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Citations for Thermo Fisher :
3351 - 3400 of 10000+ citations for 1 6 Bismaleimidoethane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... De-epidermized dermis were then placed at the bottom of a 6-well plate in 3D-HRPE culture medium composed of IMDM medium (Invitrogen) and keratinocytes medium (CellSystems ...
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bioRxiv - Microbiology 2019Quote: Total RNA was extracted from HIEs wells (in hW-CMGF+ or differentiation media for 4 days) or MA104 cells grown to confluency in a 6-well plate using TRIzol reagent (Ambion). Total RNA was treated with Turbo DNase I (Ambion ...
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bioRxiv - Systems Biology 2019Quote: ... for 30 minutes at 37C and plated with 2 million C57Bl/6 mouse embryonic fibroblasts as feeder cells (Gibco A34960) and with serum+LIF media composing of ...
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bioRxiv - Microbiology 2019Quote: ... Coverslips were mounted onto glass slides with ProLong Gold Anti-Fade reagent with DAPI (4[prime],6-diamidino-2-phenylindole; ThermoFisher). Images were obtained using an Olympus BX60 inverted microscope equipped with phase and fluorescence optics ...
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bioRxiv - Molecular Biology 2019Quote: ... 20ng of totalRNA was used for library preparation with ERCC Spike-in control Mix A (Ambion 10-6 final dilution). All steps were performed on a Tecan EVO150 ...
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bioRxiv - Genomics 2019Quote: ... A real-time PCR was performed using the QuantStudio 6 Flex Real-Time PCR System (# 4485694, Applied Biosystems, CA, USA) with the following reagents ...
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bioRxiv - Microbiology 2019Quote: ... pelleted again by centrifugation and resuspended at 3−5 × 106 cells/ml in sterile PBS containing 1.25 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; ThermoFisher Scientific) and stored on ice until required ...
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Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... 5 μl of each fraction (polysomes and non polysomes) was used for First-strand synthesis using p(dN)6 and 100 U of M-MLV RT (Invitrogen). The same amount of first strand DNA was used for qRT-PCR analysis ...
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bioRxiv - Genetics 2020Quote: ... we reverse transfected approximately 3.0 x 105 - 5.0 x 105 293T/TLR cells with 75 nM of siRNA per well of a 6-well plate using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Abi3-WT and -KO MØPs were maintained in 6-well plates with Roswell Park Memorial Institute 1640 (RPMI; Gibco®, ThermoFisher) supplemented with 10% heat-inactivated Foetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293 cells were grown on coverslips in 6 well dishes and transfected with pcDNA3.1-3XFLAG-ZNF507 plasmid using lipofectamine reagent (Thermo Fisher). After 36 hours cells were fixed with 4% paraformaldehyde in PBS for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... with 1.6 µM of specific primers with an QuantStudio™ 6 Flex system (Applied, Biosystems, Life Technologies, Saint-Aubin, France) in a final volume of 15µL ...
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bioRxiv - Cell Biology 2021Quote: ... For experiments glass-bottomed imaging dishes were exposed to deep UV light for 6 minutes and coated with Poly-D-Lysine (ThermoFisher) for a minimum of 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... was crosslinked to the PAAm gels using N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-SANPAH, from Life Technologies). 30 μL of 1mg/mL sulfo-SANPAH dissolved in milliQ H2O was added to PAAm gels which were irradiated under a 365nm UV lamp (ABM ...
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bioRxiv - Immunology 2021Quote: ... and primers and a FAM-ZEN/Iowa Black FQ labeled probe sequence (IDT) on the QuantStudio 6 system (Applied Biosystems). An Ultramer DNA oligo (IDT ...
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bioRxiv - Developmental Biology 2021Quote: ... The frozen sections were prepared from whole ovaries at 6 µm thickness and placed on charged glass slides (Fisher Scientific). Ovary sections were stained with hematoxylin and eosin (H&E ...
