Labshake search
Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: ... HRP-anti-V5 Tag monoclonal antibody (Thermo Fisher Scientific, R96125); Anti-human GAPDH (Proteintech ...
-
bioRxiv - Molecular Biology 2023Quote: Immuno-blotting employed commercial monoclonal antibodies directed against Sprr1a (ThermoFisher), p16 (Abcam) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Mouse monoclonal anti-Ubiquitin (eBioP4D1) antibody (Invitrogen, 14-6078-82), 1:500 ...
-
bioRxiv - Cancer Biology 2024Quote: ... DKK1 was detected using DKK1 monoclonal rabbit antibody (Invitrogen, 1D12), and detected with secondary antibody conjugated to HRP (Fisher ...
-
bioRxiv - Cell Biology 2024Quote: ... and anti-CD326/EpCAM monoclonal conjugated to allophycocyanin (APC) (Invitrogen; reference # 17-5791-82 ...
-
bioRxiv - Biochemistry 2024Quote: ... The anti-RPA1 (mouse monoclonal) was purchased from Thermo Fisher scientific (Waltham ...
-
bioRxiv - Neuroscience 2024Quote: ... or a mouse monoclonal anti-CaMKIIα (6G9, Thermofisher, MA1-048). This was done as described previously ...
-
bioRxiv - Molecular Biology 2019Quote: ... was performed using the TaqMan gene expression assays (EFHA1/Micu2 rat: s235193; EFHA1/Micu2 human: s47976; Hprt1 rat: Rn01527840; Hprt1 human: Hs99999909, Applied Biosystems, Life Technologies, Carlsbad, CA), using a 7900HT Fast Real-Time System (Applied Biosystems) ...
-
bioRxiv - Biochemistry 2021Quote: ... Primary Strep-MAb classic chromeo-555 (IBA) and secondary Alexa-fluor-488 or −555 goat anti-mouse (Invitrogen) were applied sequentially with PBS washes in between ...
-
bioRxiv - Molecular Biology 2021Quote: ... Supernatant was collected 6 days later and recombinant mAb was purified using protein A agarose (Thermo Fisher Scientific) according to the manufacturer’s protocol.
-
bioRxiv - Bioengineering 2021Quote: ... cells were pre-incubated with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 µL of PBS with 2% FCS for 15 minutes at 4 °C ...
-
Dimeric prion protein ligand activates Adgrg6 but does not rescue myelinopathy of PrP-deficient micebioRxiv - Neuroscience 2020Quote: ... Briefly, sheep-anti mouse IgG paramagnetic beads (Dynal, 11201D) were coupled with anti-His mAb (Invitrogen, 37-2900) in coating buffer (PBS plus 0.1% immunoglobulin-free BSA ...
-
bioRxiv - Immunology 2021Quote: ... or with unconjugated 4.3.1.3 mouse MAb and secondary detection with goat anti-mouse Alexa-Fluor 647 (A-21235; Life Technologies). Immunofluorescence was used to boost GFP signal in TgBAC(lck:gfp)vcc6 specimens with a chicken anti-GFP primary (Abcam ...
-
bioRxiv - Microbiology 2021Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 57 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
-
bioRxiv - Microbiology 2022Quote: ... Recombinant human IgG1 mAbs were produced by co-transfection of Freestyle 293-F suspension cells (Thermo Fisher Scientific) as previously described 48 and purified by affinity chromatography using protein G sepharose 4 fast flow beads (GE Healthcare).
-
bioRxiv - Biophysics 2022Quote: ... phagosomes were washed 3X with MAB and incubated with secondary antibodies against mouse (Alexa488; A11029, Thermo Fisher Scientific) and rabbit (Alexa647 ...
-
bioRxiv - Microbiology 2020Quote: ... The c-Myc epitope was detected with mouse mAb 9E10 (59) or rabbit pAb anti-Myc (Invitrogen PA1981). His6-tagged proteins were recognized with mouse anti-His6 (R&D systems MAB050) ...
-
bioRxiv - Cancer Biology 2021Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
-
bioRxiv - Microbiology 2021Quote: ... The bound mAb was detected with adding 100 μl/well of 3,3′,5,5′-Tetramethylbenzidine (TMB) substrate (Life Technologies) and incubation at room temperature for 5 min prior to the addition of 100 μl of 3% H2SO4 to stop the reaction ...
