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Citations for Thermo Fisher :
3401 - 3450 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Digested peptides were labeled using 11-plex or 16-plex tandem mass tag (TMT or TMTpro) reagents (Thermo Scientific), pooled ...
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bioRxiv - Synthetic Biology 2020Quote: ... Samples were then adjusted to 0.1% trifluoracetic acid and then desalted on Pierce peptide desalting spin columns (Thermo Scientific) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Peptides were concentrated on an Acclaim PepMap 100 C18 precolumn (75 μm x 2 cm, Thermo Scientific, Waltham, MA) and then were separated on an Acclaim PepMap RSLC column (75 μm x 25 cm ...
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bioRxiv - Microbiology 2019Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Biochemistry 2019Quote: ... Peptides were loaded with the autosampler directly onto a 2cm C18 PepMap pre-column (Thermo Scientific, San Jose, CA) which was attached to a 50cm EASY-Spray C18 column (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... Peptides were eluted from the column and into the source of a Q Exactive Plus mass spectrometer (Thermo Scientific) using the following program ...
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bioRxiv - Neuroscience 2019Quote: ... The antigen peptide with N-terminus Cysteine (NH2-CGGGGAAPGPPRHG-COOH) was coupled with keyhole limpet hemocyanin (Thermo fisher 77600). The antigen was injected into rabbit and antisera were collected after primary and several booster injections ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were labeled for 1 hour at room temperature using 11-plex tandem mass tag (TMT) reagents (Thermo Scientific) resuspended in 40% acetonitrile ...
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bioRxiv - Immunology 2020Quote: ... Digested peptides were individually subjected to LC-MS/MS with 2hr gradients on an Orbitrap Fusion Lumos Tribid (ThermoFisher). MS/MS was acquired in data-dependent precursor selection (see Table S1 for MS/MS spectral counts per run) ...
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bioRxiv - Genetics 2020Quote: ... Purification of antibodies from serum was performed by coupling the respective peptides to SulfoLink™ coupling resin (Thermo Scientific) and following the manuals instructions ...
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bioRxiv - Genetics 2020Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMap 100, Thermo Scientific) with buffer A (0.1% formic acid in DDW ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the peptides were analyzed by LC−MS/MS using a Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Scientific) connected in-line to an Easy-nLC HPLC system (Thermo Scientific) ...
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bioRxiv - Physiology 2021Quote: ... Acidified samples were desalted in C18 desalting columns (Pierce Peptide Desalting Spin Columns, Thermo Fisher Scientific, Rockford, IL, USA) and reconstituted in 95% HPLC grade water ...
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bioRxiv - Cell Biology 2020Quote: ... peptides were analyzed by online C18 nanoflow reversed-phase nano liquid chromatography (Dionex Ultimate 3000 RS LC, Thermo Scientific) combined with orbitrap mass spectrometer (Q Exactive Orbitrap ...
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bioRxiv - Biochemistry 2021Quote: ... The dried peptides from each sample were reconstituted in 0.1% formic acid and quantified in a NanoDropTM (Thermo Scientific) to equalize all samples at an identical protein concentration before analysis on the liquid chromatography-tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Biochemistry 2021Quote: ... The peptide–mRNA fusions were then incubated with human and yeast DUBm-immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... the trapped peptides were eluted onto a C18 column (nanoViper C18, 2 μm, 75 μm × 15 cm, Thermo Scientific) at a flow rate of 300 nL/min and subsequently separated with a 5-28% acetonitrile gradient with 0.1% formic acid for 80 minutes ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A’ (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Developmental Biology 2021Quote: ... and the next day peptides were labelled with TMT11plex reagents (0.4mg per sample) according to manufacturer’s instructions (Thermo Scientific). To quench the reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting peptides were analysed by liquid chromatography mass spectrometry using an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Eluting peptides were on-line ionized by nano-electrospray (nESI) using the Nanospray Flex Ion Source (Thermo Fisher Scientific) at 1.5 kV (liquid junction ...
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bioRxiv - Biophysics 2021Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Eluting peptides were on-line ionized by nano-electrospray (nESI) using the Nanospray Flex Ion Source (Thermo Fisher Scientific) at 1.5 kV (liquid junction ...
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bioRxiv - Molecular Biology 2020Quote: ... The peptides were separated and then analyzed using a Q Exactive Plus Hybrid Quadrupole-Orbitrap mass spectrometer (ThermoFisher Scientific). The resulting MS/MS data were processed using the Mascot search engine (version 2.3) ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Digested peptides were analyzed by LC-MS/MS over a 30-min gradient using LTQ XL-Orbitrap (Thermo Scientific) at the CentralProteomicsFacility (http://www.proteomics.ox.ac.uk ...
