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Citations for Thermo Fisher :
3201 - 3250 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... Slides were then washed with PBS-T (3 × 5 min) and incubated with fluorophore-conjugated secondary antibodies (1:500 in blocking buffer, Invitrogen). Hoechst 33258 (Invitrogen ...
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bioRxiv - Cell Biology 2019Quote: ... the Click reaction is performed after blocking (if photobleaching is required, Click reaction also is performed after) following manufacturer’s protocol (Invitrogen, #C10269).
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bioRxiv - Physiology 2021Quote: ... using goat anti-rabbit-Alexa Fluor488 and goat anti-mouse-Alexa Fluor568 secondary antibodies (4 μg/ml in blocking buffer; Invitrogen) and DAPI (1 μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... incubated with blocking solution (2% FBS in PBS) for 30 minutes and incubated with Alexa-594 conjugated goat anti-mouse IgG (Invitrogen) diluted 1:200 in blocking solution ...
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bioRxiv - Neuroscience 2019Quote: ... The fixed cells were then pre-blocked for a minimum of 30 minutes in a blocking solution of KPBS with 0.25% triton-X100 (Fisher Scientific) and 5% donkey serum ...
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bioRxiv - Systems Biology 2019Quote: ... sections were washed with blocking solution and incubated with DAPI (1 μg/ml in PBS, Thermo Fisher Scientific Inc.; D1306) to stain DNA for 10 min at RT before they were covered with coverslips using Fluoromount-G® (SouthernBiotech ...
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bioRxiv - Neuroscience 2021Quote: ... slices were washed 3 times in PBS and incubated for a further hour at room temperature in diluted blocking solution containing 1:500 goat anti-mouse Alexa488-conjugated IgG secondary antibody (#A28175, RRID:AB_2536161, Invitrogen, Carlsbad, CA) and 1:5000 Hoechst stain (Thermo Scientific ...
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bioRxiv - Genetics 2020Quote: ... The fixed cells were then pre-blocked for a minimum of 30 minutes in a blocking solution of KPBS with 0.25% triton-X100 (Fisher Scientific) and 5% donkey serum ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary labeling was performed by addition of the following antibodies diluted at 1:500 in blocking/ permeabilization buffer for 20 minutes at room temperature: Alexa Fluor 555 or 488 donkey anti-mouse (Invitrogen), Alexa Fluor 555 or 488 donkey anti-rabbit (Invitrogen) ...
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bioRxiv - Zoology 2021Quote: ... specimens were rinsed twice with the blocking solution and treated with the secondary antibodies (Donkey anti-Mouse IgG Alexa Fluor 488, ThermoFisher Scientific A21202 and Donkey anti-Rabbit IgG Alexa Fluor 647 ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with phosphate buffered saline with 0.05% Tween (PBS-T) buffer and blocked with TBS Startblock blocking buffer (ThermoFisher, Scientific). Histidine-tagged hACE2 and hDPP4 receptors were 3-fold serially diluted (5-0.0001 μg mL−1 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed 3 times in the blocking solution and incubated in Alexa Fluor 594-conjugated (red) goat secondary antibody (1:500; Invitrogen) for 2 hr.
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bioRxiv - Immunology 2020Quote: ... and blocked with 200 µL blocking buffer (TBST-MS: 1x TBST + 1% w/v milk, 1% v/v goat serum (Gibco) for 1 h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Goat anti-hamster IgG (H+L) HRP conjugated secondary antibody (SeraCare 5220-0371) was diluted 1:3,000 in casein in PBS blocking buffer (Thermo Fisher), and 100 μL was added to the wells and allowed to incubate for one hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Plates were then washed three times with PBS + 0.1% Tween-20 (PBST) and blocked with 100 μL casein in PBS blocking buffer (Thermo Fisher) for one hour at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were rinsed with PBS and incubated in blocking solution containing appropriate secondary antibodies conjugated to an Alexa-Fluor (4μg/mL, Thermo Fisher) and costained with DAPI (1μg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... Microtubules were then stained using a rat anti-tubulin primary antibody solution (diluted 1:500 in blocking/permeabilization buffer, MA1-80017, Invitrogen) with a 4°C overnight incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were rinsed with PBS-TB (PBS supplemented with 0,05% Tween and 1% BSA) and incubated for 30 minutes with 200μl Superblock blocking buffer (Thermo Scientific, #37515). Antibody incubation was split into two steps ...
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bioRxiv - Cell Biology 2022Quote: ... The next day the fibers were washed 3×10 min in PBS containing 0.04% saponin and incubated in blocking buffer with Alexa 488 anti rabbit or Alexa 568 anti rabbit or anti mouse (Invitrogen) for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... The sections were blocked in blocking buffer for 1h at room temperature and incubated with BODIPY 558/568 (Invitrogen, D38D35) for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... sections were washed and incubated for 1 h with Alexa Fluor conjugated secondary antibodies (1:1000 in blocking solution; Invitrogen). Following extensive washing ...
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bioRxiv - Cell Biology 2022Quote: ... The following day slides were washed thrice with blocking buffer for 15 min and incubated for 1 h at RT in secondary antibodies and Hoechst 33342 (Invitrogen) diluted in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were incubated with fluorophore-conjugated secondary antibodies in blocking solution for 1 hr (1:200, Alexa 488 or 555, Invitrogen) then mounted with DAPI-containing Fluoromount medium (SouthernBiotech) ...
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bioRxiv - Cell Biology 2024Quote: ... Non-specific binding was inhibited by incubating for 2 h in blocking buffer (5% BSA or Goat Serum (Invitrogen, #500622) + Triton X-100 (VWR ...
