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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... LC mobile phase solvents and sample dilutions used 0.1% formic acid in water (Buffer A) and 0.1% formic acid in 80% acetonitrile (Buffer B) (Optima™ LC/MS, ThermoFisher). Data acquisition was performed using the instrument supplied Xcalibur™ (version 4.1 ...
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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bioRxiv - Molecular Biology 2020Quote: Single- and double-stranded oligonucleotide substrates were diluted in TBST with 0.1% BSA and 3 mM magnesium chloride to 1 μM final concentrations Previously mentioned enzymes and RNase A (EN0531, Thermo Fisher) were used at a 1:200 final dilution and incubated with indicated nucleic acid substrates for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were washed three times with PBS and incubated with DAPI for 5 min (1:5,000 in PBS ...
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bioRxiv - Microbiology 2021Quote: ... the monolayers were overlaid with 3 mL containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2022Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were dry-mounted on slides using Fluoromount (Sigma ...
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bioRxiv - Microbiology 2020Quote: ... and were transferred into tubes containing 3 μl of RNase inhibitor solution (1 part 20 U μl−1 RNase Inhibitor [Thermo Fisher Scientific] in 19 parts 0.2% Triton X-100 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Neuroscience 2021Quote: ... Sections were rinsed 3 times in PBS and incubated for 1 h at room temperature with corresponding secondary antibodies (1:500, Life Technologies). Sections were washed three times with PBS and incubated with DAPI for 5 min (1:5,000 in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... The barrier function of the Calu-3 monolayer was terminally evaluated via immunofluorescence imaging of tight junction protein 1 (ZO-1 antibody, Invitrogen, 339194) and permeability assays using 500 µL of 2 mg/mL FITC-dextran (4 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... cryoprotected in 30% sucrose solution (for 1 to 3 days) and incubated with AF488 streptavidin (1:1000, Invitrogen, Cat#: S11223, 2390711) in PBS containing 0.3% Triton X-100 (Aladdin ...
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bioRxiv - Physiology 2023Quote: ... followed by 1 hour incubation in DMEM/3% bovine serum albumin containing 1 μM AlexaFluor 647 conjugated α-bungarotoxin (Life Technologies) at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... KIF18A siRNAs used were a 1:1 mixture of two the following Silencer or Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334), GCUUGUUCCAGAAUCGAGAtt (Ambion ...
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bioRxiv - Immunology 2023Quote: ... cDNA was diluted 1:3 or 1:4 before mixing with Power SYBR Green PCR Master Mix (CAS: 4368702, Applied Biosystems) and primer pairs (Table S4B) ...
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bioRxiv - Genomics 2022Quote: ... Samples were washed 3 times with methanol before resuspension in 1 ml PBT containing 1 µL Hoeschst 33342 (Thermo Scientific, USA). After a 10 min incubation at RT ...
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bioRxiv - Microbiology 2022Quote: ... and monolayers were overlaid with 3 ml containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibody labeling was done at 1:1000 dilution in 3% goat serum/PBST at room temperature for 1 hr using goat-anti-chicken-AF488 (ThermoFisher A11011). After 1 hr incubation ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibody labeling was done at 1:1000 dilution in 3% goat serum/PBST at room temperature for 1 hr using goat-anti-chicken-AF488 (ThermoFisher A11011), goat-anti-rabbit-AF568 (ThermoFisher A11036) ...
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bioRxiv - Cell Biology 2022Quote: ... HCEnCs were rinsed in DPBS and passaged at a ratio of 1:2 or 1:3 with TrypLE (Thermo Fisher Scientific) for 10-15 min at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... KIF18A siRNA used was a 1:1 mixture of the following two Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334) CGUUAACUGCAGACGUAAAtt (Ambion ...
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bioRxiv - Microbiology 2023Quote: ... and monolayers were overlaid with 3 ml containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Cell Biology 2024Quote: ... suspended in arrest-mixture assay buffer (no ATP) with 3% BSA and 1:400 anti-CARMIL1 antibody (ThermoFisher, 27133-1-AP). After incubation for 2 hr at RT ...
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bioRxiv - Cancer Biology 2024Quote: ... these plasmids were transfected into HEK293T cells alongside psPAX2 and pMD2.G at a mass ratio of 1 pMD2.G : 3 psPAX2 : 4 pLKO.1 using Lipofectamine 2000 (Invitrogen 11668019), and media containing the lentivirus was concentrated with Lenti-X Concentrator (Takara Bio 631232 ...
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bioRxiv - Bioengineering 2024Quote: ... 80-90 % of cell cultures were split into a ratio of 1/3 to 1/6 using Tryple Express Enzyme (Gibco, 12604021) and Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Biophysics 2021Quote: ... solution (pH 8) and incubated in 0.1M sodium bicarbonate solution (pH 8) with 2 uM Alexa Fluor™ 647 NHS Ester (Invitrogen, A37573) for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... at a 1.5 fold molar surplus and then incubated for 2 hours followed by an incubation with 1.5-fold excess Alexa Fluor 647 succinimidyl ester (Thermo Fisher Scientific) in the presence of 330 mM NaHCO3 for 2 hours at room temperature ...
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bioRxiv - Immunology 2022Quote: ... PBMCs obtained from various time points were pre-labelled with 5 µM of carboxyfluorescein diacetate succinimidyl ester (CFSE; Thermo Fisher Scientific) in PBS with 2.5% FBS for 8 minutes in 37°C water bath ...
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bioRxiv - Biophysics 2020Quote: Water soluble dye and conjugated proteins were prepared according to the manufactures instructions and added to tGPMV and GPMV solution after isolation from the cell culture in the following final concentrations: Alexa Fluor 647 NHS-Ester (Thermo Fisher) at 400 μM ...
