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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... free floating slices were immunohistochemically stained for parvalbumin (Primary: anti-Parvalbumin, MilliporeSigma MAB1572, 1:500 dilution; Secondary: goat anti-mouse Alexa Fluor™ 568, ThermoFisher Scientific, 1:500 dilution). Brain sections were then mounted in Fluoromount (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... the mouse mNCK2 cDNA sequence was cloned via restriction enzymes in frame with a N-terminal 3XFLAG tag in pcDNA3.1(-) (ThermoFisher Scientific) for western blot (WB ...
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bioRxiv - Biochemistry 2024Quote: ... cell lysates were prepared as described above and subjected to immunoprecipitation with anti-DYKDDDDK-tag antibody clone 1E6 or mouse IgG anti-HA antibody clone 4B2 using Protein G Dynabeads (Thermo Fisher Scientific). The beads (bound fraction ...
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bioRxiv - Immunology 2021Quote: ... anti-Ollas tag (clone L2, Thermo Fisher Scientific), anti-S tag (clone D2K2V ...
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bioRxiv - Biophysics 2021Quote: ... The His-MBP tag was removed by cleavage with TEV protease (Invitrogen). Further purification was achieved through capture of the cleaved protein by Strep-Tactin resin (IBA ...
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bioRxiv - Microbiology 2021Quote: ... or His tag was purified using Protein G (cat.# 20399, Thermo Scientific) and Ni-NTA (cat.# R901100 ...
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bioRxiv - Immunology 2021Quote: ... were coated at 1 μg/mL 6x-His tag polyclonal antibody (Invitrogen) for >4 hours at ambient temperature and blocked ≥1 hour with 5% milk/1x PBS/0.01% Tween-20 at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Dynabeads™ His-Tag isolation and pulldown were purchased from Thermo Fisher Scientific.
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bioRxiv - Biophysics 2022Quote: ... blots were incubated with 6x-His Tag Monoclonal Antibody (Thermo Fisher Scientific). As a control ...
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bioRxiv - Biochemistry 2023Quote: TOPOVIBL452-579 was cloned as a His-tag fusion into pProEXHTb (Invitrogen). REC114-MEI4 complex and TOPOVIBL452-579 were individually expressed in E ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with 6x His-tag monoclonal primary antibody (MA1-135; ThermoFisher) overnight at a dilution of 1:750 ...
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bioRxiv - Immunology 2023Quote: ... and coated with 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) as previously described21 ...
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bioRxiv - Biochemistry 2024Quote: ... His-tag purification using Ni-NTA resin (Thermo Fisher, Massachusetts, United States) was performed and rubisco was eluted by SUMO tag cleavage with bdSUMO protease (as produced in Davidi et al ...
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bioRxiv - Immunology 2024Quote: ... were coated with 6x-His tag monoclonal antibody (HIS.H8) (ThermoFisher Scientific, Inc.) diluted in 0.1M sodium bicarbonate at 200 ng/well and incubated ON at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... an anti-6x His tag primary Mab (Thermo Fisher Scientific 4E3D10H2/E3) was introduced in 1:1000 dilution and incubated for one hour before further washes ...
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bioRxiv - Biochemistry 2024Quote: ... for 1 h followed by incubation with 1:2,000 diluted 6x-His tag monoclonal antibody (produced in mouse; Invitrogen; #MA1-21315) at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Ubiquitin B (UBB) in pENTR221 vector was purchased as an Ultimate ORF clone (Thermo Fisher Scientific, clone ID IOH56688), PCR amplified and inserted into pcDNA3.1-HA (gift from Oskar Laur (Addgene plasmid # 128034 ...
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bioRxiv - Immunology 2020Quote: ... cDNA was prepared using the Hi-Capacity cDNA Synthesis Kit (Applied Biosystems). For qPCR using RT4 PCR Profiler Arrays (Qiagen) ...
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bioRxiv - Cancer Biology 2021Quote: ... Inducible E-cad vector was generated by annealing the ORF-CDH1 (Clone ID:IOH46767, Invitrogen) cassette into an inducible backbone (pLIX403 ...
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bioRxiv - Microbiology 2022Quote: ... Mouse-anti-His (HIS.H8) antibody (Invitrogen) (for eGFP/His-tagged NP protein co-immunoprecipitation ...
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bioRxiv - Biochemistry 2021Quote: ... His (1:1000, Invitrogen, mouse monoclonal), Flag M2(1:1000 ...
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bioRxiv - Biochemistry 2024Quote: ... His-tagged wildtype or mutant SsdA was affinity-purified with Dynabeads™ His-Tag Isolation and Pulldown kit (ThermoFisher Scientific). The purified proteins were confirmed by SDS-PAGE and their concentrations were quantitated based on band intensities in SDS-PAGE using Image J40 against previously quantitated SsdA E348A as a standard.
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bioRxiv - Microbiology 2022Quote: ... A recombinant SPATR (rSPATR) fused to a polyhistidine tag (His-tag) was produced in BL21-CodonPlus-RIL competent cells (Invitrogen) and protein purification was performed under denaturing conditions using Ni-NTA Agarose (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-V5 tag (Invitrogen, R96025), rabbit anti-ZNF598 (Thermo Fischer Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... mouse monoclonal anti-his6 tag (Thermofisher) 1/10,000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... and into pVL847 (His-MBP-tag overexpression vector) by Gateway cloning method (Invitrogen). ΔnahA mutant of Bacillus subtilis was constructed by CRISPR/Cas9 editing method (Ref) ...