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bioRxiv - Biochemistry 2021Quote: ... HEK-293T cells were seeded onto 6-well culture plates and transiently transfected with different PTH2R constructs using Lipofectamine 2000 transfection reagent (Invitrogen). After 24 h ...
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bioRxiv - Developmental Biology 2020Quote: Mouse small intestinal organoids derived from the duodenum of C57BL/6 mice were cultured in ENR medium: Advanced DMEM/F12 (Invitrogen) with 1% Penicillin/Streptomycin (P/S ...
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bioRxiv - Neuroscience 2021Quote: ... Coverslips were washed 3 × 5 minutes in PBS and finally mounted onto glass slides using Prolong Gold mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) (Thermofisher). Images were acquired via confocal microscopy using a Zeiss LSM-880 confocal microscope (Plan-Apochromat 40x/1.3 Oil DIC UV-IR M27objective ...
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bioRxiv - Plant Biology 2021Quote: ... protoplasts were resuspended in lysis buffer (45mM magnesium chloride, 30mM sodium citrate, 20mM MOPS, 0.5% triton, 2% 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific), pH 7 ...
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bioRxiv - Microbiology 2021Quote: ... After 7 days of differentiation the BMDMs were seeded at a density of 0.8×106 cells/well in 6-well plates in MGM 10/10 media containing Dulbecco’s Modified Eagle Medium (DMEM) + Glutamax (Gibco®) containing 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... coverslips were mounted on slides using ProLong Gold Antifade Reagent with DAPI (4′,6-diamidino-2-phenylindole) (Thermo Fisher Scientific) and allowed to set overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were plated onto 6-well plates and transfected the following day (70-80% confluence) using Lipofectamine 2000 (LF2000) (Invitrogen) for 4 h in DMEM ...
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bioRxiv - Cell Biology 2020Quote: ... The production of ROS was assessed by the oxidation of 5-6-chloromethyl-2,7-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) (Invitrogen Corporation). Cells cultured in 96-well plates were incubated with JC1 ...
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bioRxiv - Cell Biology 2019Quote: ... in the iPSC media or in a feeder-free condition in StemFit AK02N (AJINOMOTO) in 6-cm dishes coated with 0.5 μg/cm2 vitronectin (Life Technologies). The cells were expanded using glass capillaries manually ...
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bioRxiv - Biophysics 2020Quote: ... with an N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The construct was transformed into bacterial DH10Bac component cells ...
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bioRxiv - Molecular Biology 2021Quote: ... or stress granules (G3BP1 antibody, Supplementary File 6) the cells were mounted along with 0.1 µm TetraSpeck™ microspheres (ThermoFisher) adhered to the slide according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 400 ng of each RNA was used following supplier’s recommendations and carried out on a Quant 6 RT PCR (Applied Biosystems). Gene expression data was analyzed by excel and normalized to the mock transfected values ...
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bioRxiv - Immunology 2021Quote: ... Real time PCR was carried out in triplicate on a QuantStudio 6 Flex instrument (Applied Biosystems, Foster City, CA, USA). Raw Ct values were first normalized using the inter-plate calibrator UniSp3 ...
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Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia functionbioRxiv - Molecular Biology 2020Quote: ... Protein samples either from ESCs or liver extracts were separated by SDS-PAGE and electroblotted onto nitrocellulose membranes (Whatman) using iblot System for 6 min (Invitrogen). Non-specific binding sites were blocked by incubation of the membrane with 5 % nonfat milk in PBS containing 0.1% Tween 20 (PBST) ...
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 in culture supernatant was quantified by commercial ELISA kits (Thermo Fisher Scientific, Waltham, MA, USA). The measurement of OD (Optical density ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was harvested 6 days following transfection and passed through a 0.45 μm filter prior to purification using CaptureSelect™ CH1-XL (ThermoFisher) affinity chromatography.