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bioRxiv - Immunology 2021Quote: ... domain of the S-protein were labeled with 4 different fluorophores (Alexa Fluor 647, 488, 594 and 405) using Alexa Fluor NHS Ester kit (Thermo Scientific), were mixed and incubated with S-protein-beads ...
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bioRxiv - Biophysics 2021Quote: ... purified NHR2 protein was labeled either with the dye Alexa Fluor® 488 (NHS Ester Protein Labeling kit, ThermoFisher Scientific, USA) or Monolith NT™ (Protein Labeling Kit Blue-NHS ...
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bioRxiv - Biochemistry 2020Quote: ... Covalent labeling of amine groups of the proteins with Alexa Fluor 488 or Alexa Fluor 647 succinimidyl ester dyes (Molecular probes/Invitrogen) was conducted in its polymer-assembled form as previously described (González et al ...
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bioRxiv - Cell Biology 2021Quote: ... All gels excluding samples that were processed for subsequent NHS ester staining were transferred to a 12-well plate and digested with 7.5 U/mL Proteinase-K (Thermo Fisher, EO0491) in TAE buffer (containing 40 mM Tris ...
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bioRxiv - Systems Biology 2020Quote: ... The 5’ amine-modified DNA oligonucleotides (Integrated DNA Technologies) with the readout probe sequences were conjugated to Alexa Fluor 647-NHS ester (Invitrogen A20006) or Cy3B-NHS ester (GE Healthcare PA63101 ...
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bioRxiv - Systems Biology 2021Quote: ... Integrated DNA Technologies) of 12-15-nt in length were designed and conjugated to Alexa Fluor 647-NHS ester (Invitrogen A20006) or Cy3B-NHS ester (GE Healthcare PA63101 ...
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bioRxiv - Cell Biology 2020Quote: ... Antibodies formulated in PBS only were concentrated using 0.5 mL 50KDa Amicon Ultra Filters (EMDMillipore, UFC510096) to 2 mg/ml and reacted with maleimide-PEG2-succinimidyl ester crosslinkers (ThermoFisher 22102) for 2 hours at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... were carboxy-coupled to magnetic Luminex microplex carboxylated beads (Luminex Corporation) using NHS-ester linkages with Sulfo-NHS and EDC (Thermo Fisher) and then incubated with serum (IgG1 ...
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bioRxiv - Bioengineering 2022Quote: ... The excess unreacted DBCO-NHS ester was removed from the activated Ab with an equilibrated Zeba Spin Desalting Column (7k MWCO, Thermo Scientific). After purification ...
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bioRxiv - Cell Biology 2022Quote: A carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) stock solution was made with the Vybrant™ CFDA-SE Cell Tracer Kit from Invitrogen immediately prior to use according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Cultured DRG neurons (18-48 hours) were loaded with 2.5 μM Fura-2-acetoxymethyl ester (Fura-2; Invitrogen, ThermoFisher Scientific, #F1221) in 0.01% pluronic F-127 (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: Cultured DRG neurons (18-48 hours) were loaded with 2.5 μM Fura-2-acetoxymethyl ester (Fura-2; Invitrogen, ThermoFisher Scientific, #F1221) in 0.01% pluronic F-127 (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... were carboxyl coupled to magnetic Luminex microplex carboxylated beads (Luminex Corporation) using NHS-ester linkages with Sulfo-NHS and EDC (Thermo Fisher), and then incubated with serum for 2 hours at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... 100 μg of anti-rabbit IgG was incubated with 8 μg of Alexa Fluor 750 N-Succinimidyl Ester (Thermo Fisher Scientific) in 100 mM NaHCO3 (pH 8.3 ...
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bioRxiv - Biochemistry 2021Quote: TE proteins were fluorescently labeled with the amine-reactive dye Alexa Fluor 647 NHS-Ester (Thermo Fisher Scientific, Rockford, IL, USA) as follows ...
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bioRxiv - Immunology 2021Quote: Splenocytes were resuspended at 106 cells/mL in 10% FBS in 1xPBS and stained with carboxyfluorescein succinimidyl ester (CFSE) (Invitrogen, #C34554) by rapidly mixing equal volume of cell suspension with 10 µM CFSE in 10% FBS in 1x PBS for 5 minutes at 37°C ...
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bioRxiv - Immunology 2021Quote: ... a T-cell proliferation assay was performed by incubating collected mice spleen cells for 7 min at RT with 5 μM Carboxyfluorescein succinimidyl ester (CFSE) (Life Technologies), staining was quenched with FCS and splenocytes cultured at 106 cells/well in 96-well plates for 5 days at 37°C in 5% CO2 with 3 μg/ml of rSp antigen ...
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bioRxiv - Biophysics 2020Quote: ... Oligonucleotides containing an amino-modified dT were labelled with either Alexa Fluor 488 or Alexa Fluor 594 NHS ester (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: Cell proliferation rates were estimated by tracing cells labelled with Carboxyfluorescein Succinimidyl Ester (CFSE) across generations and compared between populations using CellTrace™ CFSE Cell Proliferation Kit (C34554 Invitrogen) 23 ...
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bioRxiv - Cell Biology 2020Quote: Transfer of cells from the inner to the outer wells of co-cultures were studied as follows: young MPBs were labeled with CarboxyFluorescein succinimidyl ester (CFSE) (Thermo Fisher) following manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2021Quote: ... APRIL-Alexa488 and BSA-Alexa488 were prepared by conjugation of APRIL and BSA to Alexa Fluor 488 NHS ester (Thermo Fisher). Stained cells or beads were analyzed using a flow cytometer (LSR II ...