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bioRxiv - Immunology 2021Quote: ... plates were coated with 1 μg/mL 6x-His tag polyclonal antibody (Invitrogen) in 1x PBS for 6 hours at ambient temperature and blocked overnight with 0.5% NCS/5% Goat Serum/5% Milk/0.2% PBS-T ...
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bioRxiv - Cell Biology 2021Quote: ... before being incubated with the primary antibody (6x-His Tag Monoclonal Antibody, Invitrogen) overnight at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... the secondary antibody used was anti-6x-His tag monoclonal (Invitrogen, # MA1-21315) with 1:100 dilutions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 6x-His Epitope Tag Monoclonal Antibody (#MA1-21315, Thermofisher Scientific; dilution 1:500) was used for His-tag staining of the TRPA1 channel in fixed cells for 1 h at RT ...
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bioRxiv - Plant Biology 2024Quote: ... synthesized GUN1-FL protein was purified using His-Tag Dynabeads™ (Invitrogen; 10103D) and confirmed by Western blotting using His-tag antibody (Sigma ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μl of Dynabeads™ His-Tag Isolation and Pulldown beads (ThermoFisher 10104D) for each CFPS reaction were equilibrated twice in equal volumes of wash buffer before addition to the purified reactions to remove the Ulp-protease and incubated with spinning at 4 °C for at least 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Successful binding was analyzed using anti-His Tag antibody (PCRD, Invitrogen #MA1-135) or anti-Myc Tag (DCRD ...
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bioRxiv - Microbiology 2024Quote: ... or rabbit anti-6x-His-tag polyclonal antibody (Invitrogen™, Waltham, MA, USA), respectively ...
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bioRxiv - Biophysics 2024Quote: ... an amount of 10 µL per sample of magnet His-Tag Dynabeads (Invitrogen) were added ...
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bioRxiv - Biochemistry 2024Quote: ... and incubated with His-tag Isolation and Pulldown Dynabeads (ThermoFisher cat. No. 10103D) at room temperature for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... anti-Ollas tag-153Eu (clone L2, Thermo Fisher Scientific), anti-HSV tag-172Yb (rabbit polyclonal ...
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bioRxiv - Immunology 2021Quote: ... anti-Ollas tag-153Eu (clone L2, Thermo Fisher Scientific), anti-HSV tag-172Yb (rabbit polyclonal ...
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bioRxiv - Immunology 2021Quote: ... anti-V5 tag (clone R960-25, Thermo Fisher Scientific), anti-VSVg tag (rabbit pAb ...
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bioRxiv - Cell Biology 2022Quote: ... the c-Myc tag (Invitrogen, clone 9E10, MA1-980), or LRP6 (Cell Signaling ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight at 4°C with 2 ug/ml of mouse anti-His-tag antibody (Invitrogen cat. #MA1-21315-1MG, Thermo Fisher Scientific) in PBS ...
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bioRxiv - Synthetic Biology 2024Quote: ... 50 μL fractions were pelleted and resuspended in 200 μL of chilled PBSA containing 1 μg mL−1 primary antibody (Anti-His tag mouse antibody, Invitrogen, Catalog # MA121315 or Anti-GUS rabbit antibody, Invitrogen, Catalog # A5790) and incubated for 1 h ...
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bioRxiv - Neuroscience 2024Quote: The human DBT cDNA plasmid was obtained from the Ultimate ORF collection (Thermo Fisher). Synonymous mutations in the DBT cDNA were generated with Q5-site direct mutagenesis (NEB ...
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bioRxiv - Microbiology 2024Quote: ... pLenti-PACT-HA was generated by inserting PACT cDNA into a modified pLenti vector containing a HA-tag in the N-terminal using the Gateway Cloning System (Thermo Fisher Scientific). pLenti-PACTEA (K84E ...
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bioRxiv - Neuroscience 2024Quote: ... sheep anti-parvalbumin (PA5-47693, Invitrogen), goat anti-CD31 (AF3628 ...
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bioRxiv - Developmental Biology 2021Quote: ... The ORF of red fluorescent protein gene (RFP)-fused Antp was inserted into a pIZ/V5-His vector (Invitrogen) driven by the OpIE2 promoter ...
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bioRxiv - Microbiology 2022Quote: ... and expression clones with N-terminal fusion tags were produced simply by Gateway cloning (Gateway™ LR Clonase™ II Enzyme mix, Invitrogen, 11791020). The Lenti-NSP2 was constructed by using the destination vector ...
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bioRxiv - Immunology 2020Quote: ... PR8-HA and SARS-CoV-2 S proteins with C-terminal Avi-tags and His-tags were expressed via transient transfection of Expi293 suspension cultures (Thermo Fisher) and purified by polyhistidine-tag affinity and size exclusion chromatography ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was probed with primary antibodies including anti-tau mouse ET3 (1:1000 dilution) and 6X - His Tag rabbit monoclonal antibody (Invitrogen, 1:3500 dilution). The secondary antibodies used were ...
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bioRxiv - Microbiology 2021Quote: ... mouse anti-His (MA1-21315, Thermo Scientific), mouse anti-β-actin HRP (Santa Cruz Biotechnologies ...