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Citations for Thermo Fisher :
3251 - 3300 of 5130 citations for RFC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... 200 μl of blocking buffer containing a 1:1000 dilution of goat anti-rabbit Alexa 488 (Thermo Fisher; catalog #R37116) was added to the well and samples were incubated overnight on a rocking incubator at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... retinas were incubated on a rocking platform for 2 hours at room temperature (RT) in Claudio’s blocking buffer (CBB) consisting of 1% FBS (LTID 10500-064, Thermo Fisher), 3% BSA (MB04602 ...
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bioRxiv - Neuroscience 2023Quote: ... then washed with PBS and secondary antibody in blocking solution (Alexa Fluor™ 594 donkey anti-rabbit IgG, ThermoFisher, R37119) was added (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed three times with 1× PBS for 5 minutes each and blocked for 30 minutes at room temperature with Blocking Buffer [0.4% acetylated BSA (Ambion, AM2614) in 4× SSC] in a humidified chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... and probed with an Alexa Fluor 488-conjugated goat-anti-rabbit secondary antibody diluted in blocking buffer (1:100, #A11008, Invitrogen) for 1 h at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The slides were then rinsed 4 times with the washing buffer for 15 minutes and incubated with the secondary antibody at 1:500 dilution in blocking solution for 1h at room temperature (Rabbit 555, Invitrogen, #AB_162543 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were transferred to blocking solution containing 1:1000 primary antibody rabbit Polyclonal anti-GFAP (PA5-16291, ThermoFisher Scientific) for 24 hours at 4 °C ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... Incubation was performed for 1 h at RT before washing three times with blocking solution and mounting the slides in ProLong Gold antifade reagent with DAPI (Invitrogen). Images were obtained by the LSM 980 AIRY confocal microscope (Zeiss) ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... cells were washed three times with blocking solution before staining with secondary antibodies Alexa Fluor 488 goat anti-mouse IgG (A11001, Invitrogen) and Alexa Fluor 568 goat anti-rabbit IgG (A110011 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 2 h with secondary antibodies diluted in blocking buffer to 1:600 (donkey anti-rabbit IgG Alexa Fluor 488, Invitrogen, Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated in a biotinylated goat anti-rabbit secondary antibody in blocking solution (Invitrogen, cat # 31820, 1:500) and transferred in a detection reagent (Vecstatin Elite ABC kit ...
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bioRxiv - Microbiology 2023Quote: ... Monolayers were washed three times with blocking buffer before incubation in the dark with a 1:1000 dilution of Alexa546-conjugated anti-rabbit IgG (ThermoFisher) for 1 hour at 23ºC ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were successively incubated with primary antibodies diluted in blocking buffer (mouse anti-G3BP1, 1:4000, BD Biosciences, rabbit anti-GFP, 1:1000, ThermoFisher, mouse anti-PKR ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed twice in 0.01 M PBS for 10 min followed by a 1 hr incubation in a blocking solution comprised of 0.3% Triton X-100 (Fisher Scientific) and 1% Bovine Serum Albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... at RT for 10 min and blocked at RT for 1 hr in blocking solution (5% NBCS, 2x antibiotic-antimycotic (Gibco), 0.1% sodium azide (Sigma s2002-100G) ...
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bioRxiv - Microbiology 2023Quote: ... the membranes were incubated with an anti-TasA antibody (rabbit) at a 1:20,000 dilution in Pierce Protein-Free (TBS) blocking buffer (Thermo Fisher). A secondary anti-rabbit IgG antibody conjugated to horseradish peroxidase (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... followed and the slides were incubated with secondary antibody (1:400 in blocking buffer, donkey a-rabbit Alexa 488 antibody, Invitrogen A-21206 ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then washed with 1X Ca2+ and Mg2+-free PBS prior to blocking with 5% normal goat serum (NGS, Thermofisher) in 1% PBS/Bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed 4 times with 300ul blocking solution (15 minutes each) and incubated with secondary antibodies diluted in blocking solution (goat anti-mouse conjugated with AlexaFluor 555 (ThermoFisher) and goat anti-guinea-pig conjugated with AlexFluor 647 or goat anti-rabbit conjugated with AlexaFluor 647 ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were incubated in secondary antibodies in blocking buffer for 1 hour at room temperature in the dark (Alexa Fluor 488 goat anti-chicken, Invitrogen A-11006 ...
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bioRxiv - Cancer Biology 2024Quote: ... antigen retrieval was performed using citrate (pH 6.0) or Tris-EDTA buffer as previously described prior to incubation with BlockAid Blocking Solution (Thermo Fisher). For IHC ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with PBS and incubated in blocking buffer for 1 h with Streptavidin-AlexaFluor 488 (S11223, Molecular Probes), goat anti-Mouse IgG-AlexaFluor 555 (A-21424) ...
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bioRxiv - Bioengineering 2023Quote: ... The resulting cell pellet was suspended in a blocking solution consisting of FACS buffer (1X PBS with 5% fetal bovine serum (Gibco) and 0.1% sodium azide ...
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bioRxiv - Cell Biology 2023Quote: ... ALFA-FAM134s were targeted directly using an anti-ALFA nanobody carrying a 5xR1 docking strand (5-TCCTCCTCCTCCTCCTCCT-3’) (Massive-TAG-Q Anti-ALFA Custom, Massive Photonics, GmbH) which was diluted in permeabilization/blocking buffer (10% FCS (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies rabbit anti-HA (1:500) was subsequently added in permeabilization/blocking buffer (10% fetal calf serum (FCS) (Gibco), 0.1% saponin (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2023Quote: ... samples were permeabilized and blocked in blocking buffer (PBS+0.1% Triton-X100 plus 3% BSA and the Image-IT reagent (Thermo Scientific)) ...
