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Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: Plasmids (1 μg of sense and antisense guide RNAs + 3 μg donor for knockins) were diluted in 1 ml OptiMem (Gibco) and 20 μl of polyethylenimine (PEI ...
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bioRxiv - Developmental Biology 2021Quote: Donor embryos were injected at the 1-cell stage with 3-5nL of 1% Dextran-FITC 10,000 MW (Molecular Probes) and 0.05% Phenol Red (to see the solution ...
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bioRxiv - Cancer Biology 2022Quote: ... AF6-88.5.5.3, #17-5958-82), CD119 (1:100, 2E2, #13-1191-82), Armenian Hamster IgG isotype (1:100, eBio299Arm, #13488881) (Invitrogen); CD45 (1:400 ...
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bioRxiv - Developmental Biology 2022Quote: Organoids were treated immediately after PEG embedding for 3 days with neural differentiation medium comprised of a 1:1 mixture of neurobasal medium (GIBCO) and DMEM/F12 (GIBCO) ...
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bioRxiv - Pathology 2020Quote: ... 6-dihydroxy-2,4,5,7-tetraio-dospiro (isobenzofuran-1(3H),9[9H] xanthan)-3:1 dipotassium salt (50 mg/kg in 0.9% saline, Fisher Scientific) was injected retro-orbitally before catalyzing vessel injury with a 540 nm laser ...
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bioRxiv - Molecular Biology 2020Quote: ... To 1/3 of the sample 1/10 of the recommended amount of spike-in (ERCC RNA spike-in mix, Ambion) was added ...
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bioRxiv - Cell Biology 2021Quote: ... the media was replaced entirely with fresh retinal differentiation media (RDM) (DMEM:F12 3:1, 2% B27 supplement, MEM NEAA, 1× antibiotic, antimycotic (Thermo Fisher) and 1× GlutaMAX)) ...
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bioRxiv - Cell Biology 2020Quote: ... the media was changed to retinal differentiation medium (RDM;DMEM:F12 3:1, 2% B27 supplement, MEM NEAA, 1× antibiotic, antimycotic (Thermo Fisher) and 1× GlutaMAX) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL (10μM) reverse primer (ldh4_R, 5’-AATCACAGCAGCCCCTTG-3’) and 1 μL (1 U/μL) Platinum Taq DNA polymerase (Invitrogen, 10966-018) in a 50 μL total reaction volume ...
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bioRxiv - Neuroscience 2023Quote: ... the brains were placed in the secondary antibody solution containing 1xPBS/0.1% Triton X-100/3% donkey serum and a 1:1000 dilution of donkey anti-sheep 647 (A-21448, Thermofisher) for 10 days.
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bioRxiv - Cancer Biology 2023Quote: ... Buffy coat was mixed 1:2 with PBS and added onto a Ficoll gradient in a 3:1 ratio (Invitrogen). This was centrifuged at 2100 rpm for 25 minutes at RT (w/o brakes) ...
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bioRxiv - Systems Biology 2023Quote: ... The bottom tips were packed sequentially with three plugs of C18 membrane (3 M Empore) and mixed-mode ion exchange beads (SCX:SAX = 1:1, Applied Biosystems). The quantity of C18 membrane and mixed-mode ion exchange beads was adjusted based on the protein amount.
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bioRxiv - Microbiology 2024Quote: ... Tenfold dilutions were used to infect confluent Vero E6 cells in a 96-well plate in MEM (5% FBS, 1% penicillin-streptomycin, 1% kanamycin, 3% amphotericin B (Gibco)) at 37°C and 5% CO2 ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:3) (60) followed by secondary Alexa Fluor 488 goat α-mouse IgG antibody (Invitrogen / Thermo Fisher Scientific – 1:1,000).
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bioRxiv - Neuroscience 2023Quote: ... Media was partially renewed every 3-4 days with neuronal differentiation media 2 (NDM2: 1:1 DMEM/F12:NB (Gibco), glutamax (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... The secondary antibody in PBS supplemented with 3% BSA (Thermofisher, Mouse 800, 1:20000 and Thermofisher, Rabbit 680, 1:20000) was incubated in the dark for 1 hour on a rolling platform at room temperature ...
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bioRxiv - Physiology 2024Quote: ... WT and Mrp14-/- bone marrow neutrophils (2.5×106 mL-1) were resuspended in PBS and loaded with 3 µM Indo-1 AM (Invitrogen™) for 45min at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... the PBS or virus dilution in PBS was aspirated and 3 ml of overlay consisting of 1:1 2 x DMEM (DMEM high glucose, no sodium bicarbonate buffer powder [Gibco # 12-100-046] in 500 mL of RNase-free water ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in 1X PBS (3 x 10 m) before a 1 h incubation in Donkey anti-rabbit IgG (1:1000) (#A32754, Invitrogen) and Goat anti-chicken IgG (1:500 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with 1:10,000 Hoechst for 1-3 mins and mounted on glass slides with ProLong glass antifade mountant (Invitrogen, P36982).
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bioRxiv - Immunology 2022Quote: ... CD4+ CD44loCD62Lhi CD25− naive T cells were isolated from the spleen of CD45.1+ mice by cell sorting after positive enrichment for CD4+ cells using MACS LS columns (Miltenyi) and labelled with carboxyfluorescein diacetate succinimidyl ester (CFSE, ThermoFisher). CD4+YFP+ Treg cells were isolated from the spleen of Foxp3cre or PHD2ΔTreg mice by cell sorting ...
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bioRxiv - Immunology 2021Quote: ... DAPI was used for assessment of cell viability and in vitro B cell proliferation was assessed by flow cytometry after labeling of isolated CD43- naive mature B cells with CellTrace carboxyfluorescein succinimidyl ester (CFSE) (Invitrogen). In vitro derived PCs were stained with CellEvent Caspase 3/7-Green (ThermoFisher) ...
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bioRxiv - Cell Biology 2020Quote: Solution pH was adjusted to pH=8.0 with HEPES buffer and protein solution was mixed with an excess of Alexa647 NHS ester (Molecular probes) dissolved in DMSO ...
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bioRxiv - Bioengineering 2021Quote: ... 50 μL of 200 μM cDNAs were incubated at pH 8.5 with 50 equivalents of Alexa Fluor 488 NHS Ester (Life Technologies) in 30% DMF for 4 h ...
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bioRxiv - Microbiology 2020Quote: ... and the cells resuspended in 0.3 ml PBS containing 100 μM Carboxyfluorescein diacetate succinimidyl ester (CFDA-SE) (CellTrace™ CFSE, ThermoFisher) for 20 minutes at 37°C ...
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bioRxiv - Neuroscience 2020Quote: Staining the cells with the mitochondrial selective dye Rhod-2 AM (rhodamine-2 acetoxymethyl ester; Thermo Fisher Scientific, Darmstadt, Germany), allows for specific evaluation of mitochondrial calcium accumulation ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti-SeV NP (clone M73/2 a kind gift from Alan Portner – directly conjugated with Dylight 594 NHS Ester (ThermoFisher)) ...
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bioRxiv - Microbiology 2020Quote: PvRBP2b161-1454 and TfR1 were labelled with N-hydroxysuccinimide ester-activated DyLight 488 (DL488) and DyLight 594 (DL594) (Life Technologies) respectively ...
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bioRxiv - Biophysics 2022Quote: ... Matthias Mack (Signoret et al., 2000)MC-5 was fluorescently labeled using DyLight 650 NHS ester coupling kit (Thermo Fisher) with a dye:protein coupling ratio of 1.57:1.
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bioRxiv - Biophysics 2022Quote: Fluorescent labeling was performed by targeting histone amine groups of pre-assembled nucleosomes using Alexa Fluor 647 succinimidyl ester (ThermoFisher), following the previously reported strategy20 ...
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bioRxiv - Cell Biology 2022Quote: ... cells plated on glass bottom cell culture dish were loaded with 10 μmol/L Fura-2 acetoxymethyl ester (Molecular Probes) and 0.1% pluronic F-127 (Sigma ...
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bioRxiv - Bioengineering 2022Quote: The αEGFR-Fn3-DHFR2-CVIA protein was non-specifically labeled with DyLight™ 650 NHS Ester (Thermo Scientific, Cat: 62266) in PBS according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: ... Each probe is 18-22nt long with a 3’ end amine-modified nucleotide that allows directly couple to an NHS-ester dye according to the manufacturer’s instructions (Life Technologies). Dye-labeled probes were separated from the excess free dyes using the Qiagen Nucleotide Removal Columns ...
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bioRxiv - Biophysics 2020Quote: Wild-type and the three LXXAA mutants of TIF2NRID were amine-labelled with a fluorescent probe (Alexa 488 NHS Ester, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... target cells were labelled with 5 µM of carboxyfluorescein succinimidyl ester (CFSE) from a CFSE cell proliferation kit (Invitrogen, C34554) for 5 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: 100μl of reovirus particles (from 1013 particles/ml stock) were mixed with 0.4μl of Alexa488 or Alexa647 NHS Ester (8mM starting concentration) (Thermo Fisher Scientific) for 1h at room temperature (RT) ...
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bioRxiv - Cell Biology 2020Quote: Fluorescent microtubules were polymerized from 4 mg/ml porcine tubulin (80% unlabeled and 20% Alexa Fluor 647 NHS ester-labeled; Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The crosslinking reaction was then performed using the NHS-ester-based and CID-cleavable reagent disuccinimidyl sulfoxide (DSSO; Thermo Fisher) (57 ...
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bioRxiv - Microbiology 2021Quote: ... reovirus particles were diluted to 6×1012 particles/ml in freshly prepared 50 mM sodium bicarbonate buffer and incubated with 20 μM Alexa Fluor 647 succinimidyl ester dye (Invitrogen) at room temperature for 90 min ...
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bioRxiv - Microbiology 2021Quote: Fluorescent labeling of T4 and φ29 phages was accomplished by adding 475μl phage with 25μl Alexa Fluor™ 488 NHS-Ester (Thermo Fisher) at a final concentration of 0.05 mg/ml (10mM) ...
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bioRxiv - Immunology 2023Quote: ... which was followed by an overnight incubation at 4°C with N-hydroxysuccinimide ester derivatives of AF647 (Thermo Fisher Scientific) present in two-fold molar excess.
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bioRxiv - Immunology 2022Quote: ... MBP peptide NPVVHFFKNIVTPR and influenza hemagglutinin PRFVKQNTLRLAT peptide were labeled with Alexa Fluor 488 (Alexa488) tetrafluorophenyl ester (Invitrogen, Carlsbad, CA) and used as probe peptides for DP4.1 ...
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bioRxiv - Biophysics 2023Quote: Proteins were labelled by using the AF488-NHS ester according to the manufacturer’s protocol and purified from the excess of the dye by Zeba spin columns (Thermo Scientific), dialysis (Pur-A-Lyzer ...
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bioRxiv - Cancer Biology 2023Quote: Rhodamine tubulin was generated by labeling tubulin with X-rhodamine SE (5(and-6) carboxy-X-rhodamine succinimidyl ester) dye (ThermoFisher). Labeling stoichiometry was determined using a molar extinction coefficient of 115,000 M-1 cm-1 for tubulin and 78,000 M-1 cm-1 for X-rhodamine [58] ...
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bioRxiv - Cell Biology 2023Quote: HM sEV uptake by Caco-2 cells was performed after labeling EVs with carboxyfluorescein succinimidyl ester (CFSE; Thermo Fisher Scientific) [29] ...
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
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bioRxiv - Physiology 2022Quote: ... The mitochondria membrane potential was also assessed in cells stained with 50 nM tetramethyl rhodamine ethyl ester (TMRE, ThermoFisher, T669) and subsequently collected in HBSS containing 5 mM EDTA ...
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bioRxiv - Neuroscience 2022Quote: ... y229Gt (Tg(tnks1bp1:EGFP)) transgenic larvae were exposed to 250 nM tetramethylrhodamine ethyl ester perchlorate (TMRE, Thermo Fisher, Cat# T669) diluted in EM for 25 minutes while wrapped in foil ...
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bioRxiv - Biochemistry 2024Quote: ... LIG1 proteins were then fluorescently labeled using amine reactive NHS ester conjugated Alexa Fluor (AF)488 according to the manufacturer’s instructions (Thermo Fisher). The protein concentrations and labeling efficiency were finally determined by calculating the molar ratio of dye to protein within the solution by measuring absorbance at 280 nm and 488 nm.
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bioRxiv - Immunology 2024Quote: ... T-allo10 cells or control T-allo Teff cells were labeled with CellTrace carboxyfluorescein succinimidyl ester (CFSE; Thermo Fisher Scientific) for 5 to 8 minutes at room temperature ...