-
bioRxiv - Microbiology 2021Quote: ... The isotyping of the selected clones was performed using the commercial Rapid ELISA Mouse mAb Isotyping kit (ThermoFisher). The kit determines the presence of the murine isotypes IgG1 ...
-
bioRxiv - Immunology 2020Quote: ... cells were pre-incubated with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience) in 100 μL of PBS with 2% FCS for 15 minutes at 4 °C ...
-
bioRxiv - Immunology 2023Quote: ... we obtained the sequence information of the 14.4.4 mAb VH and VL chain by isolating total mRNA from a 14.4.4 mAb-producing hybridoma cell line with the use of TRIzol (Invitrogen) followed by reversely transcribing the isolated mRNA into cDNA (First-strand cDNA Kit ...
-
bioRxiv - Immunology 2023Quote: ... mAbs or ACE2 protein were first biotinylated using the EZ-Link Sulfo-NHS-Biotinylation Kit (Thermo Fisher Scientific) followed by removal of unreacted biotinylation reagents using a Zeba Spin Desalting Column (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... double immunofluorescence labeling with detergent was performed on 50-µm sections with the anti-pTau mAb AT8 (ThermoFisher) plus the anti-mouse IgG H + L secondary antibody conjugated with Alexa Fluor 488 (ThermoFisher) ...
-
bioRxiv - Cancer Biology 2023Quote: ... naïve CD8+ T cells were stimulated with αCD3 and αCD28 mAb-coated dynabeads (Thermo Fisher Scientific, no. 11452D).
-
bioRxiv - Microbiology 2024Quote: ... mAb pool was dialyzed in PBS pH 7.4 using Slide-A-Lyzer G2 Dialysis Cassette 3.5K (Thermo Scientific) overnight at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... Human mAbs were expressed in ExpiCHO-S™ cells using an ExpiCHO™ Expression System Kit (Gibco, A29133). IgG were purified from cell culture supernatant 6-7 days after transfection via Protein A affinity chromatography using 2 mL or 5 mL column HiTrap MabSelect SuRe Protein A column (GE Healthcare ...
-
bioRxiv - Cancer Biology 2024Quote: ... cells were harvested with Accutase then incubated with anti-Ki67/FITC antibody (ThermoFisher, mouse mAb, clone 7B11, #MHKI6701) and stained with propidium iodide.
-
bioRxiv - Microbiology 2024Quote: ... mAbs were incubated at 37□ for 5 h with 100 µL papain immobilized on agarose resin (ThermoFisher Scientific) in PBS ...
-
bioRxiv - Immunology 2024Quote: Recombinant rabbit mAbs and Fabs were expressed in HEK293F cells using ExpiFectamine™ 293 Transfection reagent (Gibco, A14525). Human mAbs were expressed in ExpiCHO-S™ cells using an ExpiCHO™ Expression System Kit (Gibco ...
-
bioRxiv - Neuroscience 2021Quote: The supernatant from this homogenization step was used to evaluate levels of TNFα using the mouse TNF alpha High Sensitivity ELISA Kit according to manufacturer’s instructions (Invitrogen, BMS607HS). Briefly ...
-
bioRxiv - Cell Biology 2019Quote: ... The attached cells were cultured for 16 days with alpha minimum essential medium (α-MEM, Gibco BRL, Gaithersburg, MD, USA) supplemented with 20% FBS ...
-
bioRxiv - Cancer Biology 2019Quote: ... and primary fibroblasts obtained from lungs of healthy NSG or BALB/c mice 6-8 weeks of age were cultured in Minimum Essential Medium Eagle medium with alpha modification (MEMα) (ThermoFisher) supplemented with 1x MEM Non-essential Amino Acid Solution (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2019Quote: ... The NK-92 cells were obtained from DSMZ (ACC 488) and grown in in MEM Alpha medium (Gibco, #22571-020) supplemented with 5 ng/mL recombinant human interleukin 2 (Gibco ...
-
bioRxiv - Bioengineering 2021Quote: ... MSCs were thawed and allowed to recover for 24 hours in complete medium (MSC-GM) (Alpha-Minimum Essential Medium (Gibco), 10% fetal bovine serum (Hyclone) ...
-
bioRxiv - Microbiology 2020Quote: Luminex profiling was performed on whole blood that was allowed to clot for 20 minutes and then spun down using a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
-
bioRxiv - Microbiology 2022Quote: ... Luminex kits that were used are as follows a custom mouse IFN kit (IFN alpha, IFN beta, IL-28, Invitrogen), mouse cytokine 23-plex (Bio-Rad ...
-
bioRxiv - Cell Biology 2022Quote: ... Primary bone marrow monocytes from isolated from the tibiae by flushing the marrow cavity with osteoclast media (phenol red-free alpha-MEM (Gibco) with 25 units/mL penicillin/streptomycin (Invitrogen) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Bone marrow was collected from the femoral cavity of 7-day-old SD rats by flushing of medium using a 22-gauge needle and cultured in complete growth medium (alpha-modified eagle’s medium (αMEM, GIBCO, USA), 10% fetal bovine serum (Sigma ...
-
bioRxiv - Cancer Biology 2019Quote: ... MSCs from bone marrow Ewing patients were isolated by density-gradient centrifugation using Ficoll technique and were cultured in alpha MEM (Gibco), MSC-qualified serum (Gibco) ...
-
bioRxiv - Immunology 2019Quote: ... Cytokine measurement on tissue homogenate was assessed using ELISA kits according to the manufacturer instructions (Mouse IFN gamma Uncoated ELISA, Invitrogen, #88-7314; Mouse TNF alpha Uncoated ELISA, Invitrogen, #88-7324 ...
-
bioRxiv - Cell Biology 2019Quote: ... BM was flushed from cut femurs and tibiae using 3-ml syringes with Minimum Essential Medium Alpha (αMEM without ribonucleosides or deoxyribonucleosides; Gibco, Life Technologies cat ...
-
bioRxiv - Cell Biology 2021Quote: ... Cellmatrix Type I-C) fibril-coated plate (defined as passage 0, population doubling level 0) and cultured in expansion medium (alpha-MEM (Invitrogen), 10% FBS (Hyclone) ...
-
bioRxiv - Cell Biology 2022Quote: Human breast cancer (MCF-7) cells were obtained from ATCC and cultured every two days in Minimum Essential Medium (MEM) Alpha media (Gibco) supplemented with 1 mM sodium pyruvate ...
-
bioRxiv - Developmental Biology 2023Quote: ... the cell culture medium was changed into myogenic differentiation medium composed of alpha-MEM (Nacalai tesque) with 5% of KSR (GIBCO) and 500ng/ml of Dox ...
-
bioRxiv - Cell Biology 2023Quote: Alexa 647 labelled Goat anti-Rabbit IgG secondary antibody and anti-alpha tubulin primary antibody was purchased from Thermo Fisher Scientific (Australia) ...
-
bioRxiv - Cell Biology 2023Quote: Pooled HUVEC sample sets were grown to confluence before treatment with or without recombinant tumour necrosis factor alpha (TNF; 10 ng/mL) (ThermoFisher) in cell culture medium ...
-
bioRxiv - Molecular Biology 2023Quote: ... Chemical composition of the sample surfaces was analyzed by X-ray photoelectron spectroscopy (XPS, Thermo Fisher Scientific K-Alpha XPS) with a 400 μm microfocused monochormatic Al Kα X-ray source ...
-
bioRxiv - Developmental Biology 2024Quote: Primary cells isolated from bone marrow were cultured on a OP9-DL1 monolayer stromal cells(Holmes and Zuniga-Pflucker, 2009) at 37℃ in 5% CO2 conditions with standard culture medium [80% MEM-alpha (Gibco), 20% Fetal Bovine Serum (Corning) ...
-
TAK1 operates at the primary cilium in non-canonical TGFB/BMP signaling to control heart developmentbioRxiv - Developmental Biology 2024Quote: ... The P19CL6 wildtype and Tak1 and Tab2 mutants were cultured in T25 flask at 37°C and 5% CO2 in MEM alpha medium (Gibco) supplemented with 10% FBS (Gibco ...