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Citations for Thermo Fisher :
3501 - 3550 of 4593 citations for 6 Nitrochrysene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... cells were plated in 24-wells 24 hours prior to lentivirus exposure and RNA lysates were collected 6 days later using TRIzol® (Invitrogen). For Western blotting ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were rinsed 6 times in PBS and left washing in PBS until mounted and coverslipped in ProLong Diamond Antifade Mountant (ThermoFisher #P36961) and then left to dry in the chemical cabinet overnight at RT in the dark ...
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bioRxiv - Cell Biology 2021Quote: ... and 100 μg of the total proteins from each cell line were labelled with 6-plex isobaric tandem mass tag (TMT6) reagents (Thermo Scientific) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... approximately 450,000 mycoplasma-free cells were seeded into two wells of a 6-well plate in DMEM (11995-065; Thermo Fisher Scientific) containing 10% FBS (PS-FB3 ...
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bioRxiv - Cell Biology 2021Quote: ... 5×104 cells/cm2 were seeded on 6-well plates (Cell Coat, UK) and cultured in RPMI medium (Mediatech, Los Altos, CA, USA) containing GlutaMAX (Life Technologies), penicillin/streptomycin ...
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bioRxiv - Neuroscience 2020Quote: ... Bone marrow was flushed from femurs and tibiae and bone marrow progenitor cells were cultured for 6 days on Petri dishes (Thermo Scientific) in Mφ medium [(DMEM high glucose ...
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bioRxiv - Cell Biology 2021Quote: ... with 0.4 µm pore size in 6 well plates with 1 ml Slice Culture Medium as follows: 50% MEM with GlutaMAX (Gibco, 41090036) 18% EBSS ...
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bioRxiv - Cell Biology 2021Quote: Melanoma cells were seeded at a density of 6×105 cells/ml in 12-well plates and lysed on the next day with LDS buffer 1X (Life Technologies). Lysates were denatured at 95°C for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... One microgram of siRNA was transfected in one well of a 6-well using Lipofectamine RNAiMAX transfection reagent according to the manufacturer protocol (Thermofisher Scientific). Cells were treated with siRNA for 24h.
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bioRxiv - Plant Biology 2021Quote: ... Tissues were counterstained with DAPI (4′,6-diamidino-2-phenylindole, 1 μg/ml in PBS) and mounted with ProLong Gold (ThermoFisher Scientific) using #1.5 coverslips (thickness ...
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bioRxiv - Microbiology 2020Quote: ... HAP1 cells with a low passage number were seeded into 6 well microtiter plates at a density of 5 × 105 cells/well in IMDM medium (#21980032, ThermoFisher Scientific) containing 10 % FBS (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... just-subconfluent T150 flasks were transfected with 8.75 μg of HIV-1 YU2 or HIV-1 YU2 lacking Vpr (HIV-1 YU2 ΔVpr) and 30 μl Fugene 6 in 500 μl Optimem (Thermofisher Scientific). To make VSV-G pseudotyped HIV-1 GFP ...
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bioRxiv - Microbiology 2020Quote: ... were used to amplify viral RNA and 18S rRNA primers were used to amplify cellular RNA using the QuantStudio 6 Flex RT-PCR system (Applied Biosystems). Relative quantities of viral and cellular RNA were calculated using the standard curve method (112) ...
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bioRxiv - Microbiology 2020Quote: ... Light organs were then counterstained overnight with a 1:750 dilution of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) in 5x SSC-Tween before mounting on slides with Vectashield (Vector Laboratories ...
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bioRxiv - Neuroscience 2021Quote: Hair cell nuclei were specifically labeled by briefly exposing free-swimming larvae to 4’,6-Diamidino-2-Phenylindole (DAPI; Invitrogen/ThermoFisher) diluted to 2.5 μg/ml in E3 ...
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bioRxiv - Plant Biology 2021Quote: ... Quantitative RT-PCR was performed using ChamQ Universal SYBR qPCR Master Mix (Vazyme) and a QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems). Transcript abundances were calculated using the delta-Ct method47 and normalized to the transcript levels of ACTIN2 (AT3G18780) ...
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bioRxiv - Plant Biology 2021Quote: ... approximately 0.2 grams of leaf petioles were homogenized using a HOMEX 6 homogenizer (Bioreba AG, Reinach, Switzerland) and TNA extracts were prepared using a MagMAX-96 viral RNA isolation kit (ThermoFisher Scientific) per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted TNA samples were analyzed by reverse transcription quantitative PCR (RT-qPCR) using TaqMan probes on the QuantStudio 6 Flex Real-Time PCR System (ThermoFisher Scientific) as described previously (Osman et al. ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with MVA or MVAΔE5R at MOI 10 for 6 h in the presence of MG132 (25 μg/ml) and lysed in RIPA lysis buffer (ThermoFisher, 89901) on ice for 30 min ...
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bioRxiv - Genetics 2021Quote: ... cells were washed and suspended in flow cytometry buffer containing 1µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... total RNA from infected (MOI 0.01) Vero E6 cells (1.2 x 106 cells/well, 6-well format, triplicates) was extracted using TRIzol reagent (Thermo Fisher Scientific), and used in RT-PCR reactions to amplify a fragment of 1,174 bp around the RBD of the S gene ...
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bioRxiv - Neuroscience 2020Quote: ... Surviving cells were harvested on Day 2 and plated on either matrigel coated 24 well plates at a concentration of 100,000 cells/well for ICC studies or 1×106 cells/well of a 6 well plate for drug exposure studies in neural differentiation media consisting of Neurobasal media (Thermo Fisher), B27 (Thermo Fisher) ...
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bioRxiv - Neuroscience 2020Quote: ... The medium was changed every day and cells were passaged every 5-6 days using PBS without Calcium and Magnesium (Life Technologies).
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bioRxiv - Microbiology 2020Quote: ... and the bacterial pellets resuspended in PBS and supplemented with a 1:1 ratio of 2X LIVE/DEAD solution (6 μM SYTO-9 stain and 30 μM propidium iodide) from LIVE/DEAD® BacLight kit (Invitrogen). After incubation in the dark for 15 min ...
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bioRxiv - Molecular Biology 2020Quote: ... K562#17 cells were transfected with 32 μg of barcoded IPR-PB-BC plasmid library and 6 μg of PB transposase plasmid using Lipofectamine 2000 (ThermoFisher #11668019). Mock-transfected (without PB transposase ...
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bioRxiv - Plant Biology 2020Quote: ... Sections were cut at 4-6 μm and dried from a drop of dH2O onto Probe On Plus slides (ThermoFisher Scientific). Tissues sections were stained with 0.01% aqueous Toluidine Blue O ...
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bioRxiv - Molecular Biology 2020Quote: ... each ribose-seq library was loaded on a 6% non-denaturing polyacrylamide gel and stained using 1X SYBR Gold (Life Technologies) for 40 min.
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bioRxiv - Molecular Biology 2020Quote: ... once with 1X SSC at room temperature for 15 min and twice with 0.5X SSC at room temperature for 15 min and finally with 1X PBS buffer containing 0.5 µg/mL 4′,6-diamidino-2-phenylindole (DAPI) and mounted onto ProLong Gold antifade reagent (Thermo Fisher Scientific) mounting media.
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bioRxiv - Cell Biology 2020Quote: ... with 106 viable cells/well in a 6-well plate precoated with matrigel in Williams E medium (Thermo Fisher # 12551-032) + 1x ITS (Thermo Fisher # 41400045 ...
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bioRxiv - Cell Biology 2020Quote: ... and Ctns-/- (n=6) mice were euthanized and the kidneys immediately removed and stored at −80°C in RNA Later (Life Technologies). Tissues were subsequently ground using Precellys 24 (Bertin Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Cas9-expressing hCMEC/D3 cells were each transduced separately by lentiviral particles expressing sgRNAs and selected by puromycin resistance at 10 μg/mL for 6 days (Life technologies). sgRNA sequences are listed in Table 2.
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bioRxiv - Cell Biology 2021Quote: ... were transiently transfected into 8 × 104 U2OS 2-6-3 cells in 4-well chamber slides using Lipofectamine 2000 reagent (Invitrogen, 11668019) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Embedded tissues were cut into 3-6 µm sections using a paraffin microtome (Leitz) and placed on SuperFrost® Plus slides (Menzel Gläser, Thermo Scientific). Deparaffination and antigen retrieval was done as described previously (Link et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 300 pmol of each siRNA and 6 μg of each DNA was electroporated within the dedicated tip (Thermo Fisher Scientific, MPK10096). Electroporation parameters consisted in 4 pulses of 10 ms width and a voltage of 1230 V ...
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bioRxiv - Biochemistry 2022Quote: ... 5 μl of each binding reaction was loaded directly onto a pre-run 6% acrylamide DNA retardation gel (Thermo Fisher Scientific) and electrophoresed at ≤20 V cm−1 at 4 °C ...
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bioRxiv - Biochemistry 2022Quote: Exosomes from mice plasma were harvested from age matched WT and SR-/- mice (6-8 weeks) plasma using the total exosome precipitation reagent for plasma (Invitrogen #4484451) as per instructions ...
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bioRxiv - Molecular Biology 2022Quote: βTC-6 cells (43) (ATCC, Manassas, VA) were cultured in low glucose DMEM (Dubelco’s Modified Eagle’s Medium) (Fisher Scientific, Waltham, MA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2022Quote: ... Transfection of 2 µg pSpCas9-BB-2A-GFP-sgYBX1 was carried out with 6 µl of Lipofectamine 2000 (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... spheroids were transferred to an ultra-low attachment 6-well plate placed on an orbital shaker at 65 rpm and medium was changed to Neurobasal medium (Gibco ThermoFisher) supplemented with 2% B27 supplements (Gibco ThermoFisher) ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR was performed in three technical replicates for each sample using QuantStudio 6 Flex Real-Time PCR sequence detection system (Applied Biosystems) and FastStart Universal SYBR green Master mix (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
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bioRxiv - Genomics 2022Quote: ... 2.6 μL nuclease-free water and 5 μL cDNA template on the ABI Prism 7500 Fast Sequence Detection System (Life Technologies). Thermo-cycling conditions were as follows ...
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bioRxiv - Microbiology 2022Quote: ... from an overnight culture were washed with PBS and labeled with 8mg/ml sulfosuccinimidyl-6-[biotin-amido] hexanoate (Sulfo-NHS-LC-LC-Biotin, 21338, ThermoFisher Scientific) for 30 minutes at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... 5 mg BA disks were prepared by transferring 10 μL of preheated 500 mg/mL BA in Dimethyl sulfoxide (DMSO) stock to blank antimicrobial disks (6 mm, Fisher Scientific). Single disks containing either 5 mg BA or 25 μg FLC (6 mm ...
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bioRxiv - Genetics 2022Quote: ... Cells (1-2.5 x106/mL) were then cultured at 37 °C ex vivo for 2-6 hours in RPMI (Gibco, 21875-034) containing 10% FCS (IGC Technical Services ...
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bioRxiv - Cell Biology 2022Quote: ... the cerebral cortices of 4 to 6 P0 rats were dissociated and chopped in Ca++/Mg++-free Hank’s Balanced Salt Solution (HBSS; Invitrogen, Carlsbad, CA). The tissue pieces were dissociated in phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2022Quote: We extracted total RNA from PFC and NAc tissues dissected from 6 rat brains using Trizol Reagent (Invitrogen, Cat, num. 15596018) and Zirconium Beads RNase Free (Next Advance ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR experiments were performed with SYBR Green Master Mix reagent with a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Relative quantifications were determined with the above primer pairs using delta delta threshold cycle methods with the following primers:
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bioRxiv - Cancer Biology 2022Quote: ... 250uL of strained sample (5% total marrow) was centrifuged at 600g for 6 minutes and resuspended in RNA Lysis/Binding Buffer (mirVana miRNA kit, Thermo Fisher) and stored at −80C ...
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bioRxiv - Microbiology 2022Quote: ... diluted 1:200 in blocking buffer for 30 min and mounted with ProLong Gold Antifade with DAPI (4′,6-diamidino-2- phenylindole; Thermo Fisher). Confocal laser micrographs were obtained with Zeiss LSM 880 laser microscope and analyzed with Zen black and Fiji software ...