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Citations for Thermo Fisher :
3351 - 3400 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... diluted in blocking buffer: Alexa Fluor 488 donkey anti-mouse IgG (H+L) (1:500, Invitrogen A-21202), Alexa Fluor 488 donkey anti-goat IgG (H+L ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 46-48 h incubation in primary antibodies for CB (1:50, Thermo Fisher, USA, RRID: AB_2068509) and PV (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... A total of 50 ng total RNA was transcribed using Maxima H Minus Reverse Transcriptase (Thermo Fisher Scientific). Full length transcripts were selected by PCR amplification using the LongAmp Taq Master Mix and the product was purified with AMPure XP beads (Beckman Coulter) ...
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bioRxiv - Immunology 2023Quote: ... PVDF membranes were incubated with anti-puromycin Ab overnight at 4°C and secondary Ab RRID:AB_330924 at room temperature for 2 h and revealed by SuperSignal West Dura and iBright imaging system (ThermoFisher). After stripping ...
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bioRxiv - Microbiology 2023Quote: ... The cell supernatants were discarded at 5 h after seeding and replaced with Neurobasal plus Medium (Gibco, A3582901) containing 2% B-27 Plus (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... and Alexa Fluor™ 568 Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody (ThermoFisher # A-11011).
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bioRxiv - Cell Biology 2023Quote: ... followed by a 2 h incubation at 45 °C with proteinase K (final concentration 0.24 μg/μL, Ambion). Samples were de-crosslinked overnight at 65 °C by adding NaCl to a final concentration of 200 mM ...
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bioRxiv - Cell Biology 2023Quote: ... For immunofluorescence secondary antibodies used were Alexa fluor 488 goat anti-rabbit IgG (H+L) (A11034, Life Technologies) and Cy3 goat anti-rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-mouse IgG (H+L) cross-adsorbed Alexa Fluor 405 goat polyclonal (Invitrogen, #A-31553, 1:250).
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used were donkey anti-mouse IgG (H+L) Alexa Fluor 488 (Invitrogen; A32766; IF, 1:500), donkey anti-rabbit IgG (H+L ...
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bioRxiv - Biophysics 2024Quote: ... and goat anti-mouse IgG (H + L) cross-adsorbed secondary antibody (Alexa Fluor™ 555) (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... the secondary antibodies Alexa Fluor 594 Goat Anti-rabbit immunoglobulin G (IgG) (H/L) antibody (1:1,000 (Invitrogen)) or Alexa Fluor 488 Goat Anti-rabbit IgG (H/L ...
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bioRxiv - Cancer Biology 2024Quote: ... The secondary antibodies Alexa Fluor 594 Goat Anti-rabbit immunoglobulin G (IgG) (H/L) antibody (1:1,000 (Invitrogen)) and Alexa Fluor 488 Goat Anti-mouse IgG (H/L ...
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bioRxiv - Microbiology 2024Quote: ... mAbs were incubated at 37□ for 5 h with 100 µL papain immobilized on agarose resin (ThermoFisher Scientific) in PBS ...
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bioRxiv - Immunology 2024Quote: ... H chain and L chain fragments were cloned into pCDNA 3.4 Abs expression vectors (Thermo Fisher Scientific Inc) in frame with either human IgG (Igk ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor Plus 488 Goat anti-Rat IgG (H+L) (for SST stain; Catalog # A48262, Invitrogen; RRID: AB_2896330), and (2 ...
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bioRxiv - Developmental Biology 2024Quote: ... The secondary antibodies were Alexa Fluor 488 donkey anti-Rat IgG (H+L) (Thermo Fisher, A21208; 1:500) and Alexa Fluor 555 goat anti-Mouse IgG (H+L ...
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bioRxiv - Physiology 2024Quote: ... and labeled by isobaric tags for 1.5 h (TMT 10-plex or 16-plex kit, Thermo Scientific, USA). The reaction was quenched (0.3% hydroxylamine ...
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bioRxiv - Genomics 2024Quote: ... using a prepared mix of Maxima H Minus RT Buffer and Reverse Transcriptase 200U/µl (Cat#: EP0752, ThermoFisher), RiboLock RNAse inhibitor 40U/µl (Cat# ...
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bioRxiv - Evolutionary Biology 2024Quote: ... FM1-43 membrane stain (1 μM, exc. 488 nm em. 580–620 nm, Invitrogen, incubation time: 24 h), and membrane impermeable Calcein (0.7 mg/10 mL ...
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bioRxiv - Genomics 2024Quote: ... media was removed at 24 h after plating and replaced with media including 200 nM MitoTracker CMSROX (Gibco) and either 2 µg/ml Dox or no Dox ...
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bioRxiv - Bioengineering 2024Quote: ... and Alexa Fluor 594 Donkey anti-Rabbit IgG (H+L) secondary antibody (4 μg/ml; A21207; ThermoFisher Scientific) in PBS solution with 1.5 % BSA for 60 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed and then incubated with corresponding IgG (H+L) conjugated with Alexa Fluor 568 (Invitrogen, A11004) or Alexa Fluor 488 (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... The corresponding cDNA was synthesized with Revert Aid H Minus First Strand cDNA synthesis kit (Thermo Scientific, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... and Alexa Fluor 488 or Alexa Fluor 647 donkey anti goat IgG (H+L) (Invitrogen, #A32814, A-21447). The samples were fixed with 2% PFA before running through BD LSRII Green (BD bioscience) ...
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bioRxiv - Microbiology 2024Quote: ... Secondary antibodies used: Goat Anti-Rabbit IgG Highly Cross-Adsorbed (H+L) Alexa Fluor 568 (Invitrogen; #A-11036), Goat Anti-Mouse IgG Highly Cross-Adsorbed (H+L ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was removed and donkey anti-Mouse IgG (H+L) Alexa Fluor™ 488 (#A21202, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and Horseradish peroxidase (HPR)-conjugated goat anti-mouse IgG (H+L) secondary antibody were supplied by Thermo Fisher Scientific Inc ...
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bioRxiv - Cancer Biology 2024Quote: ... Next Oligo-dT primed reverse transcription (RT) was performed using Maxima H Minus reverse transcriptase (Life Technologies, EP0753) and a template-switching oligonucleotide (TSO ...
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bioRxiv - Molecular Biology 2024Quote: ... After 48 (HEK293T ACE2) or 72 h (A549 ACE2) total RNA was extracted using TRIzol (Thermo Fisher, 15596018) as per manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by a 1.5 h protein-chromatin pull-down with 20 µl protein G Dynabeads (Thermo Fisher, 10003D). ChIP samples were washed extensively ...
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bioRxiv - Microbiology 2024Quote: ... and Alexa fluor 488-conjugated Donkey anti-Sheep IgG (H+L) (Thermo Fisher Scientific, Cat. no. A-11015) secondary antibodies ...
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bioRxiv - Biophysics 2024Quote: ... according to source species selectivity: Goat anti-mouse IgG (H+L) HRP-conjugated secondary antibody (Thermo Fisher, USA) and goat anti-rabbit HRP-linked IgG secondary antibody (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... 24 h post-transfection the GFP-expressing cells were enriched under 1mg.mL-1 of geneticin (G418 sulfate; Gibco) selection for 10 consecutive days ...
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bioRxiv - Synthetic Biology 2024Quote: ... and separated by electrophoresis at 120 V for 2 h using nuPAGE MES SDS running buffer (Invitrogen, NP0002). The gels were transferred onto 0.2 µm PVDF membranes (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... goat anti-rabbit IgG (H+L) Highly Cross-Absorbed Secondary Antibody Alexa Flour 594 (1:200) (ThermoFisher, A32740) and Goat anti-Mouse IgG (H+L ...
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bioRxiv - Immunology 2024Quote: ... and the culture supernatant was collected after 16 h with an IFN human Uncoated ELISA Assay Kit (Invitrogen).
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bioRxiv - Synthetic Biology 2024Quote: ... cells were incubated with secondary antibody Alexa Fluor 488 goat anti-rabbit IgG (H + L) (ThermoFisher; Cat# A11034) for 45 min in the dark at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... for 1 h at room temperature and incubated with primary antibodies (1:1000 dilution for anti-Calnexin [ThermoFisher, PA5-19169] ...
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bioRxiv - Cell Biology 2024Quote: ... secondary antibodies (1:500 dilution) were applied: donkey anti-mouse IgG (H+L) DyLight 488 (Invitrogen; SA5-10166) and goat anti-rabbit IgG (H+L ...
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bioRxiv - Immunology 2024Quote: ... Proteins were digested for 18 h at 25 °C with trypsin/LysC (Thermo Fisher Scientific, Rockford, IL, USA). Peptides were separated on an Evosep one (Evosep ...
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bioRxiv - Immunology 2024Quote: ... DyLight 800 4× PEG-conjugated goat anti-mouse IgG(H+L) (catalog number SA5-35521; Thermo Fisher Scientific) (1:10,000) ...
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bioRxiv - Immunology 2024Quote: ... and Alexa Fluor 633-conjugated donkey anti-goat IgG(H+L) (catalog number A-21082; Thermo Fisher Scientific) (1:1,000).
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bioRxiv - Molecular Biology 2024Quote: ... Second antibodies used were the Alexa Fluor 488 donkey anti-Mouse IgG (H+L) (Life technologies, A21203,1:2000) and Alexa Fluor 488 donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Genomics 2024Quote: ... cDNA was treated with 1 μL each of RNase A and RNase H (NEB M0297S and ThermoFisher, EN0531) for 30 min at 37°C ...
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bioRxiv - Zoology 2024Quote: ... KG) for 1 h at room temperature and then mounted in ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific) between two cover slips.
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with PBS and incubated for 4 h in Earlés balanced salt solution (EBSS; Gibco #24010043).
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bioRxiv - Biochemistry 2024Quote: ... equipped with the heated electrospray ionization (H-ESI) source and coupled to a Vanquish UPLC system (Thermo Fisher). A reversed-phase column (CORTECS UPLC T3 ...
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bioRxiv - Cancer Biology 2024Quote: ... and/or Alexa Fluor 594 chicken anti-rabbit IgG (H+L) (Invitrogen, Cat No. A21442, 1:2000 dilution) secondary antibodies for 30 min at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... 24 h later the media was replaced with mesodermal induction media composed by DMEM/F12 (31331-028, Gibco) and Neurobasal medium (21103-049 ...