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bioRxiv - Immunology 2020Quote: ... 750,000 PBMCs were incubated either with DMSO (negative control) or with different peptide pools in 0.5 ml RPMI (Gibco) +10% Human AB serum (Sigma ...
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bioRxiv - Immunology 2021Quote: ... incubated beads were rotated with elution buffer (50 mM Tris, pH 7.5, 500 mM NaCl, and 10 mg/ml Myc peptide (M2435, ThermoFisher)) for 60 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Peptides were loaded onto an Acclaim PepMap100 nanoViper C18 trap column (100 µm inner diameter, 2 cm; Thermo Scientific) in HPLC buffer C with a constant flow of 10 µl/min ...
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bioRxiv - Immunology 2020Quote: 250 μL K92 peptide at 1 mg/mL was reduced with 6 μL of 0.5 M Dithiothreitol (Thermo Fisher) for 40 minutes at 37 °C and alkylated with addition of 18 μL of 2-Iodoacetamide (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted peptides were sprayed directly by electrospray ionization into the Q Exactive Plus Orbitrap mass spectrometer (Thermo Scientific). Mass spectra were acquired in data-dependent mode using a top10 sensitive method with one full scan (scan range ...
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bioRxiv - Cell Biology 2021Quote: ... Absolute abundances of Ub peptides were calculated by peak integration using Xcalibur Qual Browser (Version 2.2, Thermo Fisher Scientific). Layouts were applied according to the Supplementary Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Immunology 2022Quote: ... Peptides were loaded onto an Acclaim PepMap100 nanoViper C18 trap column (100 µm inner diameter, 2 cm; Thermo Scientific) in HPLC buffer C with a constant flow of 10 µl/min ...
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bioRxiv - Immunology 2022Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 10μg of peptide was prepared to label with TMTpro 16 plex reagents (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The peptides were eluted into the source of an Orbitrap Fusion™ Tribrid™ mass spectrometer (Thermo Fisher Scientific). The spray voltage was set to 2.25 kV and the temperature of the heated capillary was set to 280°C ...
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bioRxiv - Biochemistry 2022Quote: ... The peptides were eluted into the source of an Orbitrap Eclipse™ Tribrid™ mass spectrometer (Thermo Fisher Scientific). The spray voltage was set to 2.25 kV and the temperature of the heated capillary was set to 275°C ...
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bioRxiv - Cell Biology 2022Quote: ... peptides in 1 % (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5 % (vol/vol ...
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bioRxiv - Immunology 2022Quote: ... The resulting tryptic peptides were subsequently labelled using TMT-6plex Isobaric Mass Tagging Kit (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s instructions as follows ...
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bioRxiv - Microbiology 2022Quote: LC-MS analysis of linear peptides (unmodified and phosphopeptides) was performed using a Dionex UltiMate 3000 system (Thermo Fisher) connected to a PepMap C-18 trap-column (0.075 x 50 mm ...
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bioRxiv - Microbiology 2022Quote: ... Digested peptide mixtures were analyzed by LC-MS/MS on an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific) equipped with an Easy-nLC 1200 HPLC (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2022Quote: Peptides from each sample were analyzed on an Orbitrap HF-X mass spectrometer (Thermo Fisher Scientific, San Jose, CA) using an overlapping window data-independent analysis (DIA ...
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bioRxiv - Plant Biology 2022Quote: ... the peptide identification was performed by searching the Arabidopsis thaliana reference genome (downloaded from https://www.uniprot.org) using SEQUEST (ThermoFisher Scientific) search engine ...
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bioRxiv - Plant Biology 2022Quote: ... Pooled samples were subjected to high pH fractionation using Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific) following manufacturer instructions ...
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bioRxiv - Genomics 2022Quote: ... tryptic peptides (1 mg) were decomplexed by reversed phase nanoLC separation (Ultimate 3000 nanoRSLC; Thermo Fisher Scientific, Dreieich, Germany) using a trap column setup (2 cm length ...
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bioRxiv - Microbiology 2022Quote: ... LC-MS/MS analysis of digested peptides was performed on an Orbitrap Eclipse mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... the resulting peptides were quantified via absorbance measurements at 205 nm using a NanoDrop 2000c spectrophotometer (Thermo Fisher Scientific) and then injected into a NanoAcquity ultra-performance LC coupled to a Q-TOF SYNAPT G2-Si mass spectrometer (Waters ...
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bioRxiv - Microbiology 2023Quote: ... The peptide concentrations were determined by BCA assay using the Pierce Rapid Gold BCA Protein Assay Kit (ThermoFisher Scientific), and the protein concentration was determined by UV absorbance at 280 nm ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; NanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...