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bioRxiv - Biochemistry 2024Quote: ... cells were permeabilized and blocked in 0.22 µm filter-sterilized 1X blocking buffer (10% normal goat serum (Thermo Scientific; 50197Z), 0.3% Triton X-100 ...
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bioRxiv - Bioengineering 2023Quote: ... the gels were washed with wash buffer three times for 5 min followed by permeabilization and blocking in 5% goat serum (Gibco), 1% BSA ...
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bioRxiv - Immunology 2024Quote: ... The wells were then preincubated with either 200µl of BSA blocking buffer (Thermo Scientific; 5% bovine serum albumin in PBS) for total binding (TB ...
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bioRxiv - Cancer Biology 2024Quote: ... Followed by two more PBS rinses prior to 1 hour incubation with SuperBlock™ Blocking Buffer (Thermo Scientific™,37515) for blocking non-specific antibody binding ...
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Deep brain stimulation induces white matter remodeling and functional changes to brain-wide networksbioRxiv - Neuroscience 2024Quote: ... these sections were incubated for 24 h with blocking solution (5% normal goat serum (Cat #PI31873, Invitrogen, Waltham, MA, USA) and diluted with PBS solution containing 0.3% Triton X-100 (TX-100) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were rinsed 1-2 times with 1X PBS and incubated for 2 hours in Alexa Fluor conjugated goat anti-rabbit/ chicken or donkey anti-goat/rabbit secondary antibodies along with DAPI (dilution 1:1000 X in blocking buffer) (Invitrogen) at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... followed by a 1-hour incubation in secondary antibody (diluted in blocking buffer, 1:1000, Alexa Fluor 488 donkey anti-goat (Invitrogen), 1:1000 Alexa Fluor 488 donkey anti-rabbit (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated 1 hour in blocking solution and incubated at 4 °C overnight with secondary antibodies conjugated with AlexaFluor (Molecular probes) diluted in blocking solution ...
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bioRxiv - Physiology 2024Quote: ... Cells were then immunostained using CDH5 antibody [36] diluted 1:100 in blocking buffer for 30 min followed by Alexa Fluor 555–conjugated goat anti–rabbit antibodies (ThermoFisher) diluted 1:200 in blocking buffer together with Hoechst ...
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bioRxiv - Immunology 2024Quote: ... for 1.5 hours (100 V constant, 4°C).The membrane was transferred into SuperBlock T20 (TBS) blocking buffer (Thermo Scientific) and blocked overnight while shaking at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... We then incubated membranes with secondary antibody (in 5% Blocking Buffer) for 1 hour at room temperature (Invitrogen cat. A16110; Invitrogen cat ...
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bioRxiv - Cell Biology 2024Quote: ... Microtubules were then stained using the rat anti-tubulin primary antibody (1:500 in blocking/permeabilization buffer, MA1-80017, Invitrogen) with a 4 °C overnight incubation ...
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bioRxiv - Neuroscience 2024Quote: ... and revealed with appropriate Alexa fluorophore-conjugated secondary antibodies (1:1000 in blocking buffer, A11013 and A21429, Thermofisher, Courtaboeuf, France) and DAPI (10 µg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... Then secondary antibodies were then added in blocking buffer over night at 4 ºC (Goat anti Rabbit 647, Thermo Fisher Scientific A21245 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were also labeled with phalloidin conjugated to Alexa Fluor 594 in blocking buffer according to manufacturer protocol (Thermofisher A30107). After incubation with DAPI for 5 min and subsequent PBS wash ...
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bioRxiv - Genomics 2024Quote: ... and pellets were resuspended in ice-cold bovine serum albumin (BSA) blocking solution (1× phosphate-buffered saline (PBS) (AM9625, Ambion), 0.5% BSA (VWR ...
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bioRxiv - Biophysics 2020Quote: ... 1 nmol of biotinylated 4-heptad CTD peptides (PeptideSynthetics) were incubated with 0.5 mg of Streptavidin-coupled magnetic Dynabeads M-280 (Invitrogen) in 300 µL of buffer A (25mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: Peptide fractions were analysed by nanoflow HPLC-MS/MS using an Ultimate 3000 nano RSLC system (Thermo Fisher Scientific) coupled with a Q-Exactive HF mass spectrometer fitted with a nano spray Flex ion source (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... the peptides were analyzed using nano flow reverse phase liquid chromatography on a Proxeon Easy-nLC 1000 (Thermo Scientific) coupled to an Orbitrap Elite (Thermo ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were separated using an analytical C18 Acclaim PepMap column (75 µm × 250 mm, 2 µm vesicles; Thermo Scientific) and a 180-min gradient at a flow rate of 300 µl/min ...
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bioRxiv - Cell Biology 2019Quote: Fractionated peptides were separated by nanoscale C18 reverse-phase liquid chromatography using an EASY-nLC II 1200 (Thermo Scientific) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide yield from each sample was determined to be approximately 20 µg based on BCA Protein Assay (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... was used to elute peptides from the capillary reverse-phase column (0.075 × 500 mm. Pepmap®. Thermo Fisher Scientific). Data were acquired using the Xcalibur 4.0 software ...
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bioRxiv - Cell Biology 2020Quote: ... The digested peptides were dried in a SpeedVac and labelled with TMT10plex as instructed by the manufacturer (Thermo Fisher). Labeled peptides were mixed ...