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bioRxiv - Physiology 2022Quote: ... Membranes were blocked using Intercept (PBS) Blocking Buffer and incubated overnight in the following primary antibodies: rabbit PDK4 (PA5-13776) from ThermoFisher; goat PGC-1α (ab106814) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed in blocking buffer with Hoechst nuclear stain (1:10,000) and mounted with Prolong Gold Antifade (Thermo Fisher).
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bioRxiv - Neuroscience 2022Quote: ... The slices were treated with 0.5% Triton X-100 in blocking solution (10% (v/v) horse serum (Thermo Fisher Scientific), 5% (w/v ...
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bioRxiv - Neuroscience 2022Quote: ... Collected sections were mounted on glass slides, blocked in blocking buffer (5% goat serum, 0.3% Triton X-100 in PBS) (all materials here from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the coverslips were washed three times in PBS with 0.1% saponin and incubated for one hour at room temperature in blocking solution with 1.6 μg/ml DAPI dilactate (Molecular Probes, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Embryos were rehydrated to 1x PBS with 0.1% Triton X-100 (PBT) and blocking was performed for 2h in 10% CAS-Block / 90% PBT (Life Technologies). Staining was performed using the Atp1a1 antibody (1:200 ...
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bioRxiv - Biophysics 2024Quote: ... the sample was transferred into a tube previously passivated with denatured BSA blocking buffer and mixed with magnetic beads (Dynabeads M-270 Streptavidin, Invitrogen) coated with DNA strands complementary to a section of the purification linker handles on the triangles ...
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bioRxiv - Neuroscience 2024Quote: ... Brain sections were washed three times for 5 minutes in PBS and incubated for 1 hour in blocking solution [10% normal goat serum (NGS; #50197Z, Invitrogen), 0.1% Triton X-100 in PBS] ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were washed extensively in PBS and then incubated overnight at 4°C in blocking solution with the appropriate concentration of secondary antibodies diluted at 1:500 and with DAPI (Invitrogen) diluted at 1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... blocking with 5% heat-inactivated sheep serum and incubation with an anti-digoxigenin antibody conjugated to alkaline phosphatase (Invitrogen, AM1326). NBT/BCIP (4-nitro blue tetrazolium chloride/5-bromo-4-chloro-3-indolylphosphate ...
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bioRxiv - Immunology 2024Quote: ... followed by incubation with blocking solution (Goat serum, dilution 5:100 in Phosphate Buffered Saline Tween20,PBST, 0.05%, Fisher Scientific) for 1hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The membranes were placed in an opaque gel box and incubated with blocking buffer (PBST + 10% Fish gelatin (PBS 10X, pH 7.2, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed with permeabilization/blocking solution and stained with a 1:400 dilution of Alexa488-conjugated anti-mouse antibody (Invitrogen) for 1h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... sections were then incubated for 2h at RT in the same blocking solution containing 1:1000 dilutions of corresponding Alexa secondary antibodies (Invitrogen) made in goat and coupled to either 488 ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated in primary antibody diluted in blocking buffer using the following primary antibodies: anti-Phospho-Tau (Ser396) (1:500, 44-752G, Invitrogen), anti β-catenin (E-5 ...
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bioRxiv - Molecular Biology 2023Quote: ... and once with 1X PBS for 5 min each before blocking with 100 mM N-succinimidyl (acetylthio) acetate (NHS-acetate, ThermoFisher) in NHS-acetate buffer (0.1M NaP ...
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bioRxiv - Cell Biology 2023Quote: ... After blocking with 1% normal donkey serum (NDS, Biozol, END9010) and 1% bovine serum albumin (BSA, Thermo Fisher Scientific, 23209) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... in blocking buffer overnight in 4°C and stained with secondary AlexaFluor633 donkey anti-goat antibodies (Invitrogen, #A21082; 1:200) for 1 hour at RT ...
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bioRxiv - Biophysics 2022Quote: ... the tissues were washed with blocking solution three times and incubated with the respective secondary antibody (polyclonal goat anti-mouse IgG1, 1:500, Invitrogen) for 45 min at room temperature ...
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bioRxiv - Genetics 2022Quote: ... we incubated the samples in blocking solution with fluorescent dye-conjugated secondary antibodies and the nuclear counterstain DAPI (ThermoFisher Scientific) at 4 °C overnight ...
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bioRxiv - Immunology 2023Quote: ... the pellet was resuspended in 90 μl of Secondary antibody solution (Blocking buffer + 1:500 Goat anti-rabbit IgG AlexaFluor 647 antibody [#A27040, Invitrogen]) and the samples were incubated at room temperature for 45 min in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... The sample was washed in PBT for 3 hr at 25 °C then incubated in the secondary antibody AF488-conjugated goat-anti-mouse (1:500 in blocking buffer, Ref# A21202, Invitrogen) at 4 °C for 24 hr ...
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bioRxiv - Cell Biology 2023Quote: ... the wells were washed 4 times with blocking buffer and incubated with 100 µl TMB substrate solution (Thermo Scientific; #N301). After 20 minutes of incubation at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... in PBT then incubated for 24–48 hr at 4 °C in the primary antibody mouse anti-GFP (1:500 in blocking buffer, Ref# A11120, Invitrogen). The sample was washed in PBT for 3 hr at 25 °C then incubated in the secondary antibody AF488-conjugated goat-anti-mouse (1:500 in blocking buffer ...
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bioRxiv - Systems Biology 2023Quote: ... primary antibodies were directly added to the floating sections in the blocking buffer in the following concentrations: rabbit anti-NeuN antibody (Invitrogen) for neuron at 1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated in a biotinylated goat anti-rabbit secondary antibody in blocking solution (Invitrogen, cat # 31820, 1:500) and transferred in a detection reagent (Vectastain Elite ABC kit ...