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bioRxiv - Immunology 2021Quote: The RBD was conjugated to CuMVTT using the cross-linker Succinimidyl 6-(beta-maleimidopropionamido) hexanoate (SMPH) (Thermo Fisher Scientific, MA) at 10-molar excess for 60 minutes at 23°C ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... 1-2 μg of plasmid was used to transfect 500,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5-10 μL; Thermo Fisher) in OptiMEM (400 μL ...
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bioRxiv - Cell Biology 2021Quote: HUVECs were plated in 6-well tissue culture plates and transfected with siRNA at a 50 nM concentration with RNAiMAX (ThermoFisher) using the protocol provider by the manufacturer ...
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bioRxiv - Genetics 2020Quote: ... then incubated for 2h at room temperature with Alexa Fluor secondary antibodies (S2 Table) and nuclear staining with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen). Images were captured on a BX51 fluorescence microscope (Olympus ...
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bioRxiv - Developmental Biology 2021Quote: ... Gene expression analysis was then performed by Real Time quantitative PCR (qPCR) using a QuantStudio 6 Flex thermocycler (Thermo Fisher). We used the following Taqman primers from Thermo Fisher (Supplementary Table 3) ...
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bioRxiv - Immunology 2021Quote: ... 293T cells were transduced as outlined above while being seeded at 20% confluency on either 6-well or 96-well plates (ThermoFisher). For 6-well plates ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we stained the formaldehyde preserves with DAPI (4’,6-diamidino-2-phenylindole, final concentration 5 ug/mL, Thermo Scientific, USA), and counted the microbial cells using hemocytometers (DHC-N01 ...
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bioRxiv - Microbiology 2020Quote: ... 2 μg of SOSIP trimer or 3.2 μg of SOSIP-I53-50NP (which is equivalent to 2 μg of trimer) were loaded on a 4-12% Tris-Glycine gel or 6-18% Tris-Glycine gel (both from Invitrogen). For BN-PAGE ...
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bioRxiv - Microbiology 2022Quote: Bone marrow cell suspensions were recovered from tibias and femurs of C57BL/6 mice in DMEM medium (Gibco, Life technologies) and cultured in medium complemented with mouse recombinant colony-stimulating factor 1 (mrCSF-1 ...
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bioRxiv - Microbiology 2022Quote: Bone marrow cell suspensions were recovered from tibias and femurs of C57BL/6 mice in DMEM medium (Gibco, Life technologies) and cultured in medium complemented with mouse recombinant colony-stimulating factor 1 (mrCSF-1 ...
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bioRxiv - Microbiology 2022Quote: ... for 30 minutes at RT followed by a 5-minute incubation with 300 nM 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... 24 hours before transfection 1.5 × 105 cells were seeded in a 6-wells plate with 3 mL of antibiotic-free medium (DMEM (Gibco™) high glucose ...
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bioRxiv - Neuroscience 2022Quote: ... or 300K cells per 6-well tissue culture plates were seeded in Microglia Differentiation medium (Advanced DMEM-F12 (Gibco, #12634010) + 1x N2 supplement (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... Ss06901209_m1), fibulin-5 (FBLN5, Ss04805024_m11) and samples were run on a QuantStudio 6 Real-Time PCR system (Applied Biosystems #4485694) in technical and biological replicates (n = 3-5) ...
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bioRxiv - Cancer Biology 2022Quote: ... The protein lysates were combined with 6× loading buffer containing 2-mercaptoethanol and loaded on NuPAGE 4%-12% Bis-Tris Midi gels (Invitrogen). After separation of protein by SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: ... Hoechst 43222 (H1399) and ProLong Gold Antifade Mountant with 4′,6- diamidino-2-phenylindole (DAPI; P36931) were purchased from Invitrogen.
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bioRxiv - Biophysics 2022Quote: Fibroblasts grown in vitro to the third passage were plated in 6-well tissue culture plates (5 × 105 cells per well) in DMEM (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum ...