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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for Nicotinic Acetylcholine Receptor alpha 1 mAb 35 Rat Monoclonal since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were incubated with the following primary antibodies in blocking buffer overnight at 4 °C: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific) and mouse anti-OCT4 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... slides were incubated overnight at 4 °C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibodies include: goat-anti-rat polyclonal Alexa Fluor and goat-anti-mouse polyclonal Alexa Fluor 488 (both 1:1000, Thermofisher, Waltham, MA).
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bioRxiv - Cancer Biology 2019Quote: ... Slides were then washed with PBS and incubated with Alexa Fluor 488-conjugated chicken anti-rat antibody at 1:200 dilutions (Life technologies, A21470). Slides were washed wit PBS and incubated for 45 minutes at room temperature with mouse anti-BrdU (Becton Dickinson ...
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bioRxiv - Immunology 2019Quote: ... lung tissues were incubated with primary antibodies diluted in PBS with 0.3% TritonX-100 on a rocker at 4° C overnight. Primary antibodies used (Fig. 5) were: rat anti-E-cadherin (E-Cad, 131900, Invitrogen; 1:1000), goat anti-CCSP (1:2,500 ...
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bioRxiv - Genetics 2019Quote: Coverslips were incubated for one hour at 37°C in 1mL of of 1:30 solution of Collagen I Rat Protein (Thermo Fisher Scientific) to 1X PBS ...
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bioRxiv - Neuroscience 2021Quote: Bacterial expression constructs coding for His6/SUMO-tagged fusion proteins of rat Shank3 (residues 1-348, SPN-Ank) were generated in pET-SUMO (Thermo Fisher Scientific) as described before (17) ...
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bioRxiv - Plant Biology 2021Quote: ... Roots were incubating with the primary antibody diluted at 1/100 (anti-PI4Kα1) and with secondary antibody diluted at 1/300 (anti Rat IgG, alexa Fluor 488 conjugate, InVitrogen-Molecular Probes, #A-21210) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were washed three times in PBS and stained with secondary antibodies (Goat anti-Rabbit Alexa Fluor 488, Goat anti-Rat Alexa Fluor 647, and/or Goat anti-Mouse Alexa Fluor 647; Invitrogen, 1:1000) and DAPI (1:10,000 ...
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bioRxiv - Bioengineering 2021Quote: ... Collagen-coated surfaces were used as a native protein control and were prepared by adding 0.15 mL/cm2 of 50 μg/mL type 1 rat-tail collagen (Thermo Fisher Scientific™) in 0.02 N acetic acid (Thermo Fisher Scientific™) ...
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bioRxiv - Physiology 2021Quote: ... Membrane damage was assessed using anti-IgG staining using an anti-rat antibody (IgG H+L AlexaFluor® 647 1:100, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... on ice for 1 hour and followed by incubation with sheep anti-rat IgG Dynabeads M-450 (Invitrogen, Cat#11035, 50 μl) for 30 mins ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for one hour with the chicken anti-rat AlexaFluor 647 secondary antibody (1:400, #A-21472, RRID: AB_2535875, Thermo Fisher Scientific, USA). For PV staining ...
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bioRxiv - Microbiology 2020Quote: ... followed by three 5 min washes in PBS-T and a subsequent incubation with species-specific secondary antibody (goat-anti-rabbit/rat/mouse IgG-HRP, Invitrogen 1:2500) for 1 h in 5 % milk in PBS-T ...
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bioRxiv - Immunology 2020Quote: ... and then incubated with secondary antibodies for 30 min at room temperature (AF555-goat anti-rat IgG (Invitrogen, Carlsbad CA, 1:500); AF647-sAv (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: The concentration of secreted Tgfβ1 ligand levels was measured by taking 3-day conditioned media at d5 of culture with Tgf beta-1/LAP Rat Uncoated ELISA Kit with Plates by following the manufacturer’s protocol (ThermoFisher Scientific, Waltham, MA). The cells were cultured in a 2% FBS media for ELISA ...
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bioRxiv - Physiology 2020Quote: ... Sections were then incubated with Alexa Fluor® 488 conjugated goat anti-rat immunoglobulin G (IgG; [H+L]; 1:100, Life Technologies) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... fixed cells were rinsed in PBS and incubated 1 hour at RT 10% blocking solution in PBS with a mixture of Alexa Fluor 488 conjugated goat anti-rat IgG antibody (diluted 1:300, Cat. A-11006, Molecular Probes Inc.) and Alexa Fluor 555 conjugated goat anti-rabbit IgG antibody (diluted 1:300 ...
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bioRxiv - Developmental Biology 2021Quote: ... cells were washed three times in washing solution for 5 min at RT and incubated with secondary antibodies (donkey anti-rat Alexa Fluor 488 (1:200, A-21208, Thermo Fisher Scientific) and donkey anti-mouse Alexa Fluor 555 (1:200 ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then stained with the following antibodies: rat CD31-eFluor450 (1/500; eBiosciences™, Thermo-Fisher Scientific, San Diego, CA, USA), rat CD45-eFluor450 (1/500 ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 488 donkey anti-mouse IgG and Alexa Fluor 647 donkey-rat IgG (1:1,000; Thermo Fisher Scientific, Rockford, IL, USA). Liver ...
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bioRxiv - Developmental Biology 2022Quote: ... The samples were then washed in PBST and incubated with Alexa Fluor 555 donkey anti-rat IgG secondary antibody (1:500; Thermo Fisher Scientific) for 1 h at 28°C ...
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bioRxiv - Genetics 2022Quote: ... The following secondary antibodies were used at 1:200: Alexa Fluor 555 goat anti-rat (Thermo Fisher, cat# A-21434, RRID: AB_2535855), FITC goat anti-mouse (Jackson Immunoresearch Laboratories ...
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bioRxiv - Immunology 2022Quote: ... The leaflets were then incubated for 2 days in the dark at 4°C with goat anti-Rat IgG Cross-adsorbed Alexafluor 594 secondary antibody dilution 1:500 (#A11007, Thermo Fisher Scientific), washed 3 times in 1 mL PBS (pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies (Donkey anti-goat alexa fluor 594, Donkey anti-rabbit alexa fluor 594, Donkey antirabbit alexa fluor 488, Donkey anti-rat alexa fluor 594; Invitrogen, 1:1000) were incubated with PBT-BSA for 1h at room temperature and 24h at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... slides were washed with PBS and incubated with goat anti-rat antibody labelled with Alexa Fluor™ 647 (1:500, ThermoFisher Scientific). Thereafter ...
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bioRxiv - Neuroscience 2022Quote: ... anti-rabbit, and anti-rat antibodies conjugated with Alexafluor dyes (Alexa 546, and Alexa 633) were used as secondary antibodies (ThermoFisher, 1:500). Immunolabeled sections were mounted on glass slides in PBS buffer with 70% glycerol for confocal LSM scanning ...
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bioRxiv - Biochemistry 2024Quote: ... The next day cells were washed 3x with PBS and probed with secondary antibodies: 1:2000 Alexa Fluor® 488 Goat Anti-Rat IgG (Invitrogen, 10729174) or 1:1000 phalloidin (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Slides were washed in PBS-Tween (0.5% Tween 20 in 1x PBS) and incubated in goat anti-rat IgG Alexa Fluor® 488 (1:200; A11006; Life Technologies) antibody for 45 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... for 48 hours at 4°C and then secondary antibodies donkey anti-rat Alexa Fluor 488 and donkey anti-goat Alexa Fluor 555 (1:300, Invitrogen, Waltham, MA) at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes and microglia/macrophage cells were labeled using rat anti-GFAP (1:300; Cat# 13-0300, Thermo Fisher Scientific, Waltham, MA, U.S.A) and rabbit anti-IBA1 (1:500 ...
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bioRxiv - Developmental Biology 2023Quote: ... and the following secondary antibodies diluted in blocking buffer: donkey anti-rat Alexa Fluor 488 (1:400, A-21208, Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 555 (1:400 ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies diluted in blocking buffer: rat anti-SOX2 (1:200, 14-9811-82, Thermo Fisher Scientific), goat anti-T (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% w/v Triton X-100 and incubated with goat anti-rat Alexa Fluor 488 (1:1000, catalog no. A11029, Thermo Fisher Scientific) secondary antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... 1% w/v Triton X-100 for 5 min and then incubated for 1 hour at room temperature with the following secondary antibodies: goat anti-rat Alexa Fluor 488 (1:1000, catalog no. A11006, Thermo Fisher Scientific), goat anti-chicken Alexa Fluor 488 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... used in combination with a 1:500 secondary Alexa Fluor 647 donkey anti-rat antibody (Invitrogen Thermo Fisher Scientific, cat. #A-21209).
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then washed twice into RPMI medium and seeded in a rat tail collagen-1 (5μg/cm2; Gibco, Carlsbad, CA, USA) coated flask for cell culture ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Microbiology 2023Quote: ... 1:2,000 rat α-LAMP1 (Developmental Studies Hybridoma Bank) primary antibodies and 1:500 Alexa 488-conjugated goat α-rabbit and Alexa594-conjugated goat α-rat secondary antibodies (ThermoFisher) in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissue sections were washed twice with PBS and incubated with secondary antibodies (goat-anti-rat-A568, cat# A11077, Invitrogen, 1:250) and (goat-anti-rabbit-A568 ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary antibodies at 1:500 dilution in either 10% NGS in PBS (goat anti-rat, Alexa Fluor 647, Thermo Fisher, A21247) or 1% BSA in PBS (donkey anti-rabbit ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation with the anti-rat and anti-rabbit IgG secondary antibody conjugated to Alexa Fluor 488 and 546 (1:400, Thermo Fisher Scientific) overnight at 4°C ...
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bioRxiv - Bioengineering 2023Quote: ... the embryos were stained with secondary antibodies with Alexa 488 goat anti-rat IgG and Alexa 594 goat-anti-mouse IgG (1:250, Invitrogen, Carlsbad, CA) in blocking buffer overnight ...
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bioRxiv - Bioengineering 2023Quote: ... sections were incubated with corresponding Alexa Fluor-conjugated secondary antibodies (Donkey Anti rat-AlexaFluor 488, Donkey Anti rabbit-AlexaFluor 647, Donkey Anti goat-AlexaFluor 568, dilution 1:200, Thermo Fisher Scientific). Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Immunology 2024Quote: ... Whole- mounts were then washed 3 times for 5 min at room temperature in PBS followed by incubation with Alexa Fluor 568 anti-rat (Invitrogen, 1:500) for 1 hour at room temperature in PBS with 1% BSA and 0.5% Triton-X-100 ...
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bioRxiv - Cell Biology 2024Quote: ... abdominal carcasses were incubated in AlexaFluor conjugated secondary antibodies (goat anti-mouse 488, goat anti-mouse 568, or goat anti-rat 488, ThermoFisher; 1:250) for two hours at room temperature and protected from light.
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bioRxiv - Biochemistry 2024Quote: ... The following secondary antibodies were used at a 1:1000 dilution for cultured cells: donkey anti-rat Alexa Fluor 488 (Invitrogen A-21208) and donkey anti-mouse Alexa Fluor 488 (Invitrogen A-21202) ...
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bioRxiv - Cell Biology 2024Quote: ... and rat pAbs horseradish peroxidase-conjugated (HRP-Ab) (#111-035-003, #115-035-003, Jackson Immunoresearch Laboratories; #31470, Invitrogen; 1:5000 dilution).
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bioRxiv - Neuroscience 2024Quote: ... Sections were blocked with 1% normal goat serum (NGS) in 1X PBS for 1 h followed by incubation with primary antibodies [rat anti-mCherry (Thermo Fisher, #M11217)] overnight at 4°C for standard immunohistochemistry.
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bioRxiv - Neuroscience 2021Quote: ... and β-actin (Mouse Monoclonal anti-β-actin antibody Ambion, Thermo Fisher Scientific ...