 100 in 5% BSA in PBS for 2 hours at room temperature: goat anti-rat Alexa Fluor 488 (Thermo Fisher Scientific A11006), donkey anti-rabbit Alexa Fluor 647 (Thermo Fisher Scientific A31573) ...
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bioRxiv - Plant Biology 2021Quote: ... Roots were incubating with the primary antibody diluted at 1/100 (anti-PI4Kα1) and with secondary antibody diluted at 1/300 (anti Rat IgG, alexa Fluor 488 conjugate, InVitrogen-Molecular Probes, #A-21210) overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The slides were washed three times for 10 min with 0.033% Triton X-100 and incubated with an anti-rat antibody conjugated with Alexa Fluor® 488 (1:1000 dilution, Thermo Fisher Scientific) in blocking buffer for 1 hr at room temperature ...
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bioRxiv - Immunology 2021Quote: ... for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088, Thermo Fisher Scientific; A18869, Thermo Fisher Scientific) for monoclonal antibodies ...
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bioRxiv - Physiology 2021Quote: ... and incubated with secondary antibody (conjugated goat anti-rat Alexa fluoro 546, 1:1,000 dilution, A11081; Invitrogen by ThermoFisher Scientific; Waltham, MA, USA) for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... Fluorescent anti-rat (Alexa 594) and biotinylated anti-rabbit coupled with streptavidin fluorescent conjugated antibody (Alexa 488) (1:1000, both Life Technologies, Carlsbad, USA).
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bioRxiv - Developmental Biology 2021Quote: ... the sections were incubated with a secondary antibody conjugated with a biotin or fluorescence label (for immunofluorescence) or biotin for immunohistochemistry (for CD31, PEG10 and CD45, 1:500 goat anti-rat IgG conjugated with Cy3 (Thermo Fisher Scientific, A10522); for Cytokeratin ...
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bioRxiv - Neuroscience 2020Quote: ... A further incubation of sections commenced in a secondary antibody solution containing 1:500 donkey anti-rat ALEXA 594 (Invitrogen, Burlington, ON, Canada) in 0.1 M TBS for 24 hours at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... sections were with the respective fluorochrome-conjugated secondary antibodies (goat anti-mouse/anti-rabbit/anti-rat, streptavidin etc, conjugated to AlexaFluor 488, 594, 633, 647, all purchased from ThermoFisher Scientific; 1:500) for 2 hours at room temperature in 5% normal goat serum and 0.5% Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2022Quote: The following secondary antibodies were used at a 1:1000 dilution for tissue and cell staining: donkey anti-rat Alexa Fluor 594 (Thermo Scientific A-21209), goat anti-rat Alexa Fluor 647 (Thermo Scientific A-21247) ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue sections were washed three times with 0.01M PBS and incubated with goat anti-rat Alexa Fluor 488 (1:1000; Invitrogen, cat no. A-11006) and donkey anti-rabbit Alexa Fluor 594 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again re-blocked with 5% NGS for 2 hr (second serum blocking) and subsequently stained with rat anti-GFAP (1:500; Invitrogen Cat. #13-0300), and rabbit anti-Megf10 (1 ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1 hr under room temperature followed by an 1-hr incubation of an alexa 488 goat anti-rat secondary antibody (Thermo Fisher, A-11006). The slides were mounted with VECTASHIELD Antifade Mounting Medium with 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... The primary antibodies used were 1:30 rat anti-N-cadherin (Developmental Studies Hybridoma Bank, DN-EX #8,) and 1:200 rabbit anti-lucifer yellow (Molecular Probes, A-5750). The secondary antibodies used were 1:500 goat anti-rabbit with Alexa fluor 633 (Molecular Probes ...
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed in 0.1M PBS and incubated in goat anti-rat Alexa Fluor 568 secondary antibody (1:500, catalog #A11077, Thermo Fisher Scientific; RRID: AB_2534121) for 1 hr ...
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bioRxiv - Microbiology 2023Quote: ... haemoglobin RNA was removed from 1 µg of RNA extracted from blood samples using the GLOBINclear-Mouse/Rat Globin mRNA Removal Kit (Thermo Fisher Scientific; AM1981) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cells were washed three times in PBS and incubated with secondary antibody (Alexa-Fluor-647 goat anti-rat, Thermo Fisher, 1:200 dilution) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated overnight at 4°C with primary antibodies diluted in blocking solution: rat anti-Tbr2 (1:500, Invitrogen 14-4875-80), rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Neuroscience 2022Quote: ... brains were fixed for 14 min in freshly prepared 4% paraformaldehyde and incubated overnight in rat anti-CD8 (1:50, Thermo Fisher #MA5-17594) and mouse nc82 (1:40 ...
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bioRxiv - Cancer Biology 2022Quote: ... The secondary antibodies were used with goat anti-Rat IgM cross-adsorbed secondary antibody DyLight 488 (Thermo fisher, SA5-10010, 1:400 dilution) or goat anti-Rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... The secondary antibodies used were goat anti-rat Alexa Fluor 488 or goat anti-mouse Alexa Fluor 596 (Thermo Fisher scientific, 1:1000). The incubation with antibodies was accomplished in a humidified chamber for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Brain slices were then incubated either 4 hours at room temperature or overnight at 4°C in 1.5% Normal Donkey Serum in PBS-T with donkey anti-rat DyLight 550 (ThermoFisher, SA5-10029, 1:1000), donkey anti-goat AlexaFluor 488 (Jackson ImmunoResearch ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged and washed 2 times in cold flow buffer and resuspended in donkey anti-rat 555 secondary antibody (ThermoFisher, A48270, 1:1000), donkey anti-guinea pig 647 secondary antibody (Biotium ...
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bioRxiv - Biophysics 2021Quote: ... AML cells were grown in suspension in twelve flasks containing 150 mL alpha minimum essential medium (Invitrogen Canada Inc., Burlington, Canada), with 5% fetal bovine serum (Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Murine dendritic cell line JAWSII was purchased from ATCC and was cultured in alpha minimum essential medium with 20% FBS (Life Technologies), ribonucleosides ...
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bioRxiv - Developmental Biology 2021Quote: Protein expression analysis by WB was performed as described (Grivas et al., 2020), using antibodies Mdka (Calinescu et al., 2009) and Alpha-Tubulin (Thermofisher scientific).
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bioRxiv - Microbiology 2019Quote: Mtb was grown as described above and processed for ChIP experiments using purified anti-PhoP (Alpha Omega Sciences) or anti-FLAG (Thermo Scientific) antibody and protein A/G agarose beads (Pierce ...
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bioRxiv - Immunology 2020Quote: ... IL-23 and tumor necrosis factor alpha (TNF-α) were quantified in lung tissue lysates and BAL using commercial ELISA kits (Invitrogen, San Diego ...
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bioRxiv - Bioengineering 2020Quote: ... We cloned the wild type Cas13a with a 3’ V5 tag (Cas13a-V5) and appended a 3’ UTR from mouse alpha-globin (Genbank accession # NM_001083955) in a pMA7 vector (GeneArt, Thermo Scientific, USA). Additionally ...
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bioRxiv - Molecular Biology 2019Quote: ... The OCI-LY19 cell line (DSMZ, ACC 528) was cultured in Minimum Essential Medium Eagle - Alpha Modification (Gibco, catalog number 12000063) supplemented with 2.2g/L of sodium bicarbonate (Thermofisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: The murine preosteoblast cell line MC3T3-E1 clone 14 was purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in alpha modified Eagle’s medium (α-MEM, Gibco, USA) supplemented with 10% FBS (HyClone ...
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bioRxiv - Immunology 2021Quote: ... Murine dendritic cell line JAWSII was purchased from ATCC and was cultured in alpha minimum essential medium with 20% FBS (Life Technologies), ribonucleosides ...
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bioRxiv - Cancer Biology 2022Quote: ... TNF alpha mouse high sensitivity ELISA kit and IL-6 mouse high sensitivity ELISA kit were from Invitrogen (Thermo Fisher Scientific), mouse CCL2/JE/MCP-1 quantikine ELISA kit ...
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bioRxiv - Physiology 2022Quote: ... Inflammatory OCLs were differentiated by seeding a total of 2×104 CD11c+ DCs/well on 24-well plates in MEM-alpha (ThermoFisher Scientific) including 5% serum (Hyclone ...
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bioRxiv - Developmental Biology 2023Quote: IVM for the GV oocytes was conducted by washing the oocytes twice and culturing in alpha-modified Eagle’s minimum essential medium (Thermo Fisher Scientific) containing 5% (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in alpha minimum essential media (αMEM; Biological Industries, 01-042-1A) supplemented with 10% fetal calf serum (FBS; Invitrogen, 10270106), 100 IU/mL penicillin ...
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bioRxiv - Neuroscience 2023Quote: ... and 62.5 ng of cDNA was amplified using TaqMan probes for vascular endothelial growth factor alpha (Vegfa) and 18S as endogenous control (Life Technologies), and SYBR green probes for cd93 ...
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bioRxiv - Bioengineering 2024Quote: ... Agarose (IB70040), alpha-Cyano-4-hydroxycinnamic acid (J67635.EXK), and activated alumina (Brockman I, basic) (6002042) were purchased from Thermo Scientific. Novozym 435 was received as a gift from Novozymes ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA, Invitrogen, respectively). Serum noradrenaline was extracted with the use of a cis-diol–specific affinity gel ...
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bioRxiv - Bioengineering 2019Quote: STP and mAb binding to the carriers was quantified indirectly using a bicinchoninic acid assay (BCA) kit (Thermo Fisher) according to the manufacture’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The resin was subsequently washed with 3 resin volumes (RV) of the same buffer before mAb P3.2 (Thermo Fisher) was eluted using 3 RV of 0.2 M glycine pH 